المؤلفون: Xiaorui Wang, Zihan Geng, Yanyan Bao, Juying Zhong, Jing Ma, Xiaolan Cui, Yujing Shi

المصدر: Heliyon, Vol 10, Iss 12, Pp e33237- (2024)

مصطلحات موضوعية: Influenza A virus, Pneumonia, Cellular immune response, Inflammation, Cytokine, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: Background: Death caused by respiratory tract infection is one of the leading causes of death in the world today. Shufeng Jiedu Capsule (SFJDC) is a traditional Chinese medicine that has been widely used clinically for coronavirus disease 2019 (COVID-19), H1N1 influenza virus pneumonia and other diseases. Its pharmacological effect is to inhibit inflammation and improve the body's ability to clear viruses. However, the mechanism of SFJDC in the treatment of viral pneumonia, especially its effect on the inflammatory-immune microenvironment of lung tissue remains unclear. Methods: Mice with H1N1 influenza virus pneumonia were used as a model to verify the efficacy of SFJDC through death protection, lung index, viral load, and HE staining of lung tissue. The levels of inflammatory cytokines and chemokines in lung tissue were investigated by multi-analyte immunoassay. The number and proportion of cells in peripheral blood were detected by blood routine. The percentage of infiltrating immune cells in lung tissue was detected by flow cytometry and immunofluorescence. Results: SFJDC (2.2 g/kg·d−1 and 1.1 g/kg·d−1) increased survival rate (P＜0.01, P＜0.05), prolonged the survival period of mice, and alleviated the histopathological damage in lung (P＜0.01). SFJDC (2.2 g/kg·d−1, 1.1 g/kg·d−1 and 0.055 g/kg·d−1) increased body weight(P＜0.01, P＜0.05), improved activity status, reduced the lung index (P＜0.01, P＜0.05) and viral load (P＜0.01). SFJDC (2.2 g/kg·d−1 and 1.1 g/kg·d−1) reduced interleukin-1β (IL-1β), interleukin-18(IL-18), tumour necrosis factor α (TNF-α), monocyte chemoattractant protein (MCP), chemokine (C-X-C motif) ligand 1 (CXCL1) (P＜0.01, P＜0.05), and SFJDC (2.2 g/kg·d−1) increased IL-10 levels (P＜0.05) to regulate inflammation. SFJDC (2.2 g/kg·d−1) increased the percentages of CD4+ T cells (P＜0.01), CD8+ T cells (P＜0.05), and B cells(P＜0.05), and decreased F4/80+ macrophages (P＜0.05). Conclusion: Our findings indicated that SFJDC could inhibit inflammation and lung injury while maintaining the function of the adaptive immune response mediated by T and B cells, and promote the clearance of the virus, thereby treating influenza A (H1N1) virus-induced pneumonia.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844024092685Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/edc4a3cbcd6d4146848be54dd303b2d8Test

المؤلفون: Zonghao Liu, Huan Zhang, Mi Zhou, Shuming Jiao, Xiao-Ping Zhang, Zihan Geng

المصدر: IEEE Access, Vol 12, Pp 78496-78504 (2024)

مصطلحات موضوعية: Single-pixel imaging, super-resolution, generative adversarial network, computational imaging, perceptual image-error assessment, ultra-low sampling rate, Electrical engineering. Electronics. Nuclear engineering, TK1-9971

الوصف: Single-pixel imaging (SPI) leverages sequential pattern illumination and intensity detection to reconstruct images, facing the challenge of balancing high-resolution output with ultra-low sampling rates for rapid imaging processes. We introduce a network architecture specifically tailored for SPI, which demonstrates improved performance even before integrating with SPI’s physical sampling processes. This integration, particularly focusing on the nuanced effects of sampling rates within the model’s loss function and data preprocessing, enhances image reconstruction quality and adaptability at low sampling rates, down to 1.56%. Our approach achieves a balance between advanced computational methods and the physical principles of SPI, resulting in a peak signal-to-noise ratio of 30.93 dB, a structural similarity index measure of 0.8818, and a perceptual index (PI) of 5.31 at a 6.25% sampling rate, alongside a notable PI of 2.68 at a 1.56% sampling rate in practical tests. By merging sophisticated network design with strategic integration of physical sampling rates, our model provides a refined solution for high-quality, high-resolution SPI at minimal sampling rates, facilitating progress in ultra-fast imaging applications.

وصف الملف: electronic resource

العلاقة: https://ieeexplore.ieee.org/document/10534776Test/; https://doaj.org/toc/2169-3536Test

الوصول الحر: https://doaj.org/article/bb2f4bc05d144a798c895d532681b0f7Test

المؤلفون: Yuan Wang, Honglian Xu, Zihan Geng, Guiling Geng, Feng Zhang

المصدر: BMC Public Health, Vol 23, Iss 1, Pp 1-11 (2023)

مصطلحات موضوعية: Dementia, Medical history, Older adults, Community, Public aspects of medicine, RA1-1270

الوصف: Abstract Introduction Many studies have revealed the effect of medical history on dementia. The aim of this study was to explore the relationship between the history of disease and onset of dementia. Methods This was a multi-center, cross-sectional study, with 2595 older adults enrolled. The onset of dementia was evaluated with Revised Hasegawa Dementia Scale (HDS-R). The diagnosed diseases after the age of 40 of the participants were investigated, including respiratory system diseases, digestive system diseases, cardiovascular diseases, endocrine disorders, genitourinary system diseases, nervous system disease, sensory system diseases, dental/oral diseases, bone/joint diseases and mental illnesses. Results Data of 2458 older adults were analyzed. Univariate analysis showed that diabetes, thyroid disease, mental illness, hearing loss, stroke, dental/oral disease, Denture use, fracture/osteoporosis, kidney disease and number of diseases were risk factors for dementia. After controlling for demographic sociological variables, diabetes, dental/oral disease, and denture use were independent risk factors for dementia. Thyroid disease (P = 0.313), mental illnesses (P = 0.067), hearing loss (P = 0.595), stroke (P = 0.538), fractures/osteoporosis (P = 0.069), kidney disease (P = 0.168) were no longer significant to dementia. Conclusion Diabetes, dental/oral disease and denture use were main risk factors for dementia.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2458Test

الوصول الحر: https://doaj.org/article/d659f9960fb94ecb8d15b35d5bf25aceTest

المؤلفون: Yang Yao, Yingnan Liu, Yu Chang, Zihan Geng, Xingting Liu, Songnan Ma, Zhiyun Wang, Chenguang Zheng, Jiajia Yang, Dong Ming

المصدر: Frontiers in Human Neuroscience, Vol 17 (2023)

مصطلحات موضوعية: COVID-19, electroencephalography, functional connectivity network, sample entropy, directed transfer function, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571

الوصف: ObjectiveThe coronavirus disease 2019 (COVID-19) is an acute respiratory infectious disease caused by the SARA-CoV-2, characterized by high infectivity and incidence. Clinical data indicates that COVID-19 significantly damages patients’ perception, motor function, and cognitive function. However, the electrophysiological mechanism by which the disease affects the patient’s nervous system is not yet clear. Our aim is to investigate the abnormal levels of brain activity and changes in brain functional connectivity network in patients with COVID-19.MethodsWe compared and analyzed electroencephalography signal sample entropy, energy spectrum, and brain network characteristic parameters in the delta (1–4 Hz), theta (4–8 Hz), alpha (8–13 Hz), and beta (13–30 Hz) bands of 15 patients with COVID-19 and 15 healthy controls at rest.ResultsAt rest, energy values of the four frequency bands in the frontal and temporal lobes of COVID-19 patients were significantly reduced. At the same time, the sample entropy value of the delta band in COVID-19 patients was significantly increased, while the value of the beta band was significantly decreased. However, the average value of the directed transfer function of patients did not show any abnormalities under the four frequency bands. Furthermore, node degree in the temporal lobe of patients was significantly increased, while the input degree of the frontal and temporal lobes was significantly decreased, and the output degree of the frontal and occipital lobes was significantly increased.ConclusionThe level of brain activity in COVID-19 patients at rest is reduced, and the brain functional network undergoes a rearrangement. These results preliminarily demonstrate that COVID-19 patients exhibit certain brain abnormalities during rest, it is feasible to explore the neurophysiological mechanism of COVID-19’s impact on the nervous system by using EEG signals, which can provide a certain technical basis for the subsequent diagnosis and evaluation of COVID-19 using artificial intelligence and the prevention of brain nervous system diseases after COVID-19 infection.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fnhum.2023.1280362/fullTest; https://doaj.org/toc/1662-5161Test

الوصول الحر: https://doaj.org/article/7b0e6f35d6d541eaa75a6194413b2848Test

المؤلفون: Lipeng Li, Zijie Pei, Ruiting Wu, Yaling Zhang, Yaxian Pang, Huaifang Hu, Wentao Hu, Zihan Geng, Tengfei Feng, Yujie Niu, Guimin Hao, Rong Zhang

المصدر: Ecotoxicology and Environmental Safety, Vol 263, Iss , Pp 115309- (2023)

مصطلحات موضوعية: PM2.5, Leydig cell, Ferroptosis, FDX1, Testosterone, Sperm, Environmental pollution, TD172-193.5, Environmental sciences, GE1-350

الوصف: Epidemiological studies have established an association between chronic exposure to PM2.5 and male infertility. However, the underlying mechanisms were not fully revealed. In this study, we established mice models exposed to PM2.5 for 16 weeks, and a significant decrease in sperm quality accompanied by an increase in testosterone levels were observed after PM2.5 exposure. Moreover, treatment with ferrostatin-1 (Fer-1), a specific ferroptosis inhibitor, effectively mitigated PM2.5-induced testicular dysfunction in mice. And lipid peroxidation and ferritin accumulation were found to be significantly increased in Leydig cells of testes with a PM2.5-dose dependent manner. Further investigations revealed that TM-3 cells, a mouse Leydig cell line, were prone to ferroptosis after PM2.5 exposure, and the cell viability was partly rescued after the intervention of Fer-1. Furthermore, our results supported that the ferroptosis of TM-3 cells was attributed to the upregulation of ferredoxin 1 (FDX1), which was the protein transferring electrons to cytochrome P450 family 11 subfamily A member 1 to aid lysing cholesterol to pregnenolone at initial of steroidogenesis. Mechanically, PM2.5-induced FDX1 upregulation resulted in cellular ROS elevation and ferrous iron overload, which together initiated an autoxidation process of polyunsaturated fatty acids in the cell membrane of Leydig cells until the accumulated lipid peroxides triggered ferroptotic cell death. Simultaneously, upregulation of FDX1 promoted steroidogenesis and let to an increased level of testosterone. In summary, our work suggested that FDX1, a mediator involving steroidogenesis, was a key regulator in PM2.5-induced Leydig cells ferroptosis.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S0147651323008138Test; https://doaj.org/toc/0147-6513Test

الوصول الحر: https://doaj.org/article/a77f3703d2e34f32a7b3f887c5c8af8aTest

المؤلفون: Bo Pang, Ronghua Zhao, Bo Peng, Lei Bao, Zihan Geng, Shuran Li, Yingli Xu, Lirun Zhou, Shanshan Guo, Xiaolan Cui, Jing Sun

المصدر: Heliyon, Vol 9, Iss 7, Pp e17802- (2023)

مصطلحات موضوعية: Traditional Chinese medicine, β-hemolytic Streptococcus, Antibacterial, Antiviral, TMT quantitative proteomic, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: Context: Kaihoujian Throat Spray children's type (KHJSC) is a Chinese medicine prescription for treating pediatric acute pharyngitis and tonsillitis (APT). However, its relevant mechanisms remain unclear. Objective: To investigate the pharmacological effects of KHJSC on APT in vitro and in vivo, and explore the possible mechanism and target proteins. Materials and methods: The antiviral and antibacterial effects in vitro were evaluated by IC50 and MICs. Thirty-six Japanese white rabbits were averagely divided into control group, model group, amoxicillin group and 3 dose groups of KHJSC (720, 540 and 360 μL/kg/d). The model rabbits were injected with β-hemolytic Streptococcus solution into the tonsils for 2 consecutive days. KHJSC treatment started on the third day. The whole blood, serum, tonsil tissues and pharyngeal mucosa tissues were collected for routine blood tests, proteomic, ELISA and other tests on the sixth day. Results: The IC50 of KHJSC on HCoV-229E, influenza PR8 and Ad3 were 1.99, 1.99 and 4.49 mg/mL, respectively; MICs of MDR-PA, MRSA and β-hemolytic Streptococcus were 350, 350, and 175 mg/mL. KHJSC markedly decreased the number of white blood cells, lymphocytes, neutrophils, and the level of IL-1β, IL-5, IL-6, IL-18, TNF-α and MCP-1; increased the content of IL-2 and IFN-γ. Proteomic analysis and ELISA revealed that PI3K-Akt signaling pathway, NF-κB signaling pathway and Toll-like receptor signaling pathway were the potential mechanisms of KHJSC against APT. Discussion and conclusion: These results provided the reference and scientific basis for the application of KHJSC in clinic and further mechanisms study.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844023050107Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/16a24402909a48819e146d4fc3d7e369Test

المؤلفون: Meijun Pang, Xin Song, Yaodong Miao, Yiwen Wang, Chang Zhou, Zihan Geng, Jiayin Du, Bernard Moussian, Yanfang Su, Xiuyun Liu, Dong Ming

المصدر: BMEMat, Vol 1, Iss 2, Pp n/a-n/a (2023)

مصطلحات موضوعية: Aconitum Carmichaelii, apoptosis, neurotoxicity, oxidative stress, zebrafish, Biotechnology, TP248.13-248.65, Materials of engineering and construction. Mechanics of materials, TA401-492

الوصف: Abstract Background Aconitum carmichaelii, the mother root of Aconitum, has a long‐applied history for treating many diseases in China. Increased use of it has prompted significant concerns regarding its extensive cardiotoxicity and neurotoxicity. The molecular mechanisms underlying A. carmichaelii‐induced neurotoxicity are poorly understood. Methods We took advantage of the zebrafish model to investigate the neurotoxic mechanism of A. carmichaelii. In addition to the behavior and neuronal activity testing, gene expression of neuronal oxidative stress, and apoptosis levels were also analyzed. Results In contrast to the excitatory effect of low‐dose A. carmichaelii decoction, hypoactivity of locomotor behavior, and neural activity, especially telencephalon, were detected in the 20 mg/mL group. High doses of A. carmichaelii induced excessive ROS by downregulating DJ‐1 and activating Nox5, and further triggered cell apoptosis through the bax/bcl2a‐caspase‐9 pathway in zebrafish larva. Mitochondrial protection‐related genes PinK1 and Parkin were upregulated to protect against mitochondrial dysfunction during cellular stress. The ROS scavenger‐NAC significantly alleviated neurotoxicity in the 20 mg/mL group. Conclusion The study reveals the potential mechanism of A. carmichaelii‐induced neurotoxicity and provides new insights into a significant risk marker of A. carmichaelii poisoning.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2751-7446Test

الوصول الحر: https://doaj.org/article/d7335791880c42cf801403aca45f6036Test

المؤلفون: Fengbo Yang, Jianing Hua, Guiling Geng, Min Cui, Wenwen Yang, Zihan Geng

المصدر: BMC Geriatrics, Vol 22, Iss 1, Pp 1-11 (2022)

مصطلحات موضوعية: Transitional care, Instrumental support, Older adult, Chronic disease, Questionnaire, Geriatrics, RC952-954.6

الوصف: Abstract Background Previous studies indicated that poor quantity and quality of instrumental support are one of the main barriers in the application of transitional care. Instrumental support, as one common function of social support, is the provision of financial assistance, material goods, or services. The purpose of our study is to develop an Instrumental Support in Transitional Care Questionnaire (ISTCQ) and use this questionnaire to make an assessment among older adults with chronic diseases. Methods The draft questionnaire was examined by 18 experts from different professional fields performing three rounds of content validity testing with the Delphi method. Afterward, we conducted a pilot test recruiting 174 participants as a convenience sample in Nantong, China. The construct validity was confirmed via exploratory factor analysis and reliability was assessed using Cronbach's alpha. Results The authority coefficient of experts was 0.74–0.99 and Kendall harmony coefficient W was 0.381. The exploratory factor analysis indicated that the questionnaire can be interpreted by three factors: namely, anticipated support (items 1, 2, 3, 4), received support (items 5, 6, 7, 8) and support satisfaction (items 9, 10, 11, 12). These three factors (eigenvalues > 1 and factor loading > 0.4) explained 69.128% of the total variance. Furthermore, the calculation of Cronbach's alpha and test–retest reliability have shown good reliability among each dimension of the 12-item questionnaire (Cronbach's alpha 0.711–0.827, test–retest reliability 0.704–0.818). Conclusion Results from the pilot test demonstrated excellent reliability and validity of ISTCQ through each dimension and as an entire.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2318Test

الوصول الحر: https://doaj.org/article/0a7d2f3010b849c886b06a279e3b9e60Test

المؤلفون: Min Cui, Jianing Hua, Xiaoliu Shi, Wenwen Yang, Zihan Geng, Xiangyun Qian, Guiling Geng

المصدر: BMC Nursing, Vol 21, Iss 1, Pp 1-9 (2022)

مصطلحات موضوعية: Instrumental support, Older adults, Transitional care, Nursing, RT1-120

الوصف: Abstract Background Instrumental support, which is defined as practical, tangible, and informational assistance extended to patients, is crucial for older people in transition. However, little is known about instrumental support in transitional care. Thus, the aim of this study was to evaluate the instrumental support of older people in transitional care. Methods This cross-sectional study was conducted using the Questionnaire of Instrumental Support in Transitional Care (QISCT) to collect data from 747 older people in China from September to November 2020. Survey items consisted of a sociodemographic characteristics questionnaire and the QISCT. Multiple regression analyses were conducted to examine the association between independent variables and the QISCT scores. Results The total score of the QISCT was 39.43 (± 9.11), and there was a significant gap between the anticipated support and received support. The satisfaction of instrumental support was low. Multiple regression analyses showed that educational level, the number of intimate relationships, monthly family income, monthly costs of transitional care, diabetes, and chronic obstructive pulmonary disease were associated with instrumental support in transitional care. Conclusions To cope with the burden caused by chronic disease, the government and transitional care teams should establish a demand-oriented transitional care service model and pay more attention to helping older people obtain adequate and satisfactory instrumental support.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1472-6955Test

الوصول الحر: https://doaj.org/article/a29f04a8b9a549dfb22afb263e8e5d5dTest

المؤلفون: Wei Su, Hao Hu, Qiurong Ding, Min Wang, Yan Zhu, Zhaochao Zhang, Zihan Geng, Shengli Lin, Pinghong Zhou

المصدر: Cell Communication and Signaling, Vol 20, Iss 1, Pp 1-12 (2022)

مصطلحات موضوعية: NEK2, Esophageal squamous cell carcinoma, YAP1, Epithelial-mesenchymal transition, Phosphorylation, Medicine, Cytology, QH573-671

الوصف: Abstract Background Esophageal Squamous Cell Carcinoma (ESCC) was characterized as a regional-prevalent and aggressive tumor with high morbidity and mortality. NIMA-related kinase 2 (NEK2) is an interesting oncogene, the alteration of which leads to patients-beneficial outcomes. We aimed to explore the role of NEK2 in ESCC and excavate its mechanism. Methods RNA-seq data were downloaded from TCGA and GEO and analyzed by R software. The protein levels were detected by immunohistochemistry (IHC) or western blot (WB), and mRNA expression was detected by qRT-PCR. The in vitro role of proliferation and migration was detected by Transwell migration assay and by colony formation assay, respectively. The in vivo roles were explored using a subcutaneous xenograft tumor model, where immunofluorescence (IF) and IHC were employed to investigate expression and localization. The interaction between proteins was detected by immunoprecipitation. The stability of proteins was measured by WB in the presence of cycloheximide. Results A higher level of NEK2 was found in ESCC than normal esophageal epithelia in GEO, TCGA, and tissue microarray, which was associated with worse prognoses. The NEK2 knockdown impaired the proliferation and migration of ESCC, which also downregulated YAP1 and EMT markers like N-cadherin and Vimentin in vitro. On the contrary, NEK2 overexpression enhanced the migration of ESCC and elevated the levels of YAP1, N-cadherin, and Vimentin. Additionally, the overexpression of YAP1 in NEK2 knocked down ESCCs partly rescued the corresponding decrease in migration. The knockdown of NEK2 played an anti-tumor role in vivo and was accompanied by a lower level and nucleus shuffling of YAP1. In mechanism, NEK2 interacted with YAP1 and increased the stability of both endogenous and exogenous YAP1 by preventing ubiquitination. Moreover, the computer-predicted phosphorylation site of YAP1, Thr-143, reduced the ubiquitination of HA-YAP1, strengthened its stability, and thus influenced the migration in vitro. Conclusions NEK2 is a prognostic oncogene highly expressed in ESCC and promotes the progression of ESCC in vitro and in vivo. Mechanistically, NEK2-mediated phosphorylation of YAP1 at Thr-143 protects it from proteasome degradation and might serve as a promising therapeutic target in ESCC. Video Abstract

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1478-811XTest

الوصول الحر: https://doaj.org/article/c5d39095891842c6b86cdc458857f42cTest