المؤلفون: Mengqing Gu, Yibo Wang, Yang Yu

المصدر: Journal of Ovarian Research, Vol 17, Iss 1, Pp 1-12 (2024)

مصطلحات موضوعية: Ovarian fibrosis, Ovarian dysfunction, Ovarian aging, Assisted Reproductive Technology (ART), Therapeutic targets, Gynecology and obstetrics, RG1-991

الوصف: Abstract Ovarian fibrosis, characterized by the excessive proliferation of ovarian fibroblasts and the accumulation of extracellular matrix (ECM), serves as one of the primary causes of ovarian dysfunction. Despite the critical role of ovarian fibrosis in maintaining the normal physiological function of the mammalian ovaries, research on this condition has been greatly underestimated, which leads to a lack of clinical treatment options for ovarian dysfunction caused by fibrosis. This review synthesizes recent research on the molecular mechanisms of ovarian fibrosis, encompassing TGF-β, extracellular matrix, inflammation, and other profibrotic factors contributing to abnormal ovarian fibrosis. Additionally, we summarize current treatment approaches for ovarian dysfunction targeting ovarian fibrosis, including antifibrotic drugs, stem cell transplantation, and exosomal therapies. The purpose of this review is to summarize the research progress on ovarian fibrosis and to propose potential therapeutic strategies targeting ovarian fibrosis for the treatment of ovarian dysfunction.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1757-2215Test

الوصول الحر: https://doaj.org/article/f79ad783fbd84aa9ad56b171f46595d7Test

المؤلفون: Yingdong Han, Hong Di, Yibo Wang, Yun Zhang, Xuejun Zeng

المصدر: BMC Public Health, Vol 24, Iss 1, Pp 1-12 (2024)

مصطلحات موضوعية: Chronic kidney disease, Life’s essential 8, Cardiovascular health, NHANES, All-cause mortality, Public aspects of medicine, RA1-1270

الوصف: Abstract Background People with chronic kidney disease (CKD) are more likely to die prematurely, and this increased risk of death is primarily attributable to deaths from cardiovascular disease (CVD). We aim to investigate the relationship between Life’s Essential 8 (LE8), a newly proposed cardiovascular health (CVH) measurement system, and all-cause mortality of CKD patients among US adults. Methods A total of 3,169 CKD patients aged 20 and older from the National Health and Nutritional Examination Survey in 2009–2016 were involved in this study. Participants were divided into low (0–49), moderate (50–79) and high (80–100) CVH groups according to LE8 score (range 0-100). The mortality was ascertained from the National Death Index. Cox proportional hazards regression and restricted cubic spline were used to investigate the relationship. Results Among the 3,169 CKD patients, the median age was 66.0 (25.0) years and 1,671 (52.7%) were female, and the median follow-up time was 6.00 years. The median LE8 score of the study cohort was 57.5 (19.4). CKD patients with low CVH, health behavior (HB) and health factors (HF) scores presented with higher all-cause mortality (both log-rank P-values

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2458Test

الوصول الحر: https://doaj.org/article/042d18f67d8e410aabd182ae6aee87bdTest

المؤلفون: Qingxia Hu, Junling Yin, Sijie Zhao, Yibo Wang, Ruxue Shi, Keqiang Yan, Shuhong Huang

المصدر: Cell Death Discovery, Vol 10, Iss 1, Pp 1-12 (2024)

مصطلحات موضوعية: Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282, Cytology, QH573-671

الوصف: Abstract Zinc-finger homeobox 3 (ZFHX3, also known as ATBF1) suppresses prostatic tumorigenesis. ZFHX3 is frequently found to have numerous deletions in human prostate cancer (PCa). However, the underlying molecular function of ZFHX3 during prostatic tumorigenesis is not well understood. N 6-methyladenosine (m6A) modification in RNA plays a critical role in the development of cancers; however, the relationship between ZFHX3 and m6A modification is largely unknown in PCa. In this study, we found that ZFHX3 knockdown decreased total m6A levels through enhancing the transcriptional activity of FTO in PCa cells. Importantly, FTO inhibition suppressed cell proliferation and rescued the promoting function of ZFHX3 knockdown on cell proliferation. In vivo, we verified that FTO was upregulated and ZFHX3 was decreased in PCa patients and that a high level of ZFHX3 is indispensable for low FTO expression and is correlated with better patient survival. Through transcriptome sequencing and MeRIP sequencing, we revealed that E2F2 and CDKN2C were the direct targets of FTO-mediated m6A modification and ZFXH3 was required for the regulation of FTO on E2F2 and CDKN2C expression. Unexpectedly, we uncovered that ZFHX3 expression was in return regulated by FTO in an m6A-dependent way. These findings establish a novel crosstalk mechanism between ZFHX3 and FTO in prostatic tumorigenesis.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2058-7716Test

الوصول الحر: https://doaj.org/article/d3d7442a06f748a8a8ef2dd6ac1c5cfbTest

المؤلفون: Siyue Lei, Xiuqin Ma, Hongwu Qin, Yibo Wang, Jasni Mohamad Zain

المصدر: Scientific Reports, Vol 14, Iss 1, Pp 1-25 (2024)

مصطلحات موضوعية: Interval-valued Fermatean hesitant fuzzy sets, Einstein t-norms, Bonferroni mean, Multi-attribute group decision-making, Medicine, Science

الوصف: Abstract Faced with the increasing complexity and uncertainty of decision-making information, interval-valued Fermatean hesitant fuzzy sets (IVFHFSs) were presented as a novel mathematical model that handled uncertain data more effectively. However, existing multi-attribute group decision-making (MAGDM) methods based on IVFHFSs do not thoroughly investigate the operational laws. Also, these existing MAGDM methods do not take into account the connections between attributes and are less flexible. To address these issues, this paper proposes a new MAGDM method based on Einstein Bonferroni operators under IVFHFSs. First, we thoroughly examine the operational laws of Einstein t-norms under the IVFHFSs to further extend the study of the operational laws. Then, we introduce the interval-valued Fermatean hesitant fuzzy Einstein Bonferroni mean operator and the interval-valued Fermatean hesitant fuzzy Einstein weighted Bonferroni mean operator under Einstein t-norms. Our suggested aggregation operators consider the relationship between attributes and are far more flexible in comparison to the current approaches. Later, a novel MAGDM method based on Einstein Bonferroni operators under the IVFHFSs is given. Finally, the practicality and validity of the proposed method are demonstrated by a cardiovascular disease diagnosis application.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/d7990a69ac4b4b38a1dd9afb490bfb1cTest

المؤلفون: Wendi Pei, Lin Fu, Wenhuan Guo, Yibo Wang, Yong Fan, Rui Yang, Rong Li, Jie Qiao, Yang Yu

المصدر: Stem Cell Research & Therapy, Vol 15, Iss 1, Pp 1-16 (2024)

مصطلحات موضوعية: Mesenchymal stem cell, Ovary, Ageing, Mouse model, Safety, Medicine (General), R5-920, Biochemistry, QD415-436

الوصف: Abstract Background Ovarian ageing is one of the major issues that impacts female fertility. Mesenchymal stem cell (MSC)-based therapy has made impressive progress in recent years. However, the efficacy and safety of MSCs, as nonautologous components, remain to be further verified. Methods Two common sources of MSCs, umbilical cord-derived MSCs (UC-MSCs) and adipose tissue-derived MSCs (AD-MSCs), were orthotopically transplanted into a mouse model of ovarian ageing to evaluate their therapeutic effects. The safety of the treatment was further evaluated, and RNA sequencing was performed to explore the underlying mechanisms involved. Results After orthotopic transplantation of MSCs into the ovary, the oestrous cycle, ovarian weight, number and proportion of primary follicles, granulosa cell proliferation, and angiogenesis were improved. The effects of AD-MSCs were superior to those of UC-MSCs in several indices, such as post-transplant granulosa cell proliferation, ovarian weight and angiogenesis. Moreover, the tumorigenesis, acute toxicity, immunogenicity and biodistribution of MSCs were evaluated, and both AD-MSCs and UC-MSCs were found to possess high safety profiles. Through RNA sequencing analysis, enhancement of the MAPK cascade was observed, and long-term effects were mainly linked to the activation of immune function. Conclusions Orthotopic transplantation of MSCs displays significant efficacy and high safety for the treatment of ovarian ageing in mice.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1757-6512Test

الوصول الحر: https://doaj.org/article/1f8ac20cad814ab8bf29271649f0b3c2Test

المؤلفون: Annan Ming, Jianxin Zhao, Yihan Liu, Yibo Wang, Xiaohui Wang, Jing Li, Leiliang Zhang

المصدر: mLife, Vol 3, Iss 1, Pp 57-73 (2024)

مصطلحات موضوعية: immune response, O‐GalNAc, O‐GlcNAc, O‐glycosylation, virus, Microbiology, QR1-502

الوصف: Abstract O‐glycosylation is an ancient yet underappreciated protein posttranslational modification, on which many bacteria and viruses heavily rely to perform critical biological functions involved in numerous infectious diseases or even cancer. But due to the innate complexity of O‐glycosylation, research techniques have been limited to study its exact role in viral attachment and entry, assembly and exit, spreading in the host cells, and the innate and adaptive immunity of the host. Recently, the advent of many newly developed methodologies (e.g., mass spectrometry, chemical biology tools, and molecular dynamics simulations) has renewed and rekindled the interest in viral‐related O‐glycosylation in both viral proteins and host cells, which is further fueled by the COVID‐19 pandemic. In this review, we summarize recent advances in viral‐related O‐glycosylation, with a particular emphasis on the mucin‐type O‐linked α‐N‐acetylgalactosamine (O‐GalNAc) on viral proteins and the intracellular O‐linked β‐N‐acetylglucosamine (O‐GlcNAc) modifications on host proteins. We hope to provide valuable insights into the development of antiviral reagents or vaccines for better prevention or treatment of infectious diseases.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2770-100XTest

الوصول الحر: https://doaj.org/article/a3a9b5976da141c7a4c74d36859a49aeTest

المؤلفون: Dianhao Guo, Xiaokang Li, Jing Wang, Xin Liu, Yibo Wang, Shuhong Huang, Ningning Dang

المصدر: Cell Death and Disease, Vol 15, Iss 3, Pp 1-14 (2024)

مصطلحات موضوعية: Cytology, QH573-671

الوصف: Abstract The pathogenesis of psoriasis, a chronic inflammatory autoimmune skin disease with a high global prevalence, remains unclear. We performed a high-resolution single-cell RNA sequencing analysis of 94,759 cells from 13 samples, including those from psoriasis model mice and wild-type mice. We presented a single-cell atlas of the skin of imiquimod-induced mice with psoriasis and WT mice, especially the heterogeneity of keratinocytes and fibroblasts. More interestingly, we discovered that special keratinocyte subtypes and fibroblast subtypes could interact with each other through epithelial–mesenchymal transition and validated the results with drug verification. Moreover, we conducted a tentative exploration of the potential pathways involved and revealed that the IL-17 signalling pathway may be the most relevant pathway. Collectively, we revealed the full-cycle landscape of key cells associated with psoriasis and provided a more comprehensive understanding of the pathogenesis of psoriasis.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2041-4889Test

الوصول الحر: https://doaj.org/article/635b166fc87246fbbcbd5e5c42d8bf82Test

المؤلفون: Yibo Wang, Xiaona Huang, Yan Liu, Xiangyu Zhang, Bing Yang, Yanan Yue

المصدر: Energy Storage and Saving, Vol 3, Iss 1, Pp 52-59 (2024)

مصطلحات موضوعية: High-entropy alloys, Thin coating, Thermal conductivity, Mechanical properties, Noncontact photothermal technique, Energy conservation, TJ163.26-163.5

الوصف: High-entropy alloys (HEAs) exhibit extraordinary physical properties such as superior strength-to-weight ratios and enhanced corrosion and oxidation resistance, making them potentially useful in energy storage and generation industries. However, thermal and mechanical properties of HEAs with various compositions vary significantly. Furthermore, these properties have rarely been investigated simultaneously owing to material or instrumentation limitations. Herein, we synthesize an HEA (AlCrNbSiTi) coating with a thickness of less than 2 μm. We customize a frequency-domain photothermal testing system to characterize the thermal and mechanical properties of the proposed coating with high accuracy. Owing to the large mixing enthalpy of the Al-Ti, Nb-Si, and Ti-Si pairs in the coating, its hardness and elastic modulus are 15.2 and 254.7 GPa, respectively which are higher than those of previously reported HEAs. The thermal conductivity of the AlCrNbSiTi coating is characterized to be 2.90 W·m−1·K−1, within the expected range and well explained by the free-electron consistency diversity and phonon scattering from the amorphous structure. Additionally, the coating exhibits adequate wear performance, with a wear rate of 5.4 × 10−8 mm3·N−1·m−1. This relatively low thermal conductivity, combined with extraordinary mechanical properties, makes the proposed material an excellent candidate as a protective coating material for nuclear reactor components which require high strength, irradiation resistance, and thermal protection.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2772683523000316Test; https://doaj.org/toc/2772-6835Test

الوصول الحر: https://doaj.org/article/7c5df9f531e44b328cd9b2005f8e2ad0Test

المؤلفون: Yu Chen, Lizhou Ma, Yibo Wang, Jiarui Zhang, Tianhe Pei, Miao Wang

المصدر: Frontiers in Pharmacology, Vol 15 (2024)

مصطلحات موضوعية: proteomics, liver injury, gypenosides, label-free, parallel reaction monitoring, Therapeutics. Pharmacology, RM1-950

الوصف: Chronic liver disease, a long-term condition resulting from various causes such as alcohol abuse, metabolic disorders, and viral hepatitis, is becoming a significant global health challenge. Gypenosides (GPs), derived from the traditional Chinese medicine Gynostemma pentaphyllum (Thunb.) Makino, exhibited hepatoprotective properties in recent years, yet the precise therapeutic mechanism remains unclear. In this study, label-free and parallel reaction monitoring (PRM) proteomics were used to elucidate the hepatoprotective mechanism of GPs in liver injury rats. Through label-free proteomics, we identified 2104 differentially expressed proteins (DEPs) associated with liver injury, along with 1974 DEPs related to the effects of GPs. Bioinformatics analysis revealed that GPs primarily restored metabolic processes involving valine, leucine, and isoleucine degradation, as well as propanoate and butanoate metabolism, and steroid hormone biosynthesis during liver injury. Subsequently, overlapping the two groups of DEPs identified 1508 proteins reversed following GPs treatment, with key targets further validated by PRM. Eight target proteins were identified for GPs treatment of liver injury, including Lgals3, Psat1, Phgdh, Cyp3a9, Cyp2c11, Cyp4a2, Glul, and Ces1d. These findings not only elucidated the hepatoprotective mechanism of GPs, but may also serve as potential therapeutic targets of chronic liver disease.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fphar.2024.1417575/fullTest; https://doaj.org/toc/1663-9812Test

الوصول الحر: https://doaj.org/article/22643f2db9ac4d688075588017b7ce63Test

المؤلفون: Taotao Du, Xudong Qu, Yibo Wang, Meixuan Li, Xihu Qie, Jing Jin, Yuxuan Gao, Zengyu Wang, Kejian Lin, Chao Yang, Juan Sun

المصدر: Frontiers in Microbiology, Vol 15 (2024)

مصطلحات موضوعية: Digitaria sanguinalis, Mortierella, inhibition mechanism, plant hormone, Mortierella strain MXBP304, Microbiology, QR1-502

الوصف: IntroductionWeeds are significant factors that detrimentally affect crop health and hinder optimal herbage yield. Rhizosphere microorganisms play crucial roles in plant growth, development, and nutrient uptake. Therefore, research focusing on weed control through the lens of microorganisms has emerged as a prominent area of study. The oil-producing fungus Mortierella, which is known for its numerous agricultural benefits, has garnered significant attention in recent years.MethodsIn this study, we conducted inoculation experiments in a controlled artificial culture climate chamber to investigate the effects of differential hormones and differentially expressed genes in the stems and leaves of Digitaria sanguinalis using Liquid Chromatography Tandem Mass Spectrometry and RNA-seq techniques, respectively. Additionally, Pearson’s correlation analysis was used to establish correlations between differential hormones and growth indicators of Digitaria sanguinalis.Results and discussionThe results demonstrated that inoculation with Mortierella sp. MXBP304 effectively suppressed aboveground biomass and plant height in Digitaria sanguinalis. Furthermore, there was significant upregulation and downregulation in the expression of genes involved in the synthesis and metabolism of phenylalanine and L-phenylalanine. Conversely, the expression of genes related to tryptophan, L-tryptophan, and indole was significantly downregulated. The addition of Mortierella sp. MXBP304 can influence the gene expression associated with phenylalanine and tryptophan synthesis and metabolism during Digitaria sanguinalis growth, subsequently reducing the relative contents of phenylalanine and tryptophan, thereby directly inhibiting Digitaria sanguinalis growth.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fmicb.2024.1385992/fullTest; https://doaj.org/toc/1664-302XTest

الوصول الحر: https://doaj.org/article/2c752dc8e5254d538633233df942b787Test