المؤلفون: Shuli Ma, Jie Ge, Lei Qin, Xiaoting Chen, Linlin Du, Yanbo Qi, Li Bai, Yunfeng Han, Zhiping Xie, Jiaxin Chen, Yuehui Jia

المصدر: JMIR Public Health and Surveillance, Vol 10, p e57807 (2024)

مصطلحات موضوعية: Public aspects of medicine, RA1-1270

الوصف: BackgroundThe World Health Organization declared mpox an international public health emergency. Since January 1, 2022, China has been ranked among the top 10 countries most affected by the mpox outbreak globally. However, there is a lack of spatial epidemiological studies on mpox, which are crucial for accurately mapping the spatial distribution and clustering of the disease. ObjectiveThis study aims to provide geographically accurate visual evidence to determine priority areas for mpox prevention and control. MethodsLocally confirmed mpox cases were collected between June and November 2023 from 31 provinces of mainland China excluding Taiwan, Macao, and Hong Kong. Spatiotemporal epidemiological analyses, including spatial autocorrelation and regression analyses, were conducted to identify the spatiotemporal characteristics and clustering patterns of mpox attack rate and its spatial relationship with sociodemographic and socioeconomic factors. ResultsFrom June to November 2023, a total of 1610 locally confirmed mpox cases were reported in 30 provinces in mainland China, resulting in an attack rate of 11.40 per 10 million people. Global spatial autocorrelation analysis showed that in July (Moran I=0.0938; P=.08), August (Moran I=0.1276; P=.08), and September (Moran I=0.0934; P=.07), the attack rates of mpox exhibited a clustered pattern and positive spatial autocorrelation. The Getis-Ord Gi* statistics identified hot spots of mpox attack rates in Beijing, Tianjin, Shanghai, Jiangsu, and Hainan. Beijing and Tianjin were consistent hot spots from June to October. No cold spots with low mpox attack rates were detected by the Getis-Ord Gi* statistics. Local Moran I statistics identified a high-high (HH) clustering of mpox attack rates in Guangdong, Beijing, and Tianjin. Guangdong province consistently exhibited HH clustering from June to November, while Beijing and Tianjin were identified as HH clusters from July to September. Low-low clusters were mainly located in Inner Mongolia, Xinjiang, Xizang, Qinghai, and Gansu. Ordinary least squares regression models showed that the cumulative mpox attack rates were significantly and positively associated with the proportion of the urban population (t0.05/2,1=2.4041 P=.02), per capita gross domestic product (t0.05/2,1=2.6955; P=.01), per capita disposable income (t0.05/2,1=2.8303; P=.008), per capita consumption expenditure (PCCE; t0.05/2,1=2.7452; P=.01), and PCCE for health care (t0.05/2,1=2.5924; P=.01). The geographically weighted regression models indicated a positive association and spatial heterogeneity between cumulative mpox attack rates and the proportion of the urban population, per capita gross domestic product, per capita disposable income, and PCCE, with high R2 values in north and northeast China. ConclusionsHot spots and HH clustering of mpox attack rates identified by local spatial autocorrelation analysis should be considered key areas for precision prevention and control of mpox. Specifically, Guangdong, Beijing, and Tianjin provinces should be prioritized for mpox prevention and control. These findings provide geographically precise and visualized evidence to assist in identifying key areas for targeted prevention and control.

وصف الملف: electronic resource

العلاقة: https://publichealth.jmir.org/2024/1/e57807Test; https://doaj.org/toc/2369-2960Test

الوصول الحر: https://doaj.org/article/eb214fcef50a4e9eb2b8dcf9cdd7c193Test

المؤلفون: Wei Mu, Meilan Zhang, Guang Hu, Yunfeng Han, Xia Mao, Caixia Chen, Kefeng Shen, Zhenyu Dai, Xiaojian Zhu, Xiaoxi Zhou, Liang Huang, Qilin Ao, Min Xiao

المصدر: Frontiers in Immunology, Vol 14 (2023)

مصطلحات موضوعية: ALK-positive anaplastic large cell lymphoma, cell therapy, chimeric antigen receptor T cells, CD5 antigen, laboratory based differential diagnosis, Immunologic diseases. Allergy, RC581-607

الوصف: BackgroundAnaplastic Large Cell Lymphoma (ALCL) is one of the most common subtypes of T-cell lymphoma. Among these, refractory and relapsed (r/r) ALK positive ALCL lacks effective therapies. The chimeric antigen receptor-modified T (CAR-T) cell therapy holds great promise as a therapeutic strategy for this disease. However, it is not known yet whether anti-CD5 CAR-T cells are sufficient for the definitive treatment of relapsed ALK+ ALCL, nor the role of accurate laboratory-based diagnoses during CAR-T treatment.Case presentationThe adolescent patient received autologous T cells containing sequences encoding VH domains specific to CD5. Following the infusion, there was an increase in both the copy number and proportion of CAR-T cells in peripheral blood. IL-6 and ferritin levels in the patient exhibited significant fluctuations, with increases of 13 and 70 folds respectively, compared to baseline after the treatment. Additionally, adverse effects were observed, including grade 4 rash, grade 1 headache, nausea, and neck-pain. Surprisingly, a relapsed disease phenotype was identified based on the results of PET/CT and histopathological analysis of the inguinal lymph node biopsy. After conducting a thorough diagnostic assessment, which included flow cytometry, next-generation sequencing (NGS), examination of immune-related gene rearrangements, and analysis of the immune repertoire of T-cell receptors (TCR), we conclusively determined that the hyperplastic T cells identified in the lymph node were the result of an expansion of CAR-T cells. Ultimately, the patient has attained complete remission (CR) and has sustained a disease-free survival state for 815 days as of the cutoff date on August 30, 2023.ConclusionTaken together, the results demonstrate that anti-CD5 CAR-T cells can induce a clinical response in r/r ALK+ ALCL patient. Furthermore, this case underscores the importance of utilizing advanced technologies with high sensitivity and accuracy for biological detection in clinical laboratory diagnosis and prognosis in CAR-T cell treatment.Trial registration numberNCT04767308.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fimmu.2023.1280007/fullTest; https://doaj.org/toc/1664-3224Test

الوصول الحر: https://doaj.org/article/09949907a8e54c0f90b2fdb13acd94d8Test

المؤلفون: Haiming Yu, Yunfeng Han, Rui Zhang, Chu Sun, Mingda Wang, Bo Yue, Kaiping Chou, Bin Li, Nan Zhang

المصدر: All Life, Vol 16, Iss 1 (2023)

مصطلحات موضوعية: lumbar spinal stenosis (lss), nerve root sedimentation sign (sedsign), difference cross-sectional area difference (csad), thickness of ligamentum flavum (tlf), grade of degenerative facet joint (dfj), Biotechnology, TP248.13-248.65, Life, QH501-531

الوصف: The incidence of nerve root sedimentation sign (SedSign) was evaluated to explore potential pathogenesis in patients with severe lumbar spinal stenosis (LSS). In a total of 209 patients with severe LSS, 290 intervertebral levels were narrow, among which 248 showed a positive SedSign, giving a prevalence of 85.52%. Those levels with a positive SedSign were further analyzed relative to those with a negative SedSign. There was no significant difference between the two groups for the cross-sectional area (CSA) or the posteroanterior diameter (PAD). In contrast, there was a significant difference between the groups for the grade of degenerative facet joint (DFJ) (p

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2689-5307Test

الوصول الحر: https://doaj.org/article/b886d148e23f4020817d88b9c51a4775Test

المؤلفون: Tan Xue, Chunfeng Li, Hongyan Zhang, Yunfeng Han, Jiahui Wu

المصدر: Hellenic Journal of Cardiology, Vol 63, Iss , Pp 32-39 (2022)

مصطلحات موضوعية: ASTER B, cholesterol, PKA, RhoA, myocardial damage, inflammatory factors, Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: Background: To investigate the effect of cholesterol accumulation in cells on the inflammatory process of acute myocardial infarction and cardiomyocytes and its mechanism. Methods: Blood samples of 15 patients with myocardial infarction were clinically collected to detect enzyme levels of cholesterol and related myocardial parameters in the serum. Correlation analysis was carried out. At the cellular level, simulation of cholesterol entry and exit from cells was conducted by a liposome-loaded cholesterol model in this study, and BNP and inflammatory factors were detected with enzyme-linked immunosorbent assay. Moreover, to investigate the molecular mechanism of myocardial damage caused by cholesterol, Gramd1b and Prkaca of HL-1 were knocked down with small interference RNA technique. Then, inhibitor C3 was used to weaken RhoA activity to explore the level of cardiac muscle cell BNP in order to identify key protein target sites that may be involved in the process of cholesterol damage to cardiac muscle cells. Results: Serum cholesterol concentration showed a significantly positive correlation with the levels of AST, CK, and LD in serum of patients with myocardial infarction. Cholesterol accumulation in cardiac muscle cells significantly increased the levels of BNP, inflammatory factors (IL-1β, IL-6, TNF-α, and CCL-2) in cardiac muscle cells, which exacerbated cardiomyocyte damage. Conversely, cholesterol excretion caused significant downregulation of BNP and inflammatory factors. Moreover, after knocking down Gramd1b, the accumulation of cholesterol in myocardial cells decreased, the levels of BNP and inflammatory factors significantly reduced, and the degree of myocardial cell damage was weakened. Knockdown of Prkaca inhibited RhoA activity and reversed cholesterol-induced elevation of BNP and inflammatory factors. Conclusion: ASTER B-mediated intracellular accumulation of cholesterol in cardiac muscle cells may cause cardiomyocyte damage and inflammatory factor infiltration through PKA-Ca2+-RhoA pathways.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S110996662100124XTest; https://doaj.org/toc/1109-9666Test

الوصول الحر: https://doaj.org/article/cd78ad260cc740e983857fbdf0425c98Test

المؤلفون: Wanzhong Yuan, Ran Huo, Kaiming Ma, Yunfeng Han, Xiaoliang Yin, Jun Yang, Xihai Zhao, Tao Wang

المصدر: Frontiers in Neurology, Vol 13 (2022)

مصطلحات موضوعية: carotid endarterectomy, severe carotid artery stenosis, contralateral carotid artery occlusion, shunt, carotid clamp time, Neurology. Diseases of the nervous system, RC346-429

الوصف: ObjectiveTo analyze the risk factors associated with adverse events after carotid endarterectomy (CEA) in patients with unilateral severe carotid stenosis and contralateral occlusion.MethodsPatients were recruited for CEA between August 2014 and February 2020. CEA was performed under general anesthesia. The carotid clamp time (CCT; long CCT: >20 min) is defined as the period between clamp-on and clamp-off for the stenotic carotid artery. The perioperative factors and postoperative adverse events were recorded. All patients were followed up for 1 year after CEA.ResultsSixty subjects (65.8 ± 7.2 years; 54 males) were included. Patients with adverse events had significantly longer CCT than those without adverse events (60% vs. 40%, P = 0.013). Univariate logistic regression analysis showed that a history of diabetes was significantly associated with adverse events (OR, 0.190; 95% CI, 0.045–0.814; P = 0.025); long CCT was significantly associated with adverse events (OR, 8.500; 95% CI, 1.617–44.682; P = 0.011). After adjusting for confounding factors, including age, sex, BMI, diabetes, PSV, long CCT, non–use of shunt, and history of stroke or TIA, the associations between diabetes and adverse events (OR, 0.113; 95% CI, 0.013–0.959; P = 0.046) were statistically significant; the associations between long CCT and adverse events (OR, 1.301; 95% CI, 1.049–1.613; P = 0.017) were statistically significant.ConclusionsA longer carotid clamp time (>20 min) and a history of diabetes may increase the risk of adverse events in patients with unilateral severe carotid stenosis and contralateral occlusion after CEA. With good preoperative evaluation and intraoperative monitoring, the use of shunts may not be needed intraoperatively in patients with unilateral severe carotid stenosis and contralateral occlusion.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fneur.2022.971673/fullTest; https://doaj.org/toc/1664-2295Test

الوصول الحر: https://doaj.org/article/72a9633d1fd346c5820c42055915780fTest

المؤلفون: Yichun Cheng, Liu Huang, Yunfeng Han, Chummun Vanisha, Shuwang Ge, Gang Xu

المصدر: BMC Cancer, Vol 20, Iss 1, Pp 1-9 (2020)

مصطلحات موضوعية: Estimated glomerular filtration rate, Measured glomerular filtration rate, MDRD formula, Nomogram, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract Background Formulae of estimated glomerular filtration rate (eGFR) based on serum creatinine (Scr) are routinely used in oncology patients, however, they are inaccurate in some populations. Our aim was to assess the agreement of eGFR formulae and thereby build a nomogram to predict the reliability of estimates. Methods Measured GFR (mGFR) using isotope from 445 oncology patients were compared with eGFR from six formulae (Cockcroft-Gault, Modification of Diet in Renal Disease (MDRD), modified MDRD formulae for Chinese (C-MDRD), Chronic Kidney Disease Epidemiology (CKD-EPI) Collaboration, Wright and full age spectrum (FAS)). Bias, precision and accuracy of eGFR formulae were examined. We also evaluated statistics of agreement: the total deviation index (TDI), the concordance correlation coefficient (CCC) and the coverage probability (CP). Multivariate logistic regression was applied to identify characteristics associated with inaccurate eGFR and construct a predictive nomogram. Results All eGFR formulae tended to overestimate the eGFR. The percentage of patients with eGFR within 30% the mGFR ranged from 38.0 to 62.8%. Cockcroft-Gault and MDRD showed low bias and high precision. The MDRD formula exhibited lowest TDI, meaning that 90% of estimations ranged from − 36 to 36% of mGFR. Multivariate logistic regression showed that inaccuracy of MDRD was found in elderly patients or in patients with eGFR greater than 120 ml/min. A nomogram was constructed to help oncologists to predict the risk of inaccuracy of eGFR. The calibration curve showed good agreement. Conclusions Our results suggest that the error of eGFR by any formulae was common and wide in Chinese oncology patients. Our nomogram may assist oncologists in decision-making when mGFR is needed.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s12885-020-06997-wTest; https://doaj.org/toc/1471-2407Test

الوصول الحر: https://doaj.org/article/1dbc283fbefa45209cbbde0fd7717a2cTest

المؤلفون: Yuehui Jia, Yanbo Qi, Li Bai, Yunfeng Han, Zhiping Xie, Jie Ge

المصدر: BMJ Open, Vol 11, Iss 2 (2021)

مصطلحات موضوعية: Medicine

الوصف: Objectives This study aimed to investigate the knowledge–attitude–practice (KAP) of Chinese college students regarding COVID-19 and evaluate their psychological status against the background of the COVID-19 outbreak.Design This was a cross-sectional study.Setting This study covered 31 provinces, municipalities and autonomous regions of mainland China.Participants The participants, who were college students with ordinary full-time status, were surveyed anonymously on their KAP regarding COVID-19 by using self-made questionnaires. In addition, the Self-Rating Anxiety Scale was used to assess the psychological status of the students.Methods The online cross-sectional study among Chinese college students was conducted in February 2020. Logistic regression analysis was used to analyse the predictors of anxiety symptoms.Primary outcome measures The level of KAP and anxiety symptoms.Results A total of 740 college students from 31 provinces, municipalities and autonomous regions in China were recruited in the survey. Among them, 139 (18.78%) revealed having anxiety. Multivariable logistic regression analysis revealed that female gender was the risk factor for anxiety symptoms with an increased 2.164-fold risk than male gender (OR=2.164, 95% CI=1.279 to 3.662). The knowledge (OR=0.825, 95% CI=0.779 to 0.873) and attitude (OR=0.822, 95% CI=0.762 to 0.887) regarding COVID-19 were protective factors against anxiety symptoms.Conclusions The level of KAP regarding COVID-19 was significantly negatively correlated with anxiety symptoms. Thus, understanding the level of KAP among college students during the early stages of major public health emergencies, such as a pandemic, is important. Such understanding plays an important role in adopting targeted health education strategies and reducing the psychological damage caused by these emergencies.

وصف الملف: electronic resource

العلاقة: https://bmjopen.bmj.com/content/11/2/e045034.fullTest; https://doaj.org/toc/2044-6055Test

الوصول الحر: https://doaj.org/article/a61e3ad5ae114ab0a0b479ec865c00adTest

المؤلفون: Menghao Li, Yang Liu, Yanxiong Liu, Guanxu Chen, Qiuhua Tang, Yunfeng Han, Yuanlan Wen

المصدر: Remote Sensing, Vol 14, Iss 8, p 1939 (2022)

مصطلحات موضوعية: GNSS-acoustic, underwater geodetic network, mixed network configuration, position dilution of precision, acoustic transmission characteristics, Science

الوصف: The construction of underwater geodetic networks (UGN) is crucial in marine geodesy. To provide high-precision kinematic positioning for underwater submersibles, an underwater acoustic geodetic network configuration of three seafloor base stations, one subsurface buoy, and one sea surface buoy is proposed. The simulation results show that, for a 3 km-deep sea, based on the proposed UGN, the submersible positioning range and positioning accuracy are primarily affected by the size of the seafloor base station array, while the height of the subsurface buoy has a greater impact on the submersible positioning accuracy than the positioning range. Considering current acoustic ranging technology, the kinematic positioning performance of the UGN is optimal when the seafloor base stations are 9~13 km apart and the subsurface buoy is less than 2.5 km above the seafloor, which can achieve a submersible positioning accuracy of less than 30 m within an underwater space of 25 km × 25 km × 3 km. The proposed cost-effective UGN configuration can provide high-precision submersible kinematic positioning performance for seafloor surveying and ocean precision engineering. The impact of the underwater environment on the acoustic transmission characteristics should be further investigated.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2072-4292/14/8/1939Test; https://doaj.org/toc/2072-4292Test

الوصول الحر: https://doaj.org/article/d8053c3c404a4894ab3c63e18a32c136Test

المؤلفون: Jiali Li, Yang Xun, Cong Li, Yunfeng Han, Yaqi Shen, Xuemei Hu, Daoyu Hu, Zheng Liu, Shaogang Wang, Zhen Li

المصدر: Frontiers in Medicine, Vol 7 (2020)

مصطلحات موضوعية: unenhanced CT, differential renal function, residual parenchymal volume, CT texture analysis, upper urinary tract stones, Medicine (General), R5-920

الوصف: Objectives: The aim of this study was to determine whether unenhanced computed tomography (CT) imaging can estimate differential renal function (DRF) in patients with chronic unilateral obstructive upper urinary tract stones.Materials and Methods: This was a single-center retrospective study of 76 patients. All the patients underwent unenhanced CT and nuclear renography (RG) at an interval of 4 to 6 weeks due to chronic unilateral obstructive urinary stones. Renal CT measurements (RCMs), including residual parenchymal volume (RPV) and volumetric CT texture analysis parameters, were obtained through a semiautomatic method. Percent RCMs were calculated and compared with renal function determined by RG.Results: The strongest Pearson coefficient between percent RCM and DRF was reflected by RPV (r = 0.957, P < 0.001). Combinations of RPV and other parameters did not significantly improve the correlation compared with RPV alone (r = 0.957 vs. r = 0.957, 0.957, 0.887, 0.815, and 0.956 for combination with Hounsfield unit, parenchymal voxel, skewness, kurtosis, and entropy, respectively; all P < 0.001). Percent RPV was subsequently introduced into linear regression, and the equation y = −2.66 + 1.07* × (P < 0.001) was derived to calculate predicted DRF. No statistically difference was found between predicted DRF using the equation and observed DRF according to RG (P = 0.959).Conclusion: Unenhanced CT imaging can estimate DRF in patients with chronic unilateral obstructive upper urinary tract stones, and RG might not be necessary as a conventional method in clinical.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/article/10.3389/fmed.2020.00309/fullTest; https://doaj.org/toc/2296-858XTest

الوصول الحر: https://doaj.org/article/c13ef9264d574390b0bfc19c3490d6fdTest

المؤلفون: Yunfeng Han, Xuan Lai, Xiaodong Chen, Jingcheng Xie, Fang Mei, Ming Zhang, Jianjun Sun, Zhenyu Wang, Jun Yang

المصدر: Interdisciplinary Neurosurgery, Vol 18, Iss , Pp - (2019)

مصطلحات موضوعية: Surgery, RD1-811, Neurology. Diseases of the nervous system, RC346-429

الوصف: Introduction: Dumbbell schwannoma complicated by intradural lumbar disc herniation at the same level is extremely rare. Case presentation: We describe a 67-year-old female presented with low back pain for 10 years and weakness and numbness of the right lower extremity for one month. Contrast magnetic resonance imaging (MRI) revealed an inhomogeneous enhanced dumbbell lesion at the L4/5 right intervertebral foramen and the intradural part of the lesion is only annular enhanced. A single posterior-approach surgery, including L4 laminectomy and right L4/5 facet joint resection, intracapsular resection of extradural dumbbell tumor, and intradural nucleus pulposus resection, was performed. The patient's back pain and weakness of right lower extremity disappeared. Histological studies revealed a schwannoma and a herniated disc. Conclusions: To our knowledge, this case is the first report of dumbbell schwannoma complicated by intradural lumbar disc herniation. Contrast MRI is helpful in preoperative differential diagnosis. Careful preoperative images reading are important to prevent misdiagnosis. Keywords: Dumbbell schwannoma, Intradural disc herniation, Resective surgery

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2214751919300556Test; https://doaj.org/toc/2214-7519Test

الوصول الحر: https://doaj.org/article/4497585e6c27477fae99f2e61c961b80Test