المؤلفون: Zhou, Guang-Quan, Hua, Shi-Hao, He, Yikang, Wang, Kai-Ni, Zhou, Dandan, Wang, Hongxing, Wang, Ruoli

المصدر: IEEE transactions on neural systems and rehabilitation engineering. 31:851-862

مصطلحات موضوعية: Ultrasonic imaging, Junctions, Tendons, Muscles, Ultrasonic variables measurement, Speckle, Phase measurement, Myotendinous junction detection, ultrasound, hierarchical clustering, Hessian matrix, phase congruency, Gaussian templates

الوصف: Tracking the myotendinous junction (MTJ) motion in consecutive ultrasound images is essential to assess muscle and tendon interaction and understand the mechanics' muscle-tendon unit and its pathological conditions during motion. However, the inherent speckle noises and ambiguous boundaries deter the reliable identification of MTJ, thus restricting their usage in human motion analysis. This study advances a fully automatic displacement measurement method for MTJ using prior shape knowledge on the Y-shape MTJ, precluding the influence of irregular and complicated hyperechoic structures in muscular ultrasound images. Our proposed method first adopts the junction candidate points using a combined measure of Hessian matrix and phase congruency, followed by a hierarchical clustering technique to refine the candidates approximating the position of the MTJ. Then, based on the prior knowledge of Y-shape MTJ, we finally identify the best matching junction points according to intensity distributions and directions of their branches using multiscale Gaussian templates and a Kalman filter. We evaluated our proposed method using the ultrasound scans of the gastrocnemius from 8 young, healthy volunteers. Our results present more consistent with the manual method in the MTJ tracking method than existing optical flow tracking methods, suggesting its potential in facilitating muscle and tendon function examinations with in vivo ultrasound imaging.

وصف الملف: print

الوصول الحر: https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-325255Test
https://doi.org/10.1109/TNSRE.2023.3235587Test

المؤلفون: Zhou, Dandan (ORCID 0000-0002-2132-1044), Hedges, Helen (ORCID 0000-0003-2436-9307)

المصدر: International Journal of Early Years Education. 2023 31(3):661-674.

تمت مراجعته من قبل الزملاء: Y

Page Count: 14

الواصفات: Foreign Countries, Multiple Intelligences, Kindergarten, Preschool Teachers, Influences, Teaching Methods, Teacher Education, Educational Change

مصطلحات جغرافية: China

مستخلص: Education reform in early childhood education in China has often adopted western theories and pedagogies in efforts to facilitate educational change. The theory of multiple intelligences (MI) is an example of one adopted. This paper reports on a study that examined factors that influence Chinese kindergarten teachers' use of MI theory in their pedagogy. The study was a qualitative case study with seven participants from two kindergartens. Multiple methods were used to understand the contexts of these teachers' work, their understandings and practices, and ways that they had become exposed to ideas about MI. A number of influences were apparent in shaping teacher understandings and practices. These influences at times motivated teachers to incorporate MI, but there were also instances where teachers were unsure and confused about MI and struggled to meld it with their existing beliefs and practices based on longstanding Chinese values and pedagogies. The paper argues that if the policy goal of any educational reform is to facilitate teacher change then the introduction of new educational theories or approaches should first be grounded in, and connected carefully with, teachers' practices and, second, take careful consideration of the context in which their teaching will be embedded.

Abstractor: As Provided

المؤلفون: Fu, Yujie, Lei, Chun, Teng, Long, Zhu, Yongbing, Jiang, Liyao, Cai, Yuqin, Zhou, Dandan, Tao, Zhi

المصدر: IEEE Photonics Journal ; page 1-7 ; ISSN 1943-0655 1943-0647

الإتاحة: https://doi.org/10.1109/jphot.2024.3412201Test
http://xplorestaging.ieee.org/ielx8/4563994/4814557/10553235.pdf?arnumber=10553235Test

المؤلفون: Zhu, Baohua, Jiang, Jie, Yu, Hui, Huang, Lan, Zhou, Dandan

المساهمون: Nanjing Medical Science and Technology Development Program

المصدر: Scientific Reports ; volume 14, issue 1 ; ISSN 2045-2322

مصطلحات موضوعية: Multidisciplinary

الوصف: Our study focused on evaluating the effect of three common vasoactive drugs on the prognosis of elderly patients with sepsis and pre-existing heart failure. The Medical Information Mart for Intensive Care III database, Version 1.4, was used. Our study included critically ill older patients (aged ≥ 65 years) with sepsis and heart failure treated with vasoactive drugs. Patients were divided into norepinephrine group, norepinephrine combined with vasopressin group, and dopamine group. The baseline characteristics, primary outcome, and secondary outcome measures were compared among the three groups. In total, 1357 elderly patients were included (766 in norepinephrine group, 250 in norepinephrine combined with vasopressin group, and 341 in dopamine group). After propensity score matching, statistically significant differences in 28-d and 90-d mortality ( P = 0.046, P = 0.031) were observed; meanwhile, there was a significant difference in the incidence of mechanical ventilation, AKI, and malignant arrhythmias. Cox regression analysis revealed that norepinephrine combined with vasopressin decreased 5-year survival statistically( P = 0.001). Multiple linear regression analysis indicated dopamine as an independent risk factor in reducing ICU and hospital length of stay ( P = 0.001, P = 0.017). Logistic regression analysis showed dopamine was an independent risk factor for new-onset arrhythmias ( P < 0.001), while norepinephrine combined with vasopressin was an independent risk factor for new-onset malignant arrhythmias ( P < 0.001). Norepinephrine in combination with vasopressin decreased survival and increased the incidence of malignant arrhythmias in elderly sepsis patients with pre-existing heart failure. Dopamine alone reduces ICU and hospital length of stay but increases the new-onset arrhythmias.

الإتاحة: https://doi.org/10.1038/s41598-024-52514-5Test
https://www.nature.com/articles/s41598-024-52514-5.pdfTest
https://www.nature.com/articles/s41598-024-52514-5Test

المؤلفون: Dong, Yuchen, Zhou, Dandan, Zhang, Binzhe, Xu, Xiaoying, Zhang, Jian

المصدر: Frontiers in Cellular and Infection Microbiology ; volume 14 ; ISSN 2235-2988

مصطلحات موضوعية: Infectious Diseases, Microbiology (medical), Immunology, Microbiology

الوصف: Edwardsiella piscicida , a significant intracellular pathogen, is widely distributed in aquatic environments and causes systemic infection in various species. Therefore, it’s essential to develop a rapid, uncomplicated and sensitive method for detection of E. piscicida in order to control the transmission of this pathogen effectively. The recombinase-aided amplification (RAA) assay is a newly developed, rapid detection method that has been utilized for various pathogens. In the present study, a real-time RAA (RT-RAA) assay, targeting the conserved positions of the EvpP gene, was successfully established for the detection of E. piscicida . This assay can be performed in a one-step single tube reaction at a temperature of 39°C within 20 min. The RT-RAA assay exhibited a sensitivity of 42 copies per reaction at a 95% probability, which was comparable to the sensitivity of real-time quantitative PCR (qPCR) assay. The specificity assay confirmed that the RT-RAA assay specifically targeted E. piscicida without any cross-reactivity with other important marine bacterial pathogens. Moreover, when clinical specimens were utilized, a perfect agreement of 100% was achieved between the RT-RAA and qPCR assays, resulting a kappa value of 1. These findings indicated that the established RT-RAA assay provided a viable alternative for the rapid, sensitive, and specific detection of E. piscicida .

الإتاحة: https://doi.org/10.3389/fcimb.2024.1355056Test

المؤلفون: Gu, Wanjun, Shi, Junchao, Liu, Hui, Zhang, Xudong, Zhou, Jin J, Li, Musheng, Zhou, Dandan, Li, Rui, Lv, Jingzhu, Wen, Guoxia, Zhu, Shanshan, Qi, Ting, Li, Wei, Wang, Xiaojing, Wang, Zhaohua, Zhu, Hua, Zhou, Changcheng, Knox, Kenneth S, Wang, Ting, Chen, Qi, Qian, Zhongqing, Zhou, Tong

المصدر: Molecular Cancer. 19(1)

مصطلحات موضوعية: Biochemistry and Cell Biology, Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Biological Sciences, Rare Diseases, Lung, Genetics, Clinical Research, Cancer, Prevention, Tuberculosis, Infectious Diseases, Lung Cancer, Biotechnology, 4.2 Evaluation of markers and technologies, Detection, screening and diagnosis, 4.1 Discovery and preclinical testing of markers and technologies, Good Health and Well Being, Biomarkers, Tumor, Case-Control Studies, Cohort Studies, Gene Expression Regulation, Neoplastic, Humans, Leukocytes, Mononuclear, Lung Neoplasms, Prognosis, RNA, Small Untranslated, Lung cancer, tsRNA, rsRNA, ysRNA, Oncology & Carcinogenesis, Biochemistry and cell biology, Oncology and carcinogenesis

الوصف: One unmet challenge in lung cancer diagnosis is to accurately differentiate lung cancer from other lung diseases with similar clinical symptoms and radiological features, such as pulmonary tuberculosis (TB). To identify reliable biomarkers for lung cancer screening, we leverage the recently discovered non-canonical small non-coding RNAs (i.e., tRNA-derived small RNAs [tsRNAs], rRNA-derived small RNAs [rsRNAs], and YRNA-derived small RNAs [ysRNAs]) in human peripheral blood mononuclear cells and develop a molecular signature composed of distinct ts/rs/ysRNAs (TRY-RNA). Our TRY-RNA signature precisely discriminates between control, lung cancer, and pulmonary TB subjects in both the discovery and validation cohorts and outperforms microRNA-based biomarkers, which bears the diagnostic potential for lung cancer screening.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/0fz218m0Test

المؤلفون: He, Xiang (ORCID 0000-0002-9086-312X), Zhou, Dandan, Zhang, Xiaofei

المصدر: SAGE Open. Jul-Sep 2021 11(3):2021.

تمت مراجعته من قبل الزملاء: Y

Page Count: 14

الواصفات: Foreign Countries, English (Second Language), Second Language Instruction, College Students, Anxiety, Gender Differences, Self Efficacy, Learning Activities, Feedback (Response), Reading, Speech Communication, Translation, Reading Comprehension

مصطلحات جغرافية: China

معرفات التقييم و الدراسة: State Trait Anxiety Inventory, Foreign Language Classroom Anxiety Scale

مستخلص: Foreign language classroom anxiety (FLCA) is a very critical affective factor for learners' language learning and development. The present study was designed to explore the features of Chinese university students' FLCA and to investigate the possible influencing factors on the fluctuations of Chinese university students' FLCA. Two female and two male Chinese university students were selected as the participants and their in-class performances were recorded. Data collection instrument included the classroom observation, the self-rated evaluation of FLCA, and the stimulated recall interview. To capture the micro-changes of the Chinese learners' FLCA per second, an idiodynamic approach was adopted to carry out the exploratory analysis for the learners' experiences of FLCA over the language instruction. Zooming in on the microdynamic variation of the participants' FLCA, the final results indicated that Chinese university students' FLCA, both within individuals and across individuals, is characterized of a dynamic system's features. Furthermore, a series of contributing factors were identified to trigger the participants' FLCA, with the classroom activity types and the teacher's feedback being the key external factors and the gender difference and self-efficacy being the major internal factors. The idiodynamic method shed new light on exploring the FLCA from an emic and dynamic perspective and some pedagogical implications were also put forward as well.

Abstractor: As Provided

المؤلفون: Wang, Xun, Zhou, Dandan, Kong, Yu, Cheng, Nan, Gao, Ming, Zhang, Guqing, Ma, Junli, Chen, Yueqin, Ge, Shuang

المساهمون: Jining City of Science and Technology Bureau Key research and development project

المصدر: EJNMMI Research ; volume 13, issue 1 ; ISSN 2191-219X

مصطلحات موضوعية: Radiology, Nuclear Medicine and imaging

الوصف: Background Vertebral compression fractures (VCFs) are common clinical problems that arise from various reasons. The differential diagnosis of benign and malignant VCFs is challenging. This study was designed to develop and validate a radiomics model to predict benign and malignant VCFs with 18 F-fluorodeoxyglucose-positron emission tomography/computed tomography ( 18 F-FDG-PET/CT). Results Twenty-six features (9 PET features and 17 CT features) and eight clinical variables (age, SUVmax, SUVpeak, SULmax, SULpeak, osteolytic destruction, fracture line, and appendices/posterior vertebrae involvement) were ultimately selected. The area under the curve (AUCs) of the radiomics and clinical–radiomics models were significantly different from that of the clinical model in both the training group (0.986, 0.987 vs. 0.884, p < 0.05) and test group (0.962, 0.948 vs. 0.858, p < 0.05), while there was no significant difference between the radiomics model and clinical–radiomics model ( p > 0.05). The accuracies of the radiomics and clinical–radiomics models were 94.0% and 95.0% in the training group and 93.2% and 93.2% in the test group, respectively. The three models all showed good calibration (Hosmer–Lemeshow test, p > 0.05). According to the decision curve analysis (DCA), the radiomics model and clinical–radiomics model exhibited higher overall net benefit than the clinical model. Conclusions The PET/CT-based radiomics and clinical–radiomics models showed good performance in distinguishing between malignant and benign VCFs. The radiomics method may be valuable for treatment decision-making.

الإتاحة: https://doi.org/10.1186/s13550-023-01038-6Test

المؤلفون: Zhou, Dandan, Yuan, Zuowei, Shu, Xiaoqin, Tang, Hejun, Li, Jie, Ye, Yangmin, Tao, Nana, Zhou, Fangzhu, Zhang, Jun, Zheng, Jian, Wu, Qian, Zhang, Juan

المساهمون: MOST | National Natural Science Foundation of China, Chongqing Municipal Science and Technology Bureau

المصدر: Bioscience Reports ; volume 43, issue 11 ; ISSN 0144-8463 1573-4935

مصطلحات موضوعية: Cell Biology, Molecular Biology, Biochemistry, Biophysics

الوصف: Objectives: Alanine aminotransferase (ALT) expression is highly elevated in the serum of patients with hepatocellular carcinoma. However, the role of ALT isoenzymes in the total ALT activity remains unclear. In the present study, we systematically investigated the role of ALT isoenzymes in alcoholic and non-alcoholic fatty liver cancer. Materials and methods: The expression of ALT1 and ALT2 at the mRNA and protein levels in 25 paired primary liver cancer tissues was detected by reverse transcription quantitative PCR (RT-qPCR), Western blotting, and immunohistochemistry. Serum ALT activity was determined using an automated biochemical analyzer. Results: The mRNA and protein expression levels of ALT1 and ALT2 were lower in the tissues of alcoholic and non-alcoholic fatty liver cancers than in the paracancerous tissues. Notably, ALT2 was highly expressed in non-alcoholic fatty liver cancer tissues compared with alcoholic fatty liver cancer tissues. Total serum ALT activity was mainly contributed by ALT1 in alcoholic fatty liver cancer, whereas ALT1 contributed only marginally more to the total ALT activity than ALT2 in non-alcoholic fatty liver cancer. ALT2/ALT1 ratio can well discriminate normal control group, alcoholic liver cancer and non-alcoholic liver cancer. Conclusion: ALT1 contributed more to the total ALT activity than ALT2 in both alcoholic and non-alcoholic fatty liver cancer. Serum ALT2 to ALT activity was higher in non-alcoholic fatty liver cancer than that in alcoholic fatty liver cancer. ALT2/ALT1 ratio has some diagnostic significance for alcoholic and non-alcoholic liver cancer.

الإتاحة: https://doi.org/10.1042/bsr20230222Test