المؤلفون: de Faria, Isaque J.S., Imler, Jean-Luc, Marques, João T.

المصدر: STAR Protocols ; volume 4, issue 1, page 102033 ; ISSN 2666-1667

مصطلحات موضوعية: General Immunology and Microbiology, General Biochemistry, Genetics and Molecular Biology, General Neuroscience

الإتاحة: https://doi.org/10.1016/j.xpro.2022.102033Test
https://api.elsevier.com/content/article/PII:S2666166722009133?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2666166722009133?httpAccept=text/plainTest

المؤلفون: De Faria, Isaque J.S., Aguiar, Eric R.G.R., Proveti Olmo, R. (Roenick), Alves da Silva, J. (Juliana), Daeffler, L. (Laurent), Carthew, Richard W., Imler, J. (Jean-Luc), Marques, João T.

مصطلحات موضوعية: Sciences du Vivant [q-bio]/Immunologie

الوصف: dsRNA sensing triggers antiviral responses against RNA and DNA viruses in diverse eukaryotes. In Drosophila, Invertebrate iridescent virus 6 (IIV-6), a large DNA virus, triggers production of small interfering RNAs (siRNAs) by the dsRNA sensor Dicer-2. Here, we show that host RNA polymerase II (RNAPII) bidirec- tionally transcribes specific AT-rich regions of the IIV-6 DNA genome to generate dsRNA. Both replicative and naked IIV-6 genomes trigger production of dsRNA in Drosophila cells, implying direct sensing of invading DNA. Loquacious-PD, a Dicer-2 co-factor essential for the biogenesis of endogenous siRNAs, is dispensable for processing of IIV-6-derived dsRNAs, which suggests that they are distinct. Consistent with this finding, inhibition of the RNAPII co-factor P-TEFb affects the synthesis of endogenous, but not virus-derived, dsRNA. Altogether, our results suggest that a non-canonical RNAPII complex recognizes invading viral DNA to synthesize virus-derived dsRNA, which activates the antiviral siRNA pathway in Drosophila.

الإتاحة: https://linkinghub.elsevier.com/retrieve/pii/S2211124722007628Test

المؤلفون: Olmo, Roenick P., Todjro, Yaovi M.H., Aguiar, Eric R.G.R., de Almeida, João Paulo P., Ferreira, Flávia V., Armache, Juliana N., de Faria, Isaque J.S., Ferreira, Alvaro G.A., Amadou, Siad C.G., Silva, Ana Teresa S., de Souza, Kátia P.R., Vilela, Ana Paula P., Babarit, Antinea, Tan, Cheong H., Diallo, Mawlouth, Gaye, Alioune, Paupy, Christophe, Obame-Nkoghe, Judicaël, Visser, Tessa M., Koenraadt, Constantianus J.M., Wongsokarijo, Merril A., Cruz, Ana Luiza C., Prieto, Mariliza T., Parra, Maisa C.P., Nogueira, Maurício L., Avelino-Silva, Vivian, Mota, Renato N., Borges, Magno A.Z., Drumond, Betânia P., Kroon, Erna G., Recker, Mario, Sedda, Luigi, Marois, Eric, Imler, Jean Luc, Marques, João T.

المصدر: Nature Microbiology 8 (2023) 1 ; ISSN: 2058-5276

مصطلحات موضوعية: Life Science

الوصف: Aedes aegypti and A. albopictus mosquitoes are the main vectors for dengue virus (DENV) and other arboviruses, including Zika virus (ZIKV). Understanding the factors that affect transmission of arboviruses from mosquitoes to humans is a priority because it could inform public health and targeted interventions. Reasoning that interactions among viruses in the vector insect might affect transmission, we analysed the viromes of 815 urban Aedes mosquitoes collected from 12 countries worldwide. Two mosquito-specific viruses, Phasi Charoen-like virus (PCLV) and Humaita Tubiacanga virus (HTV), were the most abundant in A. aegypti worldwide. Spatiotemporal analyses of virus circulation in an endemic urban area revealed a 200% increase in chances of having DENV in wild A. aegypti mosquitoes when both HTV and PCLV were present. Using a mouse model in the laboratory, we showed that the presence of HTV and PCLV increased the ability of mosquitoes to transmit DENV and ZIKV to a vertebrate host. By transcriptomic analysis, we found that in DENV-infected mosquitoes, HTV and PCLV block the downregulation of histone H4, which we identify as an important proviral host factor in vivo.

وصف الملف: application/pdf

العلاقة: https://edepot.wur.nl/585777Test; https://research.wur.nl/en/publications/mosquito-vector-competence-for-dengue-is-modulated-by-insect-specTest

الإتاحة: https://doi.org/10.1038/s41564-022-01289-4Test
https://research.wur.nl/en/publications/mosquito-vector-competence-for-dengue-is-modulated-by-insect-specTest