المؤلفون: Yongping Huang, Ying Nie, Fei Zhou, Biansheng Li, Qiulan Luo, Bin Zhang, Qinpei Zeng, Yisheng Huang

المصدر: Food Science & Nutrition, Vol 12, Iss 3, Pp 2145-2152 (2024)

مصطلحات موضوعية: biological preservatives, chitosan, collagen, Nemipterus virgatus, ε‐Polylysine, Nutrition. Foods and food supply, TX341-641

الوصف: Abstract In order to evaluate the effects of chitosan, ε‐polylysine, and collagen on the preservation properties of refrigerated Nemipterus virgatus, samples were tested with different treatments for 10 days, namely chitosan, ε‐polylysine and collagen (CH + ε‐PL + CA), chitosan and ε‐polylysine (CH + ε‐PL), chitosan and collagen (CH + CA), ε‐polylysine and collagen (ε‐PL + CA), and the uncoated sample (CK). The results demonstrated that the bio‐coating exhibited better preservation effects. The CH + ε‐PL + CA, CH + ε‐PL, CH + CA, ε‐PL + CA treatments could significantly inhibit bacterial growth and retard the increase of total volatile base nitrogen (TVB‐N), 2‐thiobarbituric acid (TBA), K‐value, and total viable counts (TVC) in N. virgatus fillets. The pH of all samples decreased and reached its lowest value on day 6, then increased significantly at the end of the experiment (p

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2048-7177Test

الوصول الحر: https://doaj.org/article/fd73863b48274cceab7afab4133d7da6Test

المؤلفون: Yin Zhang, Qingfeng Miao, Ruiping Li, Minghai Sun, Xinmin Yang, Wei Wang, Yongping Huang, Weiying Feng

المصدر: Land, Vol 13, Iss 6, p 773 (2024)

مصطلحات موضوعية: Hetao Irrigation District, autumn irrigation, dry drainage, water and salt movement, Agriculture

الوصف: Autumn irrigation is a key measure for alleviating soil salinity and promoting sustainable agricultural development in the Hetao Irrigation district; however, only a part of farmland is irrigated in autumn during the non-growth period of crops, which leads to the redistribution of soil water and salt between autumn-irrigated land (AIL) and adjacent non-autumn-irrigated land (NAIL) after autumn irrigation. To explore the distribution and variation of soil water and salt in different positions of AIL and NAIL after local autumn irrigation and reveal the interaction range between AIL and NAIL, field experiments were carried out for two years in typical test areas. The results showed that compared with non-autumn irrigation, autumn irrigation improved the distribution uniformity of soil water and salt profiles in both horizontal and vertical directions; after autumn irrigation, the water content of the soil at the nearest sampling point to the boundary in the AIL increased the least, but the desalination rate was the greatest, while the water and salt contents of the soil within 45 m from the sampling points to the boundary in the NAIL both increased significantly. NAIL received the drainage of AIL and made the groundwater level after the rise in AIL fell quickly back, but unreasonable autumn irrigation caused the groundwater level of AIL to remain at a high level before freezing, exacerbating the risk of groundwater carrying salts to the surface soil during the freezing and thawing period, detrimental to the growth of crops in the next spring. The research results are of great significance to the rational use of farmland water resources and the improvement of soil salinization in cold and dry areas.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2073-445X/13/6/773Test; https://doaj.org/toc/2073-445XTest

الوصول الحر: https://doaj.org/article/f24ab36193a342fb82c2f37cf80f776dTest

المؤلفون: Lansa Qian, Xu Yang, Xiaomiao Xu, Dehong Yang, Chenxu Zhu, Meiyan Yi, Honglun Bi, Yaohui Wang, Yongping Huang

المصدر: PLoS Genetics, Vol 19, Iss 12, p e1011073 (2023)

مصطلحات موضوعية: Genetics, QH426-470

الوصف: The reproductive process in various species has undergone evolutionary adaptations at both the physiological and molecular levels, playing a significant role in maintaining their populations. In lepidopteran insects, the spermatophore is a unique structure formed in the female reproductive system, in which sperm storage and activation take place. It is known that the formation of the spermatophore is regulated by seminal fluid proteins derived from males. However, studies investigating the genetic mechanisms behind spermatophore formation in lepidopterans have been limited. In this study, our focus was on SPSL1, a gene that encodes a trypsin-type seminal fluid protein in Spodoptera frugiperda, a pest species with global invasive tendencies. Our findings revealed that SPSL1 expression was predominantly observed in the male reproductive tracts, and the disruption of this gene resulted in male sterility. Surprisingly, fluorescence analysis indicated that the absence of SPSL1 did not affect spermatogenesis or sperm migration within the male reproductive system. However, when females mated with SPSL1-mutant males, several defects were observed. These included disruptions in spermatophore formation, sperm activation in the copulatory bursae, and sperm migration into the spermathecae. Additionally, mass spectrometry analysis highlighted reduced levels of energy-related metabolites, suggesting that SPSL1 plays an essential role in promoting hydrolysis reactions during copulation. Consequently, our study demonstrates that SPSL1 is crucial for male fertility due to its functions in spermatophore formation and sperm activation. This research provides valuable insights into the genetic factors underlying reproductive processes in lepidopteran insects and sheds light on potential strategies for controlling invasive pest populations.

وصف الملف: electronic resource

العلاقة: https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1011073&type=printableTest; https://doaj.org/toc/1553-7390Test; https://doaj.org/toc/1553-7404Test

الوصول الحر: https://doaj.org/article/b7342e029785487a9979ded6dd8c46f5Test

المؤلفون: Xingyu Luo, Gangqi Fang, Kuangqin Chen, Yu Song, Tianyi Lu, Jeffery K. Tomberlin, Shuai Zhan, Yongping Huang

المصدر: mBio, Vol 14, Iss 5 (2023)

مصطلحات موضوعية: Hermetia illucens, Citrobacter amalonaticus, gut symbiont, host-microbe interaction, symbiont-mediated RNAi, intestinal protein metabolism, Microbiology, QR1-502

الوصف: ABSTRACT Black soldier fly (BSF), Hermetia illucens (L.) (Diptera: Stratiomyidae), is a promising bio-agent to transform organic wastes into valuable insect biomass, and the intestinal microbes play an essential role in this process. Here, we report that Citrobacter amalonaticus, a Gram-negative gut commensal bacterium of BSF larvae, can colonize the whole intestines of the larva and significantly promote the larval growth and development. The bacterial association with the larva had a marked impact on the larval transcriptome and modulated the expression of genes involved in dozens of biological pathways including host macro-nutrient metabolism and immune response. We investigated the underlying mechanisms driving the interaction and found that C. amalonaticus association can directly promote the expression of two gene families related to intestinal protein metabolism: Hitryp serine protease trypsin family and Himtp metallopeptidase family. To determine the gene function, we developed a symbiont-mediated double-strand RNA interference approach and successfully achieved gene knockdown in larval midgut in situ. Knockdown of the two gene families and protease inhibition in larval intestines attenuated the promoting effects on larval growth significantly. The results indicate that the gut symbiont promotes BSF larval growth partly via modulating the expression of the genes involved in intestinal protein metabolism. Taken together, this study establishes a novel genetic tool to study the insect functional genomics and the host-symbiont interaction and sheds light on the important roles of intestinal protein metabolism on the interaction. Importance Black solider fly larvae and the gut microbiota can recycle nutrients from various organic wastes into valuable insect biomass. We found that Citrobacter amalonaticus, a gut commensal bacterium of the insect, exerts beneficial effects on larval growth and development and that the expression of many metabolic larval genes was significantly impacted by the symbiont. To identify the larval genes involved in the host-symbiont interaction, we engineered the symbiont to produce double-strand RNA and enabled the strain to silence host genes in the larval gut environment where the interaction takes place. With this approach, we confirmed that two intestinal protease families are involved in the interaction and provided further evidence that intestinal protein metabolism plays a role in the interaction. This work expands the genetic toolkits available to study the insect functional genomics and host-symbiont interaction and provide the prospective for the future application of gut microbiota on the large-scale bioconversion.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2150-7511Test

الوصول الحر: https://doaj.org/article/c79ea088526540ce81d14d3fa0ff32d9Test

المؤلفون: Xiaoli Lin, Yongping Huang, Yuchun Rao, Linjuan Ouyang, Dahu Zhou, Changlan Zhu, Junru Fu, Chunlian Chen, Jianhua Yin, Jianmin Bian, Haohua He, Guoxing Zou, Jie Xu

المصدر: BMC Plant Biology, Vol 22, Iss 1, Pp 1-13 (2022)

مصطلحات موضوعية: Phytochrome C, Flowering time, Chlorophyll synthesis, Dimerization, Rice, Botany, QK1-989

الوصف: Abstract Background Phytochromes are important photoreceptors in plants, and play essential roles in photomorphogenesis. The functions of PhyA and PhyB in plants have been fully analyzed, while those of PhyC in plant are not well understood. Results A rice mutant, late heading date 3 (lhd3), was characterized, and the gene LHD3 was identified with a map-based cloning strategy. LHD3 encodes phytochrome C in rice. Animo acid substitution in OsphyC disrupted its interaction with OsphyB or itself, restraining functional forms of homodimer or heterodimer formation. Compared with wild-type plants, the lhd3 mutant exhibited delayed flowering under both LD (long-day) and SD (short-day) conditions, and delayed flowering time was positively associated with the day length via the Ehd1 pathway. In addition, lhd3 showed a pale-green-leaf phenotype and a slower chlorophyll synthesis rate during the greening process. The transcription patterns of many key genes involved in photoperiod-mediated flowering and chlorophyll synthesis were altered in lhd3. Conclusion The dimerization of OsPhyC is important for its functions in the regulation of chlorophyll synthesis and heading. Our findings will facilitate efforts to further elucidate the function and mechanism of OsphyC and during light signal transduction in rice.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2229Test

الوصول الحر: https://doaj.org/article/b5eff14138d34979ad5a77971fd2c646Test

المؤلفون: Xiaoyan Cheng, Yongping Huang, Yong Tan, Lin Tan, Jianhua Yin, Guoxing Zou

المصدر: Rice, Vol 15, Iss 1, Pp 1-12 (2022)

مصطلحات موضوعية: Rice breeding, Dwarfing genes, Semi-dwarfing genes, Plant culture, SB1-1110

الوصف: Abstract The “Green revolution” gene sd1 has been used widely in the breeding of modern rice varieties for over half a century. The application of this gene has increased rice yields and thereby supported a significant proportion of the global population. The use of a single gene, however, has raised concerns in the scientific community regarding its durability, especially given the bottleneck in genetic background and the need for large input of fertilizer. New dwarfing or semi-dwarfing genes are needed to alleviate our dependence on the sole “Green revolution” gene. In the past few years, several new dwarfing and semi-dwarfing genes as well as their mutants have been reported. Here, we provide an extensive review of the recent discoveries concerning newly identified genes that are potentially useful in rice breeding, including methods employed to create and effectively screen new rice mutants, the phenotypic characteristics of the new dwarfing and semi-dwarfing mutants, potential values of the new dwarfing and semi-dwarfing genes in rice breeding, and potential molecular mechanisms associated with the newly identified genes.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1939-8425Test; https://doaj.org/toc/1939-8433Test

الوصول الحر: https://doaj.org/article/ed2d97d15a984ae7925b50b6a18cd671Test

المؤلفون: Yongping Huang, Xinlong Yao, Caili Dai, Yining Wu, Lin Li, Bin Yuan

المصدر: Gels, Vol 10, Iss 1, p 2 (2023)

مصطلحات موضوعية: supramolecular reinforced gel, gel fracturing fluid, deep reservoir, gel-breaking solution, low permeability damage, Science, Chemistry, QD1-999, Inorganic chemistry, QD146-197, General. Including alchemy, QD1-65

الوصف: Gel fracturing fluid is the optimum fracturing fluid for proppant suspension, which is commonly applied in deep reservoir hydraulic fracturing. The content of polymers and crosslinkers in gel fracturing fluid is usually high to meet the needs of high-temperature resistance, leading to high costs and reservoir permeability damage caused by incomplete gel-breaking. In this paper, a supramolecular reinforced gel (SRG) fracturing fluid was constructed by strengthening the supramolecular force between polymers. Compared with single network gel (SNG) fracturing fluid, SRG fracturing fluid could possess high elasticity modulus (G′ = 12.20 Pa) at lower polymer (0.4 wt%) and crosslinker (0.1 wt%) concentrations. The final viscosity of SRG fracturing fluid was 72.35 mPa·s, meeting the temperature resistance requirement of gel fracturing fluid at 200 °C. The gel-breaking time could be extended to 90–120 min using an encapsulated gel breaker. Gel particles are formed after the gel fracturing fluid is broken. The median particle size of gel particles in the SRG-breaking solution was 126 nm, which was much smaller than that in the industrial gel (IDG) breaking fluid (587 nm). The damage of the SRG-breaking solution to the core permeability was much less than the IDG-breaking solution. The permeability damage of cores caused by the SRG-breaking solutions was only about half that of IDG-breaking solutions at 1 mD.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2310-2861/10/1/2Test; https://doaj.org/toc/2310-2861Test

الوصول الحر: https://doaj.org/article/3e9ddc514d7a49ee888878f58e0acd24Test

المؤلفون: Yaqun Liu, Jiaoyun Jiang, Zhenxia Zhang, Qionglu Huang, Lianghui Chen, Yongping Huang, Xianghui Zou, Ying Nie, Mouquan Liu, Yuzhong Zheng

المصدر: CyTA - Journal of Food, Vol 22, Iss 1 (2024)

مصطلحات موضوعية: Phyllanthus emblica L., association rule mining, composition, target, efficacy, anticancer, Nutrition. Foods and food supply, TX341-641, Food processing and manufacture, TP368-456

الوصف: ABSTRACTPhyllanthus emblica L. (P. emblica L.) is a widely consumed healthy fruit known for its taste and benefits. This study used association rule mining to demonstrate its anticancer properties, analyzing its 65 chemical components, 1767 related targets, and 4282 efficacy items. Myricetin was identified as a key component targeting CA2, which is involved in cancer pathways. The research suggested P. emblica L.’s potential against non-small cell lung cancer and esophageal cancer, which was supported by experiments showing significant growth inhibition in related cancer cells. This approach offers a more targeted and convincing understanding of P. emblica L.’s effects, improving upon traditional food research methods.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1947-6337Test; https://doaj.org/toc/1947-6345Test

الوصول الحر: https://doaj.org/article/42abbe8dacae446ab548e62716e49871Test

المؤلفون: Guohai Wang, Yang Huang, Wei Yao, Qiuchan Huang, Yongping Huang, Lijuan Wei, Qihai Zhou

المصدر: PeerJ, Vol 11, p e15028 (2023)

مصطلحات موضوعية: Interaction network, Urban ecosystem, Functional diversity, Fleshy fruit plant, Frugivorous birds, Medicine, Biology (General), QH301-705.5

الوصف: The interaction between plants and frugivores is crucial to ecosystem function and community diversity. However, little is known about the interaction between plants and frugivorous bird species in urban green spaces. We observed interactions between plants and frugivorous birds in the Guilin Botanical Garden for one year and determined the structure and characteristics of the interaction network. We also analyzed the impact of species traits on their network roles. Interactions between 14 frugivorous birds and 13 fruit plant species were recorded in the study area. Autumn interactions comprised 38.79% of the overall network, and winter interactions comprised 33.15%. The modularity (Q, z-score) of the network was higher in autumn; the weighted nestedness (wNODF, z-score) and interaction evenness (E2, z-score) of the network were higher in winter; the connectance (C, z-score) and interaction diversity (z-score) of the network were higher in spring; and the specialization (H2′, z-score) of the network was higher in summer. The observed network showed lower C, lower interaction H2, lower E2 , lower wNODF, higher H2′ and higher Q when compared to the random networks. The bird species most important to network stability were Hemixos castanonotus, Parus venustulus, and Pycnonotus sinensis. The most important plant species were Alocasia macrorrhiza, Cinnamomum camphora, and Machilus nanmu. Of all the bird and plant traits included in this study, only plant color had a significant impact on species strength, with black fruit having a higher species strength. Our results suggest that interaction networks in urban green spaces can be temporally complex and variable and that a network approach can be an important monitoring tool for detecting the status of crucial ecosystem functions.

وصف الملف: electronic resource

العلاقة: https://peerj.com/articles/15028.pdfTest; https://peerj.com/articles/15028Test/; https://doaj.org/toc/2167-8359Test

الوصول الحر: https://doaj.org/article/ea0bdd5f8a284cd5b7f83e2c79584439Test

المؤلفون: Qiulan Luo, Guangcai Zha, Liyun Lin, Yongping Huang, Xianghui Zou

المصدر: Food Science & Nutrition, Vol 10, Iss 3, Pp 936-944 (2022)

مصطلحات موضوعية: amino acids, climbing perch Anabas testudineus, crucian carp, freshwater fish, tissues, Nutrition. Foods and food supply, TX341-641

الوصف: Abstract This study aimed to investigate nutrition in climbing perch Anabas testudineus which is an important nutritious economic freshwater fish in Asia and compare with Carassius auratus (crucian carp). Three kinds of tissues, including muscle, livers, and eggs, were isolated, respectively. Physicochemical properties including moisture, ash, protein, amino acids, fat, vitamins, and calcium contents in those tissues were determined. The results showed climbing perch muscle and liver contained less moisture, but more protein, amino acids, and vitamins than crucian carp muscle and liver. While moisture, ash, protein, and total amino acids contents of climbing perch egg were lower than those of crucian carp egg. Climbing perch egg had more fat, vitamins, and calcium than crucian carp egg. The amino acid profile was also performed, and 16 amino acids were identified and quantified in muscle, liver, and egg. Among tissues, the highest and lowest concentration of total amino acid content was found in crucian carp eggs and livers, respectively. Glutamic acid (Glu) and histidine (His) were the most and least amino acids in climbing perch and crucian carp tissues, respectively. Sixteen amino acids in climbing perch egg were less than those in crucian carp egg while it is an opposite case in muscle and liver, which amino acids of climbing perch tissues were more than those of crucian carp muscle and liver. Vitamin A of climbing perch was more than crucian carp in all three tissues, but vitamin E content in climbing perch liver was lower than that of crucian carp liver. Calcium content of muscle had no difference between two species. The abovementioned comparison of physicochemical properties of different tissues from China climbing perch and crucian carp will provide a necessary supplementary of freshwater muscle nutrition research, also was helpful for application of climbing perch.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2048-7177Test

الوصول الحر: https://doaj.org/article/6ae9fa7bf98d4e35a43e343fb8d19321Test