المؤلفون: da Silva, Jean Lucas Gutknecht, Viana, Altevir Rossato, Passos, Daniela Ferreira, Krause, Luciana Maria Fontanari, Miron, Vanessa Valéria, Schetinger, Maria Rosa Chitolina, Pillat, Micheli Mainardi, Palma, Taís Vidal, Leal, Daniela Bitencourt Rosa

المصدر: Purinergic Signalling; Dec2023, Vol. 19 Issue 4, p633-650, 18p

مستخلص: ATP and adenosine exert pivotal roles in the development, maintenance, and metastatic spreading of melanoma. The action of such key melanoma tumor microenvironment (TME) constituents might be complementary or opposed, and their effects are not exclusive to immune cells but also to other host cells and tumor cells. The effects of ATP are controlled by the axis CD39/73, resulting in adenosine, the main actor in the TME, and A2A is the crucial mediator of its effects. We evaluated ATP and adenosine signaling through A2A on B16F10 melanoma cells using istradefylline (IST) (antiparkinsonian A2A antagonist) and caffeine (CAF) treatments after exposure to ATP and adenosine. Adenosine increased melanoma cell viability and proliferation in a concentration-dependent manner. ATP increases viability only as a substrate by CD39 to produce adenosine. Both IST and CAF are toxic to B16F10 cells, but only IST potentialized paclitaxel-induced cytotoxic effects, even decreasing its IC50 value. IST positively modulated CD39 and CD73 expression. CD39 activity was increased, and E-ADA was reduced, indicating that the melanoma cells promoted compensatory feedback in the production and maintenance of adenosine levels. A2A antagonism by IST reduced the factors associated with malignancy, like migration, adhesion, colony formation, and the capacity to produce melanin. Moreover, IST significantly increases nitric oxide (NO) production, which correlates to a decline in melanoma cell viability by apoptotic events. Altogether, our results suggest that adenosine signaling through A2A is essential for B16F10 cells, and its inhibition by IST causes compensatory purinergic enzymatic modulations. Furthermore, IST is a promising therapy that provides new ways to improve current melanoma treatments. [ABSTRACT FROM AUTHOR]

: Copyright of Purinergic Signalling is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Jantsch, Matheus Henrique, Doleski, Pedro Henrique, Viana, Altevir Rossato, da Silva, Jean Lucas Gutknecht, Passos, Daniela Ferreira, Cabral, Fernanda Licker, Manzoni, Alessandra Guedes, Ebone, Renan da Silva, Soares, Ana Bárbara Uchoa, de Andrade, Cínthia Melazzo, Schetinger, Maria Rosa Chitolina, Leal, Daniela Bitencourt Rosa

المصدر: Biochemistry & Cell Biology; Oct2023, Vol. 101 Issue 5, p443-455, 13p

مصطلحات موضوعية: CLOPIDOGREL, ROOT-tubercles, MELANOMA, ADENOSINE diphosphate, BLOOD platelet activation, LACTATE dehydrogenase, BLOOD platelet aggregation, CORD blood

مستخلص: Metastatic melanoma is a very aggressive skin cancer. Platelets are constituents of the tumor microenvironment and, when activated, contribute to cancer progression, especially metastasis and inflammation. P2Y12 is an adenosine diphosphate receptor that triggers platelet activation. Inhibition of P2Y12 by clopidogrel bisulfate (CB) decreases platelet activation, which is also controlled by the extracellular concentration and the metabolism of purines by purinergic enzymes. We evaluated the effects of CB on the viability and proliferation of cultured B16-F10 cells. We also used a metastatic melanoma model with C57BL-6 mice to evaluate cancer development and purine metabolism modulation in platelets. B16-F10 cells were administered intraperitoneally to the mice. Two days later, the animals underwent a 12-day treatment with CB (30 mg/kg by gavage). We have found that CB reduced cell viability and proliferation in B16-F10 culture in 72 h at concentrations above 30 µm. In vivo, CB decreased tumor nodule counts and lactate dehydrogenase levels and increased platelet purine metabolism. Our results showed that CB has significant effects on melanoma progression. [ABSTRACT FROM AUTHOR]

: Copyright of Biochemistry & Cell Biology is the property of Canadian Science Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Urquhart, Carolina Gonzalez, Pinheiro, Ticiane da Rosa, da Silva, Jean Lucas Gutknecht, Leal, Daniela Bitencourt Rosa, Burgo, Thiago Augusto Lima, Iglesias, Bernardo Almeida, Santos, Roberto Christ Vianna

المصدر: Photodiagnosis and Photodynamic Therapy ; volume 42, page 103266 ; ISSN 1572-1000

مصطلحات موضوعية: Pharmacology (medical), Dermatology, Oncology, Biophysics

الإتاحة: https://doi.org/10.1016/j.pdpdt.2022.103266Test
https://api.elsevier.com/content/article/PII:S157210002200552X?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S157210002200552X?httpAccept=text/plainTest

المؤلفون: da Rosa Pinheiro, Ticiane, Dantas, Gabrielle Aguiar, da Silva, Jean Lucas Gutknecht, Leal, Daniela Bitencourt Rosa, da Silva, Ricardo Barreto, de Lima Burgo, Thiago Augusto, Santos, Roberto Christ Vianna, Iglesias, Bernardo Almeida

المصدر: Pharmaceutics; May2023, Vol. 15 Issue 5, p1511, 17p

مصطلحات موضوعية: ONYCHOMYCOSIS, CANDIDA albicans, METALLOPORPHYRINS, PORPHYRINS, ATOMIC force microscopy, MYCOSES

مستخلص: Onychomycosis is a prevalent nail fungal infection, and Candida albicans is one of the most common microorganisms associated with it. One alternative therapy to the conventional treatment of onychomycosis is antimicrobial photoinactivation. This study aimed to evaluate for the first time the in vitro activity of cationic porphyrins with platinum(II) complexes 4PtTPyP and 3PtTPyP against C. albicans. The minimum inhibitory concentration of porphyrins and reactive oxygen species was evaluated by broth microdilution. The yeast eradication time was evaluated using a time-kill assay, and a checkerboard assay assessed the synergism in combination with commercial treatments. In vitro biofilm formation and destruction were observed using the crystal violet technique. The morphology of the samples was evaluated by atomic force microscopy, and the MTT technique was used to evaluate the cytotoxicity of the studied porphyrins in keratinocyte and fibroblast cell lines. The porphyrin 3PtTPyP showed excellent in vitro antifungal activity against the tested C. albicans strains. After white-light irradiation, 3PtTPyP eradicated fungal growth in 30 and 60 min. The possible mechanism of action was mixed by ROS generation, and the combined treatment with commercial drugs was indifferent. The 3PtTPyP significantly reduced the preformed biofilm in vitro. Lastly, the atomic force microscopy showed cellular damage in the tested samples, and 3PtTPyP did not show cytotoxicity against the tested cell lines. We conclude that 3PtTPyP is an excellent photosensitizer with promising in vitro results against C. albicans strains. [ABSTRACT FROM AUTHOR]

: Copyright of Pharmaceutics is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: da Silva, Jean Lucas Gutknecht, Viana, Altevir Rossato, Passos, Daniela Ferreira, Krause, Luciana Maria Fontanari, Miron, Vanessa Valéria, Schetinger, Maria Rosa Chitolina, Pillat, Micheli Mainardi, Palma, Taís Vidal, Leal, Daniela Bitencourt Rosa

المساهمون: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

المصدر: Purinergic Signalling ; volume 19, issue 4, page 633-650 ; ISSN 1573-9538 1573-9546

مصطلحات موضوعية: Cell Biology, Cellular and Molecular Neuroscience, Molecular Biology

الإتاحة: https://doi.org/10.1007/s11302-022-09909-8Test

المؤلفون: Martins, Francisco Mainardi, Iglesias, Bernardo Almeida, Chaves, Otávio Augusto, da Silva, Jean Lucas Gutknecht, Leal, Daniela Bitencourt Rosa, Back, Davi Fernando

مصطلحات موضوعية: Crystal Structure, Experimental 3D Coordinates, Crystal System, Space Group, Cell Parameters, Crystallography, {[3-{[4-fluorophenyloxymethylidene]hydrazinylidene}methyl-4-oxyphenyl]methyl}triphenylphosphonium-dioxo-vanadiumv methanol solvate monohydrate

الوصف: Related Article: Francisco Mainardi Martins, Bernardo Almeida Iglesias, Otávio Augusto Chaves, Jean Lucas Gutknecht da Silva, Daniela Bitencourt Rosa Leal, Davi Fernando Back|2024|Dalton Trans.|53|8315|doi:10.1039/D4DT00464G ... : An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures. ...

وصف الملف: CIF

العلاقة: https://dx.doi.org/10.1039/D4DT00464GTest

الإتاحة: https://doi.org/10.5517/ccdc.csd.cc2j7zxp10.1039/D4DT00464GTest