المؤلفون: Xingdong Yang, Qingmei Li, Sharon Kwee, Jifei Yang, Qianqian Zhang, Xiaofei Hu

المصدر: PLoS ONE, Vol 19, Iss 3, p e0299709 (2024)

مصطلحات موضوعية: Medicine, Science

الوصف: Marbofloxacin (MBF) was once widely used as a veterinary drug to control diseases in animals. MBF residues in animal food endanger human health. In the present study, an immunochromatographic strip assay (ICSA) utilizing a competitive principle was developed to rapidly detect MBF in beef samples. The 50% inhibitory concentration (IC50) and the limit of detection (LOD) of the ICSAs were 2.5 ng/mL and 0.5 ng/mL, respectively. The cross-reactivity (CR) of the MBF ICSAs to Ofloxacin (OFL), enrofloxacin (ENR), norfloxacin (NOR), and Ciprofloxacin (CIP) were 60.98%, 32.05%, 22.94%, and 23.58%, respectively. The CR for difloxacin (DIF) and sarafloxacin (SAR) was less than 0.1%. The recovery rates of MBF in spiked beef samples ranged from 82.0% to 90.4%. The intra-assay and interassay coefficients of variation (CVs) were below 10%. In addition, when the same authentic beef samples were detected in a side-by-side comparison between the ICSAs and HPLC‒MS, no statistically significant difference was observed. Therefore, the proposed ICSAs can be a useful tool for monitoring MBF residues in beef samples in a qualitative and quantitative manner.

وصف الملف: electronic resource

العلاقة: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0299709&type=printableTest; https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/2bef5d1822e04df7bb7f9578aa111334Test

المؤلفون: Xingdong Yang, Yinbiao Wang, Chunmei Song, Xiaofei Hu, Fangyu Wang, Xianyin Zeng

المصدر: Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract An ultrasensitive indirect competitive enzyme-linked immunosorbent assay (ic ELISA) using monoclonal antibodies (mAbs) was developed for the specific detection of diethylstilbestrol (DES) residues. To establish an ELISA based on mAbs, hapten diethylstilbestrol mono-carboxypropyl-ether (DES-MCPE) was chemically synthetized and then conjugated to bovine serum albumin (BSA) for immunization in mice. This ic ELISA was further optimized for DES determination. The sensitivity of the ic ELISA was found to be 0.49 μg/kg and the limit of detection was 0.075 μg/kg. DES residues in salmon meat and pork were tested with the recovery range from 74.0 to 85.2% and the coefficient of variation (CV) was less than 10%. Parallel analysis of DES samples from salmon meat showed comparable results from the ic ELISA with high-performance liquid chromatography. The ic ELISA provides a useful screening method for the quantitative detection of DES residues in animal-derived food.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/47ee0aa3dc9647c1bfa3f2c3879a2e9eTest

المؤلفون: Xingdong Yang, Yinbiao Wang, Jifei Yang, Zhongke Sun, Cuiwei Chu, Zonghao Yue, Lili Li, Xiaofei Hu

المصدر: Food and Agricultural Immunology, Vol 31, Iss 1, Pp 205-216 (2020)

مصطلحات موضوعية: diclofenac, colloidal gold, lateral flow immunoassay, rapid detection, Agriculture (General), S1-972, Immunologic diseases. Allergy, RC581-607

الوصف: A rapid immunochromatographic lateral flow strip test (ILFST) of competitive format for the detection of diclofenac (DCF) in medicinal wine samples was developed. The half-inhibitory concentration (IC50) values of the ILFST for DCF and the limit of detection (LOD) in DCF-spiked samples were 0.66 and 0.15 ng/mL, respectively. Recoveries of DCF from spiked medicinal wine samples were from 84.4% to 85.9% within an assay (intra-assay) and from 83.7% to 88.4% between assays (inter-assay). The coefficients of variation (CV) for intra-assay and inter-assay were 4.10–7.56% and 4.71–8.94%, respectively. These results indicated that the ILFST was highly sensitive and re-producible. The same authentic medicinal wine samples were tested by both ILFST and high-performance liquid chromatography (HPLC) respectively. The results showed no significant differences between the two methods. Therefore, the ILFST can be widely used as a convenient tool for both qualitative and quantitative detection of DCF residues in medicinal wine samples.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/0954-0105Test; https://doaj.org/toc/1465-3443Test

الوصول الحر: https://doaj.org/article/354311011e43403f8b1950eadc29b426Test

المؤلفون: Viviana Parreno, Muqun Bai, Fangning Liu, Jiqiang Jing, Erika Olney, Guohua Li, Ke Wen, Xingdong Yang, Tammy Bui Castellucc, Jacob F. Kocher, Xu Zhou, Lijuan Yuan

المصدر: Vaccines, Vol 10, Iss 9, p 1529 (2022)

مصطلحات موضوعية: rotavirus vaccine, probiotic adjuvant, Lactobacillus rhamnosus GG, Lanzhou trivalent vaccine, gnotobiotic pig, Medicine

الوصف: This preclinical study in the gnotobiotic (Gn) pig model of human rotavirus (HRV) infection and disease evaluates the effect of probiotic Lactobacillus rhamnosus GG (LGG) as a mucosal adjuvant on the immunogenicity and cross-protective efficacy of the Lanzhou live oral trivalent (G2, G3, G4) vaccine (TLV, aka LLR3). Gn pigs were immunized with three doses of TLV with or without concurrent administration of nine doses of LGG around the time of the first dose of the TLV vaccination, and were challenged orally with the virulent heterotypic Wa G1P[8] HRV. Three doses of TLV were highly immunogenic and conferred partial protection against the heterotypic HRV infection. LGG significantly enhanced the intestinal and systemic immune responses and improved the effectiveness of protection against the heterotypic HRV challenge-induced diarrhea and virus shedding. In conclusion, we demonstrated the immune-stimulating effects of probiotic LGG as a vaccine adjuvant and generated detailed knowledge regarding the cross-reactive and type-specific antibody and effector B and T cell immune responses induced by the TLV. Due to the low cost, ease of distribution and administration, and favorable safety profiles, LGG as an adjuvant has the potential to play a critical role in improving rotavirus vaccine efficacy and making the vaccines more cost-effective.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2076-393X/10/9/1529Test; https://doaj.org/toc/2076-393XTest

الوصول الحر: https://doaj.org/article/19ce3df3d3ad4131bb84e034d7b9a1d0Test

المؤلفون: Dongmei Zhi, Wenyue Wu, Bo Xiao, Shile Qi, Rongtao Jiang, Xingdong Yang, Jian Yang, Wenbiao Xiao, Chaorong Liu, Hongyu Long, Vince D. Calhoun, Lili Long, Jing Sui

المصدر: Frontiers in Neuroscience, Vol 14 (2020)

مصطلحات موضوعية: temporal lobe epilepsy, multimodal fusion, methylation levels of NR4A1, functional connectivity, fractional anisotropy, gray matter volume, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571

الوصف: DNA hypermethylation has been widely observed in temporal lobe epilepsy (TLE), in which NR4A1 knockdown has been reported to be able to alleviate seizure severity in mouse model, while the underlying methylation-imaging pathway modulated by aberrant methylation levels of NR4A1 remains to be clarified in patients with TLE. Here, using multi-site canonical correlation analysis with reference, methylation levels of NR4A1 in blood were used as priori to guide fusion of three MRI features: functional connectivity (FC), fractional anisotropy (FA), and gray matter volume (GMV) for 56 TLE patients and 65 healthy controls. Post-hoc correlations were further evaluated between the identified NR4A1-associated brain components and disease onset. Results suggested that higher NR4A1 methylation levels in TLE were related with impaired temporal-cerebellar and occipital-cerebellar FC strength, lower FA in cingulum (hippocampus), and reduced GMV in putamen, temporal pole, and cerebellum. Moreover, findings were also replicated well in both patient subsets with either right TLE or left TLE only. Particularly, right TLE patients showed poorer cognitive abilities and more severe brain impairment than left TLE patients, especially more reduced GMV in thalamus. In summary, this work revealed a potential imaging-methylation pathway modulated by higher NR4A1 methylation in TLE via data mining, which may impact the above-mentioned multimodal brain circuits and was also associated with earlier disease onset and more cognitive deficits.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/article/10.3389/fnins.2020.00727/fullTest; https://doaj.org/toc/1662-453XTest

الوصول الحر: https://doaj.org/article/d250ce90efbc452582ccc0629040522aTest

المؤلفون: Jacob Kocher, Tammy Bui Castellucci, Ke Wen, Guohua Li, Xingdong Yang, Shaohua Lei, Xi Jiang, Lijuan Yuan

المصدر: Pathogens, Vol 10, Iss 7, p 829 (2021)

مصطلحات موضوعية: simvastatin, human norovirus, P particle vaccine, gnotobiotic pig, T cells, protective efficacy, Medicine

الوصف: Noroviruses (NoVs) are a leading cause of acute gastroenteritis worldwide. P particles are a potential vaccine candidate against NoV. Simvastatin is a cholesterol-reducing drug that is known to increase NoV infectivity. In this study, we examined simvastatin’s effects on P particle-induced protective efficacy and T-cell immunogenicity using the gnotobiotic pig model of human NoV infection and diarrhea. Pigs were intranasally inoculated with three doses (100 µg/dose) of GII.4/VA387-derived P particles together with monophosphoryl lipid A and chitosan adjuvants. Simvastatin-fed pigs received 8 mg/day orally for 11 days prior to challenge. A subset of pigs was orally challenged with 10 ID50 of a NoV GII.4/2006b variant at post-inoculation day (PID) 28 and monitored for 7 days post-challenge. Intestinal and systemic T cell responses were determined pre- and postchallenge. Simvastatin abolished the P particle’s protection and significantly increased diarrhea severity after NoV infection. Simvastatin decreased proliferation of virus-specific and non-specific CD8 T cells in duodenum and virus-specific CD4 and CD8 T cells in spleen and significantly reduced numbers of intestinal mononuclear cells in vaccinated pigs. Furthermore, simvastatin significantly decreased numbers of duodenal CD4+IFN-γ+, CD8+IFN-γ+ and regulatory T cells and total duodenal activated CD4+ and CD8+ T cells in vaccinated pigs pre-challenge at PID 28. Following challenge, simvastatin prevented the IFN-γ+ T cell response in spleen of vaccinated pigs. These results indicate that simvastatin abolished P particle vaccine-induced partial protection through, at least in part, impairing T cell immunity. The findings have specific implications for the development of preventive and therapeutic strategies against NoV gastroenteritis, especially for the elderly population who takes statin-type drugs.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2076-0817/10/7/829Test; https://doaj.org/toc/2076-0817Test

الوصول الحر: https://doaj.org/article/ecfb6c7e645345c8b6fb16b92cffdda2Test

المؤلفون: Zhongke Sun, Zonghao Yue, Xingdong Yang, Xinqi Hao, Maoping Song, Lili Li, Can Chen, Cuiwei Chu, Chengwei Li

المصدر: Frontiers in Microbiology, Vol 10 (2019)

مصطلحات موضوعية: Bifidobacterium longum JCM 1217, promoter, phytase, phytate, phosphorus, Microbiology, QR1-502

الوصف: Genetic engineering of probiotics, like bifidobacteria, may improve their microbial cell factory economy. This work designed a novel shuttle plasmid pBPES, which bears exogenous appA and is stable within Bifidobacterium longum JCM 1217. Cloning of three predicted promoters into pBPES proved that all of them drive appA expression in B. longum JCM 1217. Transformation of plasmids pBPES-tu and pBPES-groEL into B. longum JCM1217 resulted in much more phytase secretion suggests Ptu and PgroEL are strong promoters. Further in vitro and in vivo experiments suggested B. longum JCM 1217/pBPES-tu degrades phytate efficiently. In conclusion, the study screened two stronger promoters and constructed a recombinant live probiotic strain for effectively phytase secretion and phytate degradation in gut. The strategy used in the study provided a novel technique for improving the bioaccessibility of phytate and decreasing phosphorus excretion.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/article/10.3389/fmicb.2019.00796/fullTest; https://doaj.org/toc/1664-302XTest

الوصول الحر: https://doaj.org/article/bbc78094540c4a71b5a061e76102508fTest

المؤلفون: Xingdong Yang, Zhongke Sun, Fengshou Tian, Guochao Jia, Jifei Yang, Xiaofei Hu

المصدر: Royal Society Open Science, Vol 5, Iss 8 (2018)

مصطلحات موضوعية: hexoestrol, immunoassay, colloidal gold, lateral flow immunochromatographic strip test (lfist), rapid test, Science

الوصف: A lateral flow immunochromatographic strip test was developed for rapid and sensitive on-site detection of hexoestrol (HES) residues in fish samples with colloidal gold labelling of the anti-HES monoclonal antibody. The strip is composed of a sample pad, a conjugate reagent pad, an absorbent pad and a test membrane containing a control line and a test line. The sensitivity (half inhibitory concentration, IC50) of the strip in the detection of fish extract samples was confirmed to be 1.86 µg kg−1, and the limit of detection value was 0.62 µg kg−1. For intra-assay and inter-assay reproducibility, recoveries of HES-spiked samples ranged from 86.3% to 92.3% and 85.8% to 93.4%, coefficients of variation were 2.91–4.64% and 4.24–5.17%, respectively. High-performance liquid chromatography was employed to confirm the performance of the strip. The strip test takes less than 10 min, and thus provides a repaid method for on-site detection of HES residues.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2054-5703Test

الوصول الحر: https://doaj.org/article/ab383cc0e6774125964d48436bbdb47dTest

المؤلفون: Elizabeth P. Ryan, Nora Jean Nealon, Lijuan Yuan, Xingdong Yang

المصدر: Frontiers in Microbiology, Vol 8 (2017)

مصطلحات موضوعية: probiotic, prebiotic, synbiotic, human rotavirus, diarrhea, metabolomics, Microbiology, QR1-502

الوصف: Human rotavirus (HRV) is a leading cause of severe childhood diarrhea, and there is limited vaccine efficacy in the developing world. Neonatal gnotobiotic pigs consuming a prophylactic synbiotic combination of probiotics and rice bran (Pro+RB) did not exhibit HRV diarrhea after challenge. Multiple immune, gut barrier protective, and anti-diarrheal mechanisms contributed to the prophylactic efficacy of Pro+RB when compared to probiotics (Pro) alone. In order to understand the molecular signature associated with diarrheal protection by Pro+RB, a global non-targeted metabolomics approach was applied to investigate the large intestinal contents and serum of neonatal gnotobiotic pigs. The ultra-high performance liquid chromatography-tandem mass spectrometry platform revealed significantly different metabolites (293 in LIC and 84 in serum) in the pigs fed Pro+RB compared to Pro, and many of these metabolites were lipids and amino acid/peptides. Lipid metabolites included 2-oleoylglycerol (increased 293.40-fold in LIC of Pro+RB, p = 3.04E-10), which can modulate gastric emptying, andhyodeoxycholate (decreased 0.054-fold in the LIC of Pro+RB, p = 0.0040) that can increase colonic mucus production to improve intestinal barrier function. Amino acid metabolites included cysteine (decreased 0.40-fold in LIC, p = 0.033, and 0.62-fold in serum, p = 0.014 of Pro+RB), which has been found to reduce inflammation, lower oxidative stress and modulate mucosal immunity, and histamine (decreased 0.18-fold in LIC, p = 0.00030, of Pro+RB and 1.57-fold in serum, p = 0.043), which modulates local and systemic inflammatory responses as well as influences the enteric nervous system. Alterations to entire LIC and serum metabolic pathways further contributed to the anti-diarrheal and anti-viral activities of Pro+RB such as sphingolipid, mono/diacylglycerol, fatty acid, secondary bile acid, and polyamine metabolism. Sphingolipid and long chain fatty acid profiles influenced the ability of HRV to both infect and replicate within cells, suggesting that Pro+RB created a protective lipid profile that interferes with HRV activity. Polyamines act on enterocyte calcium-sensing receptors to modulate intracellular calcium levels, and may directly interfere with rotavirus replication. These results support that multiple host and probiotic metabolic networks, notably those involving lipid and amino acid/peptide metabolism, are important mechanisms through which Pro+RB protected against HRV diarrhea in neonatal gnotobiotic pigs.

وصف الملف: electronic resource

العلاقة: http://journal.frontiersin.org/article/10.3389/fmicb.2017.00653/fullTest; https://doaj.org/toc/1664-302XTest

الوصول الحر: https://doaj.org/article/b1a750c3552640a591df2a281ccfbc6dTest

المؤلفون: Xingdong Yang, Guohua Li, Ke Wen, Tammy Bui, Fangning Liu, Jacob Kocher, Bernard S Jortner, Marlice Vonck, Kevin Pelzer, Jie Deng, Runan Zhu, Yuyun Li, Yuan Qian, Lijuan Yuan

المصدر: Emerging Microbes and Infections, Vol 3, Iss 1, Pp 1-12 (2014)

مصطلحات موضوعية: adaptive immune responses, animal model, human enterovirus 71, neonatal gnotobiotic pigs, pathogenesis, vaccine evaluation, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502

الوصف: Vaccine development and pathogenesis studies for human enterovirus 71 are limited by a lack of suitable animal models. Here, we report the development of a novel neonatal gnotobiotic pig model using the non-pig-adapted neurovirulent human enterovirus 71 strain BJ110, which has a C4 genotype. Porcine small intestinal epithelial cells, peripheral blood mononuclear cells and neural cells were infected in vitro. Oral and combined oral–nasal infection of 5-day-old neonatal gnotobiotic pigs with 5×108 fluorescence forming units (FFU) resulted in shedding up to 18 days post-infection, with viral titers in rectal swab samples peaking at 2.22×108 viral RNA copies/mL. Viral capsid proteins were detected in enterocytes within the small intestines on post-infection days (PIDs) 7 and 14. Additionally, viral RNA was detected in intestinal and extra-intestinal tissues, including the central nervous system, the lung and cardiac muscle. The infected neonatal gnotobiotic pigs developed fever, forelimb weakness, rapid breathing and some hand, foot and mouth disease symptoms. Flow cytometry analysis revealed increased frequencies of both CD4+ and CD8+ IFN-γ-producing T cells in the brain and the blood on PID 14, but reduced frequencies were observed in the lung. Furthermore, high titers of serum virus-neutralizing antibodies were generated in both orally and combined oral–nasally infected pigs on PIDs 7, 14, 21 and 28. Together, these results demonstrate that neonatal gnotobiotic pigs represent a novel animal model for evaluating vaccines for human enterovirus 71 and for understanding the pathogenesis of this virus and the associated immune responses.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2222-1751Test

الوصول الحر: https://doaj.org/article/983f0d9d45d54e82839649d0571d08e3Test