المؤلفون: Xuda Wang, Zelong Zhao, Jingwei Jiang, Rui Mi, Xiaoyan Guan, Ying Dong, Shilei Li, Zhong Chen, Shan Gao, Bai Wang, Yao Xiao, Yongjia Pan, Zunchun Zhou

المصدر: Ecotoxicology and Environmental Safety, Vol 264, Iss , Pp 115407- (2023)

مصطلحات موضوعية: Apostichopus japonicus, Gut microbiota, Temporal dynamics, Community assembly, Co-occurrence network, Nanoplastics, Environmental pollution, TD172-193.5, Environmental sciences, GE1-350

الوصف: Aquaculture provides essential food for humans, and the health of farmed species is particularly important for the aquaculture industry. Aquaculture environment could be a sink of plastic debris (PDs) due to the enclosed character and heavy use of plastics. Gut microbiota of aquaculture species could respond to the exogenous pollutants and regulate the health of hosts. Here, variations in gut microbiota of Apostichopus japonicus induced by the ingested nanoplastics (NPs) were investigated by a lab experiment. We selected a NPs concentration gradient of 100 mg/kg and 500 mg/kg to simulate microplastic pollution to A. japonicus, and the significant differences in gut microbiota composition after 21 days of NP exposure were evaluated. According to the high-throughput sequencing from time series samples, a decrease of diversity in gut microbiota of A. japonicus with dietary NPs was observed. In addition, the gut microbiota compositions of sea cucumbers with and without NPs exposure were also distinct, expressing as enrichment of Bacteroidota while reducement of Proteobacteria under NPs stresses. Combined the results of network analysis, the less complexity and stability of gut microbiota in sea cucumbers with dietary NPs were proved. Based on the neutral community model, the ingested NPs elevated the contribution of stochastic processes for the gut microbiota assembly in sea cucumbers. Our study showed that substantial variations in gut microbiota of A. japonicus under NPs stresses, and also explored the underlying mechanisms regulating these changes. This research would offer new meaningful insights into the toxicity of NPs on sea cucumbers, contributing a solid fundament to improve the health of sea cucumbers under NPs stresses.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S0147651323009119Test; https://doaj.org/toc/0147-6513Test

الوصول الحر: https://doaj.org/article/cc0c08e3873c4dc38a764b875dea10f6Test

المؤلفون: Zelong Zhao, Jingwei Jiang, Yongjia Pan, Ying Dong, Bai Wang, Shan Gao, Zhong Chen, Xiaoyan Guan, Xuda Wang, Zunchun Zhou

المصدر: mSystems, Vol 7, Iss 3 (2022)

مصطلحات موضوعية: sea cucumber, Apostichopus japonicus, body vesicular syndrome, multiomics, fatty acid metabolism disorder, gut microbiota, Microbiology, QR1-502

الوصف: ABSTRACT Apostichopus japonicus (sea cucumber) is one of the most valuable aquaculture species in China; however, different diseases can limit its economic development. Recently, a novel disease, body vesicular syndrome (BVS), was observed in A. japonicus aquaculture. Diseased animals displayed no obvious phenotypic characteristics; however, after boiling at the postharvest stage, blisters, lysis, and body ruptures appeared. In this study, a multiomics strategy incorporating analysis of the gut microbiota, the pond microbiome, and A. japonicus genotype was established to investigate BVS. Detailed analyses of differentially expressed proteins (DEPs) and metabolites suggested that changes in cell adhesion structures, caused by disordered fatty acid β-oxidation mediated by vitamin B5 deficiency, could be a putative BVS mechanism. Furthermore, intestinal dysbacteriosis due to microbiome variations in pond water was considered a potential reason for vitamin B5 deficiency. Our BVS index, based on biomarkers identified from the A. japonicus gut microbiota, was a useful tool for BVS diagnosis. Finally, vitamin B5 supplementation was successfully used to treat BVS, suggesting an association with BVS etiology. IMPORTANCE Body vesicular syndrome (BVS) is a novel disease in sea cucumber aquaculture. As no phenotypic features are visible, BVS is difficult to confirm during aquaculture and postharvest activities, until animals are boiled. Therefore, BVS could lead to severe economic losses compared with other diseases in sea cucumber aquaculture. In this study, for the first time, we systematically investigated BVS pathogenesis and proposed an effective treatment for the condition. Moreover, based on the gut microbiota, we established a noninvasive diagnostic method for BVS in sea cucumber.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2379-5077Test

الوصول الحر: https://doaj.org/article/24b91c96681147bb89be668ddabc61a5Test

المؤلفون: Rong Qiao, Rong Xiao, Zhong Chen, Jingwei Jiang, Chenghua Yuan, Shuxiang Ning, Jihong Wang, Zunchun Zhou

المصدر: Molecules, Vol 27, Iss 1, p 229 (2021)

مصطلحات موضوعية: sea cucumber, ADAMTS13-like, TSP 1, RGD, anti-tumoral activity, rAj-Tspin, Organic chemistry, QD241-441

الوصف: In recent years, sea cucumber has become a favorite healthcare food due to its characteristic prevention of cardiovascular diseases, suppression of tumors, as well as enhancement of immunity. In order to screen the anti-tumoral proteins or peptides from sea cucumber (Apostichopus japonicus), its cDNA library was analyzed, and a disintegrin-like and metalloproteinase with thrombospondin type 1 motif, member 13 (ADAMTS13)-like was found. ADAMTS13-like contains 10 thrombospondin 1 (TSP1) domains. Based on analysis of bioinformatics, the third TSP1 domain of this protein, which is further named Aj-Tspin, contains an arginine-glycine-aspartate (RGD) motif. Since our previous studies showed that the recombinant RGD-containing peptide from lampreys showed anti-tumoral activity, the third TSP1 domain of ADAMTS13-like was chosen to evaluate it’s effect on tumor proliferation and metastasis, despite the fact it shares almost no homologue with disintegrins from other species. After artificial synthesis, its cDNA sequence, Aj-Tspin, which is composed of 56 amino acids, was subcloned into a pET23b vector and expressed as a recombinant Aj-Tspin (rAj-Tspin) in a soluble form with a molecular weight of 6.976 kDa. Through affinity chromatography, rAj-Tspin was purified as a single protein. Both anti-proliferation and immunofluorescence assays showed that rAj-Tspin suppressed the proliferation of Lewis lung carcinoma (LLC) cells through apoptosis. Adhesion assay also displayed that rAj-Tspin inhibited the adhesion of LLC cells to ECM proteins, including fibronectin, laminin, vitronectin and collagen. Lastly, rAj-Tspin also suppressed the migration and invasion of LLC cells across the filter in transwells. Thus, the above indicates that rAj-Tspin might act as a potential anti-tumoral drug in the future and could also provide information on the nutritional value of sea cucumber.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1420-3049/27/1/229Test; https://doaj.org/toc/1420-3049Test

الوصول الحر: https://doaj.org/article/85dcb0072d6f487a9b6cc0618d803612Test

المؤلفون: Ying Dong, Hongjuan Sun, Zunchun Zhou, Aifu Yang, Zhong Chen, Xiaoyan Guan, Shan Gao, Bai Wang, Bei Jiang, Jingwei Jiang

المصدر: International Journal of Molecular Sciences, Vol 15, Iss 11, Pp 19472-19486 (2014)

مصطلحات موضوعية: sea cucumber (Apostichopus japonicus), differentially expressed genes, quantitative real-time polymerase chain reaction, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: The sea cucumber (Apostichopus japonicus) occupies a basal position during the evolution of deuterostomes and is also an important aquaculture species. In order to identify more immune effectors, transcriptome sequencing of A. japonicus coelomocytes in response to lipopolysaccharide (LPS) challenge was performed using the Illumina HiSeq™ 2000 platform. One hundred and seven differentially expressed genes were selected and divided into four functional categories including pathogen recognition (25 genes), reorganization of cytoskeleton (27 genes), inflammation (41 genes) and apoptosis (14 genes). They were analyzed to elucidate the mechanisms of host-pathogen interactions and downstream signaling transduction. Quantitative real-time polymerase chain reactions (qRT-PCRs) of 10 representative genes validated the accuracy and reliability of RNA sequencing results with the correlation coefficients from 0.88 to 0.98 and p-value

وصف الملف: electronic resource

العلاقة: http://www.mdpi.com/1422-0067/15/11/19472Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/6189788fb96542479309276296d2ed55Test

المؤلفون: Zunchun Zhou, Shikai Liu, Ying Dong, Shan Gao, Zhong Chen, Jingwei Jiang, Aifu Yang, Hongjuan Sun, Xiaoyan Guan, Bei Jiang, Bai Wang

المصدر: PLoS ONE, Vol 10, Iss 9, p e0138585 (2015)

مصطلحات موضوعية: Medicine, Science

الوصف: Sea urchins have long been used as research model organisms for developmental biology and evolutionary studies. Some of them are also important aquaculture species in East Asia. In this work, we report the construction of RAD-tag based high-density genetic maps by genotyping F1 interspecific hybrids derived from a crossing between a female sea urchin Strongylocentrotus nudus and a male Strongylocentrotus intermedius. With polymorphisms present in these two wild individuals, we constructed a female meiotic map containing 3,080 markers for S. nudus, and a male meiotic map for S. intermedius which contains 1,577 markers. Using the linkage maps, we were able to anchor a total of 1,591 scaffolds (495.9 Mb) accounting for 60.8% of the genome assembly of Strongylocentrotus purpuratus. A genome-wide scan resulted in the identification of one putative QTL for body size which spanned from 25.3 cM to 30.3 cM. This study showed the efficiency of RAD-Seq based high-density genetic map construction using F1 progenies for species with no prior genomic information. The genetic maps are essential for QTL mapping and are useful as framework to order and orientate contiguous scaffolds from sea urchin genome assembly. The integration of the genetic map with genome assembly would provide an unprecedented opportunity to conduct QTL analysis, comparative genomics, and population genetics studies.

وصف الملف: electronic resource

العلاقة: http://europepmc.org/articles/PMC4580576?pdf=renderTest; https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/9f0b5d3e72244d4eacb68c27ec6e61d4Test

المؤلفون: Jingwei, Jiang, Zelong, Zhao, Shan, Gao, Zhong, Chen, Yongjia, Pan, Xiaoyan, Guan, Pingzhe, Jiang, Peipei, Li, Bai, Wang, Hongjuan, Sun, Ying, Dong, Zunchun, Zhou

المصدر: Fish & Shellfish Immunology. 131:1275-1281

مصطلحات موضوعية: Environmental Chemistry, General Medicine, Aquatic Science

الوصف: The lysin motif (LysM)-containing protein is one of widespread pattern-recognition receptors in prokaryotes and eukaryotes. Numerous LysM-containing gene sequences are present in gene databases; however, few have been well characterized, especially in echinoderms. In this study, the full-length cDNA of a novel LysM-containing gene was obtained from the sea cucumber Apostichopus japonicus, named AjLysM-1, using polymerase chain reaction (PCR) combined with rapid amplification of cDNA ends. We prepared and expressed recombinant AjLysM-1 protein (rAjLysM-1) and determined its pathogen-recognition ability by enzyme-linked immunosorbent and immunofluorescence assays. We also analyzed the tissue expression pattern and response to immune challenges of AjLysM-1 using quantitative real-time reverse transcription-PCR and in situ hybridization. The AjLysM-1 protein was predicted to be an intracellular non-secreted LysM-containing protein, highly homologous to the same protein in other marine echinoderms. AjLysM-1 transcripts were highest expressed in coelomocytes and were strikingly induced by challenge with representative bacterial and fungal polysaccharides. rAjLysM-1 showed weak binding to mannan, Pseudoalteromonas nigrifaciens, and Shewanella baltica, implying that AjLysM-1 might provide inadequate defense against Gram-negative bacteria and fungi. Notably, rAjLysM-1 also interacted with tyrosine protein kinase and filamin-B, indicating that it could be involved in focal adhesion in A. japonicus. These findings improve our understanding of the functions of LysM-containing proteins in marine echinoderms.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fdc56051d1957ce99e5b002de86ce21cTest
https://doi.org/10.1016/j.fsi.2022.11.016Test

المؤلفون: Pingzhe, Jiang, Shan, Gao, Zhong, Chen, Hongjuan, Sun, Peipei, Li, Dongmei, Yue, Yongjia, Pan, Xuda, Wang, Rui, Mi, Ying, Dong, Jingwei, Jiang, Zunchun, Zhou

المصدر: Fish & Shellfish Immunology. 128:67-73

مصطلحات موضوعية: DNA, Complementary, Phosphotransferases (Phosphate Group Acceptor), Base Sequence, Hydrolases, Sea Cucumbers, Mevalonic Acid, Nucleosides, General Medicine, Protein Sorting Signals, Saponins, Aquatic Science, Immunity, Innate, Diphosphates, Stichopus, Tandem Mass Spectrometry, Animals, Environmental Chemistry, Amino Acid Sequence, Cloning, Molecular, Phylogeny, Chromatography, Liquid

الوصف: The sea cucumber Apostichopus japonicus is one of the most dominant and economically important aquaculture species in China. Saponin, which possesses notable biological and pharmacological properties, is a key determinant of the nutritional and health value of A. japonicus. In the present study, we amplified the full-length cDNA of a phosphomevalonate kinase (PMK) gene (named AjPMK) using rapid amplification of cDNA ends (RACE). Subsequently, we engineered a recombinant AjPMK (rAjPMK) protein and assessed its enzymatic activity by enzyme-linked immunosorbent assay (ELISA). Proteins that interact with rAjPMK were screened and identified via pull-down assay combined with liquid chromatography with tandem mass spectrometry (LC-MS/MS). We found that the full-length cDNA of AjPMK contained 1354 bp and an open reading frame (ORF) of 612 bp. The AjPMK protein was predicted not to contain a signal peptide but to contain a phosphonolate kinase domain seen in higher eukaryotes and a P-loop with a relatively conserved nucleoside triphosphate hydrolase domain. The molecular weight of the AjPMK protein was estimated to be 23.81 kDa, and its isoelectric point was predicted to be 8.72. Phylogenetic analysis showed that AjPMK had a closer evolutionary relationship with genes from starfish than with those of other selected species. Besides, we found that rAjPMK synthesized mevalonate-5-diphosphate, interacted either directly or indirectly with crucial pattern recognition receptors (PRRs) and was regulated by immune-related processes, including antioxidative reactions, stress resistance responses and enzyme hydrolysis. Moreover, AjPMK also interacted with farnesyl pyrophosphate synthase, an enzyme reported to be involved in saponin biosynthesis. Together, our findings implied that AjPMK may be directly involved in saponin biosynthesis and the regulation of various innate immune processes.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9fcf430d67525f7b505ecdd05ac17114Test
https://doi.org/10.1016/j.fsi.2022.07.073Test

المؤلفون: Ramesha Thimmappa, Shi Wang, Minyan Zheng, Rajesh Chandra Misra, Ancheng C. Huang, Gerhard Saalbach, Yaqing Chang, Zunchun Zhou, Veronica Hinman, Zhenmin Bao, Anne Osbourn

المصدر: Nature Chemical Biology. 18:774-781

مصطلحات موضوعية: Sterols, Glycosylation, Sea Cucumbers, Animals, Cell Biology, Saponins, Molecular Biology, Triterpenes

الوصف: Soft-bodied slow-moving sea creatures such as sea stars and sea cucumbers lack an adaptive immune system and have instead evolved the ability to make specialized protective chemicals (glycosylated steroids and triterpenes) as part of their innate immune system. This raises the intriguing question of how these biosynthetic pathways have evolved. Sea star saponins are steroidal, while those of the sea cucumber are triterpenoid. Sterol biosynthesis in animals involves cyclization of 2,3-oxidosqualene to lanosterol by the oxidosqualene cyclase (OSC) enzyme lanosterol synthase (LSS). Here we show that sea cucumbers lack LSS and instead have two divergent OSCs that produce triterpene saponins and that are likely to have evolved from an ancestral LSS by gene duplication and neofunctionalization. We further show that sea cucumbers make alternate sterols that confer protection against self-poisoning by their own saponins. Collectively, these events have enabled sea cucumbers to evolve the ability to produce saponins and saponin-resistant sterols concomitantly.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::308ce17ae156bfd9b049ff70dd58f9b5Test
https://doi.org/10.1038/s41589-022-01054-yTest

المؤلفون: Guiying Liu, Xuda Wang, Kun Ge, Jinhao Wu, Zhaohui Wang, Jing Du, Lun Song, Zunchun Zhou

المصدر: Journal of Ocean University of China. 21:473-478

مصطلحات موضوعية: Ocean Engineering, Oceanography

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::68c8f4393837a2d0b35a17ef6df673b0Test
https://doi.org/10.1007/s11802-022-4854-0Test

المؤلفون: Bailing Chen, Yulong Li, Qidou Gao, Yunfeng Li, Xiangbo Bao, Jie Zheng, Zunchun Zhou

الوصف: Rhopilema esculentum is widely cultured in Chinese coastal regions and has thousand years of consumption history in China. Despite R. esculentum owns multiple colors (mainly ochre and blue) in aquaculture ponds, little is known about the pigments and color formation. This study identified and verified the blue pigment of R. esculentum. Results showed that the blue pigment of R. esculentum has 262 amino acids with 54.96% identity to the blue pigment protein precursor of Rhizostoma pulmo. The blue pigment of R. esculentum has Kringle and Frizzled domains, which is in accordance with rhizostomins characteristics. Compared to ochre R. esculentum, the expression level of blue pigment increased 3.49-fold in blue R. esculentum. In addition, overexpression of the blue pigment in Rhodosporidium toruloides made transformant cells blue under blue light excitation. Our findings provide knowledge for the blue color formation of R. esculentum and will benefit the color selection of R. esculentum with excellent traits.

العلاقة: https://doi.org/10.1155/2023/2994722Test

الإتاحة: https://doi.org/10.1155/2023/2994722Test