المؤلفون: Gang Wu, Chen Qian, Wen-Li Lv, Xiaona Zhao, Xian-Wei Liu

المصدر: Nature Communications, Vol 14, Iss 1, Pp 1-10 (2023)

مصطلحات موضوعية: Science

الوصف: Abstract Direct visualization of surface chemical dynamics in solution is essential for understanding the mechanisms involved in nanocatalysis and electrochemistry; however, it is challenging to achieve high spatial and temporal resolution. Here, we present an azimuth-modulated plasmonic imaging technique capable of imaging dynamic interfacial changes. The method avoids strong interference from reflected light and consequently eliminates the parabolic-like interferometric patterns in the images, allowing for a 67-fold increase in the spatial resolution of plasmonic imaging. We demonstrate that this optical imaging approach enables comprehensive analyses of surface chemical dynamics and identification of previously unknown surface reaction heterogeneity by investigating electrochemical redox reactions over single silver nanowires as an example. This work provides a general strategy for high-resolution plasmonic imaging of surface electrochemical dynamics and other interfacial chemical reactions, complementing existing surface characterization methods.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2041-1723Test

الوصول الحر: https://doaj.org/article/13b07ba84c1048a98ec8be6dce1435ddTest

المؤلفون: Yi-Nan Liu (524291), Zhen-Ting Lv (10126053), Wen-Li Lv (10867294), Dong-Feng Liu (3945140), Xian-Wei Liu (1793137)

مصطلحات موضوعية: Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Biotechnology, Immunology, Infectious Diseases, Computational Biology, Space Science, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, unveiling ample heterogeneities, electrochemical activity parameters, plasmonic imaging technique, microbial cells using, noninvasive imaging method, free optical imaging, microbial cells, various biological, situ <, remains challenging, redox reaction, formal potential, electrons transferred, electron transfer, directly probe, chemical processes, cell level, c <

الوصف: Measurement of electron transfer at the single-particle or -cell level is crucial to the in situ study of basic chemical and biological processes. However, it remains challenging to directly probe the microbial extracellular electron transfer process due to the weakness of signals and the lack of techniques. Here, we present a label-free and noninvasive imaging method that is able to measure the electron transfer in microbial cells. We measured the extracellular electron transfer processes by imaging the redox reaction of c -type outer membrane cytochromes in microbial cells using a plasmonic imaging technique, and obtained the electrochemical activity parameters (formal potential and number of electrons transferred) of multiple individual microbial cells, allowing for unveiling ample heterogeneities in electron transfer at the single-cell level. We anticipate that this method will contribute to the study of electron transfer in various biological and chemical processes.

العلاقة: https://figshare.com/articles/journal_contribution/Label-Free_Optical_Imaging_of_the_Electron_Transfer_in_Single_Live_Microbial_Cells/21806642Test

الإتاحة: https://doi.org/10.1021/acs.nanolett.2c04018.s001Test

المؤلفون: Gang Wu, Xiaoli Zhou, Wen-Li Lv, Chen Qian, Xian-Wei Liu

المصدر: Nano Letters. 22:4383-4391

مصطلحات موضوعية: Microscopy, Interferometry, Mechanical Engineering, Optical Imaging, Nanoparticles, Nanotechnology, General Materials Science, Bioengineering, General Chemistry, Condensed Matter Physics

الوصف: Real-time probing of the compositional evolution of single nanoparticles during an electrochemical reaction is crucial for understanding the structure-performance relationship and rationally designing nanomaterials for desirable applications; however, it is consistently challenging to achieve high-throughput real-time tracking. Here, we present an optical imaging method, termed plasmonic scattering interferometry microscopy (PSIM), which is capable of imaging the compositional evolution of single nanoparticles during an aqueous electrochemical reaction in real time. By quantifying the plasmonic scattering interferometric pattern of nanoparticles, we establish the relationship between the pattern and composition of single nanoparticles. Using PSIM, we have successfully probed the compositional transformation dynamics of multiple individual nanoparticles during electrochemical reactions. PSIM could be used as a universal platform for exploring the compositional evolution of nanomaterials at the single-nanoparticle level and offers great potentials for addressing the extensive fundamental questions in nanoscience and nanotechnology.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ee93c7d283cb8d104e0bece36f3516eTest
https://doi.org/10.1021/acs.nanolett.2c00831Test

المؤلفون: Yi-Nan Liu, Zhen-Ting Lv, Wen-Li Lv, Dong-Feng Liu, Xian-Wei Liu

المصدر: Nano letters.

مصطلحات موضوعية: Mechanical Engineering, General Materials Science, Bioengineering, General Chemistry, Condensed Matter Physics

الوصف: Measurement of electron transfer at the single-particle or -cell level is crucial to the

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae4f113413578ca9d2632eb3136beb23Test
https://pubmed.ncbi.nlm.nih.gov/36594792Test

المؤلفون: Jie Li, Kai Zhang, Xi Zhang, Wen-li Lv, Yin-yin Gou, Min-kang Zhi, Jin-juan Li

المصدر: Polish Journal of Environmental Studies. 31:735-747

مصطلحات موضوعية: Environmental Chemistry, General Environmental Science

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::c8ec07318bc51d4f840d3d1a60fc7904Test
https://doi.org/10.15244/pjoes/140560Test

المؤلفون: Xian-Wei Liu, Zhen-Ting Lv, Wen-Li Lv, Yi-Nan Liu

المصدر: Proceedings of the National Academy of Sciences of the United States of America

مصطلحات موضوعية: Materials science, Surface Properties, microbial adhesion, Nanotechnology, Biosensing Techniques, 02 engineering and technology, Microscopy, Atomic Force, 010402 general chemistry, 01 natural sciences, Bacterial Adhesion, Biophysical Phenomena, Physical Phenomena, Adhesion strength, plasmonic imaging, Plasmon, interfacial force, Multidisciplinary, Biological entity, Biofilm, Surface Plasmon Resonance, 021001 nanoscience & nanotechnology, Pathogenicity, 0104 chemical sciences, Chemistry, Biofilms, Physical Sciences, biofilm formation, Imaging technique, Single-Cell Analysis, 0210 nano-technology, Biosensor

الوصف: Significance Studying interfacial dynamics of a single biological entity (cell, virus, or organelle) is critical for understanding microbial biofilm formation processes, developing biosensors, and designing biomaterials. However, the ability to measure the binding force between a single biological entity and surface remains a great challenge due to the hydrated feature of biological entity. In this paper, we present an optical imaging method that is able to measure the adhesion strength of single microbial cells. Unlike the existing methods with limited throughput, this method determines the adhesion strength of multiple individual cells simultaneously. This approach has the potential to contribute to a better understanding of the adhesion process at the microscopic scale and probe the biointerfaces.
Probing the binding between a microbe and surface is critical for understanding biofilm formation processes, developing biosensors, and designing biomaterials, but it remains a challenge. Here, we demonstrate a method to measure the interfacial forces of bacteria attached to the surface. We tracked the intrinsic fluctuations of individual bacterial cells using an interferometric plasmonic imaging technique. Unlike the existing methods, this approach determined the potential energy profile and quantified the adhesion strength of single cells by analyzing the fluctuations. This method provides insights into biofilm formation and can also serve as a promising platform for investigating biological entity/surface interactions, such as pathogenicity, microbial cell capture and detection, and antimicrobial interface screening.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47fd469e3cd3779acc2960d23002fd33Test
https://doi.org/10.1073/pnas.2010136117Test

المؤلفون: Xiaona Zhao, Wen-Li Lv, Chen Qian, Hai-Bo Chen, Xian-Wei Liu, Yi-Nan Liu, Di Jiang

المصدر: Analytical chemistry. 93(22)

مصطلحات موضوعية: Diagnostic Imaging, Scattering, Chemistry, Metal ions in aqueous solution, 010401 analytical chemistry, Proteins, 010402 general chemistry, Tracking (particle physics), 01 natural sciences, Small molecule, Receptor–ligand kinetics, Biophysical Phenomena, 0104 chemical sciences, Analytical Chemistry, Interferometry, Kinetics, Chemical physics, Molecule, Particle, Protein Binding

الوصف: Probing molecular interactions is critical for screening drugs, detecting pollutants, and understanding biological processes at the molecular level, but these interactions are difficult to detect, especially for small molecules. A label-free optical imaging technology that can detect molecule binding kinetics is presented, in which free-moving particles are driven into oscillations with an alternating electrical field and the interferometric scattering patterns of the particles are imaged via an optical imaging method. By tracking the charge-sensitive variations in the oscillation amplitude with sub-nanometer precision, the small molecules and metal ions binding to the surface as well as protein-protein binding kinetics were measured. The capability of the label-free measurement of molecular interactions can provide a promising platform for screening small-molecule drugs, probing conformational changes in proteins, and detecting environmental pollutants.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce542cd9cd5257abb1581d3ac7556d9aTest
https://pubmed.ncbi.nlm.nih.gov/34029055Test

المؤلفون: Yi-Nan Liu, Zhen-Ting Lv, Wen-Li Lv, Xian-Wei Liu

المصدر: Proceedings of the National Academy of Sciences of the United States of America; 11/3/2020, Vol. 117 Issue 44, p1-6, 6p

مصطلحات موضوعية: MICROBIAL cells, BACTERIAL cells, ADHESION, SURFACE interactions, MICROBIAL adhesion

مستخلص: Probing the binding between a microbe and surface is critical for understanding biofilm formation processes, developing biosensors, and designing biomaterials, but it remains a challenge. Here, we demonstrate a method to measure the interfacial forces of bacteria attached to the surface. We tracked the intrinsic fluctuations of individual bacterial cells using an interferometric plasmonic imaging technique. Unlike the existing methods, this approach determined the potential energy profile and quantified the adhesion strength of single cells by analyzing the fluctuations. This method provides insights into biofilm formation and can also serve as a promising platform for investigating biological entity/surface interactions, such as pathogenicity, microbial cell capture and detection, and antimicrobial interface screening. [ABSTRACT FROM AUTHOR]

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المؤلفون: Yong Feng Yan, Jun Li, Wen Li Lv, Shuai Gao

المصدر: Advanced Materials Research. 868:3-6

مصطلحات موضوعية: Titanomagnetite, Engineering, Mining engineering, business.industry, General Engineering, Block model, Mineralogy, Jian, business, Digitization

الوصف: From the 21st century, computers are widely used in various fields, in the mines, driven by digitization, the birth of 3Dmine make digital mine entered a new phase, Lanjiahuoshan and Jianbaobao are very important in Panzhihua mine. Based on 3Dmine’s advantages, combined with the geological conditions of two mines, this paper established the underlying database, surface models, physical models, block model. The study confirms 3Dmine digital applications in the mine and provide the basis for the mine design, measurement, the production and management.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::053ab99fa0975f4c2854b5c845491cceTest
https://doi.org/10.4028/www.scientific.net/amr.868.3Test

المؤلفون: Jia Shi Wang, Wen Li Lv, Zai Ke Li, Qi Bin Jiang

المصدر: Advanced Materials Research. 744:383-387

مصطلحات موضوعية: Load capacity, Engineering, business.industry, Leaf spring, Spring (device), Composite number, General Engineering, Trailer truck, Structural engineering, business, Finite element method

الوصف: The composite leaf spring for trailer truck is developed with weight reduction 60% relative to steel spring, which can be benefit to environment protection and energy saving. In this work, the composite leaf spring is designed and analyzed by finite element method. Then the experimental tests are conducted on the composite leaf spring fabricated by the hot molding process. The spring rate and the maximum load capacity are measured, which have a good agreement with the design results.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::61e10949eb26d6294697ad9098c3ade8Test
https://doi.org/10.4028/www.scientific.net/amr.744.383Test