المؤلفون: Seyed Askar Roghani, Ramin Lotfi, Masood Ghasemzade Soroush, Ali Khorasanizadeh, Parisa Feizollahi, Shirin Assar, Parviz Soufivand, Mehran Pournazari, Zahra Mohammadi Kish, Mahdi Taghadosi

المصدر: Immunity, Inflammation and Disease, Vol 11, Iss 12, Pp n/a-n/a (2023)

مصطلحات موضوعية: anti‐CCP, CCR6, DAS‐28, rheumatoid arthritis, RORγt, Immunologic diseases. Allergy, RC581-607

الوصف: Abstract Objectives The significance of T helper 17 (Th17) cells in the pathogenesis of rheumatoid arthritis (RA) has recently been demonstrated in many studies. Retinoic acid receptor‐related orphan receptor γt (RORγt) is a transcription factor that is specifically involved in the generation of Th17 cells. Besides, the chemokine receptor CCR6, the receptor for CCL20, is characteristically expressed by these cells. Considering the pivotal roles of Th17 cells in RA pathogenesis, in this study, we assessed the gene expression of CCR6 and RORγt in the peripheral blood leukocytes of new case RA patients. Also, we evaluated their association with anticyclic citrullinated peptide (anti‐CCP) antibodies and disease activity. Methods Forty‐five new case RA patients and 45 healthy persons have been recruited in this investigation. The gene expression of CCR6 and RORγt was evaluated by quantitative real‐time PCR (qRT‐PCR), and anti‐CCP antibodies plasma levels were measured using the enzyme‐linked immunosorbent assay (ELISA) technique. Disease activity was measured according to the disease activity score‐28 (DAS‐28) formula. Results The gene expression of CCR6 and RORγt increased remarkably in new case RA patients compared to healthy controls (p

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2050-4527Test

الوصول الحر: https://doaj.org/article/055f8648914f48298bdbddc09e635e73Test

المؤلفون: Parisa Feizollahi, Parviz Soufivand, Seyed Askar Roghani, Fariborz Bahrehmand, Bijan Soleymani, Mehran Pournazari, Zahra Mohammadi Kish, Mahdi Taghadosi, Shirin Assar

المصدر: Clinical rheumatology. 41(1)

مصطلحات موضوعية: medicine.medical_specialty, medicine.medical_treatment, Enzyme-Linked Immunosorbent Assay, Gastroenterology, Peptides, Cyclic, Pathogenesis, Arthritis, Rheumatoid, Rheumatology, Rheumatoid Factor, Internal medicine, medicine, Rheumatoid factor, Humans, Pathological, Autoantibodies, Chemokine CCL20, biology, business.industry, General Medicine, medicine.disease, CCL20, Cytokine, Rheumatoid arthritis, biology.protein, Antibody, business, Laboratories, Biomarkers

الوصف: Rheumatoid arthritis (RA) is a systemic autoimmune disease that primarily affects small joints. The impaired chemokine and cytokine responses are essential pathological mechanisms for the RA clinical presentation. Given the role of chemokines and inflammatory reactions in RA pathogenesis, we evaluate the association between the plasma concentration of CCL20 with the clinical and laboratory parameters in newly diagnosed RA patients.Forty-five newly diagnosed RA patients and forty-five healthy subjects were enrolled in this study. The plasma levels of CCL20, rheumatoid factor, and anti-citrullinated peptide antibodies were measured using the enzyme-linked immunosorbent assay (ELISA) technique.The plasma levels of CCL20 were increased significantly in RA patients compared to the healthy controls (p 0.0001). There was a positive correlation between CCL20 and RF, anti-CCP, ESR, and DAS-28 (p 0.0001, r = 0.669; p 0.015, r = 0.358; p 0.0001, r = 0.586; p 0.0001, r = 0.769).The increased plasma levels of CCL20 in newly diagnosed RA patients may contribute to RA pathogenesis, and it is in association with clinical and laboratory parameters. Key Points • CCL20 has a contribution to the early phase of RA.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d33c2e8d4d77c72ece85cb4ed1bd62b8Test
https://pubmed.ncbi.nlm.nih.gov/34477989Test