المؤلفون: Ferretti J., Minardi R., Cristiani L., Villano M., Zeppilli M.

المساهمون: Ferretti, J., Minardi, R., Cristiani, L., Villano, M., Zeppilli, M.

مصطلحات موضوعية: bioelectrosynthesi, acetogenesi, CO2, utilization

الوصف: The continuous accumulation of atmospheric CO2 requires the development of new technologies for its mitigation. Carbon capture and utilization (CCU) technologies aim to convert CO2 into precious compounds like chemicals and fuels. Biological fixation is an attractive CCU strategy in terms of cost, sustainability and variety of products. Chemoautotrophic microorganisms such as methanogens and acetogens are able to reduce CO2 into acetate and methane, respectively. Acetogens bacteria are able to use CO2 for cell growth through the Wood Liujhundal pathway, moreover, the final precursor (i.e. Acetyl-CoA) of the autotrophic metabolism, is also used in energy metabolism with acetate production as a waste product. Furthermore, it is possible to obtain multicarbon products of autotrophic origin starting from acetyl-CoA and acetate. The biotechnological use of these microorganisms requires the presence of H2 as substrate, which is used as an electron donor in the pathway. This reaction can be sustained by a biocathode in a microbial electrosynthesis cell, in which the reducing power is generated by a polarized electrode. This study proposes the use of a microbial electrosynthesis cell for conversion to acetate in H-cells by either a mixed culture enriched with Acetobacterium woodii or a pure culture of Acetobacterium woodii, to observe the difference in terms of acetate production and reducing power consumption efficiency. The mixed culture was obtained from a mixture of activated sludge and anaerobic digestate, treated by a protocol capable to select acetogenic microorganisms without the use of specific chemical inhibitors (2-Bromoethanesulfonate). Both inoculums were tested at room temperature (25°C) in the cathodic chamber of the H-cell at potentials in the range of -0.7 to -1.1 V vs SHE. The obtained results showed that the enriched mixed culture produced at -0.7 vs SHE a mixture of volatile fatty acids including C4 and C5 molecules with an overall coulombic efficiency of 50%, while at the potential of -0.9 vs SHE ...

العلاقة: volume:99; firstpage:307; lastpage:312; numberofpages:6; journal:CHEMICAL ENGINEERING TRANSACTIONS; https://hdl.handle.net/11573/1685962Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85163711125

الإتاحة: https://doi.org/10.3303/CET2399052Test
https://hdl.handle.net/11573/1685962Test

المؤلفون: Zeppilli M., Cristiani L., Ferretti J., Majone M., Villano M.

المساهمون: Zeppilli, M., Cristiani, L., Ferretti, J., Majone, M., Villano, M.

مصطلحات موضوعية: acetate production, CO2 fixation, acetogenic inoculum

الوصف: Innovative treatment and utilization of waste biomass streams are crucial for increase environmental sustainability of human activities. Sewage sludge from the biological degradation of biomass can be valorized for the selection of biocatalysts capable to convert CO2 into valuable products. Indeed, chemoautotrophic microorganisms, like methanogens and acetogens, respectively, are able to convert CO2 into CH4 or acetate by using hydrogen as electron donor, i.e., their utilization for several bio-based CO2 reutilization processes has been widely proposed by several authors. Chemoautothrophic acetogens are widely present in waste streams deriving from the organic matter degradation, however, due to the syntrophic relationship between acetogens and acetoclastic methanogens in anaerobic environments, autothropihc acetate results immediately converted into methane. Therefore, the selection of an acetogenic inoculum which allow to obtain CO2 reduction into acetate, requires methanogens inhibition. Among the different methanogen’s inhibition strategies, the most common method is the use of BES (bromo-ethane sulphonate) which results a not scalable technique for large scale application. A most promising and sustainable approach is offered by the adoption of a thermal treatment which allows to the selection of an acetogenic inoculum, thanks ot the sporogenous capacity of acetogenic bacteria. This work presents the results obtained in the thermal pre-treatment of different type of waste biomasses coming from pilot and full-scale biological processes for the selection of an acetogenic inoculum able to convert CO2 into acetate. Each waste biomass was treated by a thermal shock procedure that consisted in the treatment of the dried biomass at 120°C for 2 hours. Acetogenic inoculums obtained by the thermal pre-treatment of an acidogenic fermentate, an activated sludge and a mesophilic anaerobic digestate, were tested under hydrogenophilic conditions in comparison with blank tests and raw inoculums. The results clearly indicate ...

العلاقة: volume:92; firstpage:13; lastpage:18; numberofpages:6; journal:CHEMICAL ENGINEERING TRANSACTIONS; https://hdl.handle.net/11573/1666124Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85133932521

الإتاحة: https://doi.org/10.3303/CET2292003Test
https://hdl.handle.net/11573/1666124Test

المؤلفون: Dell'Armi E., Rossi M. M., Zeppilli M., Majone M., Petrangeli Papini M.

المساهمون: Dell'Armi, E., Rossi, M. M., Zeppilli, M., Majone, M., Petrangeli Papini, M.

مصطلحات موضوعية: reductive dechlorination, oxidative dechlorination, bioelectroremediation, chlorinated ethylenes

الوصف: The widespread contamination of chlorinated aliphatic hydrocarbons (CAHs) as Perchloroethylene (PCE) and Trichloroethylene (TCE) over the past recent years and their uncorrected disposal and storing brought these substances to become one of the most common contaminants of subsoils and groundwater in the world. In recent years, more sustainable remediation and cost-effective technologies involving groundwater’s indigenous microorganism such as the dehalorespiring microorganisms. Dehalorespiring microorganisms can reduce PCE and TCE via reductive dechlorination (RD) while aerobic dechlorinating microorganisms oxidized low chlorinated compound such as cis-dichloroethylene (cDCE) and vinyl chloride (VC) into non harmful products. The integration of reductive dechlorination and aerobic dechlorination results in an efficient approach for the complete mineralization of high chlorinated compounds, which usually led to a build-up of VC. Bioelectrochemical systems, which exploit the capability of microorganisms to interact with a polarized electrode, provide an effective strategy to promote reductive and oxidative environments by the regulation of the applied potentials. Indeed, the complete mineralization of high chlorinated CAHs, can be obtained by a sequential reductive/oxidative bioelectrochemical process which allows for the optimization of the reductive and oxidative dechlorinating conditions. In this study the performances of the reductive reactor, devoted to the reductive dechlorination has been presented with three different contaminated feeding solutions. The three different feeding solutions included an optimized mineral medium, a synthetic groundwater (constituted by tap water added with nitrate and sulphate) and a real contaminated groundwater. Moreover, different operating conditions like hydraulic retention time (HRT) and applied cathodic potential have been investigated to assess the performance of the reductive dechlorination and on side reactions. The analysis of the coulombic efficiencies for the ...

العلاقة: volume:93; firstpage:217; lastpage:222; numberofpages:6; journal:CHEMICAL ENGINEERING TRANSACTIONS; info:eu-repo/grantAgreement/EC/H2020/ELECTRA 826244; https://hdl.handle.net/11573/1666122Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85134404414

الإتاحة: https://doi.org/10.3303/CET2293037Test
https://hdl.handle.net/11573/1666122Test

المؤلفون: Ferretti J., Zeppilli M., Cristiani L., Majone M., Villano M.

المساهمون: Ferretti, J., Zeppilli, M., Cristiani, L., Majone, M., Villano, M.

مصطلحات موضوعية: acetate production, CO2 fixation, acetogenic inoculum

الوصف: In recent years there has been a growing interest in the potential use of autotrophic acetogenic bacteria to produce compounds of interest through CO2 fixation, representing an alternative solution to currently used CO2 storage technologies. This group of microorganisms are ubiquitous in nature and they are characterised by a Wood-Ljungdahl pathway that combines CO2 fixation with adenosine triphosphate (ATP) synthesis by using H2 as electron donor. In this work the autotrophic production of acetate by a pure colture of Acetobacterium woodii has been tested under hydrogenophilic or bioelectrochemical conditions. More in details, the hydrogenophilic tests were conducted at two different pH values (5.5 and 7.5) with an H2 partial pressure of 0.52 atm, while bioelectrochemical tests were performed at an applied cathodic potential of -0.90 V vs. SHE (Standard Hydrogen Electrode). The bioelectrochemical tests were set up in H-type reactors (250 mL), in which graphite rods were used as electrodic material and an anion exchange membrane served to separate the anodic and cathodic chambers while allowing anions migration for electroneutrality maintenance. The hydrogenophilic tests resulted in different kinetics depending on the applied pH value. The bioelectrochemical tests, performed at a pH value of 7.5, reached an acetate production rate 2 times higher than in the hydrogenophilic experiments at pH 7.5, as well as an increase in the efficiency of using the reducing power, suggesting an improvement in hydrogen uptake. At pH 5.5, on the other hand, production is improved by increasing the partial pressure of H2

العلاقة: volume:93; firstpage:223; lastpage:228; numberofpages:6; journal:CHEMICAL ENGINEERING TRANSACTIONS; https://hdl.handle.net/11573/1666121Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85134365907

الإتاحة: https://doi.org/10.3303/CET2293038Test
https://hdl.handle.net/11573/1666121Test

المؤلفون: Lai A., Astolfi M. L., Bertelli V., Agostinelli V. G., Zeppilli M., Majone M.

المساهمون: Lai, A., Astolfi, M. L., Bertelli, V., Agostinelli, V. G., Zeppilli, M., Majone, M.

مصطلحات موضوعية: Bioelectrochemical system, bioremediation, chlorinated solvent, Cr(VI) reduction

الوصف: A continuous-flow bioelectrochemical reactor was developed in a previous study to address the bioremediation of groundwater contaminated by trichloroethene (TCE). The present report investigated the applicability of the same system in the presence of Cr(VI) and its possible inhibitory effect on dehalorespiring bacterial populations. Preliminary batch tests were performed at the optimal cathodic reducing potential for the reductive dechlorination (RD) of TCE (-0.65 V vs. the standard hydrogen electrode) with two different dechlorinating microorganism consortia. The results demonstrated that Cr(VI) removal efficacy was increased by microorganisms that had been previously acclimatised to Cr(VI). Specifically, Cr(VI) was completely reduced only in the presence of acclimated microorganisms. The presence of chromate negatively affected RD performance, by either (i) limiting the TCE transformation to cis-dichloroethene at lower concentrations, or (ii) completely inhibiting RD at higher concentrations. In contrast, after the acclimation period, RD was extended down to vinyl chloride, which is the main TCE daughter product. Finally, the continuous flow reactor was fed by synthetic groundwater contaminated with TCE (50 μM) and Cr(VI) (45 μM), and the experimental results showed that Cr(VI) was completely reduced under RD conditions. Moreover, TCE removal was complete, with vinyl chloride and ethene as the main intermediates, thus indicating that chromate inhibition was decreased by Cr(VI) removal.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/32683048; info:eu-repo/semantics/altIdentifier/wos/WOS:000591724200006; volume:60; firstpage:27; lastpage:35; numberofpages:9; journal:NEW BIOTECHNOLOGY; http://hdl.handle.net/11573/1438381Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85090349696

الإتاحة: https://doi.org/10.1016/j.nbt.2020.06.006Test
http://hdl.handle.net/11573/1438381Test

المؤلفون: Zeppilli M., Dell'Armi E., Palumbo M. T., Petrangeli papini M

المساهمون: Zeppilli, M., Dell'Armi, E., Palumbo, M. T., Petrangeli papini, M

مصطلحات موضوعية: Chlorinated aliphatic hydrocarbons, reductive dechlorination, oxidative dechlorination

الوصف: Chlorinated Aliphatic Hydrocarbons (CAHs) as Perchloroethylene (PCE) and Trichloroethylene (TCE) are worldwide contaminants due to their uncorrected disposal and storage in the past years. An effective remediation strategy for CAHs contaminated groundwaters is the stimulation of dechlorinating microorganisms which can carry out reductive and oxidative reactions that allowed for the complete mineralization of CAHs. More in detail, dehalorespiring microorganisms can reduce PCE and TCE throughout reductive dechlorination reaction (RD) a step happening reaction that remove a chlorine atom from the carbon skeleton of the molecule and replaces it with a hydrogen ion. Hence, aerobic dechlorinating microorganisms oxidize low chlorinated compounds such as cis-dichloroethylene (cDCE) and vinyl chloride (VC) into CO2 using enzymes, such as monooxygenases, to produce instable molecules with oxygen atom like epoxides. The combination of reductive and oxidative dechlorination could maximize the microbial activities allowing to work on the preferred substrates and can be easily tuned by the adoption of bioelectrochemical systems. In these electrochemical devices, an electrodic material interact with so-called electroactive microorganisms, acting like electron acceptor or donor of the microbial metabolism. In this study, a sequential reductive/oxidative bioelectrochemical process developed by the combination in series of two membrane-less microbial electrolysis cells (MECs) has been applied for the treatment of a CAHs contaminated groundwater coming from a polluted site in northern Italy. More in detail, the study presents the development and the validation of the sequential bioelectrochemical process under laboratory conditions and the and subsequent scale-up of the process for a field. The investigation of the laboratory scale performance was conducted by synthetic and real contaminated groundwater while the design and the characterization of the scaled-up process have been obtained with real contaminated in a field test. The ...

العلاقة: volume:99; firstpage:703; lastpage:708; numberofpages:6; journal:CHEMICAL ENGINEERING TRANSACTIONS; https://hdl.handle.net/11573/1685963Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85163481252

الإتاحة: https://doi.org/10.3303/CET2399118Test
https://hdl.handle.net/11573/1685963Test

المؤلفون: Zeppilli M., Dell'Armi E., Cristiani L., Papini, Majone M.

المساهمون: Zeppilli, M., Dell'Armi, E., Cristiani, L., PETRANGELI PAPINI, Marco, Majone, M.

مصطلحات موضوعية: bioelectrochemical system, bioremediation, oxidative dechlorination, reductive dechlorination

الوصف: An innovative bioelectrochemical reductive/oxidative sequential process was developed and tested on a laboratory scale to obtain the complete mineralization of perchloroethylene (PCE) in a synthetic medium. The sequential bioelectrochemical process consisted of two separate tubular bioelectrochemical reactors that adopted a novel reactor configuration, avoiding the use of an ion exchange membrane to separate the anodic and cathodic chamber and reducing the cost of the reactor. In the reductive reactor, a dechlorinating mixed inoculum received reducing power to perform the reductive dechlorination of perchloroethylene (PCE) through a cathode chamber, while the less chlorinated daughter products were removed in the oxidative reactor, which supported an aerobic dechlorinating culture through in situ electrochemical oxygen evolution. Preliminary fluid dynamics and electrochemical tests were performed to characterize both the reductive and oxidative reactors, which were electrically independent of each other, with each having its own counterelectrode. The first continuous-flow potentiostatic run with the reductive reactor (polarized at -450 mV vs SHE) resulted in obtaining 100% ± 1% removal efficiency of the influent PCE, while the oxidative reactor (polarized at +1.4 V vs SHE) oxidized the vinyl chloride and ethylene from the reductive reactor, with removal efficiencies of 100% ± 2% and 92% ± 1%, respectively.

العلاقة: info:eu-repo/semantics/altIdentifier/wos/WOS:000507378600152; volume:11; issue:12; numberofpages:16; journal:WATER; info:eu-repo/grantAgreement/EC/H2020/826244; http://hdl.handle.net/11573/1348028Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85076717983

الإتاحة: https://doi.org/10.3390/w11122579Test
http://hdl.handle.net/11573/1348028Test

المؤلفون: Zeppilli, M., Cristiani, L., Ferretti, J., Majone, M., Villano, M.

مصطلحات موضوعية: CO2 fixation, acetate production, acetogenic inoculum

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=od______3686::756398334c6d0cb25198ef9dfaf5e657Test
https://hdl.handle.net/11573/1666124Test

المؤلفون: Ferretti, J., Zeppilli, M., Cristiani, L., Majone, M., Villano, M.

مصطلحات موضوعية: CO2 fixation, acetate production, acetogenic inoculum

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=od______3686::912f0f18c63b8b5b1356c85b685e621fTest
https://hdl.handle.net/11573/1666121Test

المؤلفون: Dell'Armi, E., Rossi, M. M., Zeppilli, M., Majone, M., Petrangeli Papini, M.

مصطلحات موضوعية: reductive dechlorination, oxidative dechlorination, bioelectroremediation, chlorinated ethylenes

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=od______3686::8b7915f13dd1fa14869a5a9fcef56dd5Test
https://hdl.handle.net/11573/1666122Test