المؤلفون: Yongbin Li, Siyu Chen, Weihong Liu, Di Zhao, Yimeng Gao, Shipeng Hu, Hanyu Liu, Yuanyuan Li, Lei Qu, Xiao Liu

المصدر: Nature Communications, Vol 15, Iss 1, Pp 1-19 (2024)

مصطلحات موضوعية: Science

الوصف: Abstract Invariant cell lineage in C. elegans enables spatiotemporal resolution of transcriptional regulatory mechanisms controlling the fate of each cell. Here, we develop RAPCAT (Robust-point-matching- And Piecewise-affine-based Cell Annotation Tool) to automate cell identity assignment in three-dimensional image stacks of L1 larvae and profile reporter expression of 620 transcription factors in every cell. Transcription factor profile-based clustering analysis defines 80 cell types distinct from conventional phenotypic cell types and identifies three general phenotypic modalities related to these classifications. First, transcription factors are broadly downregulated in quiescent stage Hermaphrodite Specific Neurons, suggesting stage- and cell type-specific variation in transcriptome size. Second, transcription factor expression is more closely associated with morphology than other phenotypic modalities in different pre- and post-differentiation developmental stages. Finally, embryonic cell lineages can be associated with specific transcription factor expression patterns and functions that persist throughout postembryonic life. This study presents a comprehensive transcription factor atlas for investigation of intra-cell type heterogeneity.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2041-1723Test

الوصول الحر: https://doaj.org/article/25314374eba145dab951a0d903db0102Test

المؤلفون: Weihong Liu, Qiangyu Ren, Jiaojiao Deng, Jincheng Guo, Xiaoming Zheng, Hongli Liu

المصدر: IET Energy Systems Integration, Vol 5, Iss 4, Pp 418-429 (2023)

مصطلحات موضوعية: battery storage plants, power markets, renewable energy sources, wind power plants, Production of electric energy or power. Powerplants. Central stations, TK1001-1841, Energy industries. Energy policy. Fuel trade, HD9502-9502.5

الوصف: Abstract The fluctuation and stochastic characteristics of renewable energy resources challenge the secure system operation and also impose significant financial risks for the market participating renewable energy plants (REPs). Energy storage systems (ESSs) can serve as effective tools in enhancing the operating flexibility of REPs, thus improving their profitability while making them grid‐friendly. However, current studies focussing on the energy market participation of ESS‐equipped REPs neglect ESSs' frequency regulation performance. Moreover, power output deviation and power curtailment of REPs bring difficulties to the integration of renewable energy. To address these challenges, an optimal ESS configuration method for REPs participating in the joint energy‐regulation market is proposed first. A method considering constraints on frequency regulation performance is applied. Then, to reduce power output deviation and power curtailment of REPs, an incentive mechanism based on the excess revenue recovery is implemented to induce the grid‐friendly power outputs of REPs. Numerical analysis on XJTU‐ROTS2017 systems demonstrates that the optimal ESS configuration results obtained from the proposed method could promote the profitability of ESS‐equipped REPs. The results also verify the effectiveness of the excess revenue recovery mechanism in facilitating the grid‐friendly operation behaviours of the ESS‐equipped REPs.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2516-8401Test

الوصول الحر: https://doaj.org/article/f83ab45dba5148d69c9efda10bf28f3cTest

المؤلفون: Yunsong Zhao, Na Li, Li Wang, Junyang He, Weihong Liu, Min Song

المصدر: Materials Research Letters, Vol 11, Iss 10, Pp 888-895 (2023)

مصطلحات موضوعية: Ni-based superalloy, Ru-bearing, site occupation, ordering, creep rupture, Materials of engineering and construction. Mechanics of materials, TA401-492

الوصف: By using state-of-the-art characterisation techniques including atom probe tomography and atomic resolved elemental mapping, we successfully probed the site occupation evolution associated with composition change in γ′, during 1100°C creep of a fourth generation Ru-bearing Ni-based superalloy. It is quantified that, W and Ru maintain unchanged site preference after creep rupture, while interestingly, the rest elements especially Co, Ta and Re show a weakened preference at both α- and β-sites in the γ′ lattice. This indicates the overall reduced γ′ ordering degree and thus the possible decrease in planar fault energies, which further facilitates dislocation shearing in γ′.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2166-3831Test

الوصول الحر: https://doaj.org/article/4460c21924e14f83891da19c1c4398abTest

المؤلفون: Kexin Xu, Dongdong Tian, TingJin Wang, Aijun Zhang, Mohamed Abdou Youssef Elsadek, Weihong Liu, Liping Chen, Yongfeng Guo

المصدر: Molecular Horticulture, Vol 3, Iss 1, Pp 1-19 (2023)

مصطلحات موضوعية: Small secreted peptide, Tomato, Drought stress, CEP peptide, Plant culture, SB1-1110, Botany, QK1-989

الوصف: Abstract Tomato (Solanum lycopersicum) is one of the most important vegetable crops in the world and abiotic stresses often cause serious problems in tomato production. It is thus important to identify new regulators in stress response and to devise new approaches to promote stress tolerance in tomato. Previous studies have shown that small secreted peptides (SSPs) are important signal molecules regulating plant growth and stress response by mediating intercellular communication. However, little is known about tomato SSPs, especially their roles in responding to abiotic stresses. Here we report the identification of 1,050 putative SSPs in the tomato genome, 557 of which were classified into 38 known SSP families based on their conserved domains. GO and transcriptome analyses revealed that a large proportion of SlSSPs might be involved in abiotic stress response. Further analysis indicated that stress response related cis-elements were present on the SlCEP promotors and a number of SlCEPs were significantly upregulated by drought treatments. Among the drought-inducible SlCEPs, SlCEP10 and SlCEP11b were selected for further analysis via exogenous application of synthetic peptides. The results showed that treatments with both SlCEP10 and SlCEP11b peptides enhanced tomato drought stress tolerance, indicating the potential roles of SlSSPs in abiotic stress response. Graphical Abstract

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2730-9401Test

الوصول الحر: https://doaj.org/article/76a9bc556abc4e2196e0b7fc5291b235Test

المؤلفون: Siyu Chen, Weihong Liu, Lei Xiong, Zhiju Tao, Di Zhao

المصدر: RNA Biology, Vol 21, Iss 1, Pp 1-10 (2024)

مصطلحات موضوعية: C. elegans, transgene silencing, endo-RNAi, tissue-specific, Genetics, QH426-470

الوصف: ABSTRACTTransgene silencing is a common phenomenon observed in Caenorhabditis elegans, particularly in the germline, but the precise mechanisms underlying this process remain elusive. Through an analysis of the transcription factors profile of C. elegans, we discovered that the expression of several transgenic reporter lines exhibited tissue-specific silencing, specifically in the intestine of C. elegans. Notably, this silencing could be reversed in mutants defective in endogenous RNA interference (RNAi). Further investigation using knock-in strains revealed that these intestine-silent genes were indeed expressed in vivo, indicating that the organism itself regulates the intestine-specific silencing. This tissue-specific silencing appears to be mediated through the endo-RNAi pathway, with the main factors of this pathway, mut-2 and mut-16, are significantly enriched in the intestine. Additionally, histone modification factors, such as met-2, are involved in this silencing mechanism. Given the crucial role of the intestine in reproduction alongside the germline, the transgene silencing observed in the intestine reflects the self-protective mechanisms employed by the organisms. In summary, our study proposed that compared to other tissues, the transgenic silencing of intestine is specifically regulated by the endo-RNAi pathway.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1547-6286Test; https://doaj.org/toc/1555-8584Test

الوصول الحر: https://doaj.org/article/c149a1698de14bbba6bd5cd90d2e12aeTest

المؤلفون: Junyang He, Rui Wang, Na Li, Zhongrun Xiao, Ji Gu, Hongyao Yu, Zhongnan Bi, Weihong Liu, Min Song

المصدر: Materials Research Letters, Vol 11, Iss 4, Pp 281-288 (2023)

مصطلحات موضوعية: Ni-based superalloy, additive manufacturing, cracking, elemental segregation, atom probe tomography, Materials of engineering and construction. Mechanics of materials, TA401-492

الوصف: ABSTRACTIn this work, by using multi-scale characterizations from electron channeling contrast imaging (ECCI) to atom probe tomography (APT), we directly evidenced that the massive cracking events in the selective-laser-melted (SLMed) Haynes 230 superalloy are due to the continuous decoration of an M23C6-type thin film at grain boundaries. The high-melting-point nature of the carbide rules out the possibility of liquidation cracking, while the long and straight film surface facilitates stress-induced solid-state cracking. Impurities, Si, Mn and Fe, greatly enhance the cracking susceptibility despite the interesting fact that they are strongly depleted from the carbide.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2166-3831Test

الوصول الحر: https://doaj.org/article/c0bce756a56f4fea9f43ba380bb3668bTest

المؤلفون: Yuqi Wen, Yang Liu, Weihong Liu, Wenli Liu, Jinyan Dong, Qingkuo Liu, Hao Hao, Hongsheng Ren

المصدر: Immunity, Inflammation and Disease, Vol 11, Iss 10, Pp n/a-n/a (2023)

مصطلحات موضوعية: activation mechanism, cytokines, inflammatory response, nucleotide‐binding oligomerized structural domain‐like receptor protein 3 inflammasome, septic cardiomyopathy, Immunologic diseases. Allergy, RC581-607

الوصف: Abstract Sepsis is an uncontrolled host response to infection, resulting in a clinical syndrome involving multiple organ dysfunctions. Cardiac damage is the most common organ damage in sepsis. Uncontrolled inflammatory response is an important mechanism in the pathogenesis of septic cardiomyopathy (SCM). NLRP3 inflammasome promotes inflammatory response by controlling the activation of caspase‐1 and the release of pro‐inflammatory cytokines interleukin IL‐1β and IL‐18. The role of NLRP3 inflammasome has received increasing attention, but its activation mechanism and regulation of inflammation in SCM remain to be investigated.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2050-4527Test

الوصول الحر: https://doaj.org/article/caeffb74dd074136ba66b37c5de71d2eTest

المؤلفون: Baoqing Zhang, Zhaolin Li, Mengfa Wang, Haotian Ling, Yiming Wang, Xijian Zhang, Weihong Liu, Victor Qi, Kunfeng Chen, Gongbin Tang, Aimin Song, Yifei Zhang

المصدر: IEEE Access, Vol 11, Pp 98305-98314 (2023)

مصطلحات موضوعية: Bandpass filter, miniaturization, narrowband, substrate integrated waveguide (SIW), spoof surface plasmon polariton (SSPP), Electrical engineering. Electronics. Nuclear engineering, TK1-9971

الوصف: In this paper, a narrowband bandpass filter comprising substrate integrated waveguide (SIW) and spoof surface plasmon polaritons (SSPPs) is proposed, achieving miniaturization and low insertion loss at Ka-band. SIWs and SSPPs exhibit low and high cut-off responses, respectively, which can be combined for filtering functionalities. By designing I-shaped SSPP structures with large effective length, the cut-off frequencies of SIW and SSPPs can be tailed to converge at a certain frequency for the narrowband filter, where the SIW mode functions as a quasi-evanescent mode, and the mode impedance ramps fast. A sharp funnel-shaped transition is designed to connect SIW with a large impedance and 50- $\Omega $ microstrip line for low insertion loss and a compact profile. In addition, no transitional SSPP structures are desired in the proposed devices, which further shortens the profile dimension. To verify our design, a hybrid filter at 30 GHz is fabricated and characterized. The measured insertion loss is as low as 1.5 dB, and the 3-dB bandwidth is as narrow as 3%, which shows good consistency with the simulation. The proposed SIW-SSPP device has a low profile of $0.70\,\,\lambda \times 0.38\,\,\lambda $ and may find important applications in frequency division multiplexing.

وصف الملف: electronic resource

العلاقة: https://ieeexplore.ieee.org/document/10239398Test/; https://doaj.org/toc/2169-3536Test

الوصول الحر: https://doaj.org/article/011f2b93a65048838191e92f8a55d954Test

المؤلفون: Zhongtao Wu, Jianjian Song, Mingbiao Xu, Weihong Liu, Rongyao Chen, Lei Pu, Shanshan Zhou

المصدر: Arabian Journal of Chemistry, Vol 16, Iss 5, Pp 104670- (2023)

مصطلحات موضوعية: Oil well cement-based composites, Carbonation, Weighting materials, High temperature, Carbon dioxide, Chemistry, QD1-999

الوصف: As an indispensable part of cement slurry for high temperature and high pressure oil and gas wells, weighting materials have a significant impact on the carbon dioxide corrosion of oil well cement-based composites.This paper studied the carbonation process of cement with three weighting agents, and evaluated the compressive strength and carbonation depth of cement at 150 ℃. XRD, SEM and MIP were used to study the carbonation mechanism of cement. When 21 days of carbonation, the carbonation depth growth rate of hausmannite cement was 0.21 mm/d, hematite cement was 0.24 mm/d, and barite cement was 0.31 mm/d. The compressive strength of cement decreased after carbonation,and the carbonation had a minor influence on the compressive strength of hausmannite cement and the most significant impact on barite cement. The carbonation product of oil well cement was mainly calcite. Unstable vaterite mainly existed in the barite cement sample, indicating that the barite cement sample was the most serious corrosion. In the carbonation zone, the number of pores smaller than 10 nm increased the most in the hausmannite cement sample. Pores with a diameter greater than 100 nm accounted for 1.9 % in the hausmannite cement, 3.0 % in hematite cement, and 4.8 % in barite cement. The result shows that hausmannite is the most conducive to the corrosion resistance of oil well cement.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S1878535223001326Test; https://doaj.org/toc/1878-5352Test

الوصول الحر: https://doaj.org/article/ad01207fdf914186a5b728b100d7b5c9Test

المؤلفون: Yu HUANG, Weihong LIU, Hongyang WANG, Qianer ZHU, Xiaoxiao LANG, Wei WANG, Shenglong CHEN, Hui YU

المصدر: Shipin gongye ke-ji, Vol 43, Iss 13, Pp 180-189 (2022)

مصطلحات موضوعية: undaria pinnatifida sporophyll, bionic enzymolysis, response surface optimization, antioxidant activity, Food processing and manufacture, TP368-456

الوصف: Bioactive peptides were prepared from Undaria pinnatifida sporophyll by bionic enzymatic hydrolysis. Response surface methodology (RSM) was used to optimize the enzymatic hydrolysis process based on single factor test with the polypeptide yield and degree of hydrolysis as the main indexes, and the scavenging ability of polypeptide on three free radicals (DPPH, ABTS, OH) was evaluated. The results showed that the optimal conditions of biomimetic enzymatic hydrolysis were as follows: Ultrasonic power 720 W, ultrasonic wall breaking time 40 min, solid-liquid ratio 1:104 g/mL, pepsin dosage 6.1%, enzymatic hydrolysis time 2.5 h, trypsin dosage 5%, enzymatic hydrolysis time 3 h. Under these conditions, the polypeptide yield was 21.17% and the degree of hydrolysis was 43.07%. Enzymatic peptide had good scavenging effects on DPPH, ABTS and OH radicals with IC50 values of 5.71, 1.31 and 1.35 mg/mL, respectively. The optimized bionic enzymatic hydrolysis process of Undaria pinnatifida sporophyll was reasonable and feasible, and the enzymatic peptide had good free radical scavenging ability, indicating that the enzymatic peptide had good antioxidant activity and could be used as ingredients in the development of functional food.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1002-0306Test

الوصول الحر: https://doaj.org/article/a6c4fb9e27d84fe98a4bda8dd674acc3Test