المؤلفون: Xiaoyue Guan, Lei Zhong, Jinyu Zhang, Jianhong Lu, Meng Yuan, Lili Ye, Jie Min

المصدر: Heliyon, Vol 10, Iss 6, Pp e27560- (2024)

مصطلحات موضوعية: Lactate dehydrogenase to albumin ratio, Mortality, Sepsis, Propensity score matching, MIMIC-IV database, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: Background: Sepsis is a prevalent and severe medical condition which is frequently observed in the intensive care unit (ICU). Although numerous biomarkers have been identified to predict the prognosis of sepsis, the lactate dehydrogenase to albumin ratio (LDH/ALB ratio) has not been extensively investigated. The principal objective of this study is to assess the relationship between LDH/ALB ratio and all-cause mortality in patients with sepsis. Methods: This study included all adult critically ill patients with sepsis from the Medical Information Mart for Intensive Care IV (MIMIC-IV, version 2.0) database. Propensity score matching (PSM) analysis was conducted to mitigate bias, and Kaplan-Meier curves were performed to evaluate the cumulative survival across different groups. The association between the LDH/ALB ratio and mortality was examined through restricted cubic spline (RCS) analysis and Cox regression analysis. The robustness of the findings was confirmed through subgroup analyses. Additionally, the prognostic capability of the LDH/ALB ratio was further evaluated using receiver operating characteristic (ROC) curve analysis. Results: There were 6059 adult patients with sepsis enrolled in the final analysis. RCS revealed a non-linear relationship between the LDH/ALB ratio and an increased risk of ICU all-cause mortality (χ2 = 46.900, P

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844024035916Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/19ad56822c3e4bb683df8559a0d34355Test

المؤلفون: Xiaoyue Guan, Yuting Wang, Wenlan Li, Wenli Mu, Yifei Tang, Mingfei Wang, Abdelrahman Seyam, Yao Yang, Lifei Pan, Tiezhou Hou

المصدر: International Journal of Molecular Sciences, Vol 25, Iss 7, p 3854 (2024)

مصطلحات موضوعية: apical periodontitis, macrophage efferocytosis, animal model, ARA290, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: Macrophages (Mφs) play a crucial role in the homeostasis of the periapical immune micro-environment caused by bacterial infection. Mφ efferocytosis has been demonstrated to promote the resolution of multiple infected diseases via accelerating Mφ polarization into M2 type. However, the Mφ efferocytosis–apical periodontitis (AP) relationship has not been elucidated yet. This study aimed to explore the role of Mφ efferocytosis in the pathogenesis of AP. Clinical specimens were collected to determine the involvement of Mφ efferocytosis in the periapical region via immunohistochemical and immunofluorescence staining. For a further understanding of the moderator effect of Mφ efferocytosis in the pathogenesis of AP, both an in vitro AP model and in vivo AP model were treated with ARA290, a Mφ efferocytosis agonist. Histological staining, micro-ct, flow cytometry, RT-PCR and Western blot analysis were performed to detect the inflammatory status, alveolar bone loss and related markers in AP models. The data showed that Mφ efferocytosis is observed in the periapical tissues and enhancing the Mφ efferocytosis ability could effectively promote AP resolution via facilitating M2 Mφ polarization. Collectively, our study demonstrates the functional importance of Mφ efferocytosis in AP pathology and highlights that accelerating Mφ efferocytosis via ARA290 could serve as an adjuvant therapeutic strategy for AP.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1422-0067/25/7/3854Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/f3264ea6603649b88a57a008d162309bTest

المؤلفون: Zhibin Liu, Yao Liu, Mingming Liu, Qingjia Gong, Anjie Shi, Xiuhong Li, Xu Bai, Xiaoyue Guan, Bing Hao, Feila Liu, Xing Zhou, Hongfeng Yuan

المصدر: Frontiers in Immunology, Vol 13 (2022)

مصطلحات موضوعية: PD-L1, T cell, orbital fibroblast, CD40, thyroid associated ophthalmopathy, Immunologic diseases. Allergy, RC581-607

الوصف: Thyroid associated ophthalmopathy (TAO), characterized by T cell infiltration and orbital fibroblast activation, is an organ-specific autoimmune disease which is still short of effective and safety therapeutic drugs. The PD-1/PD-L1 pathway has been reported hindering the progression of Graves’ disease to some extent by inhibiting T cell activity, and tumor therapy with a PD-1 inhibitor caused some adverse effects similar to the symptoms of TAO. These findings suggest that the PD-1/PD-L1 pathway may be associated with the pathogenesis of TAO. However, it remains unknown whether the PD-1/PD-L1 pathway is involved in orbital fibroblast activation. Here, we show that orbital fibroblasts from patients with TAO do not express PD-L1. Based on in vitro OF-T cell co-culture system, exogenous PD-L1 weakens T cell-induced orbital fibroblast activation by inhibiting T cell activity, resulting in reduced production of sICAM-1, IL-6, IL-8, and hyaluronan. Additionally, exogenous PD-L1 treatment also inhibits the expression of CD40 and the phosphorylation levels of MAPK and NF-κB pathways in orbital fibroblasts of the OF-T cell co-culture system. Knocking down CD40 with CD40 siRNA or down-regulating the phosphorylation levels of MAPK and NF-κB pathways with SB203580, PD98059, SP600125, and PDTC can both reduce the expression of these cytokines and hyaluronan. Our study demonstrates that the orbital immune tolerance deficiency caused by the lack of PD-L1 in orbital fibroblasts may be one of the causes for the active orbital inflammation in TAO patients, and the utilization of exogenous PD-L1 to reconstruct the orbital immune tolerance microenvironment may be a potential treatment strategy for TAO.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fimmu.2022.849480/fullTest; https://doaj.org/toc/1664-3224Test

الوصول الحر: https://doaj.org/article/4d280b37b03b4f4184a62fb837194ecdTest

المؤلفون: HaiQun Huang, HaiYan Dong, XiaoYue Guan, Li Zhang, Qing Zhou

المصدر: Worldviews on Evidence-Based Nursing. 19:467-476

مصطلحات موضوعية: Stress Disorders, Post-Traumatic, Intensive Care Units, Critical Care, Humans, Family, General Medicine, Respiration, Artificial, General Nursing

الوصف: Adverse psychological outcomes are prevalent among family members of intensive care unit (ICU) patients. The facilitated sensemaking model (FSM) provides a framework for understanding how intensive care nurses can help these family members overcome situations in which a loved one is critically ill, and reduce adverse psychological outcomes through the facilitated sensemaking process.This study aimed to implement FSM-based research performed by ICU nurses and patients' family members to investigate the impact of the facilitated sensemaking intervention on the psychological status of ICU families.The intervention was performed by nurses on 80 family members of mechanically ventilated patients, 40 in the control group and 40 in the experimental group. The control group only received routine medical services, while the experimental group received the nursing intervention based on FSM in addition to routine medical services. Anxiety, depression, and post-traumatic stress disorder (PTSD) were measured with the Self-Rating Anxiety Scale, Self-Rating Depression Scale, and Post-Traumatic Stress Disorder Check-List-Civilian Version (PCL-C), respectively. SPSS version 25.0 was applied to analyze the data; what is more, some statistical methods, including descriptive statistical analysis, chi-square test and t-test were further adopted.Before the intervention, there were no significant differences in anxiety, depression, and PTSD of family members of ICU mechanical ventilation patients between the two groups (p .05). After the intervention, the score of anxiety, depression, and PTSD of family members in the control group and the experimental group were 41.50 ± 5.738 versus 36.50 ± 4.385, p .001; 45.28 ± 8.089 versus 42.13 ± 5.725, p .05; and 30.55 ± 7.595 versus 27.55 ± 4.696, p .05, respectively. The nursing intervention based on FSM significantly alleviated anxiety, depression, and PTSD of mechanical ventilation patients' family members.The nursing intervention based on FSM significantly alleviated anxiety, depression, and PTSD of mechanical ventilation patients' family members. However, there was only a statistically significant difference in the avoidance and numbness symptom cluster of PTSD via the PCL-C. Therefore, the observation time after the implementation of the FSM intervention needs to be extended in the future to clarify the effect of the intervention. Further efforts by advanced practice nurses and the cooperation of patients' families are required to incorporate this intervention into ICU practice.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca51bf29009bb63a4b944323280f44bfTest
https://doi.org/10.1111/wvn.12606Test

المؤلفون: Guotong Ren, Zhenzhen Wang, Yuanbo Lin, Tsubasa Onishi, Xiaoyue Guan, Xian-Huan Wen

المصدر: Day 1 Tue, March 28, 2023.

الوصف: In this work, we study a waterflood field containing over 1,000 wells and the modern field management techniques with full-fidelity 3D geo-cellular reservoir models become computationally prohibitive. To overcome the difficulty, we developed a novel flow-network data-driven model, GPSNet, and used it for rapid history matching and optimization. GPSNet includes physics, such as mass conservation, multiphase flow, phase changes, etc., while maintaining a good level of efficiency. To build such a model, a cluster of 1-D connections among well completion points are constructed and form a flow network. Multi-phase fluid flow is assumed to occur in each 1-D connection and the flow in the whole network is simulated by our in-house general-purpose simulator. Next, to effectively reduce the uncertainty, a hierarchical history-matching workflow is adopted to match the production data. Ensemble Smoother with Multiple Data Assimilation (ESMDA) is utilized to reduce the error at each step of the history matching. Next, a best-matched candidate is selected for numerical optimization to maximize oil production rates with constraints satisfying field conditions. Excellent history-matching results have been achieved on the field level and good matches have also been observed for key producers. In addition, the history matching consumes mere 4 hours to finish 1,100 simulation jobs. The successful application of the GPSNet to this waterflood field demonstrates a promising workflow that can be used as a fast and reliable decision-making tool for reservoir management.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::bcc67d8ce5a0477f3221be834ea8f44eTest
https://doi.org/10.2118/212188-msTest

المؤلفون: Zhenzhen Wang, Xiaoyue Guan, William Milliken, Xian-Huan Wen

المصدر: SPE Journal. 27:2033-2051

مصطلحات موضوعية: Energy Engineering and Power Technology, Geotechnical Engineering and Engineering Geology

الوصف: Summary Full-fidelity models can be computationally expensive during history matching (HM) and robust optimization as these problems typically require hundreds of simulations. Previously, we have implemented a physics-based data-driven flow network model, general purpose simulator-powered network model (GPSNet), that serves as a surrogate without the need to build the 3D full-fidelity model. In this paper, GPSNet is enhanced to GPSNet-2D to better capture thermal processes, especially gravity drainage. This enhancement successfully enables a rapid HM and robust optimization for a steamflood sector model. In GPSNet-2D, the reservoir is discretized into a series of 2D connections (x-z planes) between well completions. These connections capture the main areal/vertical flow paths, and the grid properties of each connection are interpreted with historical data. Steam segregation and heat loss are included to better represent the subsurface physics. When simulating GPSNet-2D using a commercial simulator, an equivalent 3D Cartesian model is designed where vertical slices correspond to the interwell connection planes. Thereafter, an iterative HM is conducted using the ensemble smoother with multiple data assimilation. The best-matched model is then used for steam injector control optimization. The GPSNet-2D model is first validated through a synthetic steamflood case. HM result shows that the GPSNet-2D model not only aligns closely to field-scale volumetric data but also yields good well-level matches, including bottomhole pressure (BHP)/bottomhole temperature (BHT) and phase rates. Then, GPSNet-2D is successfully applied to steamflood HM and robust optimization for a 36-well sector of a heavy oil field in the San Joaquin Valley in North America. Again, the calibrated GPSNet-2D model demonstrates its capability and reliability by generating accurate field-scale match results and reasonable matches for most of the wells. For robust well control optimization, we select nine realizations from the posterior ensemble and maximize NPV under well constraints simultaneously. The optimal case improves the average NPV by 27% over the reference case. The integration with a commercial simulator in GPSNet-2D provides flexibility to account for complex physics in the thermal recovery processes, such as steam segregation near injectors, fast steam breakthroughs at producers, and heat loss to the overburden/underburden formations. Unlike traditional simulation that relies on a detailed characterization of geological models, the GPSNet-2D model only requires well volumetric production/injection data and its approximate trajectory and can be generated and updated rapidly. In addition, GPSNet-2D also runs much faster (minutes) than a full-fidelity thermal model due to having much fewer gridblocks in the model. To our knowledge, this is the first time a physics-based data-driven network model integrated with a commercial simulator is demonstrated via a field steamflood case. Unlike approaches developed with analytic/empirical solutions or research simulators, the use of a commercial simulator makes it possible to extend flow network modeling to simulate enhanced oil recovery processes more realistically. It serves as an ideal surrogate model for both fast and reliable decision-making in reservoir management.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::4f9f943046b036498e27d8889369ec12Test
https://doi.org/10.2118/209611-paTest

المؤلفون: Xiaoyue Guan, Yani He, Yingxue Li, Chen Shi, Zhichen Wei, Rui Zhao, Yue Han, Lifei Pan, Jianmin Yang, Tiezhou Hou

المصدر: Journal of Periodontology. 93:1589-1602

مصطلحات موضوعية: Inflammation, Osteogenesis, Periodontal Ligament, Alveolar Bone Loss, NF-kappa B, Animals, Humans, Periodontics, Cell Differentiation, Periodontitis, Cells, Cultured, Rats, Signal Transduction

الوصف: Gremlin has been reported to regulate inflammation and osteogenesis. Periodontitis is a destructive disease degenerating periodontal tissues, therefore leads to alveolar bone resorption and tooth loss. Based on the importance of Gremlin's bio-activity, the aim of this study is to, in vivo and in vitro, unveil the function of Gremlin in regulating the development of periodontitis and its consequent effects on alveolar bone loss.Clinical specimens were used to determine the expression of Gremlin in periodontal tissues by immunohistochemical staining and western blot. Then utilizing the rat periodontitis model to investigate the function of gremlin-regulated nuclear factor-kappa B (NF-κB) pathway during the development of periodontal inflammation and the alveolar bone loss. Last, the regulation of the osteogenesis of human periodontal ligament stem cells (hPDLSCs) by Gremlin under inflamed condition was analyzed by alkaline phosphatase (ALP) and alizarin red staining (ARS).We found clinically and experimentally that the expression of Gremlin is markedly increased in periodontitis tissues. Interestingly, we revealed that Gremlin regulated the progress of periodontitis via regulating the activities of NF-κB pathway and interleukin-1β (IL-1β). Notably, we observed that Gremlin influenced the osteogenesis of hPDLSCs. Thus, our present study identified Gremlin as a new key regulator for development of periodontitis.Our current study illustrated that Gremlin acts as a crucial mediator and possibly serves as a potential diagnostic marker for periodontitis. Discovery of new factors involved in the pathophysiology of periodontitis could contribute to the development of novel therapeutic treatment for the disease.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c1fdcf14897fb06a18a598792a8e953Test
https://doi.org/10.1002/jper.21-0474Test

المؤلفون: Yani He, Yuting Wang, Xiangbin Jia, Yingxue Li, Yao Yang, Lifei Pan, Rui Zhao, Yue Han, Feng Wang, Xiaoyue Guan, Tiezhou Hou

المصدر: International Immunopharmacology. 119:110192

مصطلحات موضوعية: Pharmacology, Immunology, Immunology and Allergy

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::82737dd105936b1c207edf70a6c9edb2Test
https://doi.org/10.1016/j.intimp.2023.110192Test

المؤلفون: Yingxue Li, Yao Yang, Xiaoyue Guan, Zhijun Liu, Lifei Pan, Yuting Wang, Xiangbin Jia, Jianmin Yang, Tiezhou Hou

الإتاحة: https://doi.org/10.1111/odi.14944/v2/response1Test

المؤلفون: Yingxue Li, Xiaoyue Guan, Yani He, Xiangbin Jia, Lifei Pan, Yuting Wang, Yue Han, Rui Zhao, Jianmin Yang, Tiezhou Hou

المصدر: SSRN Electronic Journal.

مصطلحات موضوعية: History, Polymers and Plastics, Business and International Management, Industrial and Manufacturing Engineering

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::bc8331dc4851ce6b3a1786767f0bc162Test
https://doi.org/10.2139/ssrn.4261737Test