المؤلفون: Shoya Fukatsu, Yuki Miyamoto, Yu Oka, Maki Ishibashi, Remina Shirai, Yuki Ishida, Shin Endo, Hironori Katoh, Junji Yamauchi

المصدر: Neurology International, Vol 16, Iss 1, Pp 33-61 (2023)

مصطلحات موضوعية: monomeric GTP-binding proteins, phosphotransferases, signal transduction, disease, flavonoid, RNA, Medicine, Internal medicine, RC31-1245, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571

الوصف: Recent discoveries suggest links between abnormalities in cell morphogenesis in the brain and the functional deficiency of molecules controlling signal transduction in glial cells such as oligodendroglia. Rnd2 is one such molecule and one of the Rho family monomeric GTP-binding proteins. Despite the currently known functions of Rnd2, its precise roles as it relates to cell morphogenesis and disease state remain to be elucidated. First, we showed that signaling through the loss of function of the rnd2 gene affected the regulation of oligodendroglial cell-like morphological differentiation using the FBD-102b cell line, which is often utilized as a differentiation model. The knockdown of Rnd2 using the clustered regularly interspaced palindromic repeats (CRISPR)/CasRx system or RNA interference was shown to slow morphological differentiation. Second, the knockdown of Prag1 or Fyn kinase, a signaling molecule acting downstream of Rnd2, slowed differentiation. Rnd2 or Prag1 knockdown also decreased Fyn phosphorylation, which is critical for its activation and for oligodendroglial cell differentiation and myelination. Of note, hesperetin, a citrus flavonoid with protective effects on oligodendroglial cells and neurons, can recover differentiation states induced by the knockdown of Rnd2/Prag1/Fyn. Here, we showed that signaling through Rnd2/Prag1/Fyn is involved in the regulation of oligodendroglial cell-like morphological differentiation. The effects of knocking down the signaling cascade molecule can be recovered by hesperetin, highlighting an important molecular structure involved in morphological differentiation.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2035-8377/16/1/3Test; https://doaj.org/toc/2035-8377Test

الوصول الحر: https://doaj.org/article/f1d0953ed0044ceabf31fadc9f458894Test

المؤلفون: Takamasa Homma, Kuniaki Tanaka, Naoya Takeda, Yu Okada, Shota Torii, Hideki Esaki, Takashi Sakakibara, Norio Takimoto

المصدر: Case Reports in Oncology, Vol 16, Iss 1, Pp 1217-1222 (2023)

مصطلحات موضوعية: enfortumab vedotin, urothelial carcinoma, asthma, nectin-4, antibody-drug conjugate, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Enfortumab vedotin is an antibody-drug conjugate against nectin-4 that is recently being used in the management of patients with urothelial carcinoma. The common adverse events include rashes, peripheral neuropathy, and hyperglycemia. Only a few cases of associated respiratory symptoms have been reported. Herein, we describe 2 patients with advanced urothelial carcinoma who experienced asthma exacerbation after initiating enfortumab vedotin treatment. Both patients improved with inhalation therapy. Since nectin-4 is expressed in the tracheal epithelium, its association with asthma is likely. This study highlights that clinicians should caution patients with a history of asthma against the worsening of respiratory symptoms when enfortumab vedotin is administered.

وصف الملف: electronic resource

العلاقة: https://beta.karger.com/Article/FullText/534150Test; https://doaj.org/toc/1662-6575Test

الوصول الحر: https://doaj.org/article/2f1555a2f1604a6394b0ee9b59018b16Test

المؤلفون: Hidetoshi Minamida, Ichitaka Kimura, Norihito Sasaki, Yu Okamoto, 佐々木 紀仁, 南田 英俊, 岡本 悠, 木村 一隆

المصدر: 日本プライマリ・ケア連合学会誌 / An Official Journal of the Japan Primary Care Association. 2023, 46(4):157

المؤلفون: Yu Okazawa, Kiichi Sugimoto, Yuki Ii, Takahiro Irie, Megumi Kawaguchi, Aya Kobari, Hirotaka Momose, Yuki Tsuchiya, Kota Amemiya, Shunsuke Motegi, Ryoichi Tsukamoto, Kazumasa Kure, Kumpei Honjo, Hisashi Ro, Rina Takahashi, Shingo Kawano, Masaya Kawai, Shun Ishiyama, Makoto Takahashi, Yutaka Kojima, Yuichi Tomiki, Atsushi Arakawa, Takashi Yao, Kaishi Satomi, Yuko Matsushita, Koichi Ichimura, Kazuhiro Sakamoto

المصدر: DEN Open, Vol 3, Iss 1, Pp n/a-n/a (2023)

مصطلحات موضوعية: cecal cancer, copy number variation, endoscopic submucosal dissection, local recurrence, submucosal invasive colorectal cancer, Diseases of the digestive system. Gastroenterology, RC799-869

الوصف: Abstract We report a case in which analysis of copy number variation revealed local recurrence of submucosal invasive colorectal cancer after curative endoscopic submucosal dissection (ESD). An 86‐year‐old man with a history of abdominoperineal resection of the rectum for rectal cancer underwent resection with ESD for early‐stage sigmoid cancer 5 cm away from the stoma opening. At the same time, ileocecal resection was performed for advanced cecal cancer. Twelve months after ESD, advanced cancer occurred in the area of the ESD lesion. It was unclear if the cancer was a local recurrence after ESD, implantation of cecal cancer, or a new lesion. Copy number variation analysis performed for the three lesions revealed that the new lesion originated from residual tumor cells from ESD and was unlikely to be cecal cancer.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2692-4609Test

الوصول الحر: https://doaj.org/article/b92575499463489a817f23f3dc0032a7Test

المؤلفون: AYU KATAYAMA, GOH NISHITANI, IAN G. GLEADALL, KEIJI MATSUBARA, MASAMI ABE, MASAZUMI NISHIKAWA, NOBUHIKO AKIYAMA, RIKU KIMURA, SHUNZO MORII, YU OKAWARA, YUTA SUZUMURA, イアン グレドル, 大河原 遊, 木村 理久, 松原 圭史, 森井 俊三, 片山 亜優, 秋山 信彦, 西川 正純, 西谷 豪, 鈴村 優太, 阿部 正美

المصدر: 日本水産学会誌 / NIPPON SUISAN GAKKAISHI. 2023, 89(4):304

المؤلفون: Yu Okae, Kohei Nishitani, Akio Sakamoto, Toshiyuki Kawai, Takuya Tomizawa, Motoo Saito, Yutaka Kuroda, Shuichi Matsuda

المصدر: Frontiers in Cellular and Infection Microbiology, Vol 12 (2022)

مصطلحات موضوعية: antibiotics, biofilm, infection, implant, Staphylococcus aureus - bacteria, Microbiology, QR1-502

الوصف: The formation of a biofilm on the implant surface is a major cause of intractable implant-associated infection. To investigate the antibiotic concentration needed to eradicate the bacteria inside a biofilm, the minimum biofilm eradication concentration (MBEC) has been used, mostly against in vitro biofilms on plastic surfaces. To produce a more clinically relevant environment, an MBEC assay against biofilms on stainless-steel implants formed in a rat femoral infection model was developed. The rats were implanted with stainless steel screws contaminated by two Staphylococcus aureus strains (UAMS-1, methicillin-sensitive Staphylococcus aureus; USA300LAC, methicillin-resistant Staphylococcus aureus) and euthanized on days 3 and 14. Implants were harvested, washed, and incubated with various concentrations (64–4096 μg/mL) of gentamicin (GM), vancomycin (VA), or cefazolin (CZ) with or without an accompanying systemic treatment dose of VA (20 μg/mL) or rifampicin (RF) (1.5 μg/mL) for 24 h. The implant was vortexed and sonicated, the biofilm was removed, and the implant was re-incubated to determine bacterial recovery. MBEC on the removed biofilm and implant was defined as in vivo MBEC and in vivo implant MBEC, respectively, and the concentrations of 100% and 60% eradication were defined as MBEC100 and MBEC60, respectively. As for in vivo MBEC, MBEC100 of GM was 256–1024 μg/mL, but that of VA and CZ ranged from 2048–4096 μg/mL. Surprisingly, the in vivo implant MBEC was much higher, ranging from 2048 μg/mL to more than 4096 μg/mL. The addition of RF, not VA, as a secondary antibiotic was effective, and MBEC60 on day 3 USA300LAC biofilm was reduced from 1024 μg/mL with GM alone to 128 μg/mL in combination with RF and the MBEC60 on day 14 USA300LAC biofilm was reduced from 2048 μg/mL in GM alone to 256 μg/mL in combination with RF. In conclusion, a novel MBEC assay for in vivo biofilms on orthopedic implants was developed. GM was the most effective against both methicillin-sensitive and methicillin-resistant Staphylococcus aureus, in in vivo biofilms, and the addition of a systemic concentration of RF reduced MBEC of GM. Early initiation of treatment is desired because the required concentration of antibiotics increases with biofilm maturation.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fcimb.2022.896978/fullTest; https://doaj.org/toc/2235-2988Test

الوصول الحر: https://doaj.org/article/0cecf104735849d3b02dbaca80c6abb2Test

المؤلفون: Yuki Tsuchiya, Hirotaka Momose, Kazumasa Kure, Hisashi Ro, Rina Takahashi, Yu Okazawa, Masaya Kawai, Makoto Takahashi, Yutaka Kojima, Kazuhiro Sakamoto

المصدر: Case Reports in Gastroenterology, Vol 15, Iss 1, Pp 35-40 (2021)

مصطلحات موضوعية: groin hernia, incarcerated femoral hernia, laparoscopic surgery, Diseases of the digestive system. Gastroenterology, RC799-869

الوصف: An 81-year-old man was brought to our hospital due to a suspicion of left incarcerated femoral hernia. He was previously diagnosed with incarcerated left groin hernia and was treated using the mesh plug method 1 month back at another hospital. Abdominal computed tomography scan revealed small bowel obstruction, incarcerated bowel, and compression of the left femoral vein. Thus, the patient was diagnosed with incarcerated femoral hernia. An emergency laparoscopic surgery was then performed, and we found that the small bowel was incarcerated into the let femoral ring and was necrotic. However, there was no recurrence of left inguinal hernia. The small necrotic bowel was resected and the femoral ring was repaired. The patient was discharged 8 days after the surgery, and there was no recurrence of femoral hernia after 1 year.

وصف الملف: electronic resource

العلاقة: https://www.karger.com/Article/FullText/509950Test; https://doaj.org/toc/1662-0631Test

الوصول الحر: https://doaj.org/article/1bbce36c1d9545f68d933675cfd7f67cTest

المؤلفون: Takamitsu Hori, Daisuke Mizutani, Takashi Onuma, Yu Okada, Kumi Kojima, Tomoaki Doi, Yukiko Enomoto, Hiroki Iida, Shinji Ogura, Takashi Sakurai, Toru Iwama, Osamu Kozawa, Haruhiko Tokuda

المصدر: International Journal of Molecular Sciences, Vol 23, Iss 22, p 14100 (2022)

مصطلحات موضوعية: Amyloid β protein, platelet, thrombin receptor-activating protein, platelet-derived growth factor, diabetes mellitus, brain atrophy, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: Type 2 DM is a risk factor for dementia, including Alzheimer’s disease (AD), and is associated with brain atrophy. Amyloid β protein (Aβ) deposition in the brain parenchyma is implicated in the neurodegeneration that occurs in AD. Platelets, known as abundant storage of Aβ, are recognized to play important roles in the onset and progression of AD. We recently showed that Aβ negatively regulates platelet activation induced by thrombin receptor-activating protein (TRAP) in healthy people. In the present study, we investigated the effects of Aβ on the TRAP-stimulated platelet activation in DM patients, and the relationship between the individual responsiveness to Aβ and quantitative findings of MRI, the volume of white matter hyperintensity (WMH)/intracranial volume (IC) and the volume of parenchyma (PAR)/IC. In some DM patients, Aβ reduced platelet aggregation induced by TRAP, while in others it was unchanged or rather enhanced. The TRAP-induced levels of phosphorylated-Akt and phosphorylated-HSP27, the levels of PDGF-AB and the released phosphorylated-HSP27 correlated with the degree of platelet aggregability. The individual levels of not WMH/IC but PAR/IC was correlated with those of TRAP-stimulated PDGF-AB release. Collectively, our results suggest that the reactivity of TRAP-stimulated platelet activation to Aβ differs in DM patients from healthy people. The anti-suppressive feature of platelet activation to Aβ might be protective for brain atrophy in DM patients.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1422-0067/23/22/14100Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/b062839398a24999a858e63e67f7c8daTest

المؤلفون: Atsumu Terada, Fumihiro Murakami, Jongmyung Park, Ken Matsukuma, Teruyuki Yoshimitsu, Toru Sugiyama, Yu Okamura, 吉満 輝行, 寺田 貴武, 岡村 優, 朴 鐘明, 杉山 徹, 村上 文洋, 松隈 健

المصدر: 日本婦人科腫瘍学会雑誌 / Japanese Journal of Gynecological Oncology. 2023, 41(1):1

المؤلفون: Hirotaka Momose, Kazuhiro Sakamoto, Kiichi Sugimoto, Makoto Takahashi, Masaya Kawai, Megumi Kawaguchi, Rina Takahashi, Ryoichi Tsukamoto, Shunsuke Motegi, Yu Okazawa, Yuki Ii, Yutaka Kojima, 井 祐樹, 坂本 一博, 塚本 亮一, 小島 豊, 岡澤 裕, 杉本 起一, 河口 恵, 河合 雅也, 百瀬 裕隆, 茂木 俊介, 高橋 玄, 高橋 里奈

المصدر: 日本大腸肛門病学会雑誌 / Nippon Daicho Komonbyo Gakkai Zasshi. 2023, 76(3):299