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1دورية أكاديمية
المؤلفون: HU Chenyang, LU Shaoyong, YANG Xiuyan
المصدر: Shanghai Jiaotong Daxue xuebao. Yixue ban, Vol 44, Iss 5, Pp 567-575 (2024)
مصطلحات موضوعية: sumo-activating enzyme subunit 1 (sae1), sumo-activating enzyme subunit 2 (sae2), peptide inhibitor, drug screening, isothermal calorimetry (itc), fluorescence polarization (fp), surface plasmon resonance (spr), enzyme activity-based fluorescence assay, Medicine
الوصف: Objective·To establish various in vitro screening systems for the discovery of peptide inhibitors targeting the interaction between small ubiquitin-like modifier (SUMO)-activating enzyme subunit 1 (SAE1) and subunit 2 (SAE2), as well as to evaluate their advantages, disadvantages, and applicability to this research.Methods·The DNA fragments encoding human SAE1 and SAE2 were cloned into vector pET-28a, respectively, to generate protein SAE1 and SAE2. Purified proteins were used to establish screening assays, including isothermal calorimetry (ITC), fluorescence polarization (FP), surface plasmon resonance (SPR) and a fluorescence assay based on the SAE enzyme activity. The inhibitory activity of peptide candidates in different screening systems was examined, and their performance in terms of sensitivity, robustness, throughput and cost was evaluated.Results·The dissociation constant (Kd) of in vitro SAE1 and SAE2 interaction was determined to be 0.96 μmol/L by ITC, and PEPT7 was identified as the most potent peptide. However, the tracer of FP, which was derived from PEPT7, was not up to snuff due to its low affinity with SAE2. In the SPR assay, the Kd value (=1.13 μmol/L) of SAE1 and SAE2 interaction was in line with the results from ITC. The SAE enzyme activity-based screening assay revealed that HP1B, the most effective peptide, inhibited SAE with an half-maximal inhibitory concentration (IC50) of 15.72 μmol/L. The affinity of HP1B for SAE1 was determined to be 34.4 μmol/L by SPR.Conclusion·Several common screening systems for protein-protein interation (PPI) inhibitors are established and compared. Among them, ITC does not allow for high-throughput screening and it is difficult to accurately evaluate the low-affinity polypeptides with insignificant binding heat. The feasibility of FP relies heavily on the strong affinity between a tracer peptide and the protein target, making it unsuitable for the screening and optimization of low-affinity peptides. SPR is highly sensitive but the cost is high. The SAE enzyme activity-based assay stands out because it is a combination of high sensitivity, robustness, throughput and acceptable cost.
وصف الملف: electronic resource
العلاقة: https://xuebao.shsmu.edu.cn/article/2024/1674-8115/1674-8115-2024-44-5-567.shtmlTest; https://doaj.org/toc/1674-8115Test
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2دورية أكاديمية
المؤلفون: Fu, Zhang, Yang, Xiuyan, Jiang, Youheng, Mao, Xinliang, Liu, Hualin, Yang, Yanming, Chen, Jia, Chen, Zhumei, Li, Huiliang, Zhang, Xue-Song, Mao, Xinjun, Li, Ningning, Wang, Dilong, Jiang, Jian
المصدر: Frontiers in Microbiology , 15 , Article 1331130. (2024)
مصطلحات موضوعية: 16S rRNA, 16p11.2, ASD, gut microbiota, histamine, metabolomic, neurotransmitter
الوصف: The gut-brain axis is evident in modulating neuropsychiatric diseases including autism spectrum disorder (ASD). Chromosomal 16p11.2 microduplication 16p11.2dp/+ is among the most prevalent genetic copy number variations (CNV) linked with ASD. However, the implications of gut microbiota status underlying the development of ASD-like impairments induced by 16p11.2dp/+ remains unclear. To address this, we initially investigated a mouse model of 16p11.2dp/+, which exhibits social novelty deficit and repetitive behavior characteristic of ASD. Subsequently, we conducted a comparative analysis of the gut microbial community and metabolomic profiles between 16p11.2dp/+ and their wild-type counterparts using 16S rRNA sequencing and liquid chromatography-mass spectrometry (LC/MS). Our microbiota analysis revealed structural dysbiosis in 16p11.2dp/+ mice, characterized by reduced biodiversity and alterations in species abundance, as indicated by α/β-diversity analysis. Specifically, we observed reduced relative abundances of Faecalibaculum and Romboutsia, accompanied by an increase in Turicibacter and Prevotellaceae UCG_001 in 16p11.2dp/+ group. Metabolomic analysis identified 19 significantly altered metabolites and unveiled enriched amino acid metabolism pathways. Notably, a disruption in the predominantly histamine-centered neurotransmitter network was observed in 16p11.2dp/+ mice. Collectively, our findings delineate potential alterations and correlations among the gut microbiota and microbial neurotransmitters in 16p11.2dp/+ mice, providing new insights into the pathogenesis of and treatment for 16p11.2 CNV-associated ASD.
وصف الملف: application/pdf
العلاقة: https://discovery.ucl.ac.uk/id/eprint/10190633/1/fmicb-15-1331130.pdfTest; https://discovery.ucl.ac.uk/id/eprint/10190633Test/
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3دورية أكاديمية
المؤلفون: Jiang, Jian, Wang, Dilong, Jiang, Youheng, Yang, Xiuyan, Sun, Runfeng, Chang, Jinlong, Zhu, Wenhui, Yao, Peijia, Song, Kun, Chang, Shuwen, Wang, Hong, Zhou, Lei, Zhang, Xue-Song, Li, Huiliang, Li, Ningning
المصدر: Microbiome , 12 , Article 66. (2024)
مصطلحات موضوعية: Autism, Social deficits, Gut microbiota metabolite, Indole-3-propionic acid, Mapk3, GABA
الوصف: Background: Microdeletion of the human chromosomal region 16p11.2 (16p11.2+/−) is a prevalent genetic factor associated with autism spectrum disorder (ASD) and other neurodevelopmental disorders. However its pathogenic mechanism remains unclear, and efective treatments for 16p11.2+/− syndrome are lacking. Emerging evidence suggests that the gut microbiota and its metabolites are inextricably linked to host behavior through the gut-brain axis and are therefore implicated in ASD development. Despite this, the functional roles of microbial metabo‑ lites in the context of 16p11.2+/− are yet to be elucidated. This study aims to investigate the therapeutic potential of indole-3-propionic acid (IPA), a gut microbiota metabolite, in addressing behavioral and neural defcits associated with 16p11.2+/−, as well as the underlying molecular mechanisms. // Results: Mice with the 16p11.2+/− showed dysbiosis of the gut microbiota and a signifcant decrease in IPA levels in feces and blood circulation. Further, these mice exhibited signifcant social and cognitive memory impairments, along with hyperactivation of hippocampal dentate gyrus neurons and reduced inhibitory synaptic transmission in this region. However, oral administration of IPA efectively mitigated the histological and electrophysiological alterations, thereby ameliorating the social and cognitive defcits of the mice. Remarkably, IPA treatment signifcantly increased the phosphorylation level of ERK1, a protein encoded by the Mapk3 gene in the 16p11.2 region, with‑ out afecting the transcription and translation of the Mapk3 gene. // Conclusions: Our study reveals that 16p11.2+/− leads to a decline in gut metabolite IPA levels; however, IPA supple‑ mentation notably reverses the behavioral and neural phenotypes of 16p11.2+/− mice. These fndings provide new insights into the critical role of gut microbial metabolites in ASD pathogenesis and present a promising treatment strategy for social and cognitive memory defcit disorders, such as 16p11.2 microdeletion syndrome.
وصف الملف: text
العلاقة: https://discovery.ucl.ac.uk/id/eprint/10190060/1/s40168-024-01755-7%20%282%29.pdfTest; https://discovery.ucl.ac.uk/id/eprint/10190060Test/
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4دورية أكاديمية
المؤلفون: Wang, Xuefei, Du, Zeqian, Guo, Yuegui, Zhong, Jie, Song, Kun, Wang, Junyuan, Yu, Jianqiang, Yang, Xiuyan, Liu, Chen-Ying, Shi, Ting, Zhang, Jian
المصدر: Acta Pharmaceutica Sinica B ; ISSN 2211-3835
مصطلحات موضوعية: General Pharmacology, Toxicology and Pharmaceutics
الإتاحة: https://doi.org/10.1016/j.apsb.2024.03.020Test
https://api.elsevier.com/content/article/PII:S2211383524001011?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2211383524001011?httpAccept=text/plainTest -
5دورية أكاديمية
المؤلفون: Xu, Xinyuan, Zhang, Qian, Wang, Xufeng, Jin, Jing, Wu, Chengwei, Feng, Li, Yang, Xiuyan, Zhao, Mingzhu, Chen, Yingyi, Lu, Shaoyong, Zheng, Zhen, Lan, Xiaobing, Wang, Yi, Zheng, Yan, Lu, Xuefeng, Zhang, Qiufen, Zhang, Jian
المساهمون: National Natural Science Foundation of China, China Postdoctoral Science Foundation, National Key Research and Development Program of China
المصدر: Acta Pharmaceutica Sinica B ; volume 14, issue 3, page 1302-1316 ; ISSN 2211-3835
مصطلحات موضوعية: General Pharmacology, Toxicology and Pharmaceutics
الإتاحة: https://doi.org/10.1016/j.apsb.2023.11.014Test
https://api.elsevier.com/content/article/PII:S2211383523004379?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2211383523004379?httpAccept=text/plainTest -
6تقرير
المؤلفون: Zhang, Hao, Yang, Xiuyan, Ma, Jianwei
المصدر: Geophysics 85(4): WA115-WA136, 2020
مصطلحات موضوعية: Physics - Geophysics, Computer Science - Machine Learning, Electrical Engineering and Systems Science - Signal Processing
الوصف: We propose a convolutional neural network (CNN) denoising based method for seismic data interpolation. It provides a simple and efficient way to break though the lack problem of geophysical training labels that are often required by deep learning methods. The new method consists of two steps: (1) Train a set of CNN denoisers from natural image clean-noisy pairs to learn denoising; (2) Integrate the trained CNN denoisers into project onto convex set (POCS) framework to perform seismic data interpolation. The method alleviates the demanding of seismic big data with similar features as applications of end-to-end deep learning on seismic data interpolation. Additionally, the proposed method is flexible for many cases of traces missing because missing cases are not involved in the training step, and thus it is of plug-and-play nature. These indicate the high generalizability of our approach and the reduction of the need of the problem-specific training. Primary results on synthetic and field data show promising interpolation performances of the presented CNN-POCS method in terms of signal-to-noise ratio, de-aliasing and weak-feature reconstruction, in comparison with traditional $f$-$x$ prediction filtering and curvelet transform based POCS methods.
Comment: 26 pages, 7 figures, 2 tablesالوصول الحر: http://arxiv.org/abs/1902.10379Test
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7دورية أكاديمية
المؤلفون: Yang Xiuyan, Luo Yingchun
المصدر: Zeitschrift für Kristallographie - New Crystal Structures, Vol 237, Iss 5, Pp 833-835 (2022)
مصطلحات موضوعية: 2180327, Physics, QC1-999, Crystallography, QD901-999
الوصف: C24H16Br2N2O4Zn, monoclinic, I2/a (no. 15), a = 7.4191(9) Å, b = 24.458(3) Å, c = 25.810(3) Å, β = 92.426(3)°, V = 4679.2(10) Å3, Z = 8, R gt(F) = 0.0514, wR ref(F 2) = 0.1710, T = 296(2) K.
وصف الملف: electronic resource
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8دورية أكاديمية
المؤلفون: Zhu, Wenhui, Li, Weifen, Jiang, Jian, Wang, Dilong, Mao, Xinliang, Zhang, Jin, Zhang, Xunzhi, Chang, Jinlong, Yao, Peijia, Yang, Xiuyan, Da Costa, Clive, Zhang, Ying, Yu, Jiezhong, Li, Huiliang, Li, Shupeng, Chi, Xinjin, Li, Ningning
المصدر: Frontiers in Molecular Neuroscience , 16 , Article 1071327. (2023)
مصطلحات موضوعية: Chronic restraint stress, depression, hippocampus, p38/JNK signaling pathway, salmon calcitonin
الوصف: Depression is a common recurrent psychiatric disorder with a high lifetime prevalence and suicide rate. At present, although several traditional clinical drugs such as fluoxetine and ketamine, are widely used, medications with a high efficiency and reduced side effects are of urgent need. Our group has recently reported that a single administration of salmon calcitonin (sCT) could ameliorate a depressive-like phenotype via the amylin signaling pathway in a mouse model established by chronic restraint stress (CRS). However, the molecular mechanism underlying the antidepressant effect needs to be addressed. In this study, we investigated the antidepressant potential of sCT applied chronically and its underlying mechanism. In addition, using transcriptomics, we found the MAPK signaling pathway was upregulated in the hippocampus of CRS-treated mice. Further phosphorylation levels of ERK/p38/JNK kinases were also enhanced, and sCT treatment was able only to downregulate the phosphorylation level of p38/JNK, with phosphorylated ERK level unaffected. Finally, we found that the antidepressant effect of sCT was blocked by p38 agonists rather than JNK agonists. These results provide a mechanistic explanation of the antidepressant effect of sCT, suggesting its potential for treating the depressive disorder in the clinic.
وصف الملف: text
العلاقة: https://discovery.ucl.ac.uk/id/eprint/10167419/1/fnmol-16-1071327.pdfTest; https://discovery.ucl.ac.uk/id/eprint/10167419Test/
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9دورية أكاديمية
المؤلفون: Moog, Max W., Yang, Xiuyan, Bendtsen, Amalie K., Dong, Lin, Crocoll, Christoph, Imamura, Tomohiro, Mori, Masashi, Cushman, John C., Kant, Merijn R., Palmgren, Michael
المصدر: Moog , M W , Yang , X , Bendtsen , A K , Dong , L , Crocoll , C , Imamura , T , Mori , M , Cushman , J C , Kant , M R & Palmgren , M 2023 , ' Epidermal bladder cells as a herbivore defense mechanism ' , Current Biology , vol. 33 , no. 21 , pp. 4662-4673.e6 . https://doi.org/10.1016/j.cub.2023.09.063Test
مصطلحات موضوعية: abiotic stress, biotic stress, Chenopodium quinoa, drought tolerance, epidermal bladder cells, herbivory, Mesembryanthemum crystallinum, phytopathogen, salt tolerance
الوصف: The aerial surfaces of quinoa (Chenopodium quinoa) and common ice plant (Mesembryanthemum crystallinum) are covered with a layer of epidermal bladder cells (EBCs), which are modified non-glandular trichomes previously considered to be key to the extreme salt and drought tolerance of these plants. Here, however, we find that EBCs of these plants play only minor roles, if any, in abiotic stress tolerance and in fact are detrimental under conditions of water deficit. We report that EBCs instead function as deterrents to a broad range of generalist arthropod herbivores, through their combined function of forming both a chemical and a physical barrier, and they also serve a protective function against a phytopathogen. Our study overturns current models that link EBCs to salt and drought tolerance and assigns new functions to these structures that might provide novel possibilities for protecting crops from arthropod pests.
وصف الملف: application/pdf
الإتاحة: https://doi.org/10.1016/j.cub.2023.09.063Test
https://curis.ku.dk/portal/da/publications/epidermal-bladder-cells-as-a-herbivore-defense-mechanismTest(83e7d2b5-2455-4c78-be24-09f7b597fad8).html
https://curis.ku.dk/ws/files/375548991/1_s2.0_S096098222301312X_main.pdfTest -
10دورية أكاديمية
المؤلفون: Wu, YanXiang, Yang, XiuYan, Hu, YuanYuan, Hu, XueHong, Zhang, YueLin, An, Tian, Lv, BoHan, Tao, SiYu, Liu, Qing, Jiang, GuangJian
المصدر: Food Science & Nutrition ; volume 11, issue 9, page 5137-5156 ; ISSN 2048-7177 2048-7177
الوصف: This study investigated the effects of supplementation Moringa oleifera leaf (MOL) on relieving oxidative stress, anti‐inflammation, changed the relative abundance of multiple intestinal flora and blood biochemical indices during letrozole‐induced polycystic ovary syndrome (PCOS). Previous studies have shown that MOL has anti‐inflammatory, anti‐oxidation, insulin‐sensitizing effects. However, whether MOL has beneficial effects on PCOS remains to be elucidated. In the current study, 10‐week‐old female Sprague–Dawley rats received letrozole to induce PCOS‐like rats, and subsequently were treated with a MOL diet. Then, the body weight and estrus cycles were measured regularly in this period. Finally, the ovarian morphology, blood biochemical indices, anti‐oxidative, intestinal flora, and anti‐inflammation were observed at the end of the experiment. We found that MOL supplementation markedly decreased the body weight, significantly upregulated the expression of Sirt1, FoxO1, PGC‐1α, IGF1, and substantially modulated the sex hormone level and improved insulin resistance, which may be associated with the relieves oxidative stress. Moreover, the supplementation of MOL changed the relative abundance of multiple intestinal flora, the relative abundance of Fusobacterium , Prevotella were decreased, and Blautia and Parabacteroides were increased. These results indicate that MOL is potentially a supplementary medication for the management of PCOS.
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