المؤلفون: Yuko S Niino, Ikuo Kawashima, Yoshinobu Iguchi, Hiroaki Kanda, Kiyoshi Ogura, Kaoru Mita-Yoshida, Tomio Ono, Maya Yamazaki, Kenji Sakimura, Satomi Yogosawa, Kiyotsugu Yoshida, Seiji Shioda, Takaya Gotoh

المصدر: PLoS ONE, Vol 16, Iss 7, p e0253912 (2021)

مصطلحات موضوعية: Medicine, Science

الوصف: Protein kinase C-delta (PKCδ) has a caspase-3 recognition sequence in its structure, suggesting its involvement in apoptosis. In addition, PKCδ was recently reported to function as an anti-cancer factor. The generation of a PKCδ knockout mouse model indicated that PKCδ plays a role in B cell homeostasis. However, the Pkcrd gene, which is regulated through complex transcription, produces multiple proteins via alternative splicing. Since gene mutations can result in the loss of function of molecular species required for each tissue, in the present study, conditional PKCδ knockout mice lacking PKCδI, II, IV, V, VI, and VII were generated to enable tissue-specific deletion of PKCδ using a suitable Cre mouse. We generated PKCδ-null mice that lacked whole-body expression of PKCδ. PKCδ+/- parental mice gave birth to only 3.4% PKCδ-/- offsprings that deviated significantly from the expected Mendelian ratio (χ2(2) = 101.7, P < 0.001). Examination of mice on embryonic day 11.5 (E11.5) showed the proportion of PKCδ-/- mice implanted in the uterus in accordance with Mendelian rules; however, approximately 70% of the fetuses did not survive at E11.5. PKCδ-/- mice that survived until adulthood showed enlarged spleens, with some having cardiac and pulmonary abnormalities. Our findings suggest that the lack of PKCδ may have harmful effects on fetal development, and heart and lung functions after birth. Furthermore, our study provides a reference for future studies on PKCδ deficient mice that would elucidate the effects of the multiple protein variants in mice and decipher the roles of PKCδ in various diseases.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/9aeae0a7358643b3bccee12c21878958Test

المؤلفون: Hideaki Shiraishi, Isamu Ozaki, Isao Hashimoto, Kyousuke Kamada, Masatoshi Takeda, Masayuki Hirata, Naohiro Tsuyuguchi, Nobukazu Nakasato, Ryouhei Ishii, Shigeki Kameyama, Yasunori Aoki, Yoshinobu Iguchi, Yutaka Watanabe, 中里 信和, 亀山 茂樹, 井口 義信, 尾﨑 勇, 平田 雅之, 橋本 勲, 武田 雅俊, 渡辺 裕貴, 白石 秀明, 石井 良平, 鎌田 恭輔, 露口 尚弘, 青木 保典

المصدر: 臨床神経生理学 / Japanese Journal of Clinical Neurophysiology. 2013, 41(1):29

المؤلفون: Hideaki Shiraishi, Isamu Ozaki, Isao Hashimoto, Kyosuke Kamata, Masayuki Hirata, Naohiro Tsuyuguchi, Ryohei Ishii, Shigeki Kameyama, Yoshinobu Iguchi, Yutaka Watanabe, 亀山 茂樹, 井口 義信, 尾﨑 勇, 平田 雅之, 橋本 勲, 渡辺 裕貴, 白石 秀明, 石井 良平, 鎌田 恭輔, 露口 尚弘

المصدر: 臨床神経生理学 / Japanese Journal of Clinical Neurophysiology. 2013, 41(2):57

المؤلفون: Hideaki Shiraishi, Hidetoshi Ikeda, Isamu Ozaki, Isao Hashimoto, Kyousuke Kamada, Masayuki Hirata, Naohiro Tsuyuguchi, Nobukazu Nakasato, Ryouhei Ishii, Shigeki Kameyama, Yoshinobu Iguchi, Yutaka Watanabe, 中里 信和, 亀山 茂樹, 井口 義信, 尾﨑 勇, 平田 雅之, 橋本 勲, 池田 英敏, 渡辺 裕貴, 白石 秀明, 石井 良平, 鎌田 恭輔, 露口 尚弘

المصدر: 臨床神経生理学 / Japanese Journal of Clinical Neurophysiology. 2013, 41(1):46

المؤلفون: Hideaki Shiraishi, Isamu Ozaki, Isao Hashimoto, Kyousuke Kamada, Masayuki Hirata, Naohiro Tsuyuguchi, Nobukazu Nakasato, Ryouhei Ishii, Shigeki Kameyama, Yoshinobu Iguchi, Yutaka Watanabe, 中里 信和, 亀山 茂樹, 井口 義信, 尾﨑 勇, 平田 雅之, 橋本 勲, 渡辺 裕貴, 白石 秀明, 石井 良平, 鎌田 恭輔, 露口 尚弘

المصدر: 臨床神経生理学 / Japanese Journal of Clinical Neurophysiology. 2012, 40(4):203

المؤلفون: Hideaki Shiraishi, Hidetoshi Ikeda, Isamu Ozaki, Isao Hashimoto, Kyousuke Kamada, Masayuki Hirata, Naohiro Tsuyuguchi, Nobukazu Nakasato, Ryouhei Ishii, Shigeki Kameyama, Shinichi Sakamoto, Takehiro Uda, Yoshinobu Iguchi, Yutaka Watanabe, 中里 信和, 亀山 茂樹, 井口 義信, 坂本 真一, 宇田 武弘, 尾﨑 勇, 平田 雅之, 橋本 勲, 池田 英敏, 渡辺 裕貴, 白石 秀明, 石井 良平, 鎌田 恭輔, 露口 尚弘

المصدر: 臨床神経生理学 / Japanese Journal of Clinical Neurophysiology. 2012, 40(4):195

المؤلفون: Chie Sato, Jinghua Huang, Masahito Nemoto, Yoko Hoshi, Yoshinobu Iguchi

المصدر: Proceedings of Annual Meeting of the Physiological Society of Japan. 2007, :245

المؤلفون: Haruhiko Kishima, Hideaki Shiraishi, Isamu Ozaki, Isao Hashimoto, Kyousuke Kamada, Masayuki Hirata, Naohiro Tsuyuguchi, Ryohei Ishii, Satoru Oshino, Shigeki Kameyama, Takufumi Yanagisawa, Tetsu Goto, Toshiki Yoshimine, Yoshinobu Iguchi, Yutaka Watanabe, 亀山 茂樹, 井口 義信, 吉峰 俊樹, 尾﨑 勇, 平田 雅之, 後藤 哲, 押野 悟, 柳澤 琢史, 橋本 勲, 渡辺 裕貴, 白石 秀明, 石井 良平, 貴島 晴彦, 鎌田 恭輔, 露口 尚弘

المصدر: 臨床神経生理学 / Japanese Journal of Clinical Neurophysiology. 2012, 40(3):140

المؤلفون: Hideaki Shiraishi, Isamu Ozaki, Isao Hashimoto, Kyousuke Kamada, Masato Yumoto, Masayuki Hirata, Naohiro Tsuyuguchi, Nobukazu Nakasato, Ryouhei Ishii, Shigeki Kameyama, Takashi Nagamine, Yoshinobu Iguchi, Yutaka Watanabe, 中里 信和, 亀山 茂樹, 井口 義信, 尾﨑 勇, 平田 雅之, 橋本 勲, 渡辺 裕貴, 湯本 真人, 白石 秀明, 石井 良平, 鎌田 恭輔, 長峯 隆, 露口 尚弘

المصدر: 臨床神経生理学 / Japanese Journal of Clinical Neurophysiology. 2012, 40(3):119

المؤلفون: Isamu Ozaki, Isao Hashimoto, Yoshinobu Iguchi, 井口 義信, 尾崎 勇, 橋本 勲

المصدر: 認知神経科学 / Japanese Journal of Cognitive Neuroscience. 2011, 13(1):1