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1دورية أكاديمية
المؤلفون: Bramswig, NC, Ludecke, HJ, Pettersson, M, Albrecht, B, Bernier, RA, Cremer, K, Eichler, EE, Falkenstein, D, Gerdts, J, Jansen, S, Kuechler, A, Kvarnung, M, Lindstrand, A, Nilsson, D, Nordgren, A, Pfundt, R, Spruijt, L, Surowy, HM, de Vries, BBA, Wieland, T, Engels, H, Strom, TM, Kleefstra, T, Wieczorek, D
المصدر: Human genetics. 136(2):179-192
مصطلحات موضوعية: Medicin och hälsovetenskap
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2دورية أكاديمية
المؤلفون: Arnold, C, Feldner, A, Pfisterer, L, Hodebeck, M, Troidl, K, Genove, G, Wieland, T, Hecker, M, Korff, T
المصدر: EMBO molecular medicine. 6(8):1075-1089
مصطلحات موضوعية: Medicin och hälsovetenskap
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3دورية أكاديمية
المؤلفون: Makwana, M., dos Santos Souza, C., Pickup, B.T., Thompson,, M.J., Lomada, S.K., Feng, Y., Wieland, T., Jackson, R.F.W., Muimo, R.
الوصف: Non-hydrolysable stable analogues of τ-phosphohistidine (τ-pHis) and π-pHis have been designed aided by electrostatic surface potential calculations, and subsequently synthesized. The τ-pHis and π-pHis analogues (phosphopyrazole 8 and pyridyl amino amide 13, respectively) were used as haptens to generate pHis polyclonal antibodies. Both τ-pHis and π-pHis conjugates in the form of a BSA-glutaraldehyde-τ-pHis and BSA-glutaraldehyde-π-pHis were synthesized and characterized by 31P NMR spectroscopy. Commercially available τ-pHis (SC56-2) and π-pHis (SC1-1; SC50-3) monoclonal antibodies were used to show that the BSA-G-τ-pHis and BSA-G-π-pHis conjugates could be used to assess the selectivity of pHis antibodies in a competitive ELISA. Subsequently, the selectivity of the generated pHis antibodies generated using phosphopyrazole 8 and pyridyl amino amide 13 as haptens was assessed by competitive ELISA against His, pSer, pThr, pTyr, τ-pHis and π-pHis. Antibodies generated using the phosphopyrazole 8 as a hapten were found to be selective for τ-pHis, and antibodies generated using the pyridyl amino amide 13 were found to be selective for π-pHis. Both τ- and π-pHis antibodies were shown to be effective in immunological experiments, including ELISA, western blot, and immunofluorescence. The τ-pHis antibody was also shown to be useful in the immunoprecipitation of proteins containing pHis.
وصف الملف: text
العلاقة: https://eprints.whiterose.ac.uk/201847/1/ChemBioChem%20-%202023%20-%20Makwana%20-%20Chemical%20Tools%20for%20Studying%20Phosphohistidine%20%20Generation%20of%20Selective%20%20%E2%80%90Phosphohistidine%20and.pdfTest; Makwana, M., dos Santos Souza, C. orcid.org/0000-0001-5314-6263 , Pickup, B.T. orcid.org/0000-0002-2728-5486 et al. (6 more authors) (2023) Chemical tools for study of phosphohistidine: generation of selective Τ‐phosphohistidine and Π‐phosphohistidine antibodies. ChemBioChem. e202300182. ISSN 1439-4227
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4دورية أكاديمية
المصدر: The Wilson Journal of Ornithology, 2019 Dec 01. 131(4), 817-824.
الوصول الحر: https://www.jstor.org/stable/27014211Test
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5دورية أكاديمية
المؤلفون: Dobrindt, U. (Ulrich), Wami, H.T. (Haleluya), Schmidt-Wieland, T. (Torsten), Bertsch, D. (Daniela), Oberdorfer, K. (Klaus), Hof, H. (Herbert)
المساهمون: Universitäts- und Landesbibliothek Münster
مصطلحات موضوعية: parallel resistance, resistance plasmid, horizontal gene transfer, prophylaxis of uncomplicated UTI, ddc:610, info:eu-repo/classification/ddc/610, Medicine and health
الوصف: The resistance of uropathogens to various antibiotics is increasing, but nitroxoline remains active in vitro against some relevant multidrug resistant uropathogenic bacteria. 'E. coli' strains, which are among the most common uropathogens, are unanimously susceptible. Thus, nitroxoline is an option for the therapy of urinary tract infections caused by multiresistant bacteria. Since nitroxoline is active against bacteria in biofilms, it will also be effective in patients with indwelling catheters or foreign bodies in the urinary tract. Cotrimoxazole, on the other hand, which, in principle, can also act on bacteria in biofilms, is frequently inactive against multiresistant uropathogens. Based on phenotypic resistance data from a large number of urine isolates, structural characterisation of an MDR plasmid of a recent ST131 uropathogenic 'E. coli' isolate, and publicly available genomic data of resistant enterobacteria, we show that nitroxoline could be used instead of cotrimoxazole for intervention against MDR uropathogens. Particularly in uropathogenic 'E. coli', but also in other enterobacterial uropathogens, the frequent parallel resistance to different antibiotics due to the accumulation of multiple antibiotic resistance determinants on mobile genetic elements argues for greater consideration of nitroxoline in the treatment of uncomplicated urinary tract infections.
وصف الملف: application/pdf
العلاقة: info:eu-repo/semantics/reference/url/https://www.mdpi.com/article/10.3390/antibiotics10060645/s1Test; https://nbn-resolving.org/urn:nbn:de:hbz:6-52029478251Test; urn:nbn:de:hbz:6-52029478251; https://miami.uni-muenster.de/Record/d6b397b0-8ad8-4e39-ab00-2f2311694c52Test; https://repositorium.uni-muenster.de/transfer/miami/d6b397b0-8ad8-4e39-ab00-2f2311694c52Test
الإتاحة: https://doi.org/10.17879/62039660944Test
https://doi.org/10.3390/antibiotics10060645Test
https://nbn-resolving.org/urn:nbn:de:hbz:6-52029478251Test
https://miami.uni-muenster.de/Record/d6b397b0-8ad8-4e39-ab00-2f2311694c52Test
https://repositorium.uni-muenster.de/transfer/miami/d6b397b0-8ad8-4e39-ab00-2f2311694c52Test -
6دورية أكاديمية
المؤلفون: Arnold, C, Demirel, E, Feldner, A, Genove, G, Zhang, HJ, Sticht, C, Wieland, T, Hecker, M, Heximer, S, Korff, T
المصدر: FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 32(4):2021-2035
مصطلحات موضوعية: Medicin och hälsovetenskap
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7دورية أكاديمية
المؤلفون: Fuchsberger, C, Flannick, J, Teslovich, TM, Mahajan, A, Agarwala, V, Gaulton, KJ, Ma, C, Fontanillas, P, Moutsianas, L, McCarthy, DJ, Rivas, MA, Perry, JRB, Sim, X, Blackwell, TW, Robertson, NR, Rayner, NW, Cingolani, P, Locke, AE, Tajes, JF, Highland, HM, Dupuis, J, Chines, PS, Lindgren, CM, Hartl, C, Jackson, AU, Chen, H, Huyghe, JR, van de Bunt, M, Pearson, RD, Kumar, A, Muller-Nurasyid, M, Grarup, N, Stringham, HM, Gamazon, ER, Lee, J, Chen, YH, Scott, RA, Below, JE, Chen, P, Huang, J, Go, MJ, Stitzel, ML, Pasko, D, Parker, SCJ, Varga, TV, Green, T, Beer, NL, Day-Williams, AG, Ferreira, T, Fingerlin, T, Horikoshi, M, Hu, C, Huh, I, Ikram, MK, Kim, BJ, Kim, Y, Kim, YJ, Kwon, MS, Lee, S, Lin, KH, Maxwell, TJ, Nagai, Y, Wang, X, Welch, RP, Yoon, J, Zhang, W, Barzilai, N, Voight, BF, Han, BG, Jenkinson, CP, Kuulasmaa, T, Kuusisto, J, Manning, A, Ng, MCY, Palmer, ND, Balkau, B, Stancakova, A, Abboud, HE, Boeing, H, Giedraitis, V, Prabhakaran, D, Gottesman, O, Scott, J, Carey, J, Kwan, P, Grant, G, Smith, JD, Neale, BM, Purcell, S, Butterworth, AS, Howson, JMM, Lee, HM, Lu, YC, Kwak, SH, Zhao, W, Danesh, J, Lam, VKL, Park, KS, Saleheen, D, So, WY, Tam, CHT, Afzal, U, Aguilar, D, Arya, R, Aung, T, Chan, E, Navarro, C, Cheng, CY, Palli, D, Correa, A, Curran, JE, Rybin, D, Farook, VS, Fowler, SP, Freedman, BI, Griswold, M, Hale, DE, Hicks, PJ, Khor, CC, Kumar, S, Lehne, B, Thuillier, D, Lim, WY, Liu, J, van der Schouw, YT, Loh, M, Musani, SK, Puppala, S, Scott, WR, Yengo, L, Tan, ST, Taylor, HA, Thameem, F, Wilson, G, Wong, TY, Njolstad, PR, Levy, JC, Mangino, M, Bonnycastle, LL, Schwarzmayr, T, Fadista, J, Surdulescu, GL, Herder, C, Groves, CJ, Wieland, T, Bork-Jensen, J, Brandslund, I, Christensen, C, Koistinen, HA, Doney, ASF, Kinnunen, L, Esko, T, Farmer, AJ, Hakaste, L, Hodgkiss, D, Kravic, J, Lyssenko, V, Hollensted, M, Jorgensen, ME, Jorgensen, T, Ladenvall, C, Justesen, JM, Karajamaki, A, Kriebel, J, Rathmann, W, Lannfelt, L, Lauritzen, T, Narisu, N, Linneberg, A, Melander, O, Milani, L, Neville, M, Orho-Melander, M, Qi, L, Qi, QB, Roden, M, Rolandsson, O, Swift, A, Rosengren, AH, Stirrups, K, Wood, AR, Mihailov, E, Blancher, C, Carneiro, MO, Maguire, J, Poplin, R, Shakir, K, Fennell, T, DePristo, M, de Angelis, MH, Deloukas, P, Gjesing, AP, Jun, G, Nilsson, P, Murphy, J, Onofrio, R, Thorand, B, Hansen, T, Meisinger, C, Hu, FB, Isomaa, B, Karpe, F, Liang, LM, Peters, A, Huth, C, O'Rahilly, SP, Palmer, CNA, Pedersen, O, Rauramaa, R, Tuomilehto, J, Salomaa, V, Watanabe, RM, Syvanen, AC, Bergman, RN, Bharadwaj, D, Bottinger, EP, Cho, YS, Chandak, GR, Chan, JCN, Chia, KS, Daly, MJ, Ebrahim, SB, Langenberg, C, Elliott, P, Jablonski, KA, Lehman, DM, Jia, WP, Ma, RCW, Pollin, TI, Sandhu, M, Tandon, N, Froguel, P, Barroso, I, Teo, YY, Zeggini, E, Loos, RJF, Small, KS, Ried, JS, DeFronzo, RA, Grallert, H, Glaser, B, Metspalu, A, Wareham, NJ, Walker, M, Banks, E, Gieger, C, Ingelsson, E, Im, HK, Illig, T, Franks, PW, Buck, G, Trakalo, J, Buck, D, Prokopenko, I, Magi, R, Lind, L, Farjoun, Y, Owen, KR, Gloyn, AL, Strauch, K, Tuomi, T, Kooner, JS, Lee, JY, Park, T, Donnelly, P, Morris, AD, Hattersley, AT, Bowden, DW, Collins, FS, Atzmon, G, Chambers, JC, Spector, TD, Laakso, M, Strom, TM, Bell, GI, Blangero, J, Duggirala, R, Tai, ES, McVean, G, Hanis, CL, Wilson, JG, Seielstad, M, Frayling, TM, Meigs, JB, Cox, NJ, Sladek, R, Lander, ES, Gabriel, S, Burtt, NP, Mohlke, KL, Meitinger, T, Groop, L, Abecasis, G, Florez, JC, Scott, LJ, Morris, AP, Kang, HM, Boehnke, M, Altshuler, D, McCarthy, MI
المصدر: Nature. 536(7614):41
مصطلحات موضوعية: Medicin och hälsovetenskap
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8دورية أكاديمية
المؤلفون: Trova, S., Lin, F., Lomada, S., Fenton, M., Chauhan, B., Adams, A., Puri, A., Di Maio, A., Wieland, T., Sewell, D., Dick, K., Wiseman, Daniel H, Wilks, Deepti P, Goodall, M., Drayson, M. T., Khanim, F. L., Bunce, C. M.
المساهمون: School of Biosciences, University of Birmingham, Birmingham, United Kingdom
الوصف: A history of infection has been linked with increased risk of acute myeloid leukaemia (AML) and related myelodysplastic syndromes (MDS). Furthermore, AML and MDS patients suffer frequent infections because of disease-related impaired immunity. However, the role of infections in the development and progression of AML and MDS remains poorly understood. We and others previously demonstrated that the human nucleoside diphosphate kinase (NDPK) NM23-H1 protein promotes AML blast cell survival by inducing secretion of IL-1β from accessory cells. NDPKs are an evolutionary highly conserved protein family and pathogenic bacteria secrete NDPKs that regulate virulence and host-pathogen interactions. Here, we demonstrate the presence of IgM antibodies against a broad range of pathogen NDPKs and more selective IgG antibody activity against pathogen NDPKs in the blood of AML patients and normal donors, demonstrating that in vivo exposure to NDPKs likely occurs. We also show that pathogen derived NDPK-proteins faithfully mimic the catalytically independent pro-survival activity of NM23-H1 against primary AML cells. Flow cytometry identified that pathogen and human NDPKs selectively bind to monocytes in peripheral blood. We therefore used vitamin D3 differentiated monocytes from wild type and genetically modified THP1 cells as a model to demonstrate that NDPK-mediated IL-1β secretion by monocytes is NLRP3-inflammasome and caspase 1 dependent, but independent of TLR4 signaling. Monocyte stimulation by NDPKs also resulted in activation of NF-κB and IRF pathways but did not include the formation of pyroptosomes or result in pyroptotic cell death which are pivotal features of canonical NLRP3 inflammasome activation. In the context of the growing importance of the NLRP3 inflammasome and IL-1β in AML and MDS, our findings now implicate pathogen NDPKs in the pathogenesis of these diseases.
العلاقة: https://dx.doi.org/10.1371/journal.pone.0288162Test; Trova S, Lin F, Lomada S, Fenton M, Chauhan B, Adams A, et al. Pathogen and human NDPK-proteins promote AML cell survival via monocyte NLRP3-inflammasome activation. PloS one. 2023;18(7):e0288162. PubMed PMID: 37418424. Pubmed Central PMCID: PMC10328239. Epub 2023/07/07. eng.; http://hdl.handle.net/10541/626445Test; PLoS One
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9دورية أكاديمية
المؤلفون: El-Battrawy, I., Müller, J., Zhao, Z., Cyganek, L., Zhong, R., Zhang, F., Kleinsorge, M., Lan, H., Li, X., Xu, Q., Huang, M., Liao, Z., Moscu-Gregor, A., Albers, S., Dinkel, H., Lang, S., Diecke, S., Zimmermann, W.H., Utikal, J., Wieland, T., Borggrefe, M., Zhou, X., Akin, I.
مصطلحات موضوعية: Technology Platforms
الوصف: BACKGROUND: Among rare channelopathies BrS patients are at high risk of sudden cardiac death (SCD). SCN5A mutations are found in a quarter of patients. Other rare gene mutations including SCN1B have been implicated to BrS. Studying the human cellular phenotype of BrS associated with rare gene mutation remains lacking. OBJECTIVES: We sought to study the cellular phenotype of BrS with the SCN1B gene variants using human-induced pluripotent stem cell (hiPSCs)–derived cardiomyocytes (hiPSC-CMs). METHODS AND RESULTS: A BrS patient suffering from recurrent syncope harboring a two variants (c.629T > C and c.637C > A) in SCN1B, which encodes the function-modifying sodium channel beta1 subunit, and three independent healthy subjects were recruited and their skin biopsies were used to generate hiPSCs, which were differentiated into cardiomyocytes (hiPSC-CMs) for studying the cellular electrophysiology. A significantly reduced peak and late sodium channel current (I(Na)) and a shift of activation curve to more positive potential as well as a shift of inactivation curve to more negative potential were detected in hiPSC-CMs of the BrS patient, indicating that the SCN1B variants impact the function of sodium channels in cardiomyocytes. The reduced I(Na) led to a reduction of amplitude (APA) and upstroke velocity (V(max)) of action potentials. Ajmaline, a sodium channel blocker, showed a stronger effect on APA and Vmax in BrS cells as compared to cells from healthy donors. Furthermore, carbachol was able to increase arrhythmia events and the beating frequency in BrS. CONCLUSION: Our hiPSC-CMs from a BrS-patient with two variants in SCN1B recapitulated some key phenotypic features of BrS and can provide a platform for studies on BrS with SCN1B variants.
وصف الملف: application/pdf; application/msword
العلاقة: http://edoc.mdc-berlin.de/18532/1/18532oa.pdfTest; http://edoc.mdc-berlin.de/18532/8/18532suppl.docTest; Studying Brugada syndrome with an SCN1B variants in human-induced pluripotent stem cell-derived cardiomyocytes. El-Battrawy, I. and Müller, J. and Zhao, Z. and Cyganek, L. and Zhong, R. and Zhang, F. and Kleinsorge, M. and Lan, H. and Li, X. and Xu, Q. and Huang, M. and Liao, Z. and Moscu-Gregor, A. and Albers, S. and Dinkel, H. and Lang, S. and Diecke, S. and Zimmermann, W.H. and Utikal, J. and Wieland, T. and Borggrefe, M. and Zhou, X. and Akin, I. Frontiers in Cell and Developmental Biology 7 : 261. 1 November 2019
الإتاحة: https://doi.org/10.3389/fcell.2019.00261Test
http://edoc.mdc-berlin.de/18532Test/
https://edoc.mdc-berlin.de/18532Test/
http://edoc.mdc-berlin.de/18532/1/18532oa.pdfTest
http://edoc.mdc-berlin.de/18532/8/18532suppl.docTest -
10دورية أكاديمية
المؤلفون: Eraslan, B., Wang, D., Gusic, M., Prokisch, H., Hallström, B.M., Uhlén, M., Asplund, A., Pontén, F., Wieland, T., Hopf, T., Hahne, H., Kuster, B., Gagneur, J.
المصدر: Mol. Syst. Biol. 15:e8513 (2019)
مصطلحات موضوعية: Codon Usage, Mrna Sequence Motifs, Proteomics, Transcriptomics, Translational Control
الوصف: Despite their importance in determining protein abundance, a comprehensive catalogue of sequence features controlling protein-to-mRNA (PTR) ratios and a quantification of their effects are still lacking. Here, we quantified PTR ratios for 11,575 proteins across 29 human tissues using matched transcriptomes and proteomes. We estimated by regression the contribution of known sequence determinants of protein synthesis and degradation in addition to 45 mRNA and 3 protein sequence motifs that we found by association testing. While PTR ratios span more than 2 orders of magnitude, our integrative model predicts PTR ratios at a median precision of 3.2-fold. A reporter assay provided functional support for two novel UTR motifs, and an immobilized mRNA affinity competition-binding assay identified motif-specific bound proteins for one motif. Moreover, our integrative model led to a new metric of codon optimality that captures the effects of codon frequency on protein synthesis and degradation. Altogether, this study shows that a large fraction of PTR ratio variation in human tissues can be predicted from sequence, and it identifies many new candidate post-transcriptional regulatory elements.
وصف الملف: application/pdf
العلاقة: info:eu-repo/semantics/altIdentifier/pmid/30777893; info:eu-repo/semantics/altIdentifier/wos/WOS:000459628300004; info:eu-repo/semantics/altIdentifier/isbn/1744-4292; info:eu-repo/semantics/altI; https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=55577Test; urn:isbn:1744-4292; urn:issn:1744-4292
الإتاحة: https://doi.org/10.15252/msb.20188513Test
https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=55577Test
النص الكامل