المؤلفون: Yijing Liu, Lin Yu, Zhongzhi Yao, Yunwen Shen, Yiwen Pan

المصدر: Frontiers in Marine Science, Vol 11 (2024)

مصطلحات موضوعية: turbulence, phytoplankton, diatom, Thalassiosira pseudonana, Skeletonema costatum, Phaeodactylum tricornutum, Science, General. Including nature conservation, geographical distribution, QH1-199.5

الوصف: The effects of turbulence on phytoplankton growth have received considerable attentions. However, the complexity of turbulence poses a significant challenge to its systematic characterization in the laboratory, resulting in relatively limited data on the effects of turbulence on several algal species. Here, a laboratory turbulence simulation system was set up to systematically investigate the growth of three common diatom species (Thalassiosira pseudonana, Skeletonema costatum, and Phaeodactylum tricornutum) under stationary and turbulent conditions (at 60, 120, 180 rpm), and measurements were taken for the algal biomass, algal photosynthetic activity, and nutrients consumption. By comparing the growth of three algae species under different turbulence exposure intensities, this study found that different algae exhibit varying sensitivities to turbulence, and therefore have different shear thresholds. Meanwhile, cell morphology is the key factor influencing the different shear threshold values observed in the three diatom species. Additionally, turbulence could impact algal aggregation and light availability, and dramatically improve nutrient uptake by phytoplankton. Our study will provides theoretical support for future endeavors in using turbulence to cultivate phytoplankton or combat algal blooms.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fmars.2024.1400798/fullTest; https://doaj.org/toc/2296-7745Test

الوصول الحر: https://doaj.org/article/aa2bc53930d64f639ae5c369b2eb224dTest

المؤلفون: Rongfu Tu, Ye Kang, Yiwen Pan, Yanyan Da, Doudou Ren, Ru Zhang, Zeqiong Cai, Yijia Liu, Jiao Xu, Junpeng Ma, Zhiyong Zhou, Shupeng Yin, Xiaozhuang Li, Peng Zhang, Qi Zhang, Jingchao Wang, Xinlan Lu, Chengsheng Zhang

المصدر: Cancer Cell International, Vol 24, Iss 1, Pp 1-14 (2024)

مصطلحات موضوعية: Gastric Cancer, USP29, AURKB, FUBP1, Targeted therapy, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282, Cytology, QH573-671

الوصف: Abstract Background Gastric cancer is a highly prevalent cancer type and the underlying molecular mechanisms are not fully understood. Ubiquitin-specific peptidase (USP) 29 has been suggested to regulate cell fate in several types of cancer, but its potential role in gastric carcinogenesis remains unclear. Methods The expression of USP29 in normal and gastric cancer tissues was analyzed by bioinformatics analysis, immunohistochemistry and immunoblot. Gene overexpression, CRISPR-Cas9 technology, RNAi, and Usp29 knockout mice were used to investigate the roles of USP29 in cell culture, xenograft, and benzo[a]pyrene (BaP)-induced gastric carcinogenesis models. We then delineated the underlying mechanisms using mass spectrometry, co-immunoprecipitation (Co-IP), immunoblot, ubiquitination assay, chromatin immunoprecipitation (ChIP), quantitative real-time PCR (qRT-PCR), and luciferase assays. Results In this study, we found that USP29 expression was significantly upregulated in gastric cancers and associated with poor patient survival. Ectopic expression of USP29 promoted, while depletion suppressed the tumor growth in vitro and in vivo mouse model. Mechanistically, transcription factor far upstream element binding protein 1 (FUBP1) directly activates USP29 gene transcription, which then interacts with and stabilizes aurora kinase B (AURKB) by suppressing K48-linked polyubiquitination, constituting a FUBP1-USP29-AURKB regulatory axis that medicates the oncogenic role of USP29. Importantly, systemic knockout of Usp29 in mice not only significantly decreased the BaP-induced carcinogenesis but also suppressed the Aurkb level in forestomach tissues. Conclusions These findings uncovered a novel FUBP1-USP29-AURKB regulatory axis that may play important roles in gastric carcinogenesis and tumor progression, and suggested that USP29 may become a promising drug target for cancer therapy.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1475-2867Test

الوصول الحر: https://doaj.org/article/9ca5f6d9e7074710b796d50f37a670c0Test

المؤلفون: Zong‐Pei Jiang, Cao Qin, Yiwen Pan, Chengfeng Le, Nancy Rabalais, Robert Eugene Turner, Katja Fennel, Kui Wang, Wei‐Jun Cai

المصدر: Geophysical Research Letters, Vol 51, Iss 5, Pp n/a-n/a (2024)

مصطلحات موضوعية: acidification, Gulf of Mexico, hypoxia, decadal, eutrophication, climate change, Geophysics. Cosmic physics, QC801-809

الوصف: Abstract Coastal waters often experience enhanced ocean acidification due to the combined effects of climate change and regional biological and anthropogenic activities. Through reconstructing summertime bottom pH in the northern Gulf of Mexico from 1986 to 2019, we demonstrated that eutrophication‐fueled respiration dominated bottom pH changes on intra‐seasonal and interannual timescales, resulting in recurring acidification coinciding with hypoxia. However, the multi‐decadal acidification trend was principally driven by rising atmospheric CO2 and ocean warming, with more acidified and less buffered hypoxic waters exhibiting a higher rate of pH decline (−0.0023 yr−1) compared to non‐hypoxic waters (−0.0014 yr−1). The cumulative effect of climate‐driven decrease in pH baseline is projected to become more significant over time, while the potential eutrophication‐induced seasonal exacerbation of acidification may lessen with decreasing oxygen availability resulting from ocean warming. Mitigating coastal acidification requires both global reduction in CO2 emissions and regional management of riverine nutrient loads.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/0094-8276Test; https://doaj.org/toc/1944-8007Test

الوصول الحر: https://doaj.org/article/a95c726d347b4c1ca9078e7b077dc9a5Test

المؤلفون: Shuangshuang Wu, Jianping Chen, Chenli Xue, Yiwen Pan, Cheng Zhang

المصدر: Remote Sensing, Vol 16, Iss 10, p 1812 (2024)

مصطلحات موضوعية: lunar surface, LRO Diviner, Christiansen feature, lunar oxide, Chang’e-5, Science

الوصف: The chemical distribution on the lunar surface results from the combined effects of both endogenic and exogenic geological processes. Exploring global maps of chemical composition helps to gain insights into the compositional variation among three major geological units, unraveling the geological evolution of the Moon. The existing oxide abundance maps were obtained from spectral images of remote sensing and geochemical data from samples returned by Apollo and Luna, missing the chemical characteristics of the Moon’s late critical period. In this study, by adding geochemical data from Chang’e (CE)-5 lunar samples, we construct inversion models between the Christiansen feature (CF) and oxide abundance of lunar samples using the particle swarm optimization–extreme gradient boosting (PSO-XGBoost) algorithm. Then, new global oxide maps (Al2O3, CaO, FeO, and MgO) and Mg# with the resolution of 32 pixels/degree (ppd) were produced, which reduced the space weathering effect to some extent. The PSO-XGBoost models were compared with partial least square regression (PLSR) models and four previous results, indicating that PSO-XGBoost models possess the capability to effectively describe nonlinear relationships between CF and oxide abundance. Furthermore, the average contents of our results and the Diviner results for 21 major maria demonstrate high correlations, with R2 of 0.95, 0.82, 0.95, and 0.86, respectively. In addition, a new Mg# map was generated, which reveals different magmatic evolutionary processes in the three geologic units.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2072-4292/16/10/1812Test; https://doaj.org/toc/2072-4292Test

الوصول الحر: https://doaj.org/article/498e968dcb514d02baddb33f6edec05dTest

المؤلفون: Xiaotang Di, Yiwen Pan, Jinhua Yan, Jing Liu, Doudou Wen, Hao Jiang, Shubing Zhang

المصدر: Heliyon, Vol 10, Iss 1, Pp e23165- (2024)

مصطلحات موضوعية: Multiple myeloma, PIK3CG, c-Myc, Melphalan, Targeted therapy, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: Multiple myeloma (MM) is a malignant plasma cell disease. The activity of PIK3CG (PI3K catalytic subunit γ) is regulated directly by G-protein-coupled receptor and has been confirmed to be highly expressed in MM cells. This study aimed to determine the effect of pharmacological inhibition of PIK3CG on MM. We found that different concentrations of the PIK3CG inhibitor AS-605240 could suppress the growth of MM cell lines and the expression of c-Myc. The combination of PIK3CG inhibitor and the chemotherapy Melphalan could effectively inhibit the proliferation and migration of MM cells, promote the cell apoptosis, and decrease the ratio of Bcl-2/Bax and the expression of vimentin. The expression of proto-oncogene c-Myc was decreased and the sensitivity of cells to chemotherapeutic drugs was enhanced. Collectively, PIK3CG regulates growth of MM via c-Myc pathway, thus emerging as a promising molecular targeted therapy.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844023103732Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/1445513486fd4851b2c5aa72cc33a940Test

المؤلفون: Ye Kang, Yahuan Yu, Yijia Liu, Yiwen Pan, Ru Zhang, Doudou Ren, Zeqiong Cai, Junpeng Ma, Xiaofan Xiong, Qi Zhang, Chengsheng Zhang, Rongfu Tu

المصدر: Cancer Biology & Therapy, Vol 24, Iss 1 (2023)

مصطلحات موضوعية: usp29, cancer metabolism, nucleotide biosynthesis, neuroblastoma, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Cancer cells show enhanced nucleotide biosynthesis, which is essential for their unlimited proliferation, but the underlying mechanisms are not entirely clear. Ubiquitin specific peptidase 29 (USP29) was reported to sustain neuroblastoma progression by promoting glycolysis and glutamine catabolism; however, its potential role in regulating nucleotide biosynthesis in tumor cells remains unknown. In this study, we depleted endogenous USP29 in MYCN-amplified neuroblastoma SK-N-BE2 cells by sgRNAs and conducted metabolomic analysis in cells with or without USP29 depletion, we found that USP29 deficiency caused a disorder of intermediates involved in glycolysis and nucleotide biosynthesis. De novo nucleotide biosynthesis was analyzed using 13C6 glucose as a tracer under normoxia and hypoxia. The results indicated that USP29-depleted cells showed inhibition of nucleotide anabolic intermediates derived from glucose, and this inhibition was more significant under hypoxic conditions. Analysis of RNA sequencing data in SK-N-BE2 cells demonstrated that USP29 promoted the gene expression of metabolic enzymes involved in nucleotide anabolism, probably by regulating MYC and E2F downstream pathways. These findings indicated that USP29 is a key regulator of nucleotide biosynthesis in tumor cells.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1538-4047Test; https://doaj.org/toc/1555-8576Test

الوصول الحر: https://doaj.org/article/7d06242432c048e9b2de3e6d62442553Test

المؤلفون: Zhimin Xue, Danyu Wu, Jiefang Zhang, Yiwen Pan, Rongsheng Kan, Jing Gao, Binquan Zhou

المصدر: Heliyon, Vol 9, Iss 11, Pp e21309- (2023)

مصطلحات موضوعية: Coronary heart disease, Procyanidin B2, Cardiomyocyte hypoxia, Oxidative stress, Apoptosis, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: Background: Cardiomyocyte ischemia and hypoxia are important causes of oxidative stress damage and cardiomyocyte apoptosis in coronary heart disease (CHD). Epidemiological investigation has shown that eating more plant-based foods, such as vegetables and fruits, may significantly decrease the risk of CHD. As natural antioxidants, botanicals have fewer toxic side effects than chemical drugs and have great potential for development. Procyanidin B2 (PB2) is composed of flavan-3-ol and epicatechin and has been reported to have antioxidant and anti-inflammatory effects. However, whether PB2 exerts protective effects on hypoxic cardiomyocytes has remained unclear. This study aimed to explore the protective effect of PB2 against cardiomyocyte hypoxia and to provide new treatment strategies and ideas for myocardial ischemia and hypoxia in CHD. Methods and results: A hypoxic cardiomyocyte model was constructed, and a CCK-8 assay proved that PB2 had a protective effect on cardiomyocytes in a hypoxic environment. DCFH fluorescence staining, DHE staining, and BODIPY lipid oxidation assessment revealed that PB2 reduced the oxidative stress levels of cardiomyocytes under hypoxic conditions. TUNEL staining, Annexin V/PI fluorescence flow cytometry, and Western blot analysis of the expression of the apoptosis marker protein cleaved caspase-3 confirmed that PB2 reduced cardiomyocyte apoptosis under hypoxic conditions. JC-1 staining indicated that PB2 reduced the mitochondrial membrane potential of cardiomyocytes under hypoxia. In addition, transcriptomic analysis proved that the expression of 158 genes in cardiomyocytes was significantly changed after PB2 was added during hypoxia, of which 53 genes were upregulated and 105 genes were downregulated. GO enrichment analysis demonstrated that the activity of cytokines, extracellular matrix proteins and other molecules was changed significantly in the biological process category. KEGG enrichment analysis showed that the IL-17 signaling pathway and JAK-STAT signaling pathway underwent significant changes. We also performed metabolomic analysis and found that the levels of 51 metabolites were significantly changed after the addition of PB2 to cardiomyocytes during hypoxia. Among them, 39 metabolites exhibited increased levels, while 12 metabolites exhibited decreased levels. KEGG enrichment analysis showed that cysteine and methionine metabolism, arginine and proline metabolism and other metabolic pathways underwent remarkable changes. Conclusion: This study proves that PB2 can reduce the oxidative stress and apoptosis of cardiomyocytes during hypoxia to play a protective role. Transcriptomic and metabolomic analyses preliminarily revealed signaling pathways and metabolic pathways that are related to its protective mechanism. These findings lay a foundation for further research on the role of PB2 in the treatment of CHD and provide new ideas and new perspectives for research on PB2 in the treatment of other diseases.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844023085171Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/1f46dab2a57f4b2682feed28ef45ec91Test

المؤلفون: Cheng Zhang, Jianping Chen, Yiwen Pan, Shuangshuang Wu, Jian Chen, Xiaoxia Hu, Yue Pang, Xueting Liu, Ke Wang

المصدر: Remote Sensing, Vol 16, Iss 4, p 634 (2024)

مصطلحات موضوعية: mare basalts, Oceanus Procellarum, geomorphology, mineralogy, crater size-frequency distribution (CSFD), Science

الوصف: Mare basalts on the lunar surface are tangible expressions of the complex thermal evolution and geological processes that have occurred within the lunar interior. These basaltic manifestations are highly important because they provide invaluable insights into lunar geological evolution. Notably, the Oceanus Procellarum region, which is renowned for its extensive and long-lasting basaltic volcanism, is a premier location to investigate late-stage lunar thermal evolution. The primary aim of this research is to elucidate the geomorphological, compositional, and temporal attributes that define the mare basalts within the Oceanus Procellarum region. To achieve this aim, we comprehensively analyzed the geomorphological features present within the region, leveraging Kaguya/SELENE TC images and digital elevation models. Specifically, these geomorphological features encompass impact craters, wrinkle ridges, sinuous rilles, and volcanic domes. Subsequently, we thoroughly examined the mineralogical attributes of basalts in the Oceanus Procellarum region, leveraging Kaguya/SELENE MI data and compositional map products. To more accurately reflect the actual ages of the mare basalts in the Oceanus Procellarum region, we carefully delineated the geological units within the area and employed the latest crater size-frequency distribution (CSFD) technique to precisely determine their ages. This refined approach allowed for a more comprehensive and accurate understanding of the basaltic rocks in the study area. Overall, our comprehensive study included an in-depth analysis of the volcanic activity and evolution of the Oceanus Procellarum region, along with an examination of the correlation between the mineralogical composition and ages of mare basalts. The findings from this exhaustive investigation reveal a definitive age range for basalt units within the Oceanus Procellarum region from approximately 3.69 Ga to 1.17 Ga. Moreover, the latest mare basalts that formed were pinpointed north of the Aristarchus crater. Significantly, the region has experienced at least five distinct volcanic events, occurring approximately 3.40 Ga, 2.92 Ga, 2.39 Ga, 2.07 Ga, and 1.43 Ga, leading to the formation of multiple basalt units characterized by their unique mineral compositions and elemental abundances. Through the application of remote sensing mineralogical analysis, three primary basalt types were identified: low-titanium, very-low-titanium, and intermediate-titanium basalt. Notably, the younger basalt units exhibit an elevated titanium proportion, indicative of progressive olivine enrichment. Consequently, these younger basalt units exhibit more intricate and complex mineral compositions, offering valuable insights into the dynamic geological processes shaping the lunar surface.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2072-4292/16/4/634Test; https://doaj.org/toc/2072-4292Test

الوصول الحر: https://doaj.org/article/513b4b3e20b84afe85fc9fcbcff67a42Test

المؤلفون: Yiwen Pan, Guoguo Shang, Jing Li, Yuwen Zhang, Jianying Liu, Yuan Ji, Jing Ding, Xin Wang

المصدر: Frontiers in Immunology, Vol 14 (2023)

مصطلحات موضوعية: inborn errors of immunity, whole genomic sequencing, metagenomic sequencing, immune monitoring, IRF2BP2, Immunologic diseases. Allergy, RC581-607

الوصف: IntroductionInborn errors of immunity (IEI) are a heterogeneous group of disorders characterized by increased risk of infections, autoimmunity, autoinflammatory diseases, malignancy and allergy. Next-generation sequencing has revolutionized the identification of genetic background of these patients and assists in diagnosis and treatment. In this study, we identified a probable unique monogenic cause of IEI, and evaluated the immunological methods and pathogenic detections.MethodsA family with a member with a clinical diagnosis of IEI was screened by whole genomic sequencing (WGS). Demographic data, clinical manifestations, medical history, physical examination, laboratory findings and imaging features of the patient were extracted from medical records. Comprehensive immune monitoring methods include a complete blood count with differential, serum levels of cytokines and autoantibodies, T-cell and B-cell subsets analysis and measurement of serum immunoglobulins. In addition, metagenomic sequencing (mNGS) of blood, cerebrospinal fluid and biopsy from small intestine were used to detect potential pathogens.ResultsThe patient manifested with recurrent infections and autoimmune disorders, who was eventually diagnosed with IEI. Repetitive mNGS tests of blood, cerebrospinal fluid and biopsy from small intestine didn’t detect pathogenic microorganism. Immunological tests showed a slightly decreased level of IgG than normal, elevated levels of tumor necrosis factor and interleukin-6. Lymphocyte flow cytometry showed elevated total B cells and natural killer cells, decreased total T cells and B-cell plasmablasts. WGS of the patient identified a novel heterozygous mutation in IRF2BP2 (c.439_450dup p. Thr147_Pro150dup), which was also confirmed in his father. The mutation was classified as variant of uncertain significance (VUS) according to the American College of Medical Genetics and Genomics guidelines.ConclusionWe identified a novel IRF2BP2 mutation in a family with a member diagnosed with IEI. Immune monitoring and WGS as auxiliary tests are helpful in identifying genetic defects and assisting diagnosis in patients with clinically highly suspected immune abnormalities and deficiencies in inflammation regulation. In addition, mNGS techniques allow a more comprehensive assessment of the pathogenic characteristics of these patients. This report further validates the association of IRF2BP2 deficiency and IEI, and expands IEI phenotypes.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fimmu.2023.967345/fullTest; https://doaj.org/toc/1664-3224Test

الوصول الحر: https://doaj.org/article/f37116c9c8ad4c6e806a1b0c191713b8Test

المؤلفون: Yiwen Pan, Yu Feng, Weifeng Peng, Yang Cai, Jing Ding, Xin Wang

المصدر: BMC Neurology, Vol 22, Iss 1, Pp 1-10 (2022)

مصطلحات موضوعية: Status epilepticus, Seizure duration, Mortality, Seizure recurrence, Functional status, Neurology. Diseases of the nervous system, RC346-429

الوصف: Abstract Background In 2015, the International League Against Epilepsy proposed a new conceptual definition of status epilepticus (SE) with two operational dimensions (t1 and t2) to guide emergency treatment. The purpose of this study was to compare clinical characteristics and prognoses of patients at these two different time points. Methods We conducted a prospective observational cohort study of consecutive adults diagnosed with SE. In case of convulsive SE, t1 is 5 min and t2 is 30 min, whereas in case of focal SE with impaired consciousness, t1 is 10 min, t2 is 60 min. Data on clinical characteristics, including age, gender, history of prior seizures, neuroimaging, semiology, duration, and etiology of SE, were collected. The primary outcome was mortality, with seizure recurrence as a secondary measure, and functional status as tertiary outcome of enrolled patients at 3 months after SE onset. Results We screened one hundred patients with SE, with a median age of 66 years and 61% were male. Fifty-six (56.0%) patients reached t1 of SE, while 44 (44.0%) reached t2 of SE. Convulsive SE (52.0%, n = 52) was more common than focal SE with impaired consciousness (48.0%, n = 48). Status epilepticus secondary to an acute symptomatic process was the most common (50%, n = 50). Patients meeting t2 of SE demonstrated a remarkably increased risk of mortality (unadjusted analysis-RR 3.606, 95%CI 1.552–8.376, p = 0.003; adjusted analysis-RR 2.924, 95%CI 1.221–7.003, p = 0.016) and unfavorable functional status (unadjusted analysis-RR 1.803, 95%CI 1.280–2.539, p = 0.001; adjusted analysis-RR 1.664, 95%CI 1.184–2.340, p = 0.003) at 3 months compared to those who only reached t1 of SE. Patients reaching t2 of SE were more likely to experience seizure recurrence, however, there was no significant difference between the two cohorts. Conclusions Our study provides strong support for the new definition of SE. Patients meeting t2 of SE tend to have a remarkably increased risk of mortality and unfavorable functional outcomes compared to those who only reached t1 of SE. Furthermore, patients were likely to experience seizure recurrence after undergoing an episode of SE. Physicians must be educated about prompt recognition and appropriate management of SE.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2377Test

الوصول الحر: https://doaj.org/article/5968bba6e416405fb58aea3637b6f761Test