المؤلفون: Yongrong Lei, Xishu Wang, Heng Sun, Yuna Fu, Yichen Tian, Ludi Yang, Jianhua Wang, Feng Xia

المصدر: Frontiers in Oncology, Vol 14 (2024)

مصطلحات موضوعية: recurrence, cancer stem cells, hepatocellular carcinoma, circulating tumor cells, epithelial-mesenchymal, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fonc.2024.1438731/fullTest; https://doaj.org/toc/2234-943XTest

الوصول الحر: https://doaj.org/article/f2b1bdf12a8d4faf97bcbaec5e7de656Test

المؤلفون: Zhaoli Luo, Ziyang Sheng, Liye Hu, Lei Shi, Yichen Tian, Xiaochu Zhao, Wei Yang, Zhongnan Xiao, Danmin Shen, Weihua Wu, Ting Lan, Boqian Zhao, Xiaogang Wang, Nan Zhuang, Jian-Nan Zhang, Yamei Wang, Yabin Lu, Liyong Wang, Chenguang Zhang, Peipei Wang, Jing An, Fei Yang, Qian Li

المصدر: EBioMedicine, Vol 101, Iss , Pp 104993- (2024)

مصطلحات موضوعية: Irg1, Itaconate, Macrophage, Phagocytosis, Intracerebral hemorrhagic stroke, Peritonitis, Medicine, Medicine (General), R5-920

الوصف: Summary: Background: Macrophages are innate immune cells whose phagocytosis function is critical to the prognosis of stroke and peritonitis. cis-aconitic decarboxylase immune-responsive gene 1 (Irg1) and its metabolic product itaconate inhibit bacterial infection, intracellular viral replication, and inflammation in macrophages. Here we explore whether itaconate regulates phagocytosis. Methods: Phagocytosis of macrophages was investigated by time-lapse video recording, flow cytometry, and immunofluorescence staining in macrophage/microglia cultures isolated from mouse tissue. Unbiased RNA-sequencing and ChIP-sequencing assays were used to explore the underlying mechanisms. The effects of Irg1/itaconate axis on the prognosis of intracerebral hemorrhagic stroke (ICH) and peritonitis was observed in transgenic (Irg1flox/flox; Cx3cr1creERT/+, cKO) mice or control mice in vivo. Findings: In a mouse model of ICH, depletion of Irg1 in macrophage/microglia decreased its phagocytosis of erythrocytes, thereby exacerbating outcomes (n = 10 animals/group, p

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2352396424000288Test; https://doaj.org/toc/2352-3964Test

الوصول الحر: https://doaj.org/article/c3cd632bd5234fd4841628e58edfc69bTest

المؤلفون: Guodong Zhou, Jiashuo Tian, Yichen Tian, Qifeng Ma, Quan Li, Shifeng Wang, Huoying Shi

المصدر: BMC Veterinary Research, Vol 19, Iss 1, Pp 1-13 (2023)

مصطلحات موضوعية: Recombinant attenuated Salmonella vaccine, P. multocida, PlpE, Vaccine candidate, Immune responses, Veterinary medicine, SF600-1100

الوصف: Abstract Background Bacterial surface proteins play key roles in pathogenicity and often contribute to microbial adhesion and invasion. Pasteurella lipoprotein E (PlpE), a Pasteurella multocida (P. multocida) surface protein, has recently been identified as a potential vaccine candidate. Live attenuated Salmonella strains have a number of potential advantages as vaccine vectors, including immunization with live vector can mimic natural infections by organisms, lead to the induction of mucosal, humoral, and cellular immune responses. In this study, a previously constructed recombinant attenuated Salmonella Choleraesuis (S. Choleraesuis) vector rSC0016 was used to synthesize and secrete the surface protein PlpE of P. multocida to form the vaccine candidate rSC0016(pS-PlpE). Subsequently, the immunogenicity of S. Choleraesuis rSC0016(pS-PlpE) as an oral vaccine to induce protective immunity against P. multocida in mice was evaluated. Results After immunization, the recombinant attenuated S. Choleraesuis vector can efficiently delivered P. multocida PlpE protein in vivo and induced a specific immune response against this heterologous antigen in mice. In addition, compared with the inactivated vaccine, empty vector (rSC0016(pYA3493)) and PBS immunized groups, the rSC0016(pS-PlpE) vaccine candidate group induced higher antigen-specific mucosal, humoral and mixed Th1/Th2 cellular immune responses. After intraperitoneal challenge, the rSC0016(pS-PlpE) immunized group had a markedly enhanced survival rate (80%), a better protection efficiency than 60% of the inactivated vaccine group, and significantly reduced tissue damage. Conclusions In conclusion, our study found that the rSC0016(pS-PlpE) vaccine candidate provided good protection against challenge with wild-type P. multocida serotype A in a mouse infection model, and may potentially be considered for use as a universal vaccine against multiple serotypes of P. multocida in livestock, including pigs.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1746-6148Test

الوصول الحر: https://doaj.org/article/e2562dc9e89a43deb9be0689df52a9faTest

المؤلفون: Junhua Wang, Yaoting Shi, Jianbo Chen, Juying Liu, Xiaotian Zhao, Jiaohui Pang, Ximin Sun, Yichen Tian, Qiuxiang Ou, Feng Xia, Yunjie Chen

المصدر: Cancer Biology & Therapy, Vol 24, Iss 1 (2023)

مصطلحات موضوعية: genomic profiling, prognosis, patient age, cholangiocarcinoma, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Molecular mechanisms behind potentially inferior prognosis of old cholangiocarcinoma (CCA) patients are unclear. Prevalence of interventional targets and the difference between young and old CCA patients are valuable for promising precision medicine. A total of 188 CCA patients with baseline tumor tissue samples were subgrouped into the young (≤45 years) and old (>45 years) sub-cohorts. Somatic and germline mutation profiles, differentially enriched genetic alterations, and actionable genetic alterations were compared. An external dataset was used for the validation of molecular features and the comparison of overall survival (OS). Compared to young patients, KRAS alterations were more common in old patients (P = .04), while FGFR2 fusions were less frequent (P = .05). TERT promoter mutations were exclusively detected in old patients. The external dataset (N = 392) revealed no significant difference in OS between young and old patients; however, old patient-enriched KRAS (hazard ratio [HR]: 1.96, 95% confidence interval [CI]: 1.37–2.80) and TERT alterations (HR: 2.03, 95% CI: 1.22–3.38) were associated with inferior OS. Approximately 38.3% of patients were identified of actionable oncogenic mutations indicative of a potential response to targeted therapy or immunotherapy. Actionable FGFR2 fusions (P = .01) and BRAFV600E (P = .04) mutations were more frequent in young females than old patients. The enrichment of KRAS/TERT alterations in CCA patients over 45 years resulted in inferior OS. Approximately one-third of CCA patients were eligible for targeted therapy or immunotherapy given the actionable mutations carried, especially young females.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1538-4047Test; https://doaj.org/toc/1555-8576Test

الوصول الحر: https://doaj.org/article/92ec23f2309a4dcb9b7af12de7be515eTest

المؤلفون: YongRong Lei, Fenghui Liao, YiChen Tian, YaNi Wang, Feng Xia, JianHua Wang

المصدر: Frontiers in Neuroscience, Vol 17 (2023)

مصطلحات موضوعية: chronic stress, tumor microenvironment, immunotherapy, anticancer treatments, sympathetic nervous system, neural-immune interactions, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571

الوصف: Chronic stress has a substantial influence on the tumor microenvironment (TME), leading to compromised effectiveness of anti-cancer therapies through diverse mechanisms. It disrupts vital functions of immune cells that play a critical role in anti-tumor immunity, such as the inhibition of dendritic cells (DCs) and lymphocytes, while simultaneously enhancing the activity of immune cells that support tumor growth, such as myeloid-derived suppressor cells and tumor-associated macrophages. Furthermore, chronic stress exerts a significant impact on crucial mechanisms within the TME, including angiogenesis, DNA repair, hypoxia, extracellular matrix deposition, and tumor metabolism. These alterations in the TME, induced by stress, result from the activation of the hypothalamic–pituitary–adrenal axis and sympathetic nervous system, in conjunction with epigenetic modifications. In conclusion, chronic stress significantly influences the TME and impedes the efficacy of anti-cancer treatments, underscoring the importance of targeting stress pathways to improve therapeutic results.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fnins.2023.1321176/fullTest; https://doaj.org/toc/1662-453XTest

الوصول الحر: https://doaj.org/article/9695245e95524b708488bbe5fe058ce6Test

المؤلفون: Xueyao Zhang, Guangxiao Li, Chuning Shi, Yichen Tian, Linlin Zhang, Hongyu Zhang, Yingxian Sun

المصدر: Scientific Reports, Vol 13, Iss 1, Pp 1-12 (2023)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract We aimed to compare the relationship between hypertension and obesity-related anthropometric indices (waist circumference [WC], waist-height ratio, waist-hip ratio [WHR], and body mass index; unconventional: new body shape index [ABSI] and body roundness index [BRI]) to identify best predictors of new-onset hypertension. The study included 4123 adult participants (2377 women). Hazard ratios (HRs) and 95% confidence intervals (CIs) were determined using a Cox regression model to estimate the risk of new-onset hypertension with respect to each obesity index. In addition, we assessed the predictive value of each obesity index for new-onset hypertension using area under the receiver operating characteristic curve (AUC) after adjusting for common risk factors. During the median follow-up of 2.59 years, 818 (19.8%) new hypertension cases were diagnosed. The non-traditional obesity indices BRI and ABSI had predictive value for new-onset hypertension; however, they were not better than the traditional indexes. WHR was the best predictor of new-onset hypertension in women aged ≤ 60 and > 60 years, with HRs of 2.38 and 2.51 and AUCs of 0.793 and 0.716. However, WHR (HR 2.28, AUC = 0.759) and WC (HR 3.24, AUC = 0.788) were the best indexes for predicting new-onset hypertension in men aged ≤ 60 and > 60 years, respectively.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/45e854dc311940fe9d28a3d50e5d1f30Test

المؤلفون: Xinyi Feng, Huiping Huang, Yingqi Wang, Yichen Tian, Liping Li

المصدر: Remote Sensing, Vol 16, Iss 7, p 1258 (2024)

مصطلحات موضوعية: ecological sources, ecosystem services value, vegetation productivity, Three-River Headwaters Region, Science

الوصف: As a crucial component of the ecological security pattern, ecological source (ES) plays a vital role in providing ecosystem service value (ESV) and conserving biodiversity. Previous studies have mostly considered ES only from either landscape change pattern or ecological function perspectives, and have ignored their integration and spatio-temporal evolutionary modeling. In this study, we proposed a multi-perspective framework for the spatio-temporal characteristics of ES by ESV incorporating landscape aesthetics, carbon sink characteristics, ecological quality, and kernel NDVI (kNDVI). By integrating the revised ESV and the kernel normalized difference vegetation index as a foundation, we employed the spatial priority model to identify ES. This improvement aims to yield a more practical and specific ESV result. Applying this framework to the Three-River Headwaters Region (TRHR), a significant spatio-temporal change in ecological sources has been observed from 2000 to 2020. This performance provided a reference for ecological conservation in the TRHR. The results indicate that this ecological source identification framework has reliable accuracy and efficiency compared with the existing NRs in the TRHR. This method could reveal more precise spatio-temporal distributions of ES, enhancing ecosystem integrity and providing technical modeling support for developing cross-scale spatial planning and management strategies for nature reserve boundaries. The framework proposed in our research could serve as a reference for building ecological networks in other ecologically fragile areas.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2072-4292/16/7/1258Test; https://doaj.org/toc/2072-4292Test

الوصول الحر: https://doaj.org/article/6bada4b08c1b46458e75c77dbfeb2a6fTest

المؤلفون: Rihong Wen, Meiou Qin, Peng Jiang, Feiyun Yang, Bin Liu, Mengyuan Zhu, Yuan Fang, Yichen Tian, Bo Shang

المصدر: Atmosphere, Vol 15, Iss 4, p 408 (2024)

مصطلحات موضوعية: vapor pressure deficit, LAI, evapotranspiration, water availability, global forests, Meteorology. Climatology, QC851-999

الوصف: A forest is vulnerable to drought and plays important roles in the regulation of carbon and water cycling in a terrestrial ecosystem. Atmospheric vapor pressure deficit (VPD) has been identified as an increasingly major factor in plant functioning and has been established as a main contributor to recent drought-induced plant mortality, independent of other drivers associated with climate change. However, most previous studies have focused on the effects of climate warming and CO2 enrichment on vegetation growth, without considering the effects of an increased VPD on vegetation growth and evapotranspiration (ET) in forest ecosystems. This could lead to a large uncertainty in estimating the variability in forest carbon sinks. Based on the long-term satellite data, we investigated the response of the leaf area index (LAI) and ET to the VPD via a partial correlation analysis in this study. We also examined the temporal variability in the partial coefficients within a ten-year moving window. The results showed that over 50% of the region displayed a negative partial correlation between the LAI, ET, and VPD, and those pixels were mainly concentrated in North America and the plains of Eastern Europe. Regions with a negative trend of partial correlation in both the LAI and ET are mostly located in the plains of Eastern Europe and the Siberian Plain of western Russia, while the positive trend is mainly in South America. The plains of Eastern Europe are becoming drier, which was proved by the interannual trend of the Standardized Precipitation Evapotranspiration Index (SPEI) and soil water content (SWC). Additionally, the LAI and ET in those areas exhibited a significant positive correlation with the SWC based on the moving window average. This study suggests that the role of the VPD on vegetation will become increasingly prominent in the context of future climate change for the forest ecosystem.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2073-4433/15/4/408Test; https://doaj.org/toc/2073-4433Test

الوصول الحر: https://doaj.org/article/488cb1aab06b421fb6efc626b2ca2a2aTest

المؤلفون: Wenlu Zhu, Chao Yuan, Yichen Tian, Yingqi Wang, Liping Li, Chenlu Hu

المصدر: Remote Sensing, Vol 16, Iss 7, p 1126 (2024)

مصطلحات موضوعية: rural built-up land extraction, Artificial Surface Index, SWIR downscaling, high-resolution remote sensing image, Science

الوصف: The complexity of surface characteristics in rural areas poses challenges for accurate extraction of built-up areas from remote sensing images. The Artificial Surface Index (ASI) emerged as a novel and accurate built-up land index. However, the absence of short-wave infrared (SWIR) bands in most high-resolution (HR) images restricts the application of index-based methods in rural built-up land extraction. This paper presents a rapid extraction method for high-resolution built-up land in rural areas based on ASI. Through the downscaling techniques of random forest (RF) regression, high-resolution SWIR bands were generated. They were then combined with visible and near-infrared (VNIR) bands to compute ASI on GaoFen-2 (GF-2) images (called ASIGF). Furthermore, a red roof index (RRI) was designed to reduce the probability of misclassifying built-up land with bare soil. The results demonstrated that SWIR downscaling effectively compensates for multispectral information absence in HR imagery and expands the applicability of index-based methods to HR remote sensing data. Compared with five other indices (UI, BFLEI, NDBI, BCI, and PISI), the combination of ASI and RRI achieved the optimal performance in built-up land enhancement and bare land suppression, particularly showcasing superior performance in rural built-up land extraction.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2072-4292/16/7/1126Test; https://doaj.org/toc/2072-4292Test

الوصول الحر: https://doaj.org/article/2513ed4f9c11447a881356d416985dccTest

المؤلفون: Heng Sun, Fenghui Liao, Yichen Tian, Yongrong Lei, Yuna Fu, Jianhua Wang

المصدر: International Journal of Molecular Sciences, Vol 25, Iss 5, p 2474 (2024)

مصطلحات موضوعية: atomic force microscopy, protein–protein interactions, BAX/Bcl-2, polyphenols, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: Apoptosis signaling controls the cell cycle through the protein–protein interactions (PPIs) of its major B-cell lymphoma 2-associated x protein (BAX) and B-cell lymphoma 2 protein (Bcl-2). Due to the antagonistic function of both proteins, apoptosis depends on a properly tuned balance of the kinetics of BAX and Bcl-2 activities. The utilization of natural polyphenols to regulate the binding process of PPIs is feasible. However, the mechanism of this modulation has not been studied in detail. Here, we utilized atomic force microscopy (AFM) to evaluate the effects of polyphenols (kaempferol, quercetin, dihydromyricetin, baicalin, curcumin, rutin, epigallocatechin gallate, and gossypol) on the BAX/Bcl-2 binding mechanism. We demonstrated at the molecular scale that polyphenols quantitatively affect the interaction forces, kinetics, thermodynamics, and structural properties of BAX/Bcl-2 complex formation. We observed that rutin, epigallocatechin gallate, and baicalin reduced the binding affinity of BAX/Bcl-2 by an order of magnitude. Combined with surface free energy and molecular docking, the results revealed that polyphenols are driven by multiple forces that affect the orientation freedom of PPIs, with hydrogen bonding, hydrophobic interactions, and van der Waals forces being the major contributors. Overall, our work provides valuable insights into how molecules tune PPIs to modulate their function.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1422-0067/25/5/2474Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/4671f102e4cc4a0e8fa85dff0a7c1b49Test