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1دورية أكاديمية
المؤلفون: Xuan Zhao, Jiaman Pang, Wanghong Zhang, Xie Peng, Zhenguo Yang, Guangdong Bai, Yaoyao Xia
المصدر: Animal Nutrition, Vol 17, Iss , Pp 123-133 (2024)
مصطلحات موضوعية: Tryptophan metabolism, Piglet, Diarrhea, Intestinal microbiota, Inflammatory response, Animal culture, SF1-1100
الوصف: The intestinal architecture of piglets is vulnerable to disruption during weaning transition and leads to diarrhea, frequently accompanied by inflammation and metabolic disturbances (including amino acid metabolism). Tryptophan (Trp) plays an essential role in orchestrating intestinal immune tolerance through its metabolism via the kynurenine, 5-hydroxytryptamine, or indole pathways, which could be dictated by the gut microbiota either directly or indirectly. Emerging evidence suggests a strong association between piglet diarrhea and Trp metabolism. Here we aim to summarize the intricate balance of microbiota–host crosstalk by analyzing alterations in both the host and microbial pathways of Trp and discuss how Trp metabolism may affect piglet diarrhea. Overall, this review could provide valuable insights to explore effective strategies for managing piglet diarrhea and the related challenges.
وصف الملف: electronic resource
العلاقة: http://www.sciencedirect.com/science/article/pii/S2405654524000350Test; https://doaj.org/toc/2405-6545Test
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2دورية أكاديمية
المؤلفون: Liang Xiong, Md. Abul Kalam Azad, Yang Liu, Wanghong Zhang, Qian Zhu, Chengjun Hu, Jinming You, Xiangfeng Kong
المصدر: Antioxidants, Vol 13, Iss 3, p 283 (2024)
مصطلحات موضوعية: colonic microbiota, growing-finishing pigs, intrauterine growth restriction, metabolomics, Therapeutics. Pharmacology, RM1-950
الوصف: Intrauterine growth restriction (IUGR) pigs are characterized by long-term growth failure, metabolic disorders, and intestinal microbiota imbalance. The characteristics of the negative effects of IUGR at different growth stages of pigs are still unclear. Therefore, this study explored through multi-omics analyses whether the IUGR damages the intestinal barrier function and alters the colonization and metabolic profiles of the colonic microbiota in growing-finishing pigs. Seventy-two piglets (36 IUGR and 36 NBW) were allocated for this trial to analyze physiological and plasma biochemical parameters, as well as oxidative damage and inflammatory response in the colon. Moreover, the colonic microbiota communities and metabolome were examined using 16s rRNA sequencing and metabolomics technologies to reveal the intestinal characteristics of IUGR pigs at different growth stages (25, 50, and 100 kg). IUGR altered the concentrations of plasma glucose, total protein, triglycerides, and cholesterol. Colonic tight junction proteins were markedly inhibited by IUGR. IUGR decreased plasma T-AOC, SOD, and GSH levels and colonic SOD-1, SOD-2, and GPX-4 expressions by restraining the Nrf2/Keap1 signaling pathway. Moreover, IUGR increased colonic IL-1β and TNF-α levels while reducing IL-10, possibly through activating the TLR4-NF-κB/ERK pathway. Notably, IUGR pigs had lower colonic Streptococcus abundance and Firmicutes-to-Bacteroidetes ratio at the 25 kg BW stage while having higher Firmicutes abundance at the 100 kg BW stage; moreover, IUGR pigs had lower SCFA concentrations. Metabolomics analysis showed that IUGR increased colonic lipids and lipid-like molecules, organic acids and derivatives, and organoheterocyclic compounds concentrations and enriched three differential metabolic pathways, including linoleic acid, sphingolipid, and purine metabolisms throughout the trial. Collectively, IUGR altered the nutrient metabolism, redox status, and colonic microbiota community and metabolite profiles of pigs and continued to disrupt colonic barrier function by reducing antioxidant capacity via the Nrf2/Keap1 pathway and activating inflammation via the TLR4-NF-κB/ERK pathway during the growing-finishing stage. Moreover, colonic Firmicutes and Streptococcus could be potential regulatory targets for modulating the metabolism and health of IUGR pigs.
وصف الملف: electronic resource
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3دورية أكاديمية
المؤلفون: Yang Liu, Md. Abul Kalam Azad, Wanghong Zhang, Liang Xiong, Francois Blachier, Zugong Yu, Xiangfeng Kong
المصدر: Journal of Animal Science and Biotechnology, Vol 13, Iss 1, Pp 1-18 (2022)
مصطلحات موضوعية: Bile acid metabolism, Glucolipid metabolism, Intestinal microbiota, Intrauterine growth retardation, Pigs, Animal culture, SF1-1100, Veterinary medicine, SF600-1100
الوصف: Abstract Background Intrauterine growth retardation (IUGR) is associated with severely impaired nutrient metabolism and intestinal development of pigs. Our previous study found that IUGR altered intestinal microbiota and metabolites in the colon. However, the consequences of IUGR on bile acid metabolism in pigs remained unclear. The present study aimed to investigate the bile acid metabolism in the liver and the profile of bile acid derivatives in the colon of growing pigs with IUGR using bile acid targeted metabolomics. Furthermore, we determined correlations between colonic microbiota composition and metabolites of IUGR and normal birth weight (NBW) pigs at different growth stages that were 7, 21, and 28-day-old, and the average body weight (BW) of 25, 50, and 100 kg of the NBW pigs. Results The results showed that the plasma total bile acid concentration was higher (P
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/2049-1891Test
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4دورية أكاديمية
المؤلفون: Wu Tang, Wanghong Zhang, Md. Abul Kalam Azad, Cui Ma, Qian Zhu, Xiangfeng Kong
المصدر: Frontiers in Microbiology, Vol 13 (2022)
مصطلحات موضوعية: colonic microbiota, intrauterine growth restriction, metabolism, piglets, short-chain fatty acids, Microbiology, QR1-502
الوصف: Newborn animals with intrauterine growth restriction (IUGR) are characterized by impaired intestinal structure and function; however, their intestinal microbiota and metabolome profiles have not been fully identified. The present study investigated the differences in colonic microbiota, metabolomics, and barrier function-related gene expression profiles between the IUGR and normal birth weight (NBW) piglets at 7, 21, and 28 days of age. Forty-eight piglets (24 NBW and 24 IUGR) from 24 litters were assigned to assess the differences in colonic microbiota, metabolomics, and gene expression between IUGR and NBW piglets. Compared with the NBW piglets, IUGR piglets showed decreased Shannon index and increased Simpson index at 7 days of age and Chao1 index at 21 days of age (p < 0.05). The IUGR piglets had lower abundances of Firmicutes, Subdoligranulum, Ruminococcaceae_UCG-002, and Ruminococcaceae_UCG-003 at 7 days of age, and Bacteroidetes, Phascolarctobacterium, and Ruminococcaceae_UCG-005 at 21 days of age, when compared with the NBW piglets (p < 0.05). Metabolomics analysis showed significant changes in 147 metabolites mainly involved in organic acids and their derivatives in the colon. Six differential metabolic pathways were significantly enriched, including purine metabolism, amino sugar/nucleotide sugar metabolism, ubiquinone/other terpenoid-quinone biosynthesis, phenylalanine/tyrosine/tryptophan biosynthesis, phenylalanine metabolism, and histidine metabolism. Spearman’s correlation analysis further demonstrated significant correlations between colonic microbiota and metabolites. In addition, colonic isobutyrate at 7 days of age, isovalerate and total short-chain fatty acids (SCFAs) at 21 days of age, and acetate, propionate, butyrate, and total SCFAs levels at 28 days of age were lower and isovalerate was higher at 28 days of age in the IUGR piglets than in the NBW piglets (p < 0.05). Furthermore, the mRNA expression of zonula occludens (ZO)-1 at 7 days of age, ZO-1, occludin, and interleukin (IL)-4 at 21 days of age were down-regulated in the IUGR piglets, whereas tumor necrosis factor (TNF)-α and nuclear factor-kappa B (NF-κB) at 28 days of age were up-regulated, when compared with the NBW piglets (p < 0.05). The findings suggest that the IUGR pigs present abnormal microbiota and nutrient metabolism in the colon, which may further affect the intestine barrier function by regulating gene expressions.
وصف الملف: electronic resource
العلاقة: https://www.frontiersin.org/articles/10.3389/fmicb.2022.989060/fullTest; https://doaj.org/toc/1664-302XTest
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5دورية أكاديمية
المؤلفون: Liang Xiong, Jinming You, Wanghong Zhang, Qian Zhu, Francois Blachier, Yulong Yin, Xiangfeng Kong
المصدر: Journal of Animal Science and Biotechnology, Vol 11, Iss 1, Pp 1-18 (2020)
مصطلحات موضوعية: Growing-finishing pigs, Growth performance, Intrauterine growth restriction, Microbial community, Small intestine, Animal culture, SF1-1100, Veterinary medicine, SF600-1100
الوصف: Abstract Background The interaction of the gut microbiota with key metabolic and physiological processes may be associated with poor growth outcomes in animals born with intrauterine growth restriction (IUGR). Results Growth performance, plasma hormone concentrations, and intestinal microbiota composition were analyzed in IUGR pigs and in normal birth weight (NBW) pigs when the NBW pigs reached 25, 50, and 100 kg of body weight (BW). Compared to NBW pigs, IUGR pigs had lower initial, weaned, and final BW, and lower average daily gain and average daily feed intake in all the considered time points. In the 25 kg BW group, IUGR pigs had higher concentrations of plasma ghrelin and pancreatic polypeptide (PP), but lower insulin concentration than NBW pigs, while the situation was reversed in the 50 kg BW group. As compared to NBW pigs, IUGR pigs had higher microbial alpha diversity in the jejunum and ileum; in the 50 and 100 kg BW groups, IUGR pigs had higher Firmicutes abundance but lower Proteobacteria abundance in the jejunum, and lower Lactobacillus abundance in the jejunum and ileum; in the 25 kg BW group, IUGR pigs showed higher unclassified Ruminococcaceae abundance in the ileum; and in 25 and 50 kg BW groups, IUGR pigs showed lower Ochrobactrum abundance in the jejunum. Spearman’s correlation revealed that Lactobacillus was negatively correlated with growth performance, while unclassified Ruminococcaceae was positively correlated. Predictive metagenomic analysis detected significantly different expression of genes in the intestinal microbiota between IUGR and NBW pigs, suggesting different metabolic capabilities between the two groups. Conclusions Growing-finishing IUGR pigs showed lower growth performance, higher microbial alpha diversity, and differences in plasma hormone concentrations compared to NBW pigs. Alterations in the abundance of Firmicutes, Proteobacteria, Ruminococcaceae, Lactobacillus, and Ochrobactrum in the small intestine may be associated with IUGR, and may therefore serve as a future target for gut microbiota intervention in growing-finishing IUGR pigs.
وصف الملف: electronic resource
العلاقة: http://link.springer.com/article/10.1186/s40104-020-00490-xTest; https://doaj.org/toc/2049-1891Test
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6دورية أكاديمية
المؤلفون: Jiayi Su, Wanghong Zhang, Cui Ma, Peifeng Xie, Francois Blachier, Xiangfeng Kong
المصدر: Frontiers in Veterinary Science, Vol 8 (2021)
مصطلحات موضوعية: colon barrier function, intestine morphology, metabolites, microbiota, weaned piglets, xylo-oligosaccharides, Veterinary medicine, SF600-1100
الوصف: The present study determined the effects of dietary xylo-oligosaccharides (XOS) supplementation on the morphology of jejunum and ileum epithelium, fecal microbiota composition, metabolic activity, and expression of genes related to colon barrier function. A total of 150 piglets were randomly assigned to one of five groups: a blank control group (receiving a basal diet), three XOS groups (receiving the basal diet supplemented with 100, 250, and 500 g/t XOS, respectively), as well as a positive control group, used as a matter of comparison, that received the basal diet supplemented with 0.04 kg/t virginiamycin, 0.2 kg/t colistin, and 3,000 mg/kg ZnO. The trial was carried out for 56 days. The results showed that the lowest dose tested (100 g/t XOS) increased (P < 0.05) the ileal villus height, the relative amount of Lactobacillus and Bifidobacterium spp., and the concentration of acetic acid and short-chain fatty acid in feces when compared with the blank control group. In conclusion, dietary 100 g/t XOS supplementation modifies the intestinal ecosystem in weaned piglets in an apparently overall beneficial way.
وصف الملف: electronic resource
العلاقة: https://www.frontiersin.org/articles/10.3389/fvets.2021.680208/fullTest; https://doaj.org/toc/2297-1769Test
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7دورية أكاديمية
المؤلفون: Cui Ma, Wanghong Zhang, Qiankun Gao, Qian Zhu, Mingtong Song, Hao Ding, Yulong Yin, Xiangfeng Kong
المصدر: Journal of Functional Foods, Vol 75, Iss , Pp 104221- (2020)
مصطلحات موضوعية: Fecal microbiota, Metabolites, Reproductive performance, Sows, Synbiotic, Nutrition. Foods and food supply, TX341-641
الوصف: The present study was conducted to evaluate the effects of dietary synbiotic supplementation on reproductive performance, plasma indexes, and fecal microbiota composition and metabolite level in pregnant and lactating sows. Forty-eight pregnant Bama mini-pigs with 3–7 parities were randomly allocated to a control group (a basal diet), an antibiotic group (50 g/t virginiamycin), or a synbiotic group (200 mL/d·head fermentation broth and 500 g/t xylo-oligosaccharides). Compared with the control group, dietary synbiotic supplementation increased the piglet survival rate and decreased colostrum somatic cell numbers, while synbiotic or antibiotic supplementation improved plasma lipid metabolism. The synbiotic supplementation increased fecal Simpson and Shannon indices, and the relative abundances of Coprococcus and Ruminococcus on day 105 of pregnancy compared with the other two groups. Spearman correlation analysis showed a positive correlation between Ruminococcus abundance and bioamine level but a negative correlation between Desulfovibrio abundance and butyrate level. These findings suggested that dietary synbiotic supplementation can improve piglet’s survival and lipid metabolism by altering gut microbiota diversity and composition in pregnant and lactating sows.
وصف الملف: electronic resource
العلاقة: http://www.sciencedirect.com/science/article/pii/S175646462030445XTest; https://doaj.org/toc/1756-4646Test
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8دورية أكاديمية
المؤلفون: Cui Ma, Qiankun Gao, Wanghong Zhang, Qian Zhu, Wu Tang, Francois Blachier, Hao Ding, Xiangfeng Kong
المصدر: Frontiers in Veterinary Science, Vol 7 (2020)
مصطلحات موضوعية: biochemical parameters, gut microbiota, metabolites, sows, suckling piglets, synbiotic, Veterinary medicine, SF600-1100
الوصف: Nutrients in the maternal diet favor the growth and development of suckling piglets and alter their gut microbiota composition and metabolic activity, thus affecting the hosts. The present study analyzed, in suckling piglets from sows receiving antibiotic or synbiotic supplements from pregnancy to lactation, several biochemical parameters, oxidative/anti-oxidative indices, inflammatory cytokines, and ingestion-related factor levels in plasma, as well as colonic microbiota composition and metabolic activity, and mucosal expression of genes related to the intestinal barrier function. Compared with the control group, maternal synbiotic supplementation decreased (P < 0.05) the plasma levels of glucose, AMM, TC, low-density lipoprotein-cholesterol (LDL-C), MDA, H2O2, ghrelin, CCK, PP, IL-1β, IL-2, IL-6, TNF-α, Ala, Cys, Tau, and β-AiBA, the levels of propionate and total short-chain fatty acids (SCFAs) in the colonic luminal content, and colonic abundances of RFN20, Anaerostipes, and Butyricimonas; while increased (P < 0.05) the plasma levels of urea nitrogen (UN), Ile, Leu, α-AAA, α-ABA, and 1-Mehis, as well as colonic abundances of Sphingomonas, Anaerovorax, Sharpea, and Butyricicoccus. Compared with the antibiotic group, maternal synbiotic supplementation decreased (P < 0.05) the plasma levels of glucose, gastrin, and Ala, as well as abundances of Pasteurella and RFN20 and propionate level in the colonic content. Expression of genes coding for E-cadherin, Occludin, ZO-1, ZO-2, IL-10, and interferon-α were down-regulated in the colonic mucosa. The synbiotic supplementation increased (P < 0.05) the plasma levels of UN, Leu, α-ABA, and 1-Mehis, the abundances of Anaerovorax, Sharpea, and Butyricicoccus and expression of genes coding for E-cadherin, Occludin, ZO-1, ZO-2, IL-10, and interferon-α. Spearman correlation analysis showed that there was a positive correlation between colonic Anaerostipes abundance and acetate and SCFAs levels; whereas a negative correlation between Fusobacteria and Fusobacterium abundances and acetate level. These findings suggest that synbiotic supplementation in the maternal diet improved nutrient metabolism and intestinal barrier permeability, reduced oxidative stress, and modified colonic microbiota composition and metabolic activity in suckling piglets.
وصف الملف: electronic resource
العلاقة: https://www.frontiersin.org/articles/10.3389/fvets.2020.575685/fullTest; https://doaj.org/toc/2297-1769Test
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9دورية أكاديمية
المؤلفون: Cui Ma, QianKun Gao, WangHong Zhang, Md. Abul Kalam Azad, XiangFeng Kong
المصدر: Mediators of Inflammation, Vol 2020 (2020)
الوصف: This study was conducted to analyze plasma reproductive hormone and biochemical parameter changes, as well as fecal microbiota composition and metabolites in sows, at different pregnancy and lactation stages, using Bama mini pig as an experimental animal model. We found that plasma prolactin (PRL), progesterone, follicle-stimulating hormone (FSH), and estrogen levels decreased from day 45 to day 105 of pregnancy. Plasma total protein and albumin levels were lower in pregnant sows, while glucose, urea nitrogen, total cholesterol, and high-density lipoprotein-cholesterol, as well as fecal acetate, butyrate, valerate, total short-chain fatty acids, skatole, and tyramine levels, were higher in lactating sows. Interestingly, the lactating sows showed lower α-diversity and Spirochaetes and Verrucomicrobia relative abundances, while pregnant sows showed a higher Proteobacteria relative abundance. Notably, the Akkermansia relative abundance was highest on day 7 of lactation. Spearman analysis showed a positive correlation between plasma triglyceride and cholinesterase levels and Akkermansia and Streptococcus relative abundances. Moreover, Oscillospira and Desulfovibrio relative abundances were also positively correlated with plasma FSH, LH, and E2 levels, as well as PRL and LH with Bacteroides. Collectively, plasma reproductive hormones, biochemical parameters, and fecal microbiota composition and metabolite levels could alter along with pregnancy and lactation, which might contribute to the growth and development demands of fetuses and newborns.
وصف الملف: electronic resource
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10دورية أكاديمية
المؤلفون: Bo Huang, Yong Qin, Wanghong Zhang, Gang Wang
المصدر: Energy Exploration & Exploitation, Vol 36 (2018)
مصطلحات موضوعية: Production of electric energy or power. Powerplants. Central stations, TK1001-1841, Renewable energy sources, TJ807-830
الوصف: Five boreholes were selected in the Gujiao block, Xishan, Taiyuan, China, as calibration wells to identify the coal structure. The geophysical-log responses of the coal structure in the No. 8 coal reservoir, Gujiao block, were investigated using coal-core logging combined with actual observations in the borehole profile of the coal mine. Natural gamma, density, apparent resistivity, and sonic travel time logs were extracted at 0.15 m intervals from 41 undeformed coal, 29 cataclastic coal, and 48 granulated coal regions. Box plots and Fisher’s maximum separation criterion were used to screen and identify sensitive log responses of the coal structure. The coal structure was identified in 31 boreholes using the available logs in the block, and the layered distribution patterns of the coal structure were discussed. The fracturability of the coal structure was divided into types using cluster analysis based on the thickness ratio of the coal structure and the relationship between the coal structure and its permeability. The results show that sensitive log responses for identifying undeformed coal and cataclastic coal are natural gamma, followed by density and apparent resistivity; log responses for identifying cataclastic coal and granulated coal-mylonitized coal are sonic travel time, apparent resistivity and natural gamma. The sensitive log response data were integrated and coal structure identification models were constructed based on the principle of amplifying the log responses to identify the coal structure in the No. 8 coal reservoir. The reservoir generally contains two or three dirt bands, and the coal structure is divided into several independent layers, with the cataclastic coal and granulated coal-mylonitized coal distributed in the middle of the reservoir. The coal structure was classified into four types and four subtypes through cluster analysis of the boreholes. Under the control of the Malan syncline, type I and type II are developed in the No. 8 coal reservoir in the southern part of the study area and in the east and north wings of the Malan syncline; they have good fracturability. Type III and type IV are mainly present in the No. 8 coal reservoir at the synclinal axis; they have poor fracturability. For type IV dominated by granulated coal, it is difficult to improve the reservoir permeability by fracturing; therefore, other strengthened permeability-improving measures should be considered.
وصف الملف: electronic resource
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