المؤلفون: Zepelin, Margarete Borg-von, Kunz, Luisa, Rüchel, Reinhard, Reichard, Utz, Weig, Michael, Groß, Uwe

المصدر: Journal of Antimicrobial Chemotherapy ; volume 60, issue 2, page 424-428 ; ISSN 1460-2091 0305-7453

مصطلحات موضوعية: Infectious Diseases, Pharmacology (medical), Pharmacology, Microbiology (medical)

الإتاحة: https://doi.org/10.1093/jac/dkm145Test
http://academic.oup.com/jac/article-pdf/60/2/424/2096976/dkm145.pdfTest

المؤلفون: Schaller, Martin, Thoma-Greber, Eva, Korting, Hans C., Hube, Bernhard, Ollert, Markus W., Schäfer, Wilhelm, Zepelin, Margarete Borg-von

المصدر: Journal of Investigative Dermatology ; volume 112, issue 3, page 383-386 ; ISSN 0022-202X

مصطلحات موضوعية: Cell Biology, Dermatology, Molecular Biology, Biochemistry

الإتاحة: https://doi.org/10.1046/j.1523-1747.1999.00525.xTest
https://api.elsevier.com/content/article/PII:S0022202X15404300?httpAccept=text/plainTest
https://api.elsevier.com/content/article/PII:S0022202X15404300?httpAccept=text/xmlTest

المؤلفون: Gruber, Andreas, Lukasser-Vogl, Elisabeth, Zepelin, Margarete Borg-von, Dierich, Manfred P., Würzner, Reinhard

مصطلحات موضوعية: Major Articles

الوصف: Previously, it has been shown that human immunodeficiency virus (HIV)-1 envelope proteins gp160 and gp41 bind to Candida albicans . Whether this interaction affects candidal virulence in vitro was investigated. HIV-1 gp160 or gp120 treatment of C. albicans significantly altered neither growth nor phospholipase activity of the fungus. However, treatment of C. albicans with gp160, but not with gp120, led to an elevation of free and cell-bound aspartate proteinase. In addition, culture supernatants obtained from C. albicans treated with gp160 or gp41, but not with gp120, showed a strong increase in proteinase activity. Finally, C. albicans viable yeast cells treated with gp160 or gp41 and serum were phagocytosed by polymorphonuclear leukocytes to a lesser extent than was C. albicans treated with gp120 and serum or serum alone. These findings suggest that the interaction between HIV-1 gp160 and C. albicans may promote the virulence of C. albicans in HIV-1-positive patients.

وصف الملف: text/html

العلاقة: http://jid.oxfordjournals.org/cgi/content/short/177/4/1057Test; http://dx.doi.org/10.1086/515231Test

الإتاحة: https://doi.org/10.1086/515231Test
http://jid.oxfordjournals.org/cgi/content/short/177/4/1057Test

المؤلفون: Zepelin, Margarete Borg von, Kahl, D., Hoech, R., Ruechel, Reinhard, Reichard, Utz, Weig, Michael S., Gross, U.

العلاقة: https://resolver.sub.uni-goettingen.de/purl?gro-2/55534Test; 000258408900069

الإتاحة: https://resolver.sub.uni-goettingen.de/purl?gro-2/55534Test

المؤلفون: Perl, Thorsten, Juenger, Melanie, Vautz, Wolfgang, Nolte, Juergen, Kuhns, Martin, Zepelin, Margarete Borg-von, Quintel, Michael

الوصف: Volatile metabolites of Aspergillus fumigatus and Candida species can be detected by gas chromatography/mass spectrometry (GC/MS). A multi-capillary column - ion mobility spectrometer (MCC-IMS) was used in this study to assess volatile organic compounds (VOCs) in the headspace above A. fumigatus and the four Candida species Candida albicans, Candida parapsilosis, Candida glabrata and Candida tropicalis in an innovative approach, validated for A. fumigatus and C. albicans by GC/MS analyses. For the detection of VOCs, a special stainless steel measurement chamber for the microbial cultures was used. The gas outlet was either attached to MCC-IMS or to adsorption tubes (Tenax GR) for GC/MS measurements. Isoamyl alcohol, cyclohexanone, 3-octanone and phenethylalcohol can be described as discriminating substances by means of GC/MS. With MCC-IMS, the results for 3-octanone and phenethylalcohol are concordant and additionally to GC/MS, ethanol and two further compounds (p_0642_1/p_683_1 and p_705_3) can be described. Isoamyl alcohol and cyclohexanone were not properly detectable with MCC-IMS. The major advantage of the MCC-IMS system is the feasibility of rapid analysis of complex gas mixtures without pre-concentration or preparation of samples and regardless of water vapour content in an online setup. Discrimination of fungi on genus level of the investigated germs by volatile metabolic profile and therefore detection of VOC is feasible. However, a further discrimination on species level for Candida species was not possible.

العلاقة: https://resolver.sub.uni-goettingen.de/purl?gro-2/21692Test; 000296243700024

الإتاحة: https://doi.org/10.1111/j.1439-0507.2011.02037.xTest
https://resolver.sub.uni-goettingen.de/purl?gro-2/21692Test

المؤلفون: Sennhenn-Kirchner, Sabine, Weustermann, Sascha, Mergeryan, Hamparsum, Jacobs, Hans Georg, Zepelin, Margarete Borg-von, Kirchner, Bernhard

الوصف: The aim of the in vitro study was to evaluate the decontamination potential of common antiseptic solutions for heat-sensitive implantological drill guide templates. One hundred implantologists were evaluated on the basis of a questionnaire for their measures of disinfection. On the basis of these results, 80% alcohol, Octenidine 0.1%, and Chlorhexidine 0.12% were tested in an in vitro model for their decontamination efficacy for heat-sensitive plastic material infected with Pseudomonas aeruginosa, Acinetobacter baumannii, Enterococcus faecalis, Enterococcus faecium, Staphylococcus aureus, Enterobacter cloacae, Escherichia coli, and Candida albicans. The microorganisms were selected on the basis of results of environmental testing of dental laboratories. The results of the questionnaire revealed that Chlorhexidine was used by 30%, 80% alcohol by 23%, and Octenidine by 7% of the dentists. Using the in vitro model, with the exception of S. aureus, Chlorhexidine was not able to completely eliminate the microorganisms after 15 min of application. In contrast, the treatment with Octenidine revealed no further growth of the tested microorganisms after that time. The 80% alcohol was more efficient. No growth of microorganisms could be detected in any of the tests after 5 min of incubation. On the basis of our results and due to the fact that suitable installations for sterilization were hardly used by the dental practitioners, the disinfection of templates should be preferentially performed with 80% alcohol or Octenidine using an incubation time of 15 min with ultrasonication.

العلاقة: https://resolver.sub.uni-goettingen.de/purl?gro-2/54097Test; 000255535900012

الإتاحة: https://doi.org/10.1007/s00784-007-0153-9Test
https://resolver.sub.uni-goettingen.de/purl?gro-2/54097Test

المؤلفون: Zepelin, Margarete Borg‐von

المصدر: Mycoses ; volume 51, issue s3, page 17-19 ; ISSN 0933-7407 1439-0507

الإتاحة: https://doi.org/10.1111/j.1439-0507.2008.01580.xTest

المؤلفون: Michel Monod, Zepelin Margarete Borg-von

المصدر: Biological chemistry. 383(7-8)

مصطلحات موضوعية: chemistry.chemical_classification, Virulence Factors, Phagocytosis, Clinical Biochemistry, Candidiasis, Virulence, Biology, Biochemistry, Corpus albicans, Oropharyngeal Candidiasis, Microbiology, Substrate Specificity, Pathogenesis, Enzyme, Immune system, chemistry, Immunology, HIV Protease Inhibitor, Animals, Aspartic Acid Endopeptidases, Humans, Molecular Biology, Candida

الوصف: Candida infections have emerged as a significant medical problem during the last few decades. Among the different virulence traits of C. albicans, secreted proteolytic activity has been intensively investigated. Pathogenesis of the various forms of candidiasis was shown to be associated with the differential and temporal regulation of the expression of genes coding for secreted aspartic proteases (Sap). These enzymes act as cytolysins in macrophages after phagocytosis of Candida, are present in tissue penetration and are also involved in adherence to epithelial cells. Since the introduction of new antiretroviral therapeutics such as HIV protease inhibitors, oropharyngeal candidiasis is less often observed in AIDS patients. Different HIV aspartic protease inhibitors were able to inhibit the C. albicans Saps involved in adherence. The lower rates of oropharyngeal candidiasis observed in individuals receiving antiretroviral combination therapy could reflect not only an improvement in the immune system but also direct inhibition of Candida Saps by HIV protease inhibitors. Therefore, the development of specific aspartic protease inhibitors might be of interest for the inhibition of candidiasis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21e377335250bb6f1872ae6b46edc498Test
https://pubmed.ncbi.nlm.nih.gov/12437091Test

المؤلفون: Zepelin, Margarete Borg‐von, Eucker, Jan, Rüchel, R.

المصدر: Mycoses ; volume 40, issue s1, page 64-72 ; ISSN 0933-7407 1439-0507

الوصف: Zusammenfassung. Die Beteiligung heterologer saurer sekretorischer Candida ‐Proteinasen bei der Adhärenz des nicht‐proteinase‐sezernierenden Stammes C. tropicalis DSM 4959 an epitheloiden Zellen (Vero‐Li‐nie) wurde untersucht. Dazu wurden die Proteinasen folgender Candida ‐Stämme verwendet: C. albicans ATCC 10261 (Serotyp A), C. albicans ATCC 48867 (Serotyp B), C. tropicalis DSM 4238. Die Untersuchung wurde mit dem bereits beschriebenen Adhärenztest in‐vitro durchgeführt [1], der aus folgenden prinzipiellen Schritten aufgebaut ist: Candida ‐Proteinasen und C. tropicalis ‐Blastoconidien wurden gemeinsam mit Verozellen in Mikrotestplatten in Phosphatpuffern im Bereich von pH 4,0 bis pH 7,0 inkubiert. Der Nachweis adhärenter Candida‐Zellen wurde in Anlehnung an Filler und Mitarbeiter [2] mit Hilfe von anti‐Candida‐Man‐noprotein‐Antikörpern sowie eines sekundären anti‐Kaninchen‐Peroxidase‐Konjugats durchgeführt. Im Vergleich zu Kontrollansätzen mit denaturierten Proteinasen zeigten die photometrischen Auswertungen adhärenter C.‐tropicalis ‐Zellen, daß die eingesetzten heterologen Candida ‐Proteinasen die Adhärenz unter optimalen Bedingungen um etwa 50% verstärkten. Das Optimum dieser Adhärenzverstärkung lag mit pH 5,5 außerhalb des allgemeinen Aktivitätsoptimums von Candida ‐Proteinasen (pH 3). Der Aufreinigungsgrad der Proteinasen hatte keinen deutlichen Einfluß auf die Adhärenz. Die Spezifität der proteinase‐abhängigen Adhärenz‐Verstärkung konnte mit Hilfe des Inhibitors Pepstatin A gezeigt werden. Die Adhärenz in Pepstatin‐A‐Proteinase‐Ansätzen lag im Bereich der Kontrollen mit denaturierter Proteinase. Unsere Ergebnisse lassen eine Funktion von sekretorischen Candida ‐Proteinasen bei der Adhärenz von Candida ‐Blasto‐conidien an Epithelien vermuten. Summary. The influence of the heterologous acid secretory Candida proteinases on the adherence of the non‐proteinase secreting strain of C. tropicalis DSM 4959 to epitheloid cells (vero line) was examined. The proteinases of the following Candida strains were used: C. ...

الإتاحة: https://doi.org/10.1111/j.1439-0507.1997.tb00544.xTest

المؤلفون: Zepelin, Margarete Borg‐von

المصدر: Mycoses ; volume 38, issue 9-10, page 339-347 ; ISSN 0933-7407 1439-0507

الوصف: Summary. We describe an assay based on photometric analysis for the measurement of adherence of Candida species to epithelial target cells (Vero cell line). Adherent Candida cells were detected by staining the cells with the fluorescent dye Calcofluor white (CFW), which binds to chitin and glucan in the yeasts. The tests were performed on microtest plates, which were analysed automatically by fluorescence plate readers. The assay is based on the following steps: (i) coating of the microtest plates with target cells (e.g. Vero cells); (ii) infection with Candida : (iii) staining of Candida with CFW; (iv) rinsing to remove non‐adherent Candida cells and unbound dye; (v) detection of adherent fluorescent Candida cells. The test was able to detect 4times10 4 cells ml ‐1 . The standard deviation was ±8%. Day‐to‐day variation was ± 10% at most. The adherence of strains of different Candida species was assayed by a standard procedure. The results confirmed the order of adherence, with C. albicans ranking first, followed by C. tropicalis, C. parapsilosis and C. glabrata . Zusammenfassung. Ein photometrischer Test zur Messung der Adhärenz von Candida‐Species an epithelialen Zielzellen (Vero‐Zellen) wird beschrieben. Adhärente Candida Zellen werden mit Hilfe des Fluoreszenz‐Farbstoffes Calcofluor Weiβ (CFW), der an Chitin und Glucan bindet, nach‐gewiesen. Der Test Kann in Mikrotestplatten durchgeührt werden und mittels automati‐scher Fluoreszenz‐Plattenphotometer ausgewertet werden. Er umfaßt die folgenden Schritte: (i) Beschichtung der Mikrotestplatte mit Zielzellen (Vero‐Zellen); (ii) Infektion mit Candida; (iii) Färbung der Candida‐Zellen mit CFW; (iv) Ent‐fernung nicht‐adhärenter Candida‐Zellen und ungebundener Farbe; (v) Nachweis adhärenter fluoreszierender Candida‐Zellen. Mit dem Test ist es möglich, bis zu 4times 10 4 eingesetzter Hefen pro Milliliter nachzuweisen. Die üiblichen Testpara‐meter wurden bestimmt. Die Standardabweichung lag bei ±8%. Die Schwankungen von Tag zu Tag lagen bei maximal ±10%. Der Test wurde ...

الإتاحة: https://doi.org/10.1111/j.1439-0507.1995.tb00062.xTest