المؤلفون: Huimin Cheng, Xixi Zhang, Xiao Li, Jack Ng

المصدر: New Journal of Physics, Vol 26, Iss 2, p 023019 (2024)

مصطلحات موضوعية: conservative force, non-conservative force, Mie particle, acoustic tweezers, acoustic trapping, Science, Physics, QC1-999

الوصف: A general acoustic force field can be decomposed into a conservative gradient force (GF) and a non-conservative scattering force (SF), which have very different physical and mathematical properties. However, the profiles of such forces for Mie particles are unknown, let alone their underlying physics. Here, by using a fast Fourier transform approach, we calculated the GF and SF for spherical particle of various sizes and various incident waves. For the same focused incident waves, the normalized GF and SF are similar for different particle sizes, while the total force can be quite different owing to the varying relative strength between the GF and SF. GF and SF possess symmetries that are not found in the incident waves, indicating that these physically and mathematically distinct forces have symmetries that are hidden from the beam profile. For a vortex beam carrying a well-defined topological charge, acoustic forces alone cannot trap particles.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1367-2630Test

الوصول الحر: https://doaj.org/article/bd25be450dfa4fd589a8a3c9e69683f8Test

المؤلفون: Vi-hung Tsan, Daniel Fan, Sabina Caneva, Carlas S. Smith, Gerard J. Verbiest

المصدر: HardwareX, Vol 14, Iss , Pp e00428- (2023)

مصطلحات موضوعية: Acoustic trapping, Acoustic force spectroscopy, Acoustophoretic, Microfluidics, Low-cost, Science (General), Q1-390

الوصف: A low-cost glass-based microfluidic flow cell with a piezo actuator is built using off-the-shelf parts (total cost €9 per device) to apply acoustophoretic force on polystyrene micro-beads. The main challenge in the fabrication of these devices was to ensure their leak tightness, which we solved using double-sided tape and nail polish. Beads with 1.5 μm diameter flowing in a 100 μm deep channel were trapped at 7.5 MHz using a 23.7 peak-to-peak voltage (Vpp) sinusoidal input. The trap located at 50 ± 0.1 μm depth was measured to have a stiffness of approximately 0.6 pN/μm. With this simple device we can trap and control the axial position of micrometer scale objects, which allows for the manipulation of beads and cells. We intend to use the device for force spectroscopy on micro-bead tethered DNA. This can be combined with super-resolution imaging techniques to study mechanics and binding of protein structures along a DNA strand as a function of induced tension.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2468067223000354Test; https://doaj.org/toc/2468-0672Test

الوصول الحر: https://doaj.org/article/2df9fc69fd7e4821b64c50d14b54e773Test

المؤلفون: Jeongmin Heo, Jun Hong Park, Hyo Jun Kim, Kisoo Pahk, Ki Joo Pahk

المصدر: Ultrasonics Sonochemistry, Vol 96, Iss , Pp 106435- (2023)

مصطلحات موضوعية: High intensity focused ultrasound, Boiling histotripsy, Acoustic cavitation, Sonothrombolysis, Acoustic net, Acoustic trapping, Chemistry, QD1-999, Acoustics. Sound, QC221-246

الوصف: Whilst sonothrombolysis is a promising and noninvasive ultrasound technique for treating blood clots, bleeding caused by thrombolytic agents used for dissolving clots and potential obstruction of blood flow by detached clots (i.e., embolus) are the major limitations of the current approach. In the present study, a new sonothrombolysis method is proposed for treating embolus without the use of thrombolytic drugs. Our proposed method involves (a) generating a spatially localised acoustic radiation force in a blood vessel against the blood flow to trap moving blood clots (i.e., generation of an acoustic net), (b) producing acoustic cavitation to mechanically destroy the trapped embolus, and (c) acoustically monitoring the trapping and mechanical fractionation processes. Three different ultrasound transducers with different purposes were employed in the proposed method: (1) 1-MHz dual focused ultrasound (dFUS) transducers for capturing moving blood clots, (2) a 2-MHz High Intensity Focused Ultrasound (HIFU) source for fractionating blood clots and (3) a passive acoustic emission detector with broad bandwidth (10 kHz to 20 MHz) for receiving and analysing acoustic waves scattered from a trapped embolus and acoustic cavitation. To demonstrate the feasibility of the proposed method, in vitro experiments with an optically transparent blood vessel-mimicking phantom filled with a blood mimicking fluid and a blood clot (1.2 to 5 mm in diameter) were performed with varying the dFUS and HIFU exposure conditions under various flow conditions (from 1.77 to 6.19 cm/s). A high-speed camera was used to observe the production of acoustic fields, acoustic cavitation formation and blood clot fragmentation within a blood vessel by the proposed method. Numerical simulations of acoustic and temperature fields generated under a given exposure condition were also conducted to further interpret experimental results on the proposed sonothrombolysis. Our results clearly showed that fringe pattern-like acoustic pressure fields (fringe width of 1 mm) produced in a blood vessel by the dFUS captured an embolus (1.2 to 5 mm in diameter) at the flow velocity up to 6.19 cm/s. This was likely to be due to the greater magnitude of the dFUS-induced acoustic radiation force exerted on an embolus in the opposite direction to the flow in a blood vessel than that of the drag force produced by the flow. The acoustically trapped embolus was then mechanically destructed into small pieces of debris (18 to 60 μm sized residual fragments) by the HIFU-induced strong cavitation without damaging the blood vessel walls. We also observed that acoustic emissions emitted from a blood clot captured by the dFUS and cavitation produced by the HIFU were clearly distinguished in the frequency domain. Taken together, these results can suggest that our proposed sonothrombolysis method could be used as a promising tool for treating thrombosis and embolism through capturing and destroying blood clots effectively.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S1350417723001475Test; https://doaj.org/toc/1350-4177Test

الوصول الحر: https://doaj.org/article/d91e7932df2a47d18dcb5f91f235fd0dTest

المؤلفون: Wrede, Paul, Aghakhani, Amirreza, Bozuyuk, Ugur, Yildiz, Erdost, Sitti, Metin, id_orcid:0 000-0001-8249-3854

المصدر: ACS Applied Materials & Interfaces, 15 (45)

مصطلحات موضوعية: acoustic manipulation, hollow microparticles, acoustic trapping, focusedultrasound, microrobotics, particle manipulation, ultrasound imaging, microbubbles

الوصف: Microparticle manipulation and trapping play pivotal roles in biotechnology. To achieve effective manipulation within fluidic flow conditions and confined spaces, it is necessary to consider the physical properties of microparticles and the types of trapping forces applied. While acoustic waves have shown potential for manipulating microparticles, the existing setups involve complex actuation mechanisms and unstable microbubbles. Consequently, the need persists for an easily deployable acoustic actuation setup with stable microparticles. Here, we propose the use of hollow borosilicate microparticles possessing a rigid thin shell, which can be efficiently trapped and manipulated using a single-lens focused ultrasound (FUS) transducer under physiologically relevant flow conditions. These hollow microparticles offer stability and advantageous acoustic properties. They can be scaled up and mass-produced, making them suitable for systemic delivery. Our research demonstrates the successful trapping dynamics of FUS within circular tubings of varying diameters, validating the effectiveness of the method under realistic flow rates and ultrasound amplitudes. We also showcase the ability to remove hollow microparticles by steering the FUS transducer against the flow. Furthermore, we present potential biomedical applications, such as active cell tagging and navigation in bifurcated channels as well as ultrasound imaging in mouse cadaver liver tissue. ; ISSN:1944-8244 ; ISSN:1944-8252

وصف الملف: application/application/pdf

العلاقة: info:eu-repo/semantics/altIdentifier/wos/001105543000001; http://hdl.handle.net/20.500.11850/645024Test

الإتاحة: https://doi.org/20.500.11850/645024Test
https://doi.org/10.3929/ethz-b-000645024Test
https://doi.org/10.1021/acsami.3c11656Test
https://hdl.handle.net/20.500.11850/645024Test

المؤلفون: Mia Kvåle Løvmo, Simon Moser, Gregor Thalhammer-Thurner, Monika Ritsch-Marte

المصدر: Frontiers in Physics, Vol 10 (2022)

مصطلحات موضوعية: 3D acoustic trapping, high-NA, microfluidics, acoustic torque, sustained rotation, reorientation, Physics, QC1-999

الوصف: In the life sciences, there has been growing awareness that the traditional 2D cell culture model has its limitations in advancing our understanding of the mechanisms that underlie cell behavior, as the behavior and response of cells depend on the 3D microenvironment. Studying models such as suspended cell clusters and organoids is a step toward closing the gap between in vitro and in vivo studies. The fact that sample confinement and contact with surfaces have an impact on cells creates a need for contact-less tools for the inspection of live biological samples. Recently, we developed an acoustofluidic chip to trap and manipulate sub-millimeter-sized biological samples, and here, we demonstrate that this device can be adapted to support high-resolution imaging and illumination scanning for multi-view image acquisition. After coupling acoustic bulk waves into a microfluidic chip, the sample is levitated by an optically transparent transducer in the vertical direction. Two orthogonal side-transducers give additional control over the sample. By tuning the relative strengths of the three transducers and thus inducing an acoustic torque, we can transiently rotate the sample into various orientations for image acquisition. Under different operating conditions, exciting other modes, we can also induce sustained rotation of samples by means of other torque contributions and around axes perpendicular to the imaging axis, which is important to avoid “missing cone” artifacts in the tomographic reconstruction of the sample. We will discuss the modifications to our previously established device that were necessary to comply with the requirements for high-NA imaging and high-NA illumination. We provide a characterization of the performance and show examples of rotation and reorientation of biological samples, such as large pollen grains and cancer spheroids.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fphy.2022.940115/fullTest; https://doaj.org/toc/2296-424XTest

الوصول الحر: https://doaj.org/article/54404ca933ee442c997ac73c9a408438Test

المؤلفون: Daan Paget, Antonio Checa, Benedikt Zöhrer, Raphael Heilig, Mayooran Shanmuganathan, Raman Dhaliwal, Errin Johnson, Maléne Møller Jørgensen, Rikke Bæk, Oxford Acute Myocardial Infarction Study (OxAMI), Craig E. Wheelock, Keith M. Channon, Roman Fischer, Daniel C. Anthony, Robin P. Choudhury, Naveed Akbar

المصدر: Journal of Extracellular Biology, Vol 1, Iss 11, Pp n/a-n/a (2022)

مصطلحات موضوعية: acoustic trapping, human, immunoaffinity capture, omics, plasma, precipitation, Cytology, QH573-671

الوصف: Abstract Plasma extracellular vesicle (EV) number and composition are altered following myocardial infarction (MI), but to properly understand the significance of these changes it is essential to appreciate how the different isolation methods affect EV characteristics, proteome and sphingolipidome. Here, we compared plasma EV isolated from platelet‐poor plasma from four healthy donors and six MI patients at presentation and 1‐month post‐MI using ultracentrifugation (UC), polyethylene glycol precipitation, acoustic trapping, size‐exclusion chromatography (SEC) and immunoaffinity capture. The isolated EV were evaluated by Nanoparticle Tracking Analysis (NTA), Western blot, transmission electron microscopy (TEM), an EV‐protein array, untargeted proteomics (LC‐MS/MS) and targeted sphingolipidomics (LC‐MS/MS). The application of the five different plasma EV isolation methods in patients presenting with MI showed that the choice of plasma EV isolation method influenced the ability to distinguish elevations in plasma EV concentration following MI, enrichment of EV‐cargo (EV‐proteins and sphingolipidomics) and associations with the size of the infarct determined by cardiac magnetic resonance imaging 6 months post‐MI. Despite the selection bias imposed by each method, a core of EV‐associated proteins and lipids was detectable using all approaches. However, this study highlights how each isolation method comes with its own idiosyncrasies and makes the comparison of data acquired by different techniques in clinical studies problematic.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2768-2811Test

الوصول الحر: https://doaj.org/article/44d6039664fe46df920cb4c07f32c95fTest

المؤلفون: Chen, Xiaoyang, Lam, Kwok Ho, Chen, Ruimin, Chen, Zeyu, Yu, Ping, Chen, Zhongping, Shung, K Kirk, Zhou, Qifa

المصدر: Biotechnology and Bioengineering. 114(11)

مصطلحات موضوعية: Data Management and Data Science, Information and Computing Sciences, Physical Sciences, Classical Physics, Acoustics, Cell Separation, Equipment Design, Equipment Failure Analysis, Micromanipulation, Reproducibility of Results, Sensitivity and Specificity, Transducers, Ultrasonography, acoustic trapping, ultrahigh frequency ultrasonic transducer, adjustable multi-scale tweezers, microparticle trapping and manipulation, "piezoelectric actuator" model, “piezoelectric actuator” model, Biotechnology

الوصف: This paper reports the fabrication, characterization, and microparticle manipulation capability of an adjustable multi-scale single beam acoustic tweezers (SBAT) that is capable of flexibly changing the size of "tweezers" like ordinary metal tweezers with a single-element ultrahigh frequency (UHF) ultrasonic transducer. The measured resonant frequency of the developed transducer at 526 MHz is the highest frequency of piezoelectric single crystal based ultrasonic transducers ever reported. This focused UHF ultrasonic transducer exhibits a wide bandwidth (95.5% at -10 dB) due to high attenuation of high-frequency ultrasound wave, which allows the SBAT effectively excite with a wide range of excitation frequency from 150 to 400 MHz by using the "piezoelectric actuator" model. Through controlling the excitation frequency, the wavelength of ultrasound emitted from the SBAT can be changed to selectively manipulate a single microparticle of different sizes (3-100 μm) by using only one transducer. This concept of flexibly changing "tweezers" size is firstly introduced into the study of SBAT. At the same time, it was found that this incident ultrasound wavelength play an important role in lateral trapping and manipulation for microparticle of different sizes. Biotechnol. Bioeng. 2017;114: 2637-2647. © 2017 Wiley Periodicals, Inc.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/8t51r1zzTest

المؤلفون: Cheng, Huimin, Zhang, Xixi, Li, Xiao, Ng, Jack

مصطلحات موضوعية: Conservative force, Non-conservative force, Mie particle, Acoustic tweezers, Acoustic trapping

الوصف: A general acoustic force field can be decomposed into a conservative gradient force (GF) and a non-conservative scattering force (SF), which have very different physical and mathematical properties. However, the profiles of such forces for Mie particles are unknown, let alone their underlying physics. Here, by using a fast Fourier transform approach, we calculated the GF and SF for spherical particle of various sizes and various incident waves. For the same focused incident waves, the normalized GF and SF are similar for different particle sizes, while the total force can be quite different owing to the varying relative strength between the GF and SF. GF and SF possess symmetries that are not found in the incident waves, indicating that these physically and mathematically distinct forces have symmetries that are hidden from the beam profile. For a vortex beam carrying a well-defined topological charge, acoustic forces alone cannot trap particles.

العلاقة: https://repository.hkust.edu.hk/ir/Record/1783.1-136389Test; New Journal of Physics, v. 26, (2), February 2024, article number 023019; https://doi.org/10.1088/1367-2630/ad20b1Test; http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=1367-2630&rft.volume=26&rft.issue=2&rft.date=2024&rft.spage=&rft.aulast=Cheng&rft.aufirst=&rft.atitle=Conservative+and+nonconservative+forces+for+Mie+particles+inTest+acoustic+trapping&rft.title=NEW+JOURNAL+OF+PHYSICS; http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=LinksAMR&SrcApp=PARTNER_APP&DestLinkType=FullRecord&DestApp=WOS&KeyUT=001159791200001Test; http://www.scopus.com/record/display.url?eid=2-s2.0-85185196042&origin=inwardTest

الإتاحة: https://doi.org/10.1088/1367-2630/ad20b1Test
https://repository.hkust.edu.hk/ir/Record/1783.1-136389Test
http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=1367-2630&rft.volume=26&rft.issue=2&rft.date=2024&rft.spage=&rft.aulast=Cheng&rft.aufirst=&rft.atitle=Conservative+and+nonconservative+forces+for+Mie+particles+inTest+acoustic+trapping&rft.title=NEW+JOURNAL+OF+PHYSICS
http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=LinksAMR&SrcApp=PARTNER_APP&DestLinkType=FullRecord&DestApp=WOS&KeyUT=001159791200001Test
http://www.scopus.com/record/display.url?eid=2-s2.0-85185196042&origin=inwardTest

المؤلفون: Hooi Ching Lim, Shamit Soneji, Róbert Pálmason, Stig Lenhoff, Thomas Laurell, Stefan Scheding

المصدر: Biomarker Research, Vol 9, Iss 1, Pp 1-12 (2021)

مصطلحات موضوعية: Acoustic trapping, Plasma extracellular vesicles, EVs, Allogeneic hematopoietic stem cell transplantation, Allo-HSCT, Transplantation, Therapeutics. Pharmacology, RM1-950

الوصف: Abstract Background Infection and graft-versus-host disease (GvHD) are the major causes for mortality and morbidity of allogeneic hematopoietic stem cell transplantation (allo-HSCT). Plasma-derived extracellular vesicles (EVs) contain disease-related proteins, DNAs and RNAs, and have recently been suggested as potential biomarker candidates for transplantation complications. However, EV isolation from small plasma volumes in clinical biomarker studies using conventional methods is challenging. We therefore investigated if EVs isolated by novel automated acoustic trapping could be developed as potential biomarkers for allo-HSCT complications by performing a clinical proof-of-principle study. Results Plasma samples were collected from twenty consecutive patients with high-risk/relapsed hematologic malignancies undergoing allo-HSCT before transplantation and post-transplant up to 12 weeks. EVs were isolated from small plasma sample volumes (150 μl) by an automated, acoustofluidic-based particle trapping device, which utilizes a local λ/2 ultrasonic standing wave in a borosilicate glass capillary to capture plasma EVs among pre-seeded polystyrene microbeads through sound scatter interactions. We found that EVs could be reliably isolated from all plasma samples (n = 173) and that EV numbers increased more than 2-fold in the majority of patients after transplantation. Also, sufficient quantities of RNA for downstream microRNA (miRNA) analysis were obtained from all samples and EV miRNA profiles were found to differ from whole plasma profiles. As a proof of principle, expression of platelet-specific miR-142-3p in EVs was shown to correlate with platelet count kinetics after transplantation as expected. Importantly, we identified plasma EV miRNAs that were consistently positively correlated with infection and GvHD, respectively, as well as miRNAs that were consistently negatively correlated with these complications. Conclusions This study demonstrates that acoustic enrichment of EVs in a clinical biomarker study setting is feasible and that downstream analysis of acoustically-enriched EVs presents a promising tool for biomarker development in allo-HSCT. Certainly, these findings warrant further exploration in larger studies, which will have significant implications not only for biomarker studies in transplantation but also for the broad field of EV-based biomarker discovery.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2050-7771Test

الوصول الحر: https://doaj.org/article/110883f232814aeeb3bec62d7f0d3287Test

المؤلفون: Ku, Anson, Fredsøe, Jacob, Sørensen, Karina D., Borre, Michael, Evander, Mikael, Laurell, Thomas, Lilja, Hans, Ceder, Yvonne

المصدر: Frontiers in Oncology MultiPark: Multidisciplinary research focused on Parkinson´s disease NanoLund: Centre for Nanoscience EpiHealth: Epidemiology for Health. 11

مصطلحات موضوعية: acoustic trapping, extracellular vesicles, liquid biopsy, microRNA, ncRNA, prostate cancer, Medicin och hälsovetenskap, Medicinska och farmaceutiska grundvetenskaper, Cell- och molekylärbiologi, Medical and Health Sciences, Basic Medicine, Cell and Molecular Biology, Klinisk medicin, Cancer och onkologi, Clinical Medicine, Cancer and Oncology

الوصف: Molecular profiling of extracellular vesicles (EVs) offers novel opportunities for diagnostic applications, but the current major obstacle for clinical translation is the lack of efficient, robust, and reproducible isolation methods. To bridge that gap, we developed a microfluidic, non-contact, and low-input volume compatible acoustic trapping technology for EV isolation that enabled downstream small RNA sequencing. In the current study, we have further automated the acoustic microfluidics-based EV enrichment technique that enables us to serially process 32 clinical samples per run. We utilized the system to enrich EVs from urine collected as the first morning void from 207 men referred to 10-core prostate biopsy performed the same day. Using automated acoustic trapping, we successfully enriched EVs from 199/207 samples (96%). After RNA extraction, size selection, and library preparation, a total of 173/199 samples (87%) provided sufficient materials for next-generation sequencing that generated an average of 2 × 106 reads per sample mapping to the human reference genome. The predominant RNA species identified were fragments of long RNAs such as protein coding and retained introns, whereas small RNAs such as microRNAs (miRNA) accounted for less than 1% of the reads suggesting that partially degraded long RNAs out-competed miRNAs during sequencing. We found that the expression of six miRNAs was significantly different (Padj < 0.05) in EVs isolated from patients found to have high grade prostate cancer [ISUP 2005 Grade Group (GG) 4 or higher] compared to those with GG3 or lower, including those with no evidence of prostate cancer at biopsy. These included miR-23b-3p, miR-27a-3p, and miR-27b-3p showing higher expression in patients with GG4 or high grade prostate cancer, whereas miR-1-3p, miR-10a-5p, and miR-423-3p had lower expression in the GG4 PCa cases. Cross referencing our differentially expressed miRNAs to two large prostate cancer datasets revealed that the putative tumor suppressors miR-1, miR-23b, and miR-27a are consistently deregulated in prostate cancer. Taken together, this is the first time that our automated microfluidic EV enrichment technique has been found to be capable of enriching EVs on a large scale from 900 μl of urine for small RNA sequencing in a robust and disease discriminatory manner.

الوصول الحر: https://lup.lub.lu.se/record/3e6455c0-b360-4783-9ad1-ec4c2c523a9aTest
http://dx.doi.org/10.3389/fonc.2021.631021Test