المؤلفون: Dan-Qing Wang, Yuan-Yuan Wang, Yan-Ling Shi, Bin Zeng, Zi-Jing Lin, Qin Deng, Jia Ming

المصدر: Heliyon, Vol 9, Iss 8, Pp e18697- (2023)

مصطلحات موضوعية: Breast cancer, Circulating tumor cells, Connexin 43, Epithelial-mesenchymal transition, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: Background: Connexin 43 (Cx43) has been closely linked to the occurrence and progression of breast cancer. Distant metastasis of breast cancer is aided by the epithelial-mesenchymal transition of circulating tumor cells (CTCs). However, the impact of Cx43 expression on CTCs and the extent of its role in the disease remain unclear. Methods: We determined CTCs in 156 patients, who had breast cancer with a disease course of two or more years. We also measured the expression of Cx43 in the CTCs. The CTCs were detected in the blood of 139 of these patients. These 139 patients were divided into two groups: the Cx43 group and the non-Cx43 group based on their Cx43 expression. Results: Overall, Cx43 expression was found in 83 of the 139 patients (59.7%, 83/139 cases). The two groups significantly differed in terms of the number of mixed biphenotypic type CTCs and the total number of CTCs (P

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844023059054Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/a7aa1fd795dc4c0fa60804c5fcdf0120Test

المؤلفون: Yan-Ling Shi, Yue-Ping Zhang, Huan Luo, Fan Xu, Jian-Mei Gao, Jing-Shan Shi, Qi-Hai Gong

المصدر: Frontiers in Pharmacology, Vol 13 (2022)

مصطلحات موضوعية: trilobatin, T2DM, insulin resistance, lipid metabolism, Nrf2/ARE signaling pathway, insulin signaling transduction pathway, Therapeutics. Pharmacology, RM1-950

الوصف: Oxidative stress and aberrant insulin signaling transduction play vital roles in type 2 diabetes mellitus (T2DM). Our previous research has demonstrated that trilobatin (TLB), derived from the leaves of Lithocarpus Polystachyus (Wall.), exhibits a potent antioxidative profile. In the current study, we investigated the anti-T2DM effect of TLB on KK-Ay diabetic mice and further explored the potential mechanisms. Our results showed that TLB significantly reduced the high fasting blood glucose level and insulin resistance and promoted the tolerances to exogenous glucose and insulin in KK-Ay mice. Moreover, TLB reduced the content of reactive oxygen species; enhanced antioxidant enzymes activities, including serum catalase, glutathione peroxidase, and superoxide dismutase; and regulated the abnormal parameters of lipid metabolism, including triglyceride, high-density lipoprotein-cholesterol, low-density lipoprotein-cholesterol, and free fatty acid, as evidenced by enzyme-linked immunosorbent assay. Additionally, TLB markedly ameliorated the pancreatic islet morphology near normal and increased the insulin expression of the islet. Whereafter, TLB promoted Nrf2 that was translocated from cytoplasm to nucleus. Moreover, it increased the protein expressions of HO-1, NQO-1, and GLUT-2, and phosphorylation levels of Akt and GSK-3βSer 9 and decreased the protein expressions of keap1 and phosphorylation levels of IRS-1Ser 307 and GSK-3βTyr 216. Taken together, our findings reveal that TLB exhibits an anti-T2DM effect in KK-Ay mice by activating the Nrf2/ARE signaling pathway and regulating insulin signaling transduction pathway, and TLB is promising to be developed into a novel candidate for the treatment of T2DM in clinic due to its favorable druggability.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fphar.2022.828473/fullTest; https://doaj.org/toc/1663-9812Test

الوصول الحر: https://doaj.org/article/5f83f567d89f415fa00feb73c94039d6Test

المؤلفون: Na Li, Xi Li, Yan-Ling Shi, Jian-Mei Gao, Yu-Qi He, Fei Li, Jing-Shan Shi, Qi-Hai Gong

المصدر: Frontiers in Pharmacology, Vol 12 (2021)

مصطلحات موضوعية: trilobatin, anti-aging, Caenorhabditis elegans, SKN1, SIRT3, DAF16, Therapeutics. Pharmacology, RM1-950

الوصف: Trilobatin (TLB) is an effective component from Lithocarpus polystachyrus Rehd. Our previous study revealed that TLB protected against oxidative injury in neuronal cells by AMPK/Nrf2/SIRT3 signaling pathway. However, whether TLB can delay aging remains still a mystery. Therefore, the present study was designed to investigate the possible longevity-enhancing effect of TLB, and further to explore its underlying mechanism in Caenorhabditis elegans (C. elegans). The results showed that TLB exerted beneficial effects on C. elegans, as evidenced by survival rate, body movement assay and pharynx-pumping assay. Furthermore, TLB not only significantly decreased ROS and MDA levels, but also increased anti-oxidant enzyme activities including CAT and SOD, as well as its subtypes SOD2 andSOD3, but not affect SOD1 activity, as evidenced by heat and oxidative stress resistance assays. Whereas, the anti-oxidative effects of TLB were almost abolished in SKN1, Sir2.3, and DAF16 mutant C. elegans. Moreover, TLB augmented the fluorescence intensity of DAF16: GFP, SKN1:GFP, GST4:GFP mutants, indicating that TLB increased the contents of SKN1, SIRT3 and DAF16 due to fluorescence intensity of these mutants, which were indicative of these proteins. In addition, TLB markedly increased the protein expressions of SKN1, SIRT3 and DAF16 as evidenced by ELISA assay. However, its longevity-enhancing effect were abolished in DAF16, Sir2.3, SKN1, SOD2, SOD3, and GST4 mutant C. elegans than those of non-TLB treated controls. In conclusion, TLB effectively prolongs lifespan of C. elegans, through regulating redox homeostasis, which is, at least partially, mediated by SKN1/SIRT3/DAF16 signaling pathway.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fphar.2021.655045/fullTest; https://doaj.org/toc/1663-9812Test

الوصول الحر: https://doaj.org/article/f6038d95b94b44b4b032cab88d80ea04Test

المؤلفون: Zhou Xu, Yu-Tuan Wu, Xin Li, He Wu, Hao-Ran Chen, Yan-Ling Shi, Bilal Arshad, Hong-Yuan Li, Kai-Nan Wu, Ling-Quan Kong

المصدر: Chinese Medical Journal, Vol 130, Iss 16, Pp 1995-1996 (2017)

مصطلحات موضوعية: Autotransplantation, Dialysis, Parathyroidectomy, Secondary Hyperparathyroidism, Thyrotoxicosis, Medicine

وصف الملف: electronic resource

العلاقة: http://www.cmj.org/article.asp?issn=0366-6999;year=2017;volume=130;issue=16;spage=1995;epage=1996;aulast=XuTest; https://doaj.org/toc/0366-6999Test

الوصول الحر: https://doaj.org/article/d97cfbd61f65424785eea1f9571c67afTest

المؤلفون: Yan-Ling Shi, 施燕玲

مرشدي الرسالة: Chang-Hsien Yang, 楊長賢

الوصف: 89
The formation of unisexual flowers in Zucchini squash, a monoecious plant, results from the suppression of either male or female organs during flower development. Since MADS box genes play the central role for flower development, it is intriguing to investigate of the relationship between MADS box genes and the sex-determined mechanism. For this, two cDNAs showing high homology to floral organ identity MADS box genes were isolated and characterized from Z. squash. ZuAG (Zucchini AG) encodes a 230-amino acid protein that showed 92% identity and 96% similarity to CUM1, an AGAMOUS (AG) homologue in cucumber (Cucumis sativus). ZuAGL2 (Zucchini AGL2) encodes a 246-amino acid protein that showed 90% identity and 91% similarity to CAGL2, an AGL2 homologue in cucumber. Result of Southern blot analysis indicated that both ZuAG and ZuAGL2 DNA sequences are present in Z. squash genome in a single copy manner. ZuAG and ZuAGL2 mRNA were detected in floral buds of different stages but absent in vegetative leaf, suggesting that the expression of these two genes is floral specific. The expression of ZuAG was restricted in the stamen of a male flower and the carpel of a female flower; whereas ZuAGL2 was expressed in all four floral whorls. These data indicate that ZuAG and ZuAGL2 may act independently from the formation of unisexual flowers in Z. squash. Ectopic expression of ZuAG in transgenic Arabidopsis plants showed novel phenotypes with early flowering and curly leaves. In flowers, homeotic conversion of sepals to carpelloid and petal to stamen-like structures were observed in 35S::ZuAG transgenic plants. This supported that ZuAG is an AG homologue in Z. squash. Further functional analysis for ZuAG was also performed by cloning of full length and truncated cDNA for ZuAG into Zucchini yellow mosaic virus (ZYMV) infectious clone and introduced to zucchini plants. The female flowers were produced earlier in inoculated plants than in non inoculated plants. However, no obvious homeotic conversion for floral organs was observed in inoculated plants.

وصف الملف: 112

الإتاحة: http://ndltd.ncl.edu.tw/handle/81243449555679816281Test

المؤلفون: Xue-Ke Wang, Ya-Bin Sun, Yun Liu, Xiao-Jin Li, Yan-Ling Shi

المصدر: 2022 IEEE 16th International Conference on Solid-State & Integrated Circuit Technology (ICSICT).

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::94b8c88ed3843d4f575c609254098d53Test
https://doi.org/10.1109/icsict55466.2022.9963313Test

المؤلفون: Xue-Ke Wang, Ya-Bin Sun, Zi-Yu Liu, Yun Liu, Xiao-Jin Li, Yan-Ling Shi

المصدر: Chinese Physics B.

مصطلحات موضوعية: General Physics and Astronomy

الوصف: In this paper, the single event transient (SET) effect in nanotube tunneling field-effect transistor with bias-induced electron-hole bilayer (EHBNT-TFET) is investigated by 3-D TCAD simulation for the first time. The effects of linear energy transfer (LET), characteristic radius, strike angle, electrode bias and hit location on SET response are evaluated in detail. The simulation results show that the peak value of transient drain current is up to 0.08 mA for heavy ion irradiation with characteristic radius of 50 nm and LET of 10 MeV·cm2/mg, which is much higher than ON-state current of EHBNT-TFET. The SET response of EHBNT-TFET presents an obvious dependence on LET, strike angle, drain bias and hit location. As LET increases from 2 MeV·cm2/mg to 10 MeV·cm2/mg, the peak drain current increases monotonically from 0.015 mA to 0.08 mA. The strike angle has an greater impact on peak drain current especially for the smaller characteristic radius. The peak drain current and collected charge increases 0.014 mA and 0.06 fC respectively as drain bias ranges from 0.1 V to 0.9 V. Whether from horizontal or vertical direction, the most sensitive hit location is related to w t . The underlying physical mechanism is explored and discussed.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::ef2fb0a9c78002a4bab0021f85c2def5Test
https://doi.org/10.1088/1674-1056/ac9180Test

المؤلفون: Fei-Shen Lin, Xiao-Li Zhai, Wei-Wei Gao, Xi-Wei Lu, Yi-Xiang J. Wang, Jian-Yun Wang, Hui-Shan Yu, Xin-Hua Zhou, Wen-Shu Chai, Chun-Mei Hu, Yi Zeng, Yan-Ling Shi

المصدر: Quant Imaging Med Surg

مصطلحات موضوعية: Miliary tuberculosis, medicine.medical_specialty, Tuberculosis, integumentary system, medicine.diagnostic_test, Pneumatocele, business.industry, Incidence (epidemiology), Computed tomography, Review Article, macromolecular substances, medicine.disease, environment and public health, Ground-glass opacity, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, 030228 respiratory system, medicine, Radiology, Nuclear Medicine and imaging, Radiology, medicine.symptom, Differential diagnosis, business, Halo sign

الوصف: Tuberculosis is a serious public health challenge facing mankind and one of the top ten causes of death. Diagnostic imaging plays an important role, particularly for the diagnosis and treatment planning of tuberculosis patients with negative microbiology results. This article illustrates a number of atypical computed tomography (CT) appearances of pulmonary tuberculosis (PTB), including (I) clustered micronodules (CMNs) sign; (II) reversed halo sign (RHS); (III) tuberculous pneumatocele; (IV) hematogenously disseminated PTB with predominantly diffuse ground glass opacity manifestation; (V) hematogenously disseminated PTB with randomly distributed non-miliary nodules; (VI) PTB changes occur on the background of emphysema or honeycomb changes of interstitial pneumonia; and (VII) PTB manifesting as organizing pneumonia. While the overall incidence of PTB is decreasing globally, the incidence of atypical manifestations of tuberculosis is increasing. A good understanding of the atypical CT imaging changes of active PTB shall help the diagnosis and differential diagnosis of PTB in clinical practice.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b7bd02d77e6d18330ddff165d93404fTest
https://doi.org/10.21037/qims-20-1323Test

المؤلفون: Zhou Xu, Jia-shuo Liu, Ze Wang, Hong Li, Shen Tian, Lingquan Kong, Yan-Ling Shi, Hao-Ran Chen, Shu Li, Bao-shan Zou, Kai-nan Wu, Hao Li, Liang Ran

المصدر: Thyroid. 30:1150-1158

مصطلحات موضوعية: Adult, China, medicine.medical_specialty, Adolescent, Cross-sectional study, Endocrinology, Diabetes and Metabolism, Breast Neoplasms, 030209 endocrinology & metabolism, BI-RADS, Asymptomatic, Body Mass Index, Cohort Studies, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Odds Ratio, Prevalence, medicine, Humans, Thyroid Neoplasms, Aged, Ultrasonography, Aged, 80 and over, Metabolic Syndrome, Anthropometry, business.industry, Obstetrics, Thyroid, Middle Aged, Cross-Sectional Studies, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Multivariate Analysis, Regression Analysis, Female, medicine.symptom, business

الوصف: Introduction: Prior research has shown an association between breast and thyroid cancers, although their relationship is unclear. In China, asymptomatic women undergoing regular health checkups usu...

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::938b3deca4b431ff24543edf1c7afdb8Test
https://doi.org/10.1089/thy.2019.0184Test

المؤلفون: Yan-Ling Shi, Yue-Ping Zhang, Huan Luo, Fan Xu, Jian-Mei Gao, Jing-Shan Shi, Qi-Hai Gong

المصدر: Frontiers in Pharmacology, Vol 13 (2022)

مصطلحات موضوعية: Pharmacology, trilobatin, insulin resistance, lipid metabolism, T2DM, Nrf2/ARE signaling pathway, insulin signaling transduction pathway, Pharmacology (medical), Therapeutics. Pharmacology, RM1-950

الوصف: Oxidative stress and aberrant insulin signaling transduction play vital roles in type 2 diabetes mellitus (T2DM). Our previous research has demonstrated that trilobatin (TLB), derived from the leaves of Lithocarpus Polystachyus (Wall.), exhibits a potent antioxidative profile. In the current study, we investigated the anti-T2DM effect of TLB on KK-Ay diabetic mice and further explored the potential mechanisms. Our results showed that TLB significantly reduced the high fasting blood glucose level and insulin resistance and promoted the tolerances to exogenous glucose and insulin in KK-Ay mice. Moreover, TLB reduced the content of reactive oxygen species; enhanced antioxidant enzymes activities, including serum catalase, glutathione peroxidase, and superoxide dismutase; and regulated the abnormal parameters of lipid metabolism, including triglyceride, high-density lipoprotein-cholesterol, low-density lipoprotein-cholesterol, and free fatty acid, as evidenced by enzyme-linked immunosorbent assay. Additionally, TLB markedly ameliorated the pancreatic islet morphology near normal and increased the insulin expression of the islet. Whereafter, TLB promoted Nrf2 that was translocated from cytoplasm to nucleus. Moreover, it increased the protein expressions of HO-1, NQO-1, and GLUT-2, and phosphorylation levels of Akt and GSK-3βSer 9 and decreased the protein expressions of keap1 and phosphorylation levels of IRS-1Ser 307 and GSK-3βTyr 216. Taken together, our findings reveal that TLB exhibits an anti-T2DM effect in KK-Ay mice by activating the Nrf2/ARE signaling pathway and regulating insulin signaling transduction pathway, and TLB is promising to be developed into a novel candidate for the treatment of T2DM in clinic due to its favorable druggability.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f967c1ffd355ede68e71b37912ce896Test
https://pubmed.ncbi.nlm.nih.gov/35153796Test