المؤلفون: Kun Hu, Qiao Dai, Babatope Samuel Ajayo, Hao Wang, Yufeng Hu, Yangping Li, Huanhuan Huang, Hanmei Liu, Yinghong Liu, Yayun Wang, Lei Gao, Ying Xie

المصدر: BMC Genomics, Vol 24, Iss 1, Pp 1-15 (2023)

مصطلحات موضوعية: Genome-wide identification, Wall associated kinase, Gibberellic acid, Transcriptional factor, Maize Kernel, Biotechnology, TP248.13-248.65, Genetics, QH426-470

الوصف: Abstract Background The functional roles of the Wall Associated Kinase (WAK) and Wall Associated Kinase Like (WAKL) families in cellular expansion and developmental processes have been well-established. However, the molecular regulation of these kinases in maize development is limited due to the absence of comprehensive genome-wide studies. Results Through an in-depth analysis, we identified 58 maize WAKL genes, and classified them into three distinct phylogenetic clusters. Moreover, structural prediction analysis showed functional conservation among WAKLs across maize. Promoter analysis uncovered the existence of cis-acting elements associated with the transcriptional regulation of ZmWAKL genes by Gibberellic acid (GA). To further elucidate the role of WAKL genes in maize kernels, we focused on three highly expressed genes, viz ZmWAKL38, ZmWAKL42 and ZmWAKL52. Co-expression analyses revealed that their expression patterns exhibited a remarkable correlation with GA-responsive transcription factors (TF) TF5, TF6, and TF8, which displayed preferential expression in kernels. RT-qPCR analysis validated the upregulation of ZmWAKL38, ZmWAKL42, ZmWAKL52, TF5, TF6, and TF8 following GA treatment. Additionally, ZmWAKL52 showed significant increase of transcription in the present of TF8, with ZmWAKL52 localizing in both the plasma membrane and cell wall. TF5 positively regulated ZmWAKL38, while TF6 positively regulated ZmWAKL42. Conclusions Collectively, these findings provide novel insights into the characterization and regulatory mechanisms of specific ZmWAKL genes involved in maize kernel development, offering prospects for their utilization in maize breeding programs.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2164Test

الوصول الحر: https://doaj.org/article/76c42169f7624ddfb91bb4c8032f36b2Test

المؤلفون: Sidi Xie, Ran Tian, Hanmei Liu, Yangping Li, Yufeng Hu, Yubi Huang, Junjie Zhang, Yinghong Liu

المصدر: Plants, Vol 13, Iss 12, p 1592 (2024)

مصطلحات موضوعية: maize, kernel length, expressing regulation, transcription factor, miRNA, Botany, QK1-989

الوصف: The kernel length is a crucial determinant of maize (Zea mays L.) yield; however, only a limited number of genes regulating kernel length have been validated, thus leaving our understanding of the mechanisms governing kernel length incomplete. We previously identified a maize kernel mutant, defective kernel219 (dek219), which encodes the DICER-LIKE1 protein that is essential for miRNA biogenesis. The present study revealed that dek219 consistently exhibits a stable phenotype characterized by a reduced kernel length. Further analysis indicated that dek219 may reduce the kernel length by inhibiting the expression of genes involved in regulating kernel length. By employing miRNA-target gene prediction, expression analysis, and correlation analysis, we successfully identified nine transcription factors that potentially participate in the regulation of kernel length under the control of DEK219. Among them, the upregulation fold change of HEAT SHOCK TRANSCRIPTION FACTOR17 (HSF17) expression was the highest, and the difference was most significant. The results of transient expression analysis and electrophoretic mobility shift assay (EMSA) indicated that HSF17 can inhibit the expression of DEFECTIVE ENDOSPERM18 (DE18), a gene involved in regulating kernel length. Furthermore, the hsf17 mutant exhibited a significant increase in kernel length, suggesting that HSF17 functions as a negative regulator of kernel length. The results of this study provide crucial evidence for further elucidating the molecular regulatory mechanism underlying maize kernel length and also offer valuable genetic resources for breeding high-yielding maize varieties.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2223-7747/13/12/1592Test; https://doaj.org/toc/2223-7747Test

الوصول الحر: https://doaj.org/article/babf56a756d54494a9b2ff70e715b035Test

المؤلفون: Qing Zhao, Tongyue Duan, Anqun Chen, Liyu He, Hong Liu, Shuguang Yuan, Chengyuan Tang, Yinghong Liu, Yu Liu, Fuyou Liu, Li Xiao, Xuejing Zhu, Lin Sun

المصدر: Annals of Medicine, Vol 55, Iss 1 (2023)

مصطلحات موضوعية: Bowman’s capsule rupture, clinical manifestations, Oxford MEST-C classification, prognosis, immunoglobulin A vasculitis nephritis, Medicine

الوصف: AbstractObjective Bowman’s capsule rupture (BCR) is a glomerular pathological change, but it is still not well recognized in immunoglobulin A vasculitis nephritis (IgAV-N). The Oxford MEST-C score is a classification for IgA nephropathy; however, its clinical correlation and prognostic value in adult patients with IgAV-N are unclear.Methods A retrospective study of 145 adult patients with IgAV-N diagnosed by renal biopsy was conducted. Clinical manifestations, pathological changes and the prognosis of IgAV-N patients were compared depending on the presence or absence of BCR, International Study of Kidney Disease in Children (ISKDC) classification and MEST-C score. The primary endpoint events were end-stage renal disease, renal replacement therapy and all-cause death.Results In total, 51 of 145 (35.17%) patients with IgAV-N presented with BCR. Patients with BCR had more proteinuria, lower serum albumin, and more crescents. Compared with IgAV-N patients with crescents only, 51/100 patients with crescents combined with BCR had a higher proportion of crescents in all glomeruli (15.79% vs. 9.09%; p = 0.003). Patients with higher ISKDC grades had more severe clinical presentation, but it did not reflect the prognosis. However, the MEST-C score not only reflected clinical manifestations but also predicted prognosis (p

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/0785-3890Test; https://doaj.org/toc/1365-2060Test

الوصول الحر: https://doaj.org/article/945542ababf34206af0e632d0c78a2c9Test

المؤلفون: Jin’e Li, Yinghong Liu, Jianping Liu

المصدر: Frontiers in Physiology, Vol 14 (2023)

مصطلحات موضوعية: peritoneal dialysis, peritoneal fibrosis, EMT, TGF-β1, mechanism, Physiology, QP1-981

الوصف: Peritoneal dialysis (PD) is an effective alternative treatment for patients with end-stage renal disease (ESRD) and is increasingly being adopted and promoted worldwide. However, as the duration of peritoneal dialysis extends, it can expose problems with dialysis inadequacy and ultrafiltration failure. The exact mechanism and aetiology of ultrafiltration failure have been of great concern, with triggers such as biological incompatibility of peritoneal dialysis solutions, uraemia toxins, and recurrent intraperitoneal inflammation initiating multiple pathways that regulate the release of various cytokines, promote the transcription of fibrosis-related genes, and deposit extracellular matrix. As a result, peritoneal fibrosis occurs. Exploring the pathogenic factors and molecular mechanisms can help us prevent peritoneal fibrosis and prolong the duration of Peritoneal dialysis.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fphys.2023.1220450/fullTest; https://doaj.org/toc/1664-042XTest

الوصول الحر: https://doaj.org/article/3b69569425f7464ba701d6f1dafeedbdTest

المؤلفون: Ning Zhuo, Gang Wang, Panai Song, Yao Liu, Shuqing Li, Yinghong Liu

المصدر: Renal Failure, Vol 44, Iss 1, Pp 2006-2009 (2022)

مصطلحات موضوعية: Diseases of the genitourinary system. Urology, RC870-923

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/0886-022XTest; https://doaj.org/toc/1525-6049Test

الوصول الحر: https://doaj.org/article/d47b618e768c47a98bfa14aeb86f3f38Test

المؤلفون: Xiaoyan Li, Qingwei Zhang, Songtao Hong, Yinghong Liu

المصدر: Kuangchan zonghe liyong, Vol 43, Iss 4, Pp 6-11 (2022)

مصطلحات موضوعية: lead-zinc mine, heavy metals, release migration, tailings, Mining engineering. Metallurgy, TN1-997

الوصف: The dumping of lead-zinc tailings causes serious environmental pollution around the mine. In order to study the safe dumping of tailings and the removal of a certain amount of heavy metals during the dumping process, the release and migration of Zn, Cd, Pb, Cu and As under different oxidant concentrations and different liquid to solid ratios were investigated in lead-zinc tailings of Lutang, Dachang, Guangxi. The results show that the oxidizing environment can effectively promote the release and migration of the elements. The higher the oxidant concentration, the more the elements can be migrated out. The optimum liquid-solid ratio for release and migration varies among the elements, with the most suitable solid to liquid ratio being 1∶10 for Zn and 1∶6 for Cd, 1∶6 for Pb, 1∶5 for Cu and 1∶6 for As.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1000-6532Test

الوصول الحر: https://doaj.org/article/def4f8a0f1064546a430f5a9c1252767Test

المؤلفون: Ran Tian, Sidi Xie, Junjie Zhang, Hanmei Liu, Yangping Li, Yufeng Hu, Yubi Huang, Yinghong Liu

المصدر: Plants, Vol 12, Iss 20, p 3639 (2023)

مصطلحات موضوعية: maize, MOR signaling network, cold stress, cold tolerance, Botany, QK1-989

الوصف: The MOR (Morphogenesis-related NDR kinase) signaling network, initially identified in yeast, exhibits evolutionary conservation across eukaryotes and plays indispensable roles in the normal growth and development of these organisms. However, the functional role of this network and its associated genes in maize (Zea mays) has remained elusive until now. In this study, we identified a total of 19 maize MOR signaling network genes, and subsequent co-expression analysis revealed that 12 of these genes exhibited stronger associations with each other, suggesting their potential collective regulation of maize growth and development. Further analysis revealed significant co-expression between genes involved in the MOR signaling network and several genes related to cold tolerance. All MOR signaling network genes exhibited significant co-expression with COLD1 (Chilling tolerance divergence1), a pivotal gene involved in the perception of cold stimuli, suggesting that COLD1 may directly transmit cold stress signals to MOR signaling network genes subsequent to the detection of a cold stimulus. The findings indicated that the MOR signaling network may play a crucial role in modulating cold tolerance in maize by establishing an intricate relationship with key cold tolerance genes, such as COLD1. Under low-temperature stress, the expression levels of certain MOR signaling network genes were influenced, with a significant up-regulation observed in Zm00001d010720 and a notable down-regulation observed in Zm00001d049496, indicating that cold stress regulated the MOR signaling network. We identified and analyzed a mutant of Zm00001d010720, which showed a higher sensitivity to cold stress, thereby implicating its involvement in the regulation of cold stress in maize. These findings suggested that the relevant components of the MOR signaling network are also conserved in maize and this signaling network plays a vital role in modulating the cold tolerance of maize. This study offered valuable genetic resources for enhancing the cold tolerance of maize.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2223-7747/12/20/3639Test; https://doaj.org/toc/2223-7747Test

الوصول الحر: https://doaj.org/article/080f5d3bb4d240ca88ce7969bbdb7977Test

المؤلفون: Kun Hu, Qiao Dai, Babatope Samuel Ajayo, Hao Wang, Yufeng Hu, Yangping Li, Huanhuan Huang, Hanmei Liu, Yinghong Liu, Yayun Wang, Lei Gao, Ying Xie

مصطلحات موضوعية: Genetics, Genome-wide identification, Wall associated kinase, Gibberellic acid, Transcriptional factor, Maize Kernel

الوصف: Additional file 1: Fig. S1.

العلاقة: https://figshare.com/articles/journal_contribution/Additional_file_1_of_Insights_into_ZmWAKL_in_maize_kernel_development_genome-wide_investigation_and_GA-mediated_transcription/24792333Test

الإتاحة: https://doi.org/10.6084/m9.figshare.24792333.v1Test
https://figshare.com/articles/journal_contribution/Additional_file_1_of_Insights_into_ZmWAKL_in_maize_kernel_development_genome-wide_investigation_and_GA-mediated_transcription/24792333Test

المؤلفون: Ying Li, Yonghong Xu, Shunjiao Wu, Baohe Wang, Yaying Li, Yinghong Liu, Jia Wang

المصدر: Insects; Volume 14; Issue 8; Pages: 671

مصطلحات موضوعية: Bactrocera dorsalis, validamycin, trehalose, trehalase, trehalose-6-phosphate phosphatase, chitin, chitinase, imaginal disc growth factor

جغرافية الموضوع: agris

الوصف: The oriental fruit fly, Bactrocera dorsalis (Hendel), is a notorious invasive pest that has raised concerns worldwide. Validamycin has been demonstrated to be a very strong inhibitor against trehalase in a variety of organisms. However, whether validamycin can inhibit trehalase activity to suppress trehalose hydrolysis and affect any other relevant physiological pathways in B. dorsalis remains unknown. In this study, the effects of validamycin injection on the synthesis and metabolism of trehalose and chitin were evaluated. The results show that validamycin injection significantly affected trehalase activity and caused trehalose accumulation. In addition, the downstream pathways of trehalose hydrolysis, including the synthesis and metabolism of chitin, were also remarkably affected as the expressions of the key genes in these pathways were significantly regulated and the chitin contents were changed accordingly. Intriguingly, the upstream trehalose synthesis was also affected by validamycin injection due to the variations in the expression levels of key genes, especially BdTPPC1. Moreover, BdTPPC1 was predicted to have a binding affinity to validamycin, and the subsequent in vitro recombinant enzyme activity assay verified the inhibitory effect of validamycin on BdTPPC1 activity for the first time. These findings collectively indicate that validamycin can be considered as a promising potential insecticide for the management of B. dorsalis.

وصف الملف: application/pdf

العلاقة: Insect Physiology, Reproduction and Development; https://dx.doi.org/10.3390/insects14080671Test

الإتاحة: https://doi.org/10.3390/insects14080671Test

المؤلفون: Yuanfen Gao, Xuewu He, Huayang Lv, Hanmei Liu, Yangping Li, Yufeng Hu, Yinghong Liu, Yubi Huang, Junjie Zhang

المصدر: International Journal of Molecular Sciences; Volume 24; Issue 5; Pages: 4614

مصطلحات موضوعية: bHLH157, C4 NADP-ME, epi-brassinolide, NF-YC2, photosynthesis

جغرافية الموضوع: agris

الوصف: Maize is a main food and feed crop with great production potential and high economic benefits. Improving its photosynthesis efficiency is crucial for increasing yield. Maize photosynthesis occurs mainly through the C4 pathway, and NADP-ME (NADP-malic enzyme) is a key enzyme in the photosynthetic carbon assimilation pathway of C4 plants. ZmC4-NADP-ME catalyzes the release of CO2 from oxaloacetate into the Calvin cycle in the maize bundle sheath. Brassinosteroid (BL) can improve photosynthesis; however, its molecular mechanism of action remains unclear. In this study, transcriptome sequencing of maize seedlings treated with epi-brassinolide (EBL) showed that differentially expressed genes (DEGs) were significantly enriched in photosynthetic antenna proteins, porphyrin and chlorophyll metabolism, and photosynthesis pathways. The DEGs of C4-NADP-ME and pyruvate phosphate dikinase in the C4 pathway were significantly enriched in EBL treatment. Co-expression analysis showed that the transcription level of ZmNF-YC2 and ZmbHLH157 transcription factors was increased under EBL treatment and moderately positively correlated with ZmC4-NADP-ME. Transient overexpression of protoplasts revealed that ZmNF-YC2 and ZmbHLH157 activate C4-NADP-ME promoters. Further experiments showed ZmNF-YC2 and ZmbHLH157 transcription factor binding sites on the −1616 bp and −1118 bp ZmC4 NADP-ME promoter. ZmNF-YC2 and ZmbHLH157 were screened as candidate transcription factors mediating brassinosteroid hormone regulation of the ZmC4 NADP-ME gene. The results provide a theoretical basis for improving maize yield using BR hormones.

وصف الملف: application/pdf

العلاقة: Molecular Plant Sciences; https://dx.doi.org/10.3390/ijms24054614Test

الإتاحة: https://doi.org/10.3390/ijms24054614Test