المؤلفون: Ajit Kumar Bishoyi, Chita Ranjan Sahoo, Priyanka Samal, Nilima Priyadarsini Mishra, Bigyan Ranjan Jali, Mohd Shahnawaz Khan, Rabindra Nath Padhy

المصدر: Scientific Reports, Vol 14, Iss 1, Pp 1-15 (2024)

مصطلحات موضوعية: Green synthesis, Silver nanoparticles, Chromolaena odorata, Antimicrobial activities, Molecular docking, Medicine, Science

الوصف: Abstract This research investigates the biogenic synthesis of silver nanoparticles (AgNPs) using the leaf extract of Chromolaena odorata (Asteraceae) and their potential as antibacterial and antifungal agents. Characterization techniques like ultraviolet–visible, Fourier transform infrared (FTIR), Dynamic light scattering and zeta potential (DLS), X-ray diffraction (XRD), transmission electron microscopy (TEM), and field emission scanning electron microscopy and energy-dispersive X-ray spectroscopy (FESEM-EDX) confirmed the formation of spherical (AgNPs). UV–vis spectroscopy reaffirms AgNP formation with a peak at 429 nm. DLS and zeta potential measurements revealed an average size of 30.77 nm and a negative surface charge (− 0.532 mV). Further, XRD analysis established the crystalline structure of the AgNPs. Moreover, the TEM descriptions indicate that the AgNPs are spherical shapes, and their sizes ranged from 9 to 22 nm with an average length of 15.27 nm. The X-ray photoelectron spectroscopy (XPS) analysis validated the formation of metallic silver and elucidated the surface state composition of AgNPs. Biologically, CO-AgNPs showed moderate antibacterial activity but excellent antifungal activity against Candida tropicalis (MCC 1559) and Trichophyton rubrum (MCC 1598). Low MIC values (0.195 and 0.390 mg/mL) respectively, suggest their potential as effective antifungal agents. This suggests potential applications in controlling fungal infections, which are often more challenging to treat than bacterial infections. Molecular docking results validated that bioactive compounds in C. odorata contribute to antifungal activity by interacting with its specific domain. Further research could pave the way for the development of novel and safe antifungal therapies based on biogenic nanoparticles.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/85cffba252364017b3e7c57d42693a42Test

المؤلفون: Hyonam Park, Bohee Kim, Yuri Kang, Woonjung Kim

المصدر: Current Issues in Molecular Biology, Vol 46, Iss 3, Pp 2133-2143 (2024)

مصطلحات موضوعية: Psidium guajava, antimicrobial activities, tyrosinase, collagenase, trans-2-nonenal inhibition, Biology (General), QH301-705.5

الوصف: Guava (Psidium guajava) is a plant widely distributed in tropical and subtropical regions. Its leaves contain a large amount of physiological molecules such as flavonoid, sesquiterpene, triterpenoid, coumarin, alkaloid, and tannin molecules with antioxidative and anti-inflammatory effects. In this study, the use of concentrated P. guajava leaf extract molecules as a functional natural material was evaluated by confirming the extract’s antioxidative, antibacterial, tyrosinase activity inhibition, and collagenase activity inhibition effects and its trans-2-nonenal removal ability. As a result of the analysis of the antioxidant and antibacterial components of concentrated P. guajava leaf extract molecules through GC-MS, a large amount of aromatic hydrocarbon molecules were detected. When different concentrations of ethanol were used for extraction, the leaf extract concentrated with 70% ethanol showed the most effective active molecules. As a result of measuring DPPH radical scavenging activity, a concentration-dependent antioxidant activity was confirmed. The antioxidant activity tended to increase when the ethanol content used for extraction was increased. Molecules such as 2,4-di-tert-butylphenol, caryophyllene oxide, and γ-muurolene in P. guajava leaf extract concentrate appeared to have antibacterial activities against S. aureus bacteria known to cause atopy. As ethanol content increased, the inhibitory effect on tyrosinase activity was increased. In addition, when ethanol content was 50%, the concentrated leaf extract was able to remove trans-2-nonenal by 52.4%. As a result of determining the concentrated leaf extract’s collagenase inhibition activity, an inhibition rate close to that of ascorbic acid, a positive control, was confirmed. The concentrated guajava leaf extract molecules were confirmed to have whitening and wrinkle-improving functionality. Thus, the P. guajava leaf extract has high potential as a food and natural cosmetic material.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1467-3045/46/3/137Test; https://doaj.org/toc/1467-3037Test; https://doaj.org/toc/1467-3045Test

الوصول الحر: https://doaj.org/article/536f561a2dc24d46b3fda2e4f34e43adTest

المؤلفون: Akacha Usman Linus, Mohammed Hassan Shagal, Iliya Ibrahim Nkafamiya

المصدر: Traektoriâ Nauki, Vol 10, Iss 2, Pp 5001-5007 (2024)

مصطلحات موضوعية: musa acuminata calla, antimicrobial activities, stigmasterol, extraction, Science

الوصف: Banana blossom is traditionally used in northern Nigeria to treat a variety of ailments, including microbiological infections; reduces menstrual bleeding; aids in the treatment of diabetes, anaemia, and ulcers. Reduces anxiety, aids in weight loss, and produces an undetermined amount of filtrate decoction to facilitate conception. As a result, the study intends to isolate secondary metabolites to identify the bioactive compound(s) present in banana flowers. Based on the antimicrobial activity results, the highest inhibitory concentration for ethyl acetate extracts of the Banana blossom flower is 12.5 mg/ml, as indicated below. Compounds are isolated using column chromatography. Isolation was performed using an ethyl acetate extract of the banana blossom flower. The fractions from the column are subjected to TLC testing to determine the separation of the component combination using a thin stationary phase supported by an inert backing, resulting in BF16 fractions. The goal of TLC is to produce well-defined, well-separated spots. Each component has a retention factor (RF), which joins fractions with equal RF values. We extracted stigmasterol from Musa acuminata calla ethyl acetate extracts and analysed it using NMR spectroscopy and FT-IR.

وصف الملف: electronic resource

العلاقة: https://pathofscience.org/index.php/ps/article/view/3025Test; https://doaj.org/toc/2413-9009Test

الوصول الحر: https://doaj.org/article/f8684664fe314d17953dcfbfba94ab9dTest

المؤلفون: Mohamed A.M. Abdel Reheim, Ibrahim S. Abdel Hafiz, Hala M. Reffat, Hend S. Abdel Rady, Ihsan A. Shehadi, Huda R.M. Rashdan, Abdelfattah Hassan, Aboubakr H. Abdelmonsef

المصدر: Heliyon, Vol 10, Iss 13, Pp e33160- (2024)

مصطلحات موضوعية: 4-Acetyl-1,3-diphenyl-1H-pyrazole-5(4H)-ole, Pyrazole, Antibiotic resistance, Antimicrobial activities, DNA gyrase, Molecular docking, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: In the present work, two hybrid series of pyrazole-clubbed pyrimidine and pyrazole-clubbed thiazole compounds 3–21 from 4-acetyl-1,3-diphenyl-1H-pyrazole-5(4H)-ole 1 were synthesized as novel antimicrobial agents. Their chemical structures were thoroughly elucidated in terms of spectral analyses such as IR, 1H NMR, 13C NMR and mass spectra. The compounds were in vitro evaluated for their antimicrobial efficiency against various standard pathogen strains, gram -ive bacteria (Pseudomonas aeruginosa, Klebsiella pneumonia), gram + ive bacteria (MRSA, Bacillus subtilis), and Unicellular fungi (Candida albicans) microorganisms. The ZOI results exhibited that most of the tested molecules exhibited inhibition potency from moderate to high. Where compounds 7, 8, 12, 13 and 19 represented the highest inhibition potency against most of the tested pathogenic microbes comparing with the standard drugs. In addition, the MIC results showed that the most potent molecules 7, 8, 12, 13 and 19 showed inhibition effect against most of the tested microbes at low concentration. Moreover, the docking approach of the newly synthesized compounds against DNA gyrase enzyme was performed to go deeper into their molecular mechanism of antimicrobial efficacy. Further, computational investigations to calculate the pharmacokinetics parameters of the compounds were performed. Among them 7, 8, 12, 13 and 19 are the most potent compounds revealed the highest inhibition efficacy against most of the tested pathogenic microbes comparing with the standard drugs.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844024091916Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/b4ce63c937724c4f9f32793e885d203eTest

المؤلفون: Chhangte Vanlalveni, Vanlalhruaii Ralte, Hlawncheu Zohmingliana, Shikhasmita Das, Jasha Momo H. Anal, Samuel Lallianrawna, Samuel Lalthazuala Rokhum

المصدر: Heliyon, Vol 10, Iss 11, Pp e32333- (2024)

مصطلحات موضوعية: Green synthesis, Silver nanoparticles, Microbes-assisted synthesis, Antimicrobial activities, Extracellular, Intracellular, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: In recent decades, biosynthesis of metal and (or) metal oxide nanoparticles using microbes is accepted as one of the most sustainable, cost-effective, robust, and green processes as it does not encompass the usage of largely hazardous chemicals. Accordingly, numerous simple, inexpensive, and environmentally friendly approaches for the biosynthesis of silver nanoparticles (AgNPs) were reported using microbes avoiding conventional (chemical) methods. This comprehensive review detailed an advance made in recent years in the microbes-mediated biosynthesis of AgNPs and evaluation of their antimicrobial activities covering the literature from 2015-till date. It also aimed at elaborating the possible effect of the different phytochemicals, their concentrations, extraction temperature, extraction solvent, pH, reaction time, reaction temperature, and concentration of precursor on the shape, size, and stability of the synthesized AgNPs. In addition, while trying to understand the antimicrobial activities against targeted pathogenic microbes the probable mechanism of the interaction of produced AgNPs with the cell wall of targeted microbes that led to the cell's reputed and death have also been detailed. Lastly, this review detailed the shape and size-dependent antimicrobial activities of the microbes-mediated AgNPs and their enhanced antimicrobial activities by synergetic interaction with known commercially available antibiotic drugs.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844024083646Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/aee403470eef46128b3706304e32274aTest

المؤلفون: Elizabeth Bosede Aladejana, Olusesan Adeyemi Adelabu, Adebowale Emmanuel Aladejana, Sizwe Innocent Ndlovu

المصدر: Pharmacological Research - Modern Chinese Medicine, Vol 11, Iss , Pp 100432- (2024)

مصطلحات موضوعية: Alternative medicine, Antimicrobial activities, Diabetes, Infections, Medicinal plants, Polyherbal formulations, Other systems of medicine, RZ201-999, Therapeutics. Pharmacology, RM1-950

الوصف: Introduction: Diabetes mellitus poses major health risks due to increased vulnerability to infections stemming from weakened immune systems and physiological complications. With antimicrobial resistance worsening prognosis, diabetic infections affecting multiple body systems present critical treatment challenges. This review examines the antimicrobial properties of alternative medicines used in the management of diabetic infections and addresses the apparent research gap in this area. Method: A comprehensive literature search was conducted on databases (PubMed, Scopus, ScienceDirect, and Google Scholar) using specified keywords. Exclusion criteria removed non-relevant articles, resulting in the selection of articles published between 2012 and 2023, focusing on medicinal plants, polyherbal formulations, and antimicrobial effects against pathogens associated with diabetic infections. Results: The study revealed a significant research gap regarding the use of alternative medicines for diabetic infection treatment, as only 14 articles published between 2012 and 2023 were relevant to this review. Selected articles primarily addressed wound infections (39 %), diabetic foot ulcers (28 %), foot infections (17 %), urinary tract 11 %), and skin infections (5 %). Commonly used medicinal plants include Curcuma longa, Azadirachta indica, Zingiber officinale, and Glycyrrhiza glabra among others. The alternative medicines demonstrated significant antimicrobial potential against common microorganisms associated with diabetic infections, especially Gram-positive bacteria. Discussion: This review shows the potential of alternative medicines in the management of diabetic infections. This efficacy was attributed to diverse secondary metabolites present in medicinal plants, offering promising prospects for novel antimicrobial agents. Given the susceptibility of diabetic patients to microbial infections, further investigation into the efficacy of medicinal plants for the treatment of infections in diabetic patients is essential. Further research is also imperative to validate and standardize these therapies and conduct clinical trials to evaluate their safety and efficacy in diabetic infection management.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2667142524000757Test; https://doaj.org/toc/2667-1425Test

الوصول الحر: https://doaj.org/article/3fe35738208045bbafc6c6774c2a0cd8Test

المؤلفون: Muhammad Hubab, Mohammad A. Al-Ghouti

المصدر: Biotechnology Reports, Vol 42, Iss , Pp e00837- (2024)

مصطلحات موضوعية: Metal-organic frameworks (MOFs), Antimicrobial activities, Antifungal, Antibacterial, Antiviral, Biotechnology, TP248.13-248.65

الوصف: Microbial infections, particularly those caused by antibiotic-resistant pathogens, pose a critical global health threat. Metal-Organic Frameworks (MOFs), porous crystalline structures built from metal ions and organic linkers, initially developed for gas adsorption, have emerged as promising alternatives to traditional antibiotics. This review, covering research up to 2023, explores the potential of MOFs and MOF-based materials as broad-spectrum antimicrobial agents against bacteria, viruses, fungi, and even parasites. It delves into the historical context of antimicrobial agents, recent advancements in MOF research, and the diverse synthesis techniques employed for their production. Furthermore, the review comprehensively analyzes the mechanisms of action by which MOFs combat various microbial threats. By highlighting the vast potential of MOFs, their diverse synthesis methods, and their effectiveness against various pathogens, this study underscores their potential as a novel solution to the growing challenge of antibiotic resistance.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2215017X24000109Test; https://doaj.org/toc/2215-017XTest

الوصول الحر: https://doaj.org/article/0c92c23578344a0a8ee24a21890311abTest

المؤلفون: Maolu Zhang, Shuqian Lin, Lianquan Han, Jiaming Zhang, Shaoning Liu, Xiuzhen Yang, Ruiming Wang, Xiaohui Yang, Yunpeng Yi

المصدر: Frontiers in Pharmacology, Vol 15 (2024)

مصطلحات موضوعية: halicin, antimicrobial activities, ames test, safety evaluation, Clostridium perfringens, Therapeutics. Pharmacology, RM1-950

الوصف: Halicin, the first antibacterial agent discovered by artificial intelligence, exerts broad-spectrum antibacterial effects and has a unique structure. Our study found that halicin had a good inhibitory effect on clinical isolates of drug-resistant strains and Clostridium perfringens (C. perfringens). The safety of halicin was evaluated by acute oral toxicity, genotoxicity and subchronic toxicity studies. The results of acute toxicity test indicated that halicin, as a low-toxicity compound, had an LD50 of 2018.3 mg/kg. The results of sperm malformation, bone marrow chromosome aberration and cell micronucleus tests showed that halicin had no obvious genotoxicity. However, the results of the 90-day subchronic toxicity test indicated that the test rats exhibited weight loss and slight renal inflammation at a high dose of 201.8 mg/kg. Teratogenicity of zebrafish embryos showed that halicin had no significant teratogenicity. Analysis of intestinal microbiota showed that halicin had a significant effect on the intestinal microbial composition, but caused a faster recovery. Furthermore, drug metabolism experiments showed that halicin was poorly absorbed and quickly eliminated in vivo. Our study found that halicin had a good therapeutic effect on intestinal infection model of C. perfringens. These results show the feasibility of developing oral halicin as a clinical candidate drug for treating intestinal infections.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fphar.2024.1389293/fullTest; https://doaj.org/toc/1663-9812Test

الوصول الحر: https://doaj.org/article/7b8fc649ca574bf081fae33acd459be2Test

المؤلفون: Mohammed Mahbubul Matin, Ajoy Kumer, Akhel Chandro, Shopnil Akash, Unesco Chakma

المصدر: Orbital: The Electronic Journal of Chemistry, Vol 16, Iss 1 (2024)

مصطلحات موضوعية: ADMET, Antimicrobial activities, DFT docking, Molecular dynamics, Rhamnopyranose esters, Science, Chemistry, QD1-999

الوصف: The "Black and White Fungus" is a very infrequently developing pathogen with a high fatality rate that has prompted widespread public health concern during the period of the COVID-19 pandemic. This pathogenic fungus may be widely distributed in nature, in plants, and in deteriorating fruits and vegetables because of its widespread nature. Numerous sugar molecules, such as glucopyranoside and glucofuranose, have been reported to have significant antibacterial, antifungal, and antiviral activity, and they were also revealed to be able to inhibit multidrug-resistant microorganisms. The recent black fungus epidemic was extremely serious in India, combined with COVID-19, which contributed to the high mortality impact and deterioration of the situation due to the unavailability of effective treatments. So, rhamnopyranose type derivatives 1–9 were studied against the proteins associated with black and white fungi such as Mycolicibacterium smegmatis (PDB ID 7D6X), Rhizomucor miehei (PDB ID 4WTP), Candida auris (PDB ID 6U8J), and Aspergillus luchuensis (PDB 1BK1). These compounds exhibited favorable physical and biochemical scores, as well as appropriate ADMET metrics, among other characteristics. Following the molecular docking procedure, it was found that 1–9 had the highest binding affinity in most cases, (> -6.00 kcal/mol), while compound 9 had outstanding binding affinity against Rhizomucor miehei (-8.7 kcal/mol) and against Mycolicibacterium smegmatis (-8.2 kcal/mol). In addition, the binding affinity against white fungus is also outstanding. This time, compounds 8 and 9 had better binding energy, which is -7.8 kcal/mol against Aspergillus luchuensis (1BK1) and -7.6 kcal/mol against Candida auris (6U8J). Finally, the molecular dynamics simulation at 100 ns has proved that they are stable for new medication development. Among the derivatives 1–9, ligands 8 and 9 exhibited potential medicinal characteristics when all of the data were considered.

وصف الملف: electronic resource

العلاقة: https://periodicos.ufms.br/index.php/orbital/article/view/19351Test; https://doaj.org/toc/1984-6428Test

الوصول الحر: https://doaj.org/article/4fd1f77d8d2d4a4e972a3b2a7110ddfbTest

المؤلفون: Uhrinová Anna, Maľučká Lucia Ungvarská

المصدر: European Pharmaceutical Journal, Vol 70, Iss s2, Pp 45-47 (2024)

مصطلحات موضوعية: ophiocordyceps sinensis, dpph radical, silver ions, antioxidant and antimicrobial activities, Therapeutics. Pharmacology, RM1-950

الوصف: Mushrooms from the genus Cordyceps are characterized by a wide range of biological effects due to the diverse amount of substances contained in them and are an important source of bioactive compounds. In China, mushrooms of the genus Cordyceps have been used as a medicinal preparation of traditional Chinese medicine for centuries. So far, a considerable number of studies have been conducted that focused on analyzing the effects of Cordyceps, which include their antioxidant, antibacterial, immunomodulatory, antidiabetic, antitumor, and many other effects. The ability of fungi to form complexes with various metals is also interesting. It is believed that polysaccharides are the main component of the extracts involved in the complexation with metals, after which their biological effects are improved and deepened. The work deals with the comparison of the antioxidant and antibacterial effects of Cordyceps extracts with extracts of this mushroom enriched with silver ions. Based on scientific studies, it is assumed that there is a complexation between the chemical compounds of the extracts and silver.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2453-6725Test

الوصول الحر: https://doaj.org/article/13e6f47094a74a3db6a001c3b5813de5Test