المؤلفون: Jingdong Chen, Heping Wan, Huixia Zhao, Xigang Dai, Wanjin Wu, Jin Liu, Jinsong Xu, Rui Yang, Benbo Xu, Changli Zeng, Xuekun Zhang

المصدر: BMC Plant Biology, Vol 24, Iss 1, Pp 1-14 (2024)

مصطلحات موضوعية: Brassica napus, XTH gene family, Abiotic stress tolerance, Expression profiling, Botany, QK1-989

الوصف: Abstract XTH genes are key genes that regulate the hydrolysis and recombination of XG components and plays role in the structure and composition of plant cell walls. Therefore, clarifying the changes that occur in XTHs during plant defense against abiotic stresses is informative for the study of the plant stress regulatory mechanism mediated by plant cell wall signals. XTH proteins in Arabidopsis thaliana was selected as the seed sequences in combination with its protein structural domains, 80 members of the BnXTH gene family were jointly identified from the whole genome of the Brassica napus ZS11, and analyzed for their encoded protein physicochemical properties, phylogenetic relationships, covariance relationships, and interoperating miRNAs. Based on the transcriptome data, the expression patterns of BnXTHs were analyzed in response to different abiotic stress treatments. The relative expression levels of some BnXTH genes under Al, alkali, salt, and drought treatments after 0, 6, 12 and 24 h were analyzed by using qRT-PCR to explore their roles in abiotic stress tolerance in B. napus. BnXTHs showed different expression patterns in response to different abiotic stress signals, indicating that the response mechanisms of oilseed rape against different abiotic stresses are also different. This paper provides a theoretical basis for clarifying the function and molecular genetic mechanism of the BnXTH gene family in abiotic stress tolerance in rapeseed.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2229Test

الوصول الحر: https://doaj.org/article/fb4f728110a140c1ac8d27201919abe5Test

المؤلفون: Junjie Han, Yichen Liu, Yiting Shen, Weihua Li

المصدر: International Journal of Molecular Sciences; Volume 24; Issue 12; Pages: 9886

مصطلحات موضوعية: wheat, XTH gene family, drought, root plasticity, transgenic

جغرافية الموضوع: agris

الوصف: Drought has become a major limiting factor for wheat productivity, and its negative impact on crop growth is anticipated to increase with climate deterioration in arid areas. Xyloglucan endoglycosylases/hydrolases (XTHs) are involved in constructing and remodeling cell wall structures and play an essential role in regulating cell wall extensibility and stress responses. However, there are no systematic studies on the wheat XTH gene family. In this study, 71 wheat XTH genes (TaXTHs) were characterized and classified into three subgroups through phylogenetic analysis. Genomic replication promoted the expansion of TaXTHs. We found a catalytically active motif and a potential N-linked glycosylation domain in all TaXTHs. Further expression analysis revealed that many TaXTHs in the roots and shoots were significantly associated with drought stress. The wheat TaXTH12.5a gene was transferred into Arabidopsis to verify a possible role of TaXTHs in stress response. The transgenic plants possessed higher seed germination rates and longer roots and exhibited improved tolerance to drought. In conclusion, bioinformatics and gene expression pattern analysis indicated that the TaXTH genes played a role in regulating drought response in wheat. The expression of TaXTH12.5a enhanced drought tolerance in Arabidopsis and supported the XTH genes’ role in regulating drought stress response in plants.

وصف الملف: application/pdf

العلاقة: Molecular Plant Sciences; https://dx.doi.org/10.3390/ijms24129886Test

الإتاحة: https://doi.org/10.3390/ijms24129886Test

المؤلفون: Richard John Tiika, Jia Wei, Guangxin Cui, Yanjun Ma, Hongshan Yang, Huirong Duan

المصدر: BMC Plant Biology, Vol 21, Iss 1, Pp 1-15 (2021)

مصطلحات موضوعية: Salicornia europaea, PacBio Iso-Seq, XTH gene family, Salinity, Drought, Botany, QK1-989

الوصف: Abstract Background Salicornia europaea is a halophyte that has a very pronounced salt tolerance. As a cell wall manipulating enzyme, xyloglucan endotransglycosylase/hydrolase (XTH) plays an important role in plant resistance to abiotic stress. However, no systematic study of the XTH gene family in S. europaea is well known. PacBio Iso-Seq transcriptome sequence data were used for bioinformatics and gene expression analysis using real-time quantitative polymerase chain reaction (RT-qPCR). Results Transcriptome sequencing (PacBio Iso-Seq system) generated 16,465,671 sub-reads and after quality control of Iso-Seq, 29,520 isoforms were obtained with an average length of 2112 bp. A total of 24,869 unigenes, with 98% of which were obtained using coding sequences (CDSs), and 6398 possible transcription factors (TFs) were identified. Thirty-five (35) non-redundant potential SeXTH proteins were identified in S. europaea and categorized into group I/II and group III based on their genetic relatedness. Prediction of the conserved motif revealed that the DE(I/L/F/V)DF(I)EFLG domain was conserved in the S. europaea proteins and a potential N-linked glycosylation domain N(T)V(R/L/T/I)T(S/K/R/F/P)G was also located near the catalytic residues. All SeXTH genes exhibited discrete expression patterns in different tissues, at different times, and under different stresses. For example, 27 and 15 SeXTH genes were positively expressed under salt stress in shoots and roots at 200 mM NaCl in 24 h, and 34 SeXTH genes were also positively regulated under 48 h of drought stress in shoots and roots. This indicates their function in adaptation to salt and drought stress. Conclusion The present study discovered SeXTH gene family traits that are potential stress resistance regulators in S. europaea, and this provides a basis for future functional diversity research.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2229Test

الوصول الحر: https://doaj.org/article/0b99b125c0f649c5a93fe4be77475978Test

المؤلفون: Zhongyi Yang, Rui Zhang, Zhichun Zhou

المصدر: Frontiers in Plant Science, Vol 13 (2022)

مصطلحات موضوعية: XTH gene family, cell wall remodeling, Schima superba, genome-wide identification, expression analysis, functional interaction network, Plant culture, SB1-1110

الوصف: Xyloglucan endotransglucosylase/hydrolase (XTH), belonging to glycoside hydrolase family 16, is one of the key enzymes in plant cell wall remodeling. Schima superba is an important timber and fireproof tree species in southern China. However, little is known about XTHs in S. superba. In the present study, a total of 34 SsuXTHs were obtained, which were classified into three subfamilies based on the phylogenetic relationship and unevenly distributed on 18 chromosomes. Furthermore, the intron–exon structure and conserved motif composition of them supported the classification and the members belonging to the same subfamily shared similar gene structures. Segmental and tandem duplication events did not lead to SsuXTH gene family expansion, and strong purifying selection pressures during evolution led to similar structure and function of SsuXTH gene family. The interaction network and cis-acting regulatory elements analysis revealed the SsuXTH expression might be regulated by multiple hormones, abiotic stresses and transcription factors. Finally, expression profiles and GO enrichment analysis showed most of the tandem repeat genes were mainly expressed in the phloem and xylem and they mainly participated in glycoside metabolic processes through the transfer and hydrolysis of xyloglucan in the cell wall and then regulated fiber elongation.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fpls.2022.911761/fullTest; https://doaj.org/toc/1664-462XTest

الوصول الحر: https://doaj.org/article/26abea0ffd9146d2a2767b2b53d4166fTest

المؤلفون: Yu-Shen Ma, Hong-Dong Jie, Long Zhao, Xue-Ying Lv, Xiao-Chun Liu, Yan-Yi Tang, Ying Zhang, Peng-Liang He, Hu-Cheng Xing, Yu-Cheng Jie

المصدر: International Journal of Molecular Sciences, Vol 23, Iss 24, p 16104 (2022)

مصطلحات موضوعية: Boehmeria nivea, XTH gene family, genome-wide identification, Cd stress, expression analysis, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: Xyloglucan endotransglycosylase/hydrolase (XTH) genes play an important role in plant resistance to abiotic stress. However, systematic studies of the response of Boehmeria nivea (ramie) XTH genes (BnXTHs) to cadmium (Cd) stress are lacking. We sought to identify the BnXTH-family genes in ramie through bioinformatics analyses and to investigate their responses to Cd stress. We identified 19 members of the BnXTH gene family from the ramie genome, referred to as BnXTH1-19, among which BnXTH18 and BnXTH19 were located on no chromosomes and the remaining genes were unevenly distributed across 11 chromosomes. The 19 members were divided into four groups, Groups I/II/IIIA/IIIB, according to their phylogenetic relationships, and these groups were supported by analyses of intron–exon structure and conserved motif composition. A highly conserved catalytic site (HDEIDFEFLG) was observed in all BnXTH proteins. Additionally, three gene pairs (BnXTH6–BnXTH16, BnXTH8–BnXTH9, and BnXTH17–BnXTH18) were obtained with a fragment and tandem-repeat event analysis of the ramie genome. An analysis of cisregulatory elements revealed that BnXTH expression might be regulated by multiple hormones and abiotic and biotic stress responses. In particular, 17 cisregulatory elements related to abiotic and biotic stress responses and 11 cisregulatory elements related to hormone responses were identified. We also found that most BnXTH genes responded to Cd stress, and BnXTH1, BnXTH3, BnXTH6, and BnXTH15 were most likely to contribute to the Cd tolerance of ramie, as evidenced by the substantial increases in expression under Cd treatment. Heterologous expression of BnXTH1, BnXTH6, and BnXTH15 significantly enhanced the Cd tolerance of transgenic yeast cells. These results suggest that the BnXTH gene family is involved in Cd stress responses, laying a theoretical foundation for functional studies of BnXTH genes and the innovative breeding of Cd-tolerant ramie.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1422-0067/23/24/16104Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/0d263b31f7604932b51addf18098ae16Test

المؤلفون: Richard John Tiika (11605178), Jia Wei (120974), Guangxin Cui (11605180), Yanjun Ma (621886), Hongshan Yang (11605182), Huirong Duan (11605184)

مصطلحات موضوعية: Genetics, Salicornia europaea, PacBio Iso-Seq, XTH gene family, Salinity, Drought

الوصف: Additional file 1: Supplementary file 1. XTH sequences from A. thaliana, N. tabacum and M. truncatula used for phylogenetic tree construction.

العلاقة: https://figshare.com/articles/journal_contribution/Additional_file_1_of_Transcriptome-wide_characterization_and_functional_analysis_of_Xyloglucan_endo-transglycosylase_hydrolase_XTH_gene_family_of_Salicornia_europaea_L_under_salinity_and_drought_stress/16869766Test

الإتاحة: https://doi.org/10.6084/m9.figshare.16869766.v1Test

المؤلفون: Richard John Tiika (11605178), Jia Wei (120974), Guangxin Cui (11605180), Yanjun Ma (621886), Hongshan Yang (11605182), Huirong Duan (11605184)

مصطلحات موضوعية: Genetics, Salicornia europaea, PacBio Iso-Seq, XTH gene family, Salinity, Drought

الوصف: Additional file 4: Supplementary file 4. Amino acid sequence of the predicted 35 SeXTH proteins.

العلاقة: https://figshare.com/articles/journal_contribution/Additional_file_4_of_Transcriptome-wide_characterization_and_functional_analysis_of_Xyloglucan_endo-transglycosylase_hydrolase_XTH_gene_family_of_Salicornia_europaea_L_under_salinity_and_drought_stress/16869772Test

الإتاحة: https://doi.org/10.6084/m9.figshare.16869772.v1Test

المؤلفون: Li, Junjie Han, Yichen Liu, Yiting Shen, Weihua

المصدر: International Journal of Molecular Sciences; Volume 24; Issue 12; Pages: 9886

مصطلحات موضوعية: wheat, XTH gene family, drought, root plasticity, transgenic

الوصف: Drought has become a major limiting factor for wheat productivity, and its negative impact on crop growth is anticipated to increase with climate deterioration in arid areas. Xyloglucan endoglycosylases/hydrolases (XTHs) are involved in constructing and remodeling cell wall structures and play an essential role in regulating cell wall extensibility and stress responses. However, there are no systematic studies on the wheat XTH gene family. In this study, 71 wheat XTH genes (TaXTHs) were characterized and classified into three subgroups through phylogenetic analysis. Genomic replication promoted the expansion of TaXTHs. We found a catalytically active motif and a potential N-linked glycosylation domain in all TaXTHs. Further expression analysis revealed that many TaXTHs in the roots and shoots were significantly associated with drought stress. The wheat TaXTH12.5a gene was transferred into Arabidopsis to verify a possible role of TaXTHs in stress response. The transgenic plants possessed higher seed germination rates and longer roots and exhibited improved tolerance to drought. In conclusion, bioinformatics and gene expression pattern analysis indicated that the TaXTH genes played a role in regulating drought response in wheat. The expression of TaXTH12.5a enhanced drought tolerance in Arabidopsis and supported the XTH genes’ role in regulating drought stress response in plants.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=multidiscipl::149485a549e4ba5b3c1bf86981ca9178Test

المؤلفون: Li Song, Babu Valliyodan, Silvas Prince, Jinrong Wan, Henry T. Nguyen

المصدر: International Journal of Molecular Sciences; Volume 19; Issue 9; Pages: 2705

مصطلحات موضوعية: Glycine max, XTH gene family, transgenic soybean, plant genome, plant hormone, flooding, root plasticity

جغرافية الموضوع: agris

الوصف: Xyloglucan endotransglycosylases/hydrolases (XTHs) are a class of enzymes involved in the construction and remodeling of cellulose/xyloglucan crosslinks and play an important role in regulating cell wall extensibility. However, little is known about this class of enzymes in soybean. Here, 61 soybean XTH genes (GmXTHs) were identified and classified into three subgroups through comparative phylogenetic analysis. Genome duplication greatly contributed to the expansion of GmXTH genes in soybean. A conserved amino acid motif responsible for the catalytic activity was identified in all GmXTHs. Further expression analysis revealed that most GmXTHs exhibited a distinct organ-specific expression pattern, and the expression level of many GmXTH genes was significantly associated with ethylene and flooding stress. To illustrate a possible role of XTH genes in regulating stress responses, the Arabidopsis AtXTH31 gene was overexpressed in soybean. The generated transgenic plants exhibited improved tolerance to flooding stress, with a higher germination rate and longer roots/hypocotyls during the seedling stage and vegetative growth stages. In summary, our combined bioinformatics and gene expression pattern analyses suggest that GmXTH genes play a role in regulating soybean stress responses. The enhanced soybean flooding tolerance resulting from the expression of an Arabidopsis XTH also supports the role of XTH genes in regulating plant flooding stress responses.

وصف الملف: application/pdf

العلاقة: Molecular Plant Sciences; https://dx.doi.org/10.3390/ijms19092705Test

الإتاحة: https://doi.org/10.3390/ijms19092705Test

المؤلفون: Zhongyi Yang, Rui Zhang, Zhichun Zhou

مصطلحات موضوعية: Botany, Plant Biology, Plant Systematics and Taxonomy, Plant Cell and Molecular Biology, Plant Developmental and Reproductive Biology, Plant Pathology, Plant Physiology, Plant Biology not elsewhere classified, XTH gene family, cell wall remodeling, Schima superba, genome-wide identification, expression analysis, functional interaction network

الوصف: Xyloglucan endotransglucosylase/hydrolase (XTH), belonging to glycoside hydrolase family 16, is one of the key enzymes in plant cell wall remodeling. Schima superba is an important timber and fireproof tree species in southern China. However, little is known about XTHs in S. superba. In the present study, a total of 34 SsuXTHs were obtained, which were classified into three subfamilies based on the phylogenetic relationship and unevenly distributed on 18 chromosomes. Furthermore, the intron–exon structure and conserved motif composition of them supported the classification and the members belonging to the same subfamily shared similar gene structures. Segmental and tandem duplication events did not lead to SsuXTH gene family expansion, and strong purifying selection pressures during evolution led to similar structure and function of SsuXTH gene family. The interaction network and cis-acting regulatory elements analysis revealed the SsuXTH expression might be regulated by multiple hormones, abiotic stresses and transcription factors. Finally, expression profiles and GO enrichment analysis showed most of the tandem repeat genes were mainly expressed in the phloem and xylem and they mainly participated in glycoside metabolic processes through the transfer and hydrolysis of xyloglucan in the cell wall and then regulated fiber elongation.

العلاقة: https://figshare.com/articles/dataset/Data_Sheet_1_The_XTH_Gene_Family_in_Schima_superba_Genome-Wide_Identification_Expression_Profiles_and_Functional_Interaction_Network_Analysis_ZIP/20077769Test

الإتاحة: https://doi.org/10.3389/fpls.2022.911761.s001Test
https://figshare.com/articles/dataset/Data_Sheet_1_The_XTH_Gene_Family_in_Schima_superba_Genome-Wide_Identification_Expression_Profiles_and_Functional_Interaction_Network_Analysis_ZIP/20077769Test