المؤلفون: Yi-Hao Chiang, Ching-Ting Lien, Wen-Hao Su, Tsung-Yu Yen, Yu-Jen Chen, Yuen-Liang Lai, Ken-Hong Lim, Kun-Yao Dai, Hsin-Pei Chung, Chia-Yen Hung, Yi-Shing Leu

المصدر: BMC Palliative Care, Vol 23, Iss 1, Pp 1-9 (2024)

مصطلحات موضوعية: Fentanyl buccal soluble film, FBSF, Breakthrough cancer pain, Around-the‐clock, Rapid-onset opioids, Proportional, Special situations and conditions, RC952-1245

الوصف: Abstract Background Clinical evidence for the rapidity and effectiveness of fentanyl buccal soluble film (FBSF) in reducing pain intensity of breakthrough cancer pain (BTcP) remains inadequate. This study aimed to evaluate the efficacy of FBSF proportional to the around-the‐clock (ATC) opioid regimens in rapidly relieving the intensity of BTcP episodes by determining the percentage of patients requiring further dose titration. Methods The study procedure included a dose-finding period followed by a 14-day observation period. Pain intensity was recorded with a Numeric Rating Scale (NRS) at onset and 5, 10, 15, and 30 min after FBSF self-administration. Meaningful pain relief was defined as the final NRS score ≤ 3. Satisfaction survey was conducted for each patient after treatment using the Global Satisfaction Scale. Results A total of 63 BTcP episodes occurred in 30 cancer patients. Only one patient required rescue medication at first BTcP episode and then achieved meaningful pain relief after titrating FBSF by 200 µg. Most BTcP episodes relieved within 10 min. Of 63 BTcP episodes, 30 (47.6%), 46 (73.0%), and 53 (84.1%) relieved within 5, 10, and 15 min after FBSF administration. Only grade 1/2 adverse events were reported, including somnolence, malaise, and dizziness. Of the 63 BTcP episodes, 82.6% were rated as excellent/good satisfaction with FBSF. Conclusion FBSF can be administrated “on demand” by cancer patients at the onset of BTcP, providing rapid analgesia by achieving meaningful pain relief within 10 min. Trial registration This study was retrospectively registered 24 December, 2021 at Clinicaltrial.gov (NCT05209906): https://clinicaltrials.gov/study/NCT05209906Test .

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1472-684XTest

الوصول الحر: https://doaj.org/article/990397aded314904b25b731d8795187eTest

المؤلفون: James Mei-Lin Chan, Yuan-Ching Chang, Hua-Chen Chan, Hsiu-Chuan Chan, Wei-Chin Chang, Liu-Fang Wang, Tung-Hu Tsai, Yu-Jen Chen, Wen-Chien Huang

المصدر: Molecular Medicine, Vol 30, Iss 1, Pp 1-14 (2024)

مصطلحات موضوعية: Human malignant pleural mesothelioma (hMPM), Epithelioid, Sarcomatoid, Non-epithelioid, Romidepsin (FK228), Therapeutics. Pharmacology, RM1-950, Biochemistry, QD415-436

الوصف: Abstract Human malignant pleural mesothelioma (hMPM) is an aggressive, rare disease with a poor prognosis. Histologically, MPM is categorized into epithelioid, biphasic, and sarcomatoid subtypes, with the epithelioid subtype generally displaying a better response to treatment. Conversely, effective therapies for the non-epithelioid subtypes are limited. This study aimed to investigate the potential role of FK228, a histone deacetylase inhibitor, in the suppression of hMPM tumor growth. We conducted a comprehensive analysis of the histological and molecular characteristics of two MPM cell lines, CRL-5820 (epithelioid) and CRL-5946 (non-epithelioid). CRL-5946 cells and non-epithelioid patient-derived xenografted mice exhibited heightened growth rates compared to those with epithelioid MPM. Both CRL-5946 cells and non-epithelioid mice displayed a poor response to cisplatin. However, FK228 markedly inhibited the growth of both epithelioid and non-epithelioid tumor cells in vitro and in vivo. Cell cycle analysis revealed FK228-induced G1/S and mitotic arrest in MPM cells. Caspase inhibitor experiments demonstrated that FK228-triggered apoptosis occurred via a caspase-dependent pathway in CRL-5946 but not in CRL-5820 cells. Additionally, a cytokine array analysis showed that FK228 reduced the release of growth factors, including platelet-derived and vascular endothelial growth factors, specifically in CRL-5946 cells. These results indicate that FK228 exhibits therapeutic potential in MPM by inducing cytotoxicity and modulating the tumor microenvironment, potentially benefiting both epithelioid and non-epithelioid subtypes.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1528-3658Test

الوصول الحر: https://doaj.org/article/3de7829d7ba14934a8a64cf707823b20Test

المؤلفون: Yu-Chuan Lin, Yu-Jen Chen, Tsang-Yu Fan, Pei-Hsi Chou, Cheng-Chang Lu

المصدر: Journal of Orthopaedic Surgery and Research, Vol 19, Iss 1, Pp 1-12 (2024)

مصطلحات موضوعية: Graft healing, Graft maturation, Laxity, MRI, Orthopedic surgery, RD701-811, Diseases of the musculoskeletal system, RC925-935

الوصف: Abstract Background The effect of bone marrow aspirate concentrate (BMAC) and platelet-rich plasma (PRP) combination in enhancing graft maturation and tendon–bone tunnel interfacial healing after anterior cruciate ligament (ACL) reconstruction remains unclear. We hypothesised that BMAC and PRP combination could lead to better clinical results and better graft maturation/interface healing than PRP alone or conventional ACL reconstruction without any other biologic augmentation. Methods In this randomised double-blind prospective study, patients undergoing ACL reconstruction surgery were randomly assigned into three groups: (1) control group (without any biologic augmentation), (2) PRP treatment group, and (3) combined BMAC and PRP (BMAC + PRP) group. Moreover, they were evaluated using the clinical functional score, laxity examination, and magnetic resonance imaging (MRI) analysis. Results No significant difference was observed in the improvement of functional scores among groups. However, laxity improvement at 24 weeks showed a significant difference with the BMAC + PRP group having the lowest laxity. MRI analysis showed no significant change in whole graft maturation among groups. In particular, the BMAC + PRP group showed delayed signal peak and higher graft signal at 24 weeks compared with the other two groups; however, the difference was not significant. With regard to tendon–bone interfacial healing, the BMAC + PRP group showed significantly wider tendon–bone interface in the femoral bone tunnel at 24 weeks compared with the other two groups. Moreover, the BMAC + PRP group showed significantly higher peri-tunnel edema signal in the femoral bone tunnel at 12 weeks compared with the other two groups. Conclusion PRP alone and BMAC and PRP combination showed limited enhancing effect in clinical function, graft maturation and tendon–bone interfacial healing compared with control (no additional treatment). When BMAC is used in ACL reconstruction, the possibility of greater inflammation in the early stage to graft maturation and bone tunnel healing should be considered.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1749-799XTest

الوصول الحر: https://doaj.org/article/5eae9dc15f4946dd856a0bf393586eb3Test

المؤلفون: Jie Lee, Jhen-Bin Lin, Chia-Sui Weng, Sue-Jar Chen, Tze-Chien Chen, Yu-Jen Chen

المصدر: Clinical and Translational Radiation Oncology, Vol 43, Iss , Pp 100671- (2023)

مصطلحات موضوعية: Reduced margin, Pelvic radiotherapy, Image-guided radiotherapy, Gynecological cancer, Gastrointestinal toxicity, Optimized outcome, Medical physics. Medical radiology. Nuclear medicine, R895-920, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Purpose: To investigate the effect of reduced margin pelvic radiotherapy on gastrointestinal toxicity and outcomes in gynecological cancer. Materials and methods: This retrospective study analyzed data of 590 patients who underwent hysterectomy and adjuvant pelvic radiotherapy between 2010 and 2020 at two tertiary centers. The pelvic nodal region was delineated based on a reduced margin definition or the Radiation Therapy Oncology Group (RTOG) guidelines. All patients were treated with intensity-modulated radiotherapy with imaging guidance. Gastrointestinal toxicity was assessed using the Common Terminology Criteria for Adverse Events (CTCAE) and the Patient-Reported Outcome version (PRO-CTCAE). Results: Overall, 352 (59.7%) and 238 (40.3%) patients underwent RTOG and reduced margin pelvic radiotherapy, respectively. Median follow-up was 6.4 years (IQR: 3.7–9.6). Reduced margin pelvic radiotherapy significantly lowered the radiation dose to the small bowel. For CTCAE grade ≥ 2 or 3, acute gastrointestinal toxicity was lower in the reduced margin group than in the RTOG group (16.4% vs. 33.5%, p

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405630823000964Test; https://doaj.org/toc/2405-6308Test

الوصول الحر: https://doaj.org/article/d5f15bf69d78498ab179a0eef8975048Test

المؤلفون: Yu-Jen Chen, Ming-Chih Hou, Tsung-Chieh Yang, Pei-Chang Lee, Yi-Hsiang Huang, Fa-Yauh Lee

المصدر: BMC Gastroenterology, Vol 23, Iss 1, Pp 1-9 (2023)

مصطلحات موضوعية: COVID-19, Esophageal varices, Triage policy, Diseases of the digestive system. Gastroenterology, RC799-869

الوصف: Abstract Background & aims During the COVID-19 pandemic, most of the endoscopic services were electively postponed or suspended. We aimed to assess the safety of a triage policy in patients receiving esophageal variceal ligation during the COVID-19 pandemic. Methods Triage policy of endoscopic variceal ligation (EVL) was implemented in our hospital during the lockdown period from 15th May 2021 to 26th July 2021. One experienced gastroenterologist reviewed the prior-scheduled list of patients for the EVL prophylaxisprogram. We compared the clinical characteristics and outcomes with those receiving endoscopy due to esophageal varices from 17th May 2020 to 28th July 2020. Results Of the 124 patients receiving EVL, a higher percentage of esophageal variceal bleeding (EVB) was noted (9/32, 28.1% vs. 8/92, 8.7%, p = 0.006) during the lockdown period, with a higher percentage of EVB in the referrals (7/9, 77.8% vs. 2/14, 14.2%, p = 0.007). Among patients who received prophylactic EVL, 6 of 78 (7.7%) experienced EVB during the normal period, which is no different to 2 of 23 (8.7%) during the lockdown period. Twenty-three patients whose endoscopies were postponed by triage policy due to low-risk or eradicated varices did not experience EVB during the lockdown period. Child-Turcotte-Pugh (CTP) class C was predictive of EVB (relative risk 8.400, P = 0.033), entering the program of prophylactic EVL was the protective factor of EVB (relative risk 0.016, P = 0.002). Conclusion Entrance into the prophylaxis program does not only decreases risk of EVB but also fosters comprehensive triage to postpone endoscopy during the lockdown period.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-230XTest

الوصول الحر: https://doaj.org/article/9b65e6f6281840b49201a3a7103705b6Test

المؤلفون: Yu-Jen Chen, Ming-Chih Hou, Tsung-Chieh Yang, Pei-Chang Lee, Yen-Po Wang, Yi-Hsiang Huang, Fa-Yauh Lee

المصدر: BMC Gastroenterology, Vol 23, Iss 1, Pp 1-8 (2023)

مصطلحات موضوعية: Percutaneous endoscopic gastrostomy (PEG), Peristomal wound infection, Povidone-iodine, Diseases of the digestive system. Gastroenterology, RC799-869

الوصف: Abstract Background Peristomal wound infection is a common complication in patients receiving percutaneous endoscopic gastrostomy (PEG). The main reason for peristomal infection might be the oral microbes coating the gastrostomy tube during implantation. Povidone-iodine solution can be applied for skin and oral decontamination. We designed a randomized controlled trial to test the effectiveness of a Betadine® (povidone-iodine) coated gastrostomy tube to reduce peristomal infection after percutaneous endoscopic gastrostomy. Methods A total of 50 patients were randomized to Betadine and control groups (25 patients in each group) from April 2014 to August 2021 at a tertiary medical center. All patients received the pull method for PEG implantation using a 24-french gastrostomy tube. The primary endpoint was peristomal wound infection rate 2 weeks after the procedure. Results Changes in Neutrophil/Lymphocyte ratio (N/L ratio) and C-Reative protein (Delta CRP) at 24 h after PEG were higher in the control group than in the Betadine group (N/L ratio, 3.1 vs. 1.2, p = 0.047; CRP, 2.68 vs.1.16, p = 0.009). The two groups did not differ in post-PEG fever, peristomal infection, pneumonia, or all-cause infection. Delta CRP could predict peristomal infection and all-cause infection within 2 weeks (AUROC 0.712 vs. 0.748; p = 0.039 vs. 0.008). The best cut-off-point of Delta CRP for the diagnosis of peristomal wound infection was 3 mg/dl. Conclusion The betadine coating gastrostomy tube method could not reduce peristomal infection after percutaneous endoscopic gastrostomy. CRP elevation of less than 3 mg/dl may be used to exclude the potential peristomal wound infection. Trial registration NCT04249570 ( https://clinicaltrials.gov/ct2/show/NCT04249570Test ).

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-230XTest

الوصول الحر: https://doaj.org/article/1780096686c7437983f1199ac04dbbbaTest

المؤلفون: Feng-Chi Kuo, Jerry Cheng-Yen Lai, Hui-Ru Shieh, Wan-Zu Liou, Ming-Jong Bair, Yu-Jen Chen

المصدر: Biomedicines, Vol 12, Iss 3, p 697 (2024)

مصطلحات موضوعية: cimetidine, tumor microenvironment, immunomodulation, Biology (General), QH301-705.5

الوصف: Histamine modulates immunity by binding to histamine receptor 2 (H2R). Cimetidine, an H2R antagonist that inhibits gastric acid secretion and treats gastrointestinal ulcers, interferes with histamine-mediated immunomodulation and may have anticancer activity. This study examined cimetidine’s effect on the anticancer effect of anti-PD-L1 in colon cancer. The MTT assay, colony formation assay, and DNA histograms assessed cell viability, clonogenicity, and cell cycle distribution, respectively. Flow cytometry measured H2R and PD-L1 expression and estimated specific immune cell lineages. For the in vivo study, tumor cells were subcutaneously implanted into the right flank of BALB/c mice. Cimetidine had no significant effect on CT26 cell viability, clonogenicity, or cell cycle distribution. It also did not affect H2R and PD-L1 expression levels in CT26 cells. In vivo, anti-PD-1 and anti-PD-L1 suppressed CT26 tumor growth, whereas cimetidine showed mild antitumor activity. In the combined experiment, cimetidine significantly attenuated anti-PD-1 and anti-PD-L1′ antitumor effects without major toxicity. In the tumor microenvironment, anti-PD-L1 increased CD3+ T, CD4+ T, and CD8+ T cells and M1 macrophages. Combined treatment with cimetidine reversed this. Cimetidine also reversed anti-PD-1 and anti-PD-L1′s decrease in circulating and tumor-associated neutrophils. Cimetidine attenuated anti-PD-L1′s antitumor effect and modulated the tumor microenvironment in colon cancer.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2227-9059/12/3/697Test; https://doaj.org/toc/2227-9059Test

الوصول الحر: https://doaj.org/article/0f24b00af425465eaea5dbfaa0e5ba4aTest

المؤلفون: Yu-Jen Chen, Jung-Tang Huang

المصدر: Biosensors and Bioelectronics: X, Vol 14, Iss , Pp 100374- (2023)

مصطلحات موضوعية: Functional colloidal gold, Virus detection, Rapid screening reagents, Functional hydrogel, Biotechnology, TP248.13-248.65

الوصف: Coronavirus disease 2019 (COVID-19) has significantly affected human life, and preventing the spread of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has become an important issue. Nowadays, rapid screening tests are primarily used to identify potentially infected patients and prevent viral transmission. At present, commercially available antibody-based rapid screening tests require the collection of respiratory tract samples. Sample collection is performed using methods that create discomfort. Therefore, we developed a lysis-free rapid screening test platform that can be fitted onto a medical mask. The mask can be worn for one day, after which the virus accumulates in the sampling pad. Finally, the wearer can directly activate the rapid screening test for self-monitoring by using their saliva. In this study, the structure of the novel lysis-free rapid screening test was similar to that of a commercial one. However, a lysis solution is added to the sample pad in advance and a hydrogel is added as a buffering material for viral detection. Our design improved the efficiency of combining the sample and aptamer as well as the detection efficiency. The H1N1 hemagglutinin and SARS-CoV-2 nucleocapsid protein were detected using the test platform which had a detection limit of 0.1 ng/ml. In addition, our design was validated via actual usage by patients with COVID-19 who had been ill for more than 7 d as well as healthcare workers who were close contacts and at high-risk of infection. The novel test platform can collect and accumulate viruses from patients with low viral load to achieve early viral detection and prevention of spread.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2590137023000717Test; https://doaj.org/toc/2590-1370Test

الوصول الحر: https://doaj.org/article/26383342b9a44e9783a78f2b4fb6b079Test

المؤلفون: Chih-Cheng Cheng, Yu-Jen Chen, Shin-Hung Lin, Hsin-Min Wang, Guang-Ping Lin, Tien-Kan Chung

المصدر: Micromachines, Vol 15, Iss 1, p 36 (2023)

مصطلحات موضوعية: magnetoelectric, multiferroic, nano Ni chevrons, piezoelectric PMN–PT, electric-field control, magnetic single domain, Mechanical engineering and machinery, TJ1-1570

الوصف: We report the magnetic-field-assisted electric-field-controlled domain switching of a magnetic single domain in a multiferroic/magnetoelectric Ni nanochevrons/[Pb(Mg1/3Nb2/3)O3]0.68–[PbTiO3]0.32 (PMN–PT) layered structure. Initially, a magnetic field was applied in the transverse direction across single-domain Ni nanochevrons to transform each of them into a two-domain state. Subsequently, an electric field was applied to the layered structure, exerting the converse magnetoelectric effect to transform/release the two-domain Ni nanochevrons into one of two possible single-domain states. Finally, the experimental results showed that approximately 50% of the single-domain Ni nanochevrons were switched permanently after applying our approach (i.e., the magnetization direction was permanently rotated by 180 degrees). These results mark important advancements for future nanoelectromagnetic systems.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2072-666X/15/1/36Test; https://doaj.org/toc/2072-666XTest

الوصول الحر: https://doaj.org/article/0ea6dda32ce14d10a5b2b27f4bcc4a1fTest

المؤلفون: Yu-Jen Chen, Jung-Tang Huang

المصدر: Biosensors and Bioelectronics: X, Vol 13, Iss , Pp 100316- (2023)

مصطلحات موضوعية: Aptamer, Methylene blue, Influenza capture, Virus detection, Bioaerosol, Lateral flow immunoassay, Biotechnology, TP248.13-248.65

الوصف: Many bacteria and viruses are spreading in the air in the living environment, and high concentrations of viruses entering the human body will cause harm. This research is committed to developing a virus collector, which is used to collect influenza and coronavirus in the air. In our system, the intake fan can beget negative pressure into the water circulation channel and bring the virus into it, and then the sensor chip will obtain the electrical signal. In this study, we successfully used methylene blue to simulate viruses in the air. The result of this experiment showed that the distance between the air virus collector and the atomizer was 30 cm in height, 60 cm & 90 cm in length. The capture efficiency was respectively 1.1% and 0.8%. Also, we use lateral flow immunochromatographic assay to detect the collected samples of Influenza H1N1 Hemagglutinin Protein, and the actual limit of detection is 16.97 ng/ml. In addition, the experiments also proved that the water circulation device in this study could accumulate the methylene blue samples onto the sensor. In the future, it can be used with the sensor chip as a virus detection platform which can collect and monitor viruses simultaneously. By using this device, people can be warned and take the necessary precautions to reduce the chance of the transmission of viruses.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2590137023000134Test; https://doaj.org/toc/2590-1370Test

الوصول الحر: https://doaj.org/article/4ecde04e51c049ce8f93a62abfc5b9ceTest