المؤلفون: Mathilde Bergamelli, Hélène Martin, Mélinda Bénard, Jérôme Ausseil, Jean-Michel Mansuy, Ilse Hurbain, Maïlys Mouysset, Marion Groussolles, Géraldine Cartron, Yann Tanguy le Gac, Nathalie Moinard, Elsa Suberbielle, Jacques Izopet, Charlotte Tscherning, Graça Raposo, Daniel Gonzalez-Dunia, Gisela D’Angelo, Cécile E. Malnou

المصدر: Frontiers in Cell and Developmental Biology, Vol 9 (2021)

مصطلحات موضوعية: early placenta, extracellular vesicles, congenital infection, human cytomegalovirus, placental histoculture, Biology (General), QH301-705.5

الوصف: Extracellular vesicles (EVs) have increasingly been recognized as key players in a wide variety of physiological and pathological contexts, including during pregnancy. Notably, EVs appear both as possible biomarkers and as mediators involved in the communication of the placenta with the maternal and fetal sides. A better understanding of the physiological and pathological roles of EVs strongly depends on the development of adequate and reliable study models, specifically at the beginning of pregnancy where many adverse pregnancy outcomes have their origin. In this study, we describe the isolation of small EVs from a histoculture model of first trimester placental explants in normal conditions as well as upon infection by human cytomegalovirus. Using bead-based multiplex cytometry and electron microscopy combined with biochemical approaches, we characterized these small EVs and defined their associated markers and ultrastructure. We observed that infection led to changes in the expression level of several surface markers, without affecting the secretion and integrity of small EVs. Our findings lay the foundation for studying the functional role of EVs during early pregnancy, along with the identification of new predictive biomarkers for the severity and outcome of this congenital infection, which are still sorely lacking.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fcell.2021.689122/fullTest; https://doaj.org/toc/2296-634XTest

الوصول الحر: https://doaj.org/article/3c6c7a0436584794b1360c2aaacebf18Test

المؤلفون: Mathilde Bergamelli, Hélène Martin, Yann Aubert, Jean-Michel Mansuy, Marlène Marcellin, Odile Burlet-Schiltz, Ilse Hurbain, Graça Raposo, Jacques Izopet, Thierry Fournier, Alexandra Benchoua, Mélinda Bénard, Marion Groussolles, Géraldine Cartron, Yann Tanguy Le Gac, Nathalie Moinard, Gisela D’Angelo, Cécile E. Malnou

المصدر: Viruses, Vol 14, Iss 9, p 2030 (2022)

مصطلحات موضوعية: hCMV, congenital infection, extracellular vesicles, placenta, cytotrophoblast, Microbiology, QR1-502

الوصف: Although placental small extracellular vesicles (sEVs) are extensively studied in the context of pregnancy, little is known about their role during viral congenital infection, especially at the beginning of pregnancy. In this study, we examined the consequences of human cytomegalovirus (hCMV) infection on sEVs production, composition, and function using an immortalized human cytotrophoblast cell line derived from first trimester placenta. By combining complementary approaches of biochemistry, electron microscopy, and quantitative proteomic analysis, we showed that hCMV infection increases the yield of sEVs produced by cytotrophoblasts and modifies their protein content towards a potential proviral phenotype. We further demonstrate that sEVs secreted by hCMV-infected cytotrophoblasts potentiate infection in naive recipient cells of fetal origin, including human neural stem cells. Importantly, these functional consequences are also observed with sEVs prepared from an ex vivo model of infected histocultures from early placenta. Based on these findings, we propose that placental sEVs could be important actors favoring viral dissemination to the fetal brain during hCMV congenital infection.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1999-4915/14/9/2030Test; https://doaj.org/toc/1999-4915Test

الوصول الحر: https://doaj.org/article/f5ff63c22f6a4b89b933525b3f9e98deTest

المؤلفون: Hélène Leray, Laurent Brouchet, Yann Tanguy Le Gac, Sihem Bouharaoua, Philippe Otal, Gwenaël Ferron, Erwan Gabiache, Martina Aida Angeles, Carlos Martínez-Gómez, Alejandra Martinez

المصدر: Gynecologic Oncology Reports, Vol 36, Iss , Pp 100727- (2021)

مصطلحات موضوعية: Lymph node excision, Diaphragmatic hernia, Chylothorax, Cytoreductive surgical procedures, Postoperative complications, Gynecology and obstetrics, RG1-991, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Resection of enlarged cardiophrenic lymph nodes (CPLN) is a procedure required to obtain complete cytoreduction in selected patients affected by advanced ovarian cancer. Their resection by transdiaphragmatic approach has been demonstrated to be feasible with low rates of morbidity. The main complications associated with this procedure are pleural effusion, pneumothorax, and rarely, chylothorax.This case describes a postoperative chylothorax and chest liver herniation in a patient who underwent a cytoreductive surgery for advanced endometrioid ovarian cancer, which included a right transdiaphragmatic CPLN resection. Surgical management by thoracotomy was required to repair the right diaphragmatic defect combined with conservative management of the chylothorax. The diaphragmatic closure was achieved employing interrupted stitches with a non-absorbable suture. No prosthetic material was required.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2352578921000321Test; https://doaj.org/toc/2352-5789Test

الوصول الحر: https://doaj.org/article/f1126c196cd7446a9f9f3e5d0e3112c4Test

المؤلفون: Hélène Martin, Jonathan Barthelemy, Yamileth Chin, Mathilde Bergamelli, Nathalie Moinard, Géraldine Cartron, Yann Tanguy Le Gac, Cécile E. Malnou, Yannick Simonin

المصدر: Viruses, Vol 14, Iss 8, p 1619 (2022)

مصطلحات موضوعية: arbovirus, flavivirus, Usutu virus, placenta, vertical transmission, Microbiology, QR1-502

الوصف: Usutu virus (USUV) is a neurotropic mosquito-borne flavivirus that has dispersed quickly in Europe these past years. This arbovirus mainly follows an enzootic cycle involving mosquitoes and birds, but can also infect other mammals, causing notably sporadic cases in humans. Although it is mainly asymptomatic or responsible for mild clinical symptoms, USUV has been associated with neurological disorders, such as encephalitis and meningoencephalitis, highlighting the potential health threat of this virus. Among the different transmission routes described for other flaviviruses, the capacity for some of them to be transmitted vertically has been demonstrated, notably for Zika virus or West Nile virus, which are closely related to USUV. To evaluate the ability of USUV to replicate in the placenta and gain access to the fetus, we combined the use of several trophoblast model cell lines, ex vivo human placental explant cultures from first and third trimester of pregnancy, and in vivo USUV-infected pregnant mice. Our data demonstrate that human placental cells and tissues are permissive to USUV replication, and suggest that viral transmission can occur in mice during gestation. Hence, our observations suggest that USUV could be efficiently transmitted by the vertical route.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1999-4915/14/8/1619Test; https://doaj.org/toc/1999-4915Test

الوصول الحر: https://doaj.org/article/9fb517edf15d4e59923a7c7d56f33528Test

المؤلفون: Ali Ghassani, Benoit Andre, Caroline Simon-Toulza, Yann Tanguy le Gac, Alejandra Martinez, Fabien Vidal

المصدر: Case Reports in Obstetrics and Gynecology, Vol 2014 (2014)

مصطلحات موضوعية: Gynecology and obstetrics, RG1-991

الوصف: Background. Large loop excision of the transformation zone (LLETZ) is routinely performed for the management of high grade intracervical neoplasia (CIN). Several uncommon complications have been described, including postoperative peritonitis, pseudoaneurysm of uterine artery, and bowel fistula. We report a unique case of postoperative vaginal evisceration and the subsequent management. Case. A 73-years-old woman underwent LLETZ for high grade CIN. On postoperative day 3, she was admitted for small bowel evisceration through the vagina. Surgical management was based on combined laparoscopic and transvaginal approach and consisted in bowel inspection and reinstatement, peritoneal washing, and dehiscence repair. Conclusions. Vaginal evisceration is a rare but potentially serious complication of pelvic surgery. This case report is to make clinicians aware of such complication following LLETZ and its management.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2090-6684Test; https://doaj.org/toc/2090-6692Test

الوصول الحر: https://doaj.org/article/a258d49468f54fa6a648bead1f1347f2Test

المؤلفون: Mathilde Bergamelli (11416334), Hélène Martin (232730), Mélinda Bénard (769549), Jérôme Ausseil (92331), Jean-Michel Mansuy (11416337), Ilse Hurbain (168655), Maïlys Mouysset (11416340), Marion Groussolles (11416343), Géraldine Cartron (399558), Yann Tanguy le Gac (11416346), Nathalie Moinard (11416349), Elsa Suberbielle (196793), Jacques Izopet (114143), Charlotte Tscherning (11416352), Graça Raposo (205160), Daniel Gonzalez-Dunia (61852), Gisela D’Angelo (11416355), Cécile E. Malnou (11416358)

مصطلحات موضوعية: Cell Biology, Marine Biology, Cell Development, Proliferation and Death, Cell Metabolism, Cell Neurochemistry, Cellular Interactions (incl. Adhesion, Matrix, Cell Wall), early placenta, extracellular vesicles, congenital infection, human cytomegalovirus, placental histoculture

الوصف: Extracellular vesicles (EVs) have increasingly been recognized as key players in a wide variety of physiological and pathological contexts, including during pregnancy. Notably, EVs appear both as possible biomarkers and as mediators involved in the communication of the placenta with the maternal and fetal sides. A better understanding of the physiological and pathological roles of EVs strongly depends on the development of adequate and reliable study models, specifically at the beginning of pregnancy where many adverse pregnancy outcomes have their origin. In this study, we describe the isolation of small EVs from a histoculture model of first trimester placental explants in normal conditions as well as upon infection by human cytomegalovirus. Using bead-based multiplex cytometry and electron microscopy combined with biochemical approaches, we characterized these small EVs and defined their associated markers and ultrastructure. We observed that infection led to changes in the expression level of several surface markers, without affecting the secretion and integrity of small EVs. Our findings lay the foundation for studying the functional role of EVs during early pregnancy, along with the identification of new predictive biomarkers for the severity and outcome of this congenital infection, which are still sorely lacking.

العلاقة: https://figshare.com/articles/presentation/Presentation_1_Human_Cytomegalovirus_Infection_Changes_the_Pattern_of_Surface_Markers_of_Small_Extracellular_Vesicles_Isolated_From_First_Trimester_Placental_Long-Term_Histocultures_pdf/16600307Test

الإتاحة: https://doi.org/10.3389/fcell.2021.689122.s001Test

المؤلفون: Maha Ayyoub, Alejandra Martinez, Philippe Rochaix, Jean-Pierre Delord, Sébastien Vergez, Jean-Jacques Fournié, Eliane Mery, Aurore Siegfried-Vergnon, Claire Illac, Benjamin Vairel, Jérôme Sarini, Agnès Dupret-Bories, Yann Tanguy Le Gac, Stéphanie Motton, Laurence Gladieff, Gwénaël Ferron, Frédéric Pont, Carine Valle, Virginie Féliu, Lucile Mir-Mesnier, Françoise Lauzéral-Vizcaino, Diana Heaugwane, Marie Tosolini, Carlos Gomez-Roca, Carlos Martinez Gomez, Camille Franchet, Victor Sarradin, Christel Devaud, Marie Michelas, Clara-Maria Scarlata, Camille-Charlotte Balança

الوصف: Although understanding of T-cell exhaustion is widely based on mouse models, its analysis in patients with cancer could provide clues indicating tumor sensitivity to immune checkpoint blockade (ICB). Data suggest a role for costimulatory pathways, particularly CD28, in exhausted T-cell responsiveness to PD-1/PD-L1 blockade. Here, we used single-cell transcriptomic, phenotypic, and functional approaches to dissect the relation between CD8+ T-cell exhaustion, CD28 costimulation, and tumor specificity in head and neck, cervical, and ovarian cancers. We found that memory tumor–specific CD8+ T cells, but not bystander cells, sequentially express immune checkpoints once they infiltrate tumors, leading, in situ, to a functionally exhausted population. Exhausted T cells were nonetheless endowed with effector and tumor residency potential but exhibited loss of the costimulatory receptor CD28 in comparison with their circulating memory counterparts. Accordingly, PD-1 inhibition improved proliferation of circulating tumor–specific CD8+ T cells and reversed functional exhaustion of specific T cells at tumor sites. In agreement with their tumor specificity, high infiltration of tumors by exhausted cells was predictive of response to therapy and survival in ICB-treated patients with head and neck cancer. Our results showed that PD-1 blockade–mediated proliferation/reinvigoration of circulating memory T cells and local reversion of exhaustion occur concurrently to control tumors.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15088e85b65bc7abc71df678f13c4143Test
https://doi.org/10.1158/2326-6066.c.6550446.v1Test

المؤلفون: Maha Ayyoub, Alejandra Martinez, Philippe Rochaix, Jean-Pierre Delord, Sébastien Vergez, Jean-Jacques Fournié, Eliane Mery, Aurore Siegfried-Vergnon, Claire Illac, Benjamin Vairel, Jérôme Sarini, Agnès Dupret-Bories, Yann Tanguy Le Gac, Stéphanie Motton, Laurence Gladieff, Gwénaël Ferron, Frédéric Pont, Carine Valle, Virginie Féliu, Lucile Mir-Mesnier, Françoise Lauzéral-Vizcaino, Diana Heaugwane, Marie Tosolini, Carlos Gomez-Roca, Carlos Martinez Gomez, Camille Franchet, Victor Sarradin, Christel Devaud, Marie Michelas, Clara-Maria Scarlata, Camille-Charlotte Balança

الوصف: Supplementary Figures S1-S10 and Tables S1-S3

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8aa04b91de56803092bd709f5564e145Test
https://doi.org/10.1158/2326-6066.22543884Test

المؤلفون: Manon Daix, Martina Aida Angeles, Stéphanie Motton, Yann Tanguy Le Gac, Gwenael Ferron, Alejandra Martinez

المصدر: International journal of gynecological cancer : official journal of the International Gynecological Cancer Society. 32(11)

مصطلحات موضوعية: Ovarian Neoplasms, Oncology, Obstetrics and Gynecology, Humans, Female, Laparoscopy, Cytoreduction Surgical Procedures, Peritoneal Neoplasms

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc52b0b2b152929c36e992042871a120Test
https://pubmed.ncbi.nlm.nih.gov/36343975Test

المؤلفون: Fabien Vidal, Geneviève Plu-Bureau, Jean-Michel Pouilles, F. Tremollieres, Claire Escanes, Anna Gosset, Yann Tanguy le Gac

المصدر: Menopause. 28:300-306

مصطلحات موضوعية: musculoskeletal diseases, medicine.medical_specialty, Ovariectomy, Endometriosis, Urology, 030209 endocrinology & metabolism, 03 medical and health sciences, Surgical Menopause, Absorptiometry, Photon, 0302 clinical medicine, Bone Density, medicine, Humans, Femoral neck, Bone mineral, Lumbar Vertebrae, 030219 obstetrics & reproductive medicine, Femur Neck, business.industry, Obstetrics and Gynecology, Odds ratio, Middle Aged, medicine.disease, Menopause, medicine.anatomical_structure, Case-Control Studies, Menarche, Female, business, Body mass index

الوصف: Objective To study bone mineral density (BMD) in women with and without pelvic deep infiltrating endometriosis (DIE) who underwent early bilateral oophorectomy (BO). Methods A case-control study was performed in 83 women who underwent early BO before the age of 45 years, 31 for DIE and 52 for another clinical condition. All the women answered a standardized computer-assisted questionnaire to record their clinical and historical data and were medically examined. Lumbar spine and femoral neck BMDs were measured by dual-energy X-ray absorptiometry after early BO. Simultaneously, serum calcium, intact parathyroid, 25-hydroxyvitamin D, and cross-linked C-telopeptide were also measured. Unadjusted and adjusted odds ratios (with 95% confidence intervals [CI]) for endometriosis were calculated using logistic regression. Results The mean lumbar spine and femoral neck BMDs were significantly higher in women who underwent early BO for DIE than in those who underwent early BO for another clinical condition. After adjusting for age at BMD measurement, years since menopause, age at menarche and body mass index, odds ratio for endometriosis associated with a 1-SD increase in lumbar spine and femoral neck BMD was 2.59 (95% CI: 1.45-4.62) and 2.16 (95% CI: 1.23-3.81), respectively. Conclusion Higher lumbar spine and femoral neck BMDs are associated with an increase in the likelihood of pelvic DIE in women who underwent early BO. This might be expected to the extent that endometriosis is itself associated with enhanced estrogen status, although further studies are needed to confirm such a hypothesis. These findings suggest that BMD measurement could contribute to the hormonal management of surgical menopause in women with DIE.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7b8047c52ddc35b12797b4900aa8103Test
https://doi.org/10.1097/gme.0000000000001696Test