المؤلفون: Tianwu Chen, Yu Dong, Yunxia Li, Shiyi Chen

المصدر: Journal of Orthopaedic Translation, Vol 47, Iss , Pp 29-38 (2024)

مصطلحات موضوعية: Allograft, Anterior cruciate ligament, Artificial ligament, Psychological recovery, Return to sport, Revision, Diseases of the musculoskeletal system, RC925-935

الوصف: Background: Research on return to sport and psychological recovery in anterior cruciate ligament (ACL) revision remains scarce. The clinical efficacy of artificial ligament in ACL revision requires further exploration. Our objectives were (1) to compare the midterm clinical outcomes of artificial ligament versus allogenic tendon graft in ACL revision and (2) to analyze the effects of employing artificial ligament on return to sport and psychological recovery in ACL revision. Methods: This cohort study included the cases receiving ACL revision from 2014 to 2021 in Sports Medicine Department of Huashan Hospital. The grafts used were Ligament Advanced Reinforcement System (LARS) and ATT allograft. We recorded patients' baseline data. The final follow-up assessment included subjective scales, physical examination, and return to sport status. We recorded the rates and timings of return to sport. Subjective scales included the 2000 International Knee Documentation Committee (IKDC) subjective score, Lysholm Knee Scaling Score (LKSS), Knee injury and Osteoarthritis Outcome Score (KOOS), Tegner activity score, Marx activity rating score, and Anterior Cruciate Ligament-Return to Sport after Injury (ACL-RSI). Anterior knee stability was assessed using the KT-1000 arthrometer. Results: Fifty cases (LARS group: 27; ATT group: 23) enrolled and 45 (LARS group: 23; ATT group: 22) completed evaluations with a median follow-up period of 49 months. At recent follow-up, LARS group outperformed in knee stability (1.0 ± 1.9 mm vs. 2.6 ± 3.0 mm, P = 0.039), confidence (86.7 ± 12.4 vs. 69.4 ± 18.6, P

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2214031X24000457Test; https://doaj.org/toc/2214-031XTest

الوصول الحر: https://doaj.org/article/9d66a7c0741d48718a44e76f521ca758Test

المؤلفون: Jing Wang, Xinyan Xue, Xingtao Zhao, Lin Luo, Juan Liu, Shu Dai, Fang Zhang, Rui Wu, Yanfang Liu, Cheng Peng, Yunxia Li

المصدر: Journal of Advanced Research, Vol 60, Iss , Pp 183-200 (2024)

مصطلحات موضوعية: Forsythiaside A, Acute lung injury, Gut-lung axis, LPS and TNF-α, TLR4/MAPK/NF-κB and NF-κB/MLCK/MLC2, PPAR-γ/RXR-α, Medicine (General), R5-920, Science (General), Q1-390

الوصف: Introduction: Acute lung injury (ALI) is a lung disease characterized by inflammation and still requires further drug development. Forsythiaside A as the active compound of Forsythiae Fructus has the therapeutic potential for ALI. Objective: To investigate the mechanism of forsythiaside A in treating ALI through PPAR-γ and its conjugate RXR-α based on gut-lung axis. Methods: This study constructed in vitro and in vivo injury models using LPS and TNF-α. Forsythiaside A was used for the drug treatment, and RXR-α inhibitor UVI3003 was used to interfere with PPAR-γ/RXR-α complexes in the cells. HE staining was used for histopathological examination. Serum endotoxin contents were determined using limulus lysate kit. IHC staining and Western blot were conducted to assess the protein expressions. ELISA was applied to examine the content of pro-inflammatory cytokines in the cell supernatants. The protein interactions were analyzed via CO-IP. Results: In vivo results showed that forsythiaside A regulated PPAR-γ/RXR-α and inhibited TLR4/MAPK/NF-κB and MLCK/MLC2 signal pathways, thus inhibiting inflammation and epithelial barrier damages of lung and colon in ALI mice induced by intratracheal LPS. PPAR-γ/RXR-α were promoted by forsythiaside A in lungs, whereas inhibited by forsythiaside A in colons. Additionally, in vitro results showed that forsythiaside A suppressed inflammation and epithelial barrier damages in macrophages and lung/colon epithelial cells, by manipulating PPAR-γ/RXR-α to suppress the LPS- and TNF-α-induced activation of TLR4/MAPK/NF-κB and NF-κB/MLCK/MLC2 signal pathways. Moreover, further mechanism study indicated that forsythiaside A showed a cell-specific regulatory effect on PPAR-γ/RXR-α complex. Specifically, the PPAR-γ/RXR-α protein interactions were promoted by forsythiaside A in LPS-induced macrophages RAW264.7 and TNF-α-induced lung epithelial cells A549, but inhibited by forsythiaside A in TNF-α-induced colon epithelial cells SW620. Conclusion: In the treatment of ALI, Forsythiaside A inhibited inflammation and epithelial barrier damages of lung and colon through its regulation on PPAR-γ/RXR-α complex.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2090123223002229Test; https://doaj.org/toc/2090-1232Test

الوصول الحر: https://doaj.org/article/9377d5549bd34fd090b25d337a7363c7Test

المؤلفون: Lijuan Sha, Lijun Tian, Lifen Sha, Yunxia Li, Xuejie Wang, Peng Hu, Qisheng Liang, Baoyun Zong, Pengzhen Duan, Hai Cheng

المصدر: Communications Earth & Environment, Vol 5, Iss 1, Pp 1-11 (2024)

مصطلحات موضوعية: Geology, QE1-996.5, Environmental sciences, GE1-350

الوصف: Abstract The isotopic composition has long been used to investigate the factors influencing precipitation, whereas the variations of event-based precipitation isotopes caused by moisture transition and synoptic meteorological conditions remain limited. Here we present triple oxygen and hydrogen isotopes in event-based precipitation during West China Autumn Precipitation to evaluate the influence of various moisture sources in the hydrological process. Isotopes δ18O, δ17O, and δD peak with convective precipitation at the onset stage, then drop to their lowest amid stratiform precipitation during the middle stage, and rise again towards the end. In contrast, Δ′17O levels remain elevated throughout the mid-stage of West China Autumn Precipitation compared to the onset and end stages. These isotopic variations, coupled with moisture analysis, reveal a distinct moisture source transition from the West Pacific Ocean to the westerly domain during West China Autumn Precipitation accompanied by the retreat of the Asian summer monsoon from Northwest China.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2662-4435Test

الوصول الحر: https://doaj.org/article/0e2b25ad6f5e4413916c56a5d67f1116Test

المؤلفون: Ying Zhang, Le Xue, Shuoyan Zhang, Jiacheng Yang, Qi Zhang, Min Wang, Luyao Wang, Mingkai Zhang, Jiehui Jiang, Yunxia Li, for the Alzheimer’s Disease Neuroimaging Initiative

المصدر: Alzheimer’s Research & Therapy, Vol 16, Iss 1, Pp 1-16 (2024)

مصطلحات موضوعية: Alzheimer’s disease, Imaging biomarkers, Functional connectivity, Graph neural network, Multi-site, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571, Neurology. Diseases of the nervous system, RC346-429

الوصف: Abstract Background Functional connectivity (FC) biomarkers play a crucial role in the early diagnosis and mechanistic study of Alzheimer’s disease (AD). However, the identification of effective FC biomarkers remains challenging. In this study, we introduce a novel approach, the spatiotemporal graph convolutional network (ST-GCN) combined with the gradient-based class activation mapping (Grad-CAM) model (STGC-GCAM), to effectively identify FC biomarkers for AD. Methods This multi-center cross-racial retrospective study involved 2,272 participants, including 1,105 cognitively normal (CN) subjects, 790 mild cognitive impairment (MCI) individuals, and 377 AD patients. All participants underwent functional magnetic resonance imaging (fMRI) and T1-weighted MRI scans. In this study, firstly, we optimized the STGC-GCAM model to enhance classification accuracy. Secondly, we identified novel AD-associated biomarkers using the optimized model. Thirdly, we validated the imaging biomarkers using Kaplan–Meier analysis. Lastly, we performed correlation analysis and causal mediation analysis to confirm the physiological significance of the identified biomarkers. Results The STGC-GCAM model demonstrated great classification performance (The average area under the curve (AUC) values for different categories were: CN vs MCI = 0.98, CN vs AD = 0.95, MCI vs AD = 0.96, stable MCI vs progressive MCI = 0.79). Notably, the model identified specific brain regions, including the sensorimotor network (SMN), visual network (VN), and default mode network (DMN), as key differentiators between patients and CN individuals. These brain regions exhibited significant associations with the severity of cognitive impairment (p

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1758-9193Test

الوصول الحر: https://doaj.org/article/50614f0149e34e64ad5d8e1b00cba4a7Test

المؤلفون: Mo Chen, Yuzhou Chen, Sijia Feng, Shixian Dong, Luyi Sun, Huizhu Li, Fuchun Chen, Nguyen Thi Kim Thanh, Yunxia Li, Shiyi Chen, You Wang, Jun Chen

المصدر: Engineering, Vol 33, Iss , Pp 283-294 (2024)

مصطلحات موضوعية: In vivo, Short-wave infrared, Skeletal muscle, Macrophage, Regeneration, Engineering (General). Civil engineering (General), TA1-2040

الوصف: Skeletal muscle has a robust regeneration ability that is impaired by severe injury, disease, and aging, resulting in a decline in skeletal muscle function. Therefore, improving skeletal muscle regeneration is a key challenge in treating skeletal muscle-related disorders. Owing to their significant role in tissue regeneration, implantation of M2 macrophages (M2Mø) has great potential for improving skeletal muscle regeneration. Here, we present a short-wave infrared (SWIR) fluorescence imaging technique to obtain more in vivo information for an in-depth evaluation of the skeletal muscle regeneration effect after M2Mø transplantation. SWIR fluorescence imaging was employed to track implanted M2Mø in the injured skeletal muscle of mouse models. It is found that the implanted M2Mø accumulated at the injury site for two weeks. Then, SWIR fluorescence imaging of blood vessels showed that M2Mø implantation could improve the relative perfusion ratio on day 5 (1.09 ± 0.09 vs 0.85 ± 0.05; p = 0.01) and day 9 (1.38 ± 0.16 vs 0.95 ± 0.03; p = 0.01) post-injury, as well as augment the degree of skeletal muscle regeneration on day 13 post-injury. Finally, multiple linear regression analyses determined that post-injury time and relative perfusion ratio could be used as predictive indicators to evaluate skeletal muscle regeneration. These results provide more in vivo details about M2Mø in skeletal muscle regeneration and confirm that M2Mø could promote angiogenesis and improve the degree of skeletal muscle repair, which will guide the research and development of M2Mø implantation to improve skeletal muscle regeneration.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2095809923002369Test; https://doaj.org/toc/2095-8099Test

الوصول الحر: https://doaj.org/article/13d1b6628ca64809868cad1646463905Test

المؤلفون: Kaijun Wang, Jie Ma, Yunxia Li, Qi Han, Zhangzheng Yin, Miao Zhou, Minyi Luo, Jiayi Chen, Siting Xia

المصدر: Frontiers in Nutrition, Vol 11 (2024)

مصطلحات موضوعية: Artemisia argyi, high fat, lipid, gut, microbiota, Nutrition. Foods and food supply, TX341-641

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fnut.2024.1416210/fullTest; https://doaj.org/toc/2296-861XTest

الوصول الحر: https://doaj.org/article/ca3224b1dbd0473e8d90503bd02a9439Test

المؤلفون: Jingnan Sun, Zengmai Xie, Yike Sun, Anruo Shen, Renren Li, Xiao Yuan, Bai Lu, Yunxia Li

المصدر: MedComm, Vol 5, Iss 5, Pp n/a-n/a (2024)

مصطلحات موضوعية: amyloid beta protein, cerebrospinal fluid, EEG, MRI, Medicine

الوصف: Abstract Alzheimer's disease (AD) constitutes a neurodegenerative disorder marked by a progressive decline in cognitive function and memory capacity. The accurate diagnosis of this condition predominantly relies on cerebrospinal fluid (CSF) markers, notwithstanding the associated burdens of pain and substantial financial costs endured by patients. This study encompasses subjects exhibiting varying degrees of cognitive impairment, encompassing individuals with subjective cognitive decline, mild cognitive impairment, and dementia, constituting a total sample size of 82 participants. The primary objective of this investigation is to explore the relationships among brain atrophy measurements derived from magnetic resonance imaging, atypical electroencephalography (EEG) patterns, behavioral assessment scales, and amyloid β‐protein (Aβ) indicators. The findings of this research reveal that individuals displaying reduced Aβ1‐42/Aβ‐40 levels exhibit significant atrophy in the frontotemporal lobe, alongside irregularities in various parameters related to EEG frequency characteristics, signal complexity, inter‐regional information exchange, and microstates. The study additionally endeavors to estimate Aβ1‐42/Aβ‐40 content through the application of a random forest algorithm, amalgamating structural data, electrophysiological features, and clinical scales, achieving a remarkable predictive precision of 91.6%. In summary, this study proposes a cost‐effective methodology for acquiring CSF markers, thereby offering a valuable tool for the early detection of AD.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2688-2663Test

الوصول الحر: https://doaj.org/article/e43addc516134a91b8031bf904c26f90Test

المؤلفون: Miaomiao Teng, Yunxia Li, Xiaoli Zhao, Jason C. White, Lihui Zhao, Jiaqi Sun, Wentao Zhu, Fengchang Wu

المصدر: Microbiome, Vol 11, Iss 1, Pp 1-15 (2023)

مصطلحات موضوعية: Polystyrene nanoplastics, Vitamin D, Brain, Viruses, Zebrafish, Microbial ecology, QR100-130

الوصف: Abstract Background Many studies have investigated how nanoplastics (NPs) exposure mediates nerve and intestinal toxicity through a dysregulated brain-gut axis interaction, but there are few studies aimed at alleviating those effects. To determine whether and how vitamin D can impact that toxicity, fish were supplemented with a vitamin D-low diet and vitamin D-high diet. Results Transmission electron microscopy (TEM) showed that polystyrene nanoplastics (PS-NPs) accumulated in zebrafish brain and intestine, resulting in brain blood–brain barrier basement membrane damage and the vacuolization of intestinal goblet cells and mitochondria. A high concentration of vitamin D reduced the accumulation of PS-NPs in zebrafish brain tissues by 20% and intestinal tissues by 58.8% and 52.2%, respectively, and alleviated the pathological damage induced by PS-NPs. Adequate vitamin D significantly increased the content of serotonin (5-HT) and reduced the anxiety-like behavior of zebrafish caused by PS-NPs exposure. Virus metagenome showed that PS-NPs exposure affected the composition and abundance of zebrafish intestinal viruses. Differentially expressed viruses in the vitamin D-low and vitamin D-high group affected the secretion of brain neurotransmitters in zebrafish. Virus AF191073 was negatively correlated with neurotransmitter 5-HT, whereas KT319643 was positively correlated with malondialdehyde (MDA) content and the expression of cytochrome 1a1 (cyp1a1) and cytochrome 1b1 (cyp1b1) in the intestine. This suggests that AF191073 and KT319643 may be key viruses that mediate the vitamin D reduction in neurotoxicity and immunotoxicity induced by PS-NPs. Conclusion Vitamin D can alleviate neurotoxicity and immunotoxicity induced by PS-NPs exposure by directionally altering the gut virome. These findings highlight the potential of vitamin D to alleviate the brain-gut-virome disorder caused by PS-NPs exposure and suggest potential therapeutic strategies to reduce the risk of NPs toxicity in aquaculture, that is, adding adequate vitamin D to diet. Video Abstract

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2049-2618Test

الوصول الحر: https://doaj.org/article/1dcdaecb567842cb8a9f49edbbf8503bTest

المؤلفون: Jie Ma, Miao Zhou, Zehe Song, Yuankun Deng, Siting Xia, Yunxia Li, Xingguo Huang, Dingfu Xiao, Yulong Yin, Jie Yin

المصدر: Microbiome, Vol 11, Iss 1, Pp 1-13 (2023)

مصطلحات موضوعية: Gut fungi, Lipid metabolism, Clec7a, Microbial ecology, QR100-130

الوصف: Abstract Background Compared to that of bacteria, the role of gut fungi in obesity development remains unknown. Results Here, alterations in gut fungal biodiversity and composition were confirmed in obese pig models and high-fat diet (HFD)-fed mice. Antifungal drugs improved diet-induced obesity, while fungal reconstruction by cohousing or fecal microbiota transplantation maintained the obese phenotype in HFD-fed mice. Fungal profiling identified 5 fungal species associated with obesity. Specifically, Ascomycota_sp. and Microascaceae_sp. were reduced in obese mice and negatively correlated with fat content. Oral supplementation with fungi was sufficient to prevent and treat diet-induced obesity. Clec7a, which is involved in fungal recognition, was highly expressed in HFD-fed mice. The Clec7a agonist accelerated diet-induced obesity, while Clec7a deficieny in mice resulted in resistance to diet-induced obesity and blocked the anti-obese effect of antifungal drugs and fungi. Conclusions Taken together, these results indicate that gut fungi/Clec7a signaling is involved in diet-induced obesity and may have therapeutic implications as a biomarker for metabolic dysregulation in humans. Video Abstract

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2049-2618Test

الوصول الحر: https://doaj.org/article/f9849680ab0044ffac18341037683ecaTest

المؤلفون: Miaomiao Teng, Jiaqi Sun, Lihui Zhao, Yunxia Li, Zixuan Zhang, Wentao Zhu, Yuntao Zhang, Fangjingwei Xu, Sixi Xing, Xiaoli Zhao, Fengchang Wu

المصدر: Environment International, Vol 190, Iss , Pp 108851- (2024)

مصطلحات موضوعية: DEHP, DOTP, Vaccine, Gut microbiota, Short-chain fatty acids, Inflammation, Environmental sciences, GE1-350

الوصف: As the COVID-19 pandemic has progressed, increasing evidences suggest that the gut microbiota may play a crucial role in the effectiveness of SARS-CoV-2 vaccine. Thus, this study was aimed at investigating the influence of SARS-CoV-2 vaccine on the gut microbiota and short-chain fatty acids (SCFAs) of organisms exposed to environmental contaminants, i.e., plasticizers: phthalate esters. We found that in mice, exposure to dioctyl terephthalate (DOTP) and bis −2-ethylhexyl phthalate (DEHP) decreased the blood glucose level and white fat weight, induced inflammatory responses, caused damage to liver and intestinal tissues, and disrupted the gut microbiota composition and SCFAs metabolism. Specifically, the Bacteroidetes phylum was positively correlated with BBIBP-CorV vaccine, while acetic acid was negatively associated with the vaccine. Interestingly, the BBIBP-CorV vaccine somewhat alleviated tissue inflammation and reduced the contents of acetic acid and propionic acid in mice exposed to DEHP and DOTP. These findings were confirmed by a fecal microbiota transplantation assay. Overall, this study revealed that exposure to DEHP and DOTP adversely affects the gut microbiota and SCFAs, while the BBIBP-CorV vaccine can protect mice against these effects. This work highlighted the relationship between BBIBP-CorV vaccination, gut microbiome composition, and responses to plasticizers, which may facilitate the development and risk assessment of SARS-CoV-2 vaccines and environmental contaminants on microbiota health.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S0160412024004379Test; https://doaj.org/toc/0160-4120Test

الوصول الحر: https://doaj.org/article/9347db833a2a4e4fb05116903128ef4eTest