المؤلفون: Di Stadio A., Brenner M. J., De Luca P., Albanese M., D'ascanio L., Ralli M., Roccamatisi D., Cingolani C., Vitelli F., Camaioni A., Di Girolamo S., Bernitsas E.

المساهمون: Di Stadio, A., Brenner, M. J., De Luca, P., Albanese, M., D'Ascanio, L., Ralli, M., Roccamatisi, D., Cingolani, C., Vitelli, F., Camaioni, A., Di Girolamo, S., Bernitsas, E.

مصطلحات موضوعية: brain fog, cognitive deficit, COVID-19, headache, mental clouding, neuroinflammation, olfaction, olfactory dysfunction, SARS-CoV-2, smell

الوصف: Smell alteration and cognitive impairment are common features of the Long-COVID Syndrome. Mental clouding, often described as brain fog, might affect smell by altering recollection of odors or through a share mechanism of neuroinflammation. We investigated mental clouding, headache, and cognitive function in adult patients with persistent COVID-19 olfactory dysfunction. This multi-center cross-sectional study enrolled 152 adults with self-reported olfactory dysfunction from 3 tertiary centers specialized in COVID-19 olfactory disorders. Inclusion criteria were smell alterations after COVID-19 persisting over 6 months from infection, age >18 and < 65. Exclusion criteria included smell alterations, headache, or memory problems prior to COVID-19 infection. The patients were evaluated by olfactometry, nasal endoscopy, headache scale, cognitive assessment, Mini Mental State Examination (MMSE), and self-reported measures. Smell dysfunction was stratified and classified based on olfactory deficit severity and presence of olfactory distortion (parosmia, cacosmia). Data on smell disorder, mental clouding, MMSE, and headache were analyzed to assess correlations. Among the 152 patients studied, 50 (32.8%) presented with anosmia, 25 (16.4%) with hyposmia, 10 (6.6%) with parosmia/cacosmia, and 58 patients (38.2%) with a combination of hyposmia and parosmia; seven (4.6%) patients suffered from headache exclusively, and two (1.4%) had headache and mental clouding as their primary symptom. Headache was reported by 76 (50%) patients, and mental clouding by 71 (46.7%). The patients reporting headache, mental clouding, or both, had significantly increased risk of suffering from anosmia and/or hyposmia when compared with their counterparts without these neurological symptoms. No patients had reduced MMSE scores. In our cohort of adult patients with post-COVID-19, smell alterations persisting over 6 months, cognitive impairment and headache were associated with more severe olfactory loss, consistent with neuroinflammatory ...

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/35203918; info:eu-repo/semantics/altIdentifier/wos/WOS:000778576700001; volume:12; issue:2; journal:BRAIN SCIENCES; http://hdl.handle.net/11573/1635971Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85124153739

الإتاحة: https://doi.org/10.3390/brainsci12020154Test
http://hdl.handle.net/11573/1635971Test

المؤلفون: Ferrari G., Rabito S., Gatti L., Ntep N. N., Vitelli F. D., Marchioni M., Rocco B. M., Micali S., Ferrari R., Cindolo L.

المساهمون: Ferrari, G., Rabito, S., Gatti, L., Ntep, N. N., Vitelli, F. D., Marchioni, M., Rocco, B. M., Micali, S., Ferrari, R., Cindolo, L.

مصطلحات موضوعية: Endoscopy, Laser, Prostate, Human, Male, Postoperative Complication, Treatment Outcome, Prostatic Hyperplasia, Transurethral Resection of Prostate

الوصف: BACKGROUND: Green Light laser enucleation of the prostate (GreenLEP) is an endoscopic treatment to treat bladder outlet obstruction in men with large prostate (>100 cc). Herein, we describe our GreenLEP series and describe its safety and efficacy. METHODS: Between February 2014 and April 2019, 120 patients from a single center underwent en-bloc GreenLEP with early apical release. All procedures were performed with the AMS XPS laser generator (set: 120 W for vaporization and 20 W for coagulation). Morcellation was carried out with the Wolf Piranha morcellator. Data concerning the pre-, intra- and postoperative outcomes were prospectively collected. The follow-up data at 6, 12 months and at the last control were collected. RESULTS: The median age was 66.0 (IQR: 61.0-71.0) years; 37.5% of the patients were under antiplatelet/anticoagulant therapy, 15.0% had indwelling catheter history. The median prostate volume and the baseline PSA value were 98.5 mL (IQR 83.0-130.0) and 4.2 ng/mL (IQR: 3.2-6.8), respectively. The median operative and lasing time were 65.0 (IQR: 51.0-83.5) and 6.0 (IQR: 6.0-10.0) minutes, respectively. In the postoperative period 1 patient was transfused. The median follow-up was 18.0 (IQR: 12.0-39.5) months. All patients had significant improvement in terms of improvement of uroflowmetry (median from 9 mL/sec [IQR 7.8, 11.0] to 20.0 [IQR 18.0, 22.0], P<0.001) and symptoms control (IPSS median score from 26.0 mL/sec [IQR 22.0, 28.0] to 7.0 [IQR 6.0, 8.0], P<0.001]) over time. After 12 months 1 patient complained of stress incontinence (1 pad/day) and 1 of "de novo"wet urgency. CONCLUSIONS: En-bloc GreenLEP with early apical release is a safe and effective procedure even for large volume prostates. It allows us to limit the use of laser energy and shorten the operating times with stable and satisfactory longterm outcomes.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/33781016; info:eu-repo/semantics/altIdentifier/wos/WOS:000827248600010; volume:74; issue:4; firstpage:467; lastpage:474; journal:MINERVA UROLOGY AND NEPHROLOGY; http://hdl.handle.net/11380/1286929Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85134452277

الإتاحة: https://doi.org/10.23736/S2724-6051.21.04145-XTest
http://hdl.handle.net/11380/1286929Test

المؤلفون: Di Stadio A., D'ascanio L., Vaira L. A., Cantone E., De Luca P., Cingolani C., Motta G., De Riu G., Vitelli F., Spriano G., De Vincentiis M., Camaioni A., La Mantia I., Ferreli F., Brenner M. J.

المساهمون: Di Stadio, A., D'Ascanio, L., Vaira, L. A., Cantone, E., De Luca, P., Cingolani, C., Motta, G., De Riu, G., Vitelli, F., Spriano, G., De Vincentiis, M., Camaioni, A., La Mantia, I., Ferreli, F., Brenner, M. J.

مصطلحات موضوعية: anosmia, anti-inflammatory, coronaviru, COVID-19, hyposmia, olfaction, olfactory dysfunction, olfactory training, SARS-CoV-2

الوصف: Background: Olfactory training is the only evidence-based treatment for post-viral olfactory dysfunction. Smell disorders after SARS-CoV-2 infection have been attributed to neuroinflammatory events within the olfactory bulb and the central nervous system. Therefore, targeting neuroinflammation is one potential strategy for promoting recovery from post-COVID-19 chronic olfactory dysfunction. Palmitoylethanolamide and luteolin (PEA-LUT) are candidate antiinflammatory/ neuroprotective agents. Objective: To investigate recovery of olfactory function in patients treated with PEA-LUT oral supplements plus olfactory training versus olfactory training plus placebo. Methods: Multicenter double-blinded randomized placebo-controlled clinical trial was held. Eligible subjects had prior COVID-19 and persistent olfactory impairment >6 months after follow-up SARS-CoV-2 negative testing, without prior history of olfactory dysfunction or other sinonasal disorders. Participants were randomized to daily oral supplementation with ultramicronized PEA-LUT 770 mg plus olfactory training (intervention group) or olfactory training with placebo (control). Sniffin’ Sticks assessments were used to test the patients at baseline and 90 days. Results: A total of 185 patients, including intervention (130) and control (55) were enrolled. The intervention group showed significantly greater improvement in olfactory threshold, discrimination, and identification scores compared to controls (p=0.0001). Overall, 92% of patients in the intervention group improved versus 42% of controls. Magnitude of recovery was significantly greater in the intervention group versus control (12.8 + 8.2 versus mean 3.2 + 3), with >10-fold higher prevalence of anosmia in control versus intervention groups at the 90-day endpoint. Conclusion: Among individuals with olfactory dysfunction post-COVID-19, combining PEA-LUT with olfactory training resulted in greater recovery of smell than olfactory training alone. Clinical Trial Registration: (Italian; ...

العلاقة: info:eu-repo/semantics/altIdentifier/wos/WOS:000858546800019; volume:20; issue:10; firstpage:2001; lastpage:2012; numberofpages:12; journal:CURRENT NEUROPHARMACOLOGY; https://hdl.handle.net/11388/313049Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85130994694

الإتاحة: https://doi.org/10.2174/1570159X20666220420113513Test
https://hdl.handle.net/11388/313049Test

المؤلفون: Zhang, Y. (Yongxin), Farhangfar, F. (Farhang), Giblin, L. (Leonard), Wolf, D. (David), Vitelli, F. (Francesca), Zhou, Y. (Yu), Rudd, D. (Donnie)

المصدر: World Journal of Research and Review

الوصف: Hematopoietic stem cells (HSC) are defined by the International Society of Hematotherapy and Graft Engineering (ISHAGE) as those at low side scatter, positive for CD34 and CD45dim for their numeration with flow cytometry. However, we found that these CD34+ cells increase their granularity and size following in vitro culture, which was exhibited in flow cytometry as more events at higher side scatter and forward scatter. To further determine whether such a change in the cell event distribution is related to HSC differentiation, HSC markers and differentiation markers of in vitro-cultured HSC were detected by flow cytometry at different side scatter and forward scatter levels using modified ISHAGE gating strategies.nbsp The results revealed that cultured HSC with higher side scatter have a lower percentage of cells positive for HSC markers and a higher percentage of differentiation makers, while those with higher forward scatter have a higher percentage of differentiation makers but a slightly higher percentage of stem cell markers, suggesting that side scatter and forward scatter levels of cultured HSC correlate with the differentiation level of these cells.

وصف الملف: application/pdf

العلاقة: https://www.neliti.com/publications/262749/assessment-of-differentiation-states-of-hematopoietic-stem-cells-following-in-viTest

الإتاحة: https://www.neliti.com/publications/262749/assessment-of-differentiation-states-of-hematopoietic-stem-cells-following-in-viTest

المؤلفون: Peeters S. A., Engelen L., Buijs J., Chaturvedi N., Fuller J. H., Schalkwijk C. G., Stehouwer C. D., Karamanos B., Kofinis A., Petrou K., Giorgino F., Picca G., Angarano A., de Pergola. G., Laviola L., Giorgino R., Ionescu-Tirgoviste C., Coszma A., Guja C., Songini M., Casu A., Pedron M., Pintus S., Fossarello M., Ferriss J. B., Grealy G., O'Keefe D., Toeller M., Arden C., Rottiers R., Tuyttens C., Priem H., Ebeling P., Kylliainen M., Koivisto V. A., Idzior-Walus B., Sieradzki J., Cyganek K., Solnica B., Lemkes H. H. P. J., Lemkes-Stuffken J. C., Nunes-Correa J., Rogado M. C., Gardete-Correia L., Cardoso M. C., Silva A., Boavida J., Machado Sa Marques M., Michel G., Wirion R., Cardillo S., Pozza G., Mangili R., Asnaghi V., Standl E., Schaffler B., Brand H., Harms A., Ben Soussan M., Verier-Mine O., Fallas P., Fallas M. C., Holloway J., Asbury L., Betteridge D. J., Cathelineau G., Bouallouche A., Villatte Cathelineau B., Santeusanio F., Rosi G., D'Alessandro V., Cagini C., Bottini P., Reboldi G. P., Navalesi R., Penno G., Bandinelli S., Miccoli R., Nannipieri M., Ghirlanda G., Saponara C., Cotroneo P., Manto A., Minnella A., Ward J. D., Tesfaye S., Eaton S., Mody C., Borra M., Cavallo Perin P., Giunti S., Grassi G., Pagano G. F., Porta M., Sivieri R., Vitelli F., Veglio M., Papazoglou N., Manes G., Muggeo M., Iagulli M., Cacciatori V., Cattedra di Malattie del Metabolismo V., Irsigler K., Abrahamian H., Walford S., Sinclair J., Hughes S., McLelland V., Ward J., Roglic G., Metelko Z., Pepeonik Z. R.

المساهمون: Peeters, S. A., Engelen, L., Buijs, J., Chaturvedi, N., Fuller, J. H., Schalkwijk, C. G., Stehouwer, C. D., Karamanos, B., Kofinis, A., Petrou, K., Giorgino, F., Picca, G., Angarano, A., Pergola. G., De, Laviola, L., Giorgino, R., Ionescu-Tirgoviste, C., Coszma, A., Guja, C., Songini, M., Casu, A., Pedron, M., Pintus, S., Fossarello, M., Ferriss, J. B., Grealy, G., O'Keefe, D., Toeller, M., Arden, C., Rottiers, R., Tuyttens, C., Priem, H., Ebeling, P., Kylliainen, M., Koivisto, V. A., Idzior-Walus, B., Sieradzki, J., Cyganek, K., Solnica, B., Lemkes, H. H. P. J., Lemkes-Stuffken, J. C., Nunes-Correa, J., Rogado, M. C., Gardete-Correia, L., Cardoso, M. C., Silva, A., Boavida, J., Machado Sa Marques, M., Michel, G., Wirion, R., Cardillo, S., Pozza, G., Mangili, R., Asnaghi, V., Standl, E., Schaffler, B., Brand, H., Harms, A., Ben Soussan, M., Verier-Mine, O., Fallas, P., Fallas, M. C., Holloway, J., Asbury, L., Betteridge, D. J., Cathelineau, G., Bouallouche, A., Villatte Cathelineau, B., Santeusanio, F., Rosi, G., D'Alessandro, V., Cagini, C., Bottini, P., Reboldi, G. P., Navalesi, R., Penno, G., Bandinelli, S., Miccoli, R., Nannipieri, M., Ghirlanda, G., Saponara, C., Cotroneo, P., Manto, A., Minnella, A., Ward, J. D., Tesfaye, S., Eaton, S., Mody, C., Borra, M., Cavallo Perin, P., Giunti, S., Grassi, G., Pagano, G. F., Porta, M., Sivieri, R., Vitelli, F., Veglio, M., Papazoglou, N., Manes, G.

مصطلحات موضوعية: Albuminuria, Cardiovascular disease, Matrix metalloproteinase, Retinopathy, Tissue inhibitor of metalloproteinase, Type 1 diabete, Adult, Biomarker, Cohort Studie, Cross-Sectional Studie, Diabetes Complication, Diabetes Mellitus, Type 1, Europe, Female, Human, Male, Matrix Metalloproteinase 10, Matrix Metalloproteinase 2, Matrix Metalloproteinase 3, Middle Aged, Prospective Studie, Tissue Inhibitor of Metalloproteinase-1, Vascular Diseases

الوصف: Impaired regulation of extracellular matrix remodeling by matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase (TIMP) may contribute to vascular complications in patients with type 1 diabetes. We investigated associations between plasma MMP-1, -2, -3, -9, -10 and TIMP-1, and cardiovascular disease (CVD) or microvascular complications in type 1 diabetic patients. We also evaluated to which extent these associations could be explained by low-grade inflammation (LGI) or endothelial dysfunction (ED). Methods: 493 type 1 diabetes patients (39.5 ± 9.9 years old, 51% men) from the EURODIAB Prospective Complications Study were included. Linear regression analysis was applied to investigate differences in plasma levels of MMP-1, -2, -3, -9, -10, and TIMP-1 between patients with and without CVD, albuminuria or retinopathy. All analyses were adjusted for age, sex, duration of diabetes, Hba1c and additionally for other cardiovascular risk factors including LGI and ED. Results: Patients with CVD (n = 118) showed significantly higher levels of TIMP-1 [β = 0.32 SD (95%CI: 0.12; 0.52)], but not of MMPs, than patients without CVD (n = 375). Higher plasma levels of MMP-2, MMP-3, MMP-10 and TIMP-1 were associated with higher levels of albuminuria (p-trends were 0.028, 0.004, 0.005 and 0.001, respectively). Severity of retinopathy was significantly associated with higher levels of MMP-2 (p-trend = 0.017). These associations remained significant after further adjustment for markers of LGI and ED. Conclusions: These data support the hypothesis that impaired regulation of matrix remodeling by actions of MMP-2, -3 and-10 and TIMP-1 contributes to the pathogenesis of vascular complications in type 1 diabetes.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/25848912; info:eu-repo/semantics/altIdentifier/wos/WOS:000350726000001; volume:14; issue:1; firstpage:31; journal:CARDIOVASCULAR DIABETOLOGY; http://hdl.handle.net/11586/353539Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84925234995

الإتاحة: https://doi.org/10.1186/s12933-015-0195-2Test
http://hdl.handle.net/11586/353539Test

المؤلفون: DE BONA C., ZAPPELLA M., HAYEK G., MELONI, ILARIA, VITELLI F., BRUTTINI M., CUSANO R., LOFFREDO P., LONGO I., RENIERI, ALESSANDRA

المساهمون: DE BONA, C., Zappella, M., Hayek, G., Meloni, Ilaria, Vitelli, F., Bruttini, M., Cusano, R., Loffredo, P., Longo, I., Renieri, Alessandra

مصطلحات موضوعية: MECP2, Preserved speech variant, Rett syndrome

الوصف: Rett syndrome is a neurological disorder affecting predominantly females with regression loss of speech and purposeful hand use, after a few months of almost normal development. Postnatal microcephaly, hand dispraxia, stereotypic 'hand-washing' activities, ataxia, and abnormal breathing are among its most characteristic features. Another aspect of this disorder is growth failure. The preserved speech variant (PSV) shares with Rett syndrome the same course and the stereotypic hand-washing activities but it differs in that patients typically recover some degree of speech and hand use and usually do not show growth failure. Progressive scoliosis, epilepsy and other minor handicaps, usually present in Rett syndrome, are rare in the preserved speech variant. Here we explore the spectrum of mutations affecting the MECP2 gene in a group of 25 classic Rett syndrome girls and in three patients with the preserved speech variant. Among the Rett syndrome group, two novel mutational hot spots (R270X and R294X), four novel mutations, two novel small deletions, as well as the previously reported 806delG, R168X and R255X mutations, were identified in 20/25 patients. Of note, among the preserved speech variants, two patients carry deletions of 41 bp and 44 bp each, which are strikingly similar to those observed in classic Rett syndrome. Our results confirm the presence of mutational hot spots in MECP2, broaden the spectrum of mutations, pinpoint additional mutational hot spots and establish that the preserved speech variant is indeed allelic of the classic form. Phenotype variability is only partially dependent on the kind of MECP2 mutation and other mechanisms such as skewed X-inactivation, and/or modifier gene effects should be investigated to explain the variable recovery in speech and hand use.

وصف الملف: STAMPA

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/10854091; info:eu-repo/semantics/altIdentifier/wos/WOS:000087472900002; volume:8; issue:5; firstpage:325; lastpage:330; numberofpages:6; journal:EUROPEAN JOURNAL OF HUMAN GENETICS; http://hdl.handle.net/11365/37864Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0034088550; https://www.nature.com/articles/5200473Test

الإتاحة: https://doi.org/10.1038/sj.ejhg.5200473Test
http://hdl.handle.net/11365/37864Test
https://www.nature.com/articles/5200473Test

المؤلفون: Ghidini, N., Brausi, M., Piazza, R., Gatti, L., Vitelli, F., Rabito, S., Ferrari, G.

المصدر: European Urology Supplements ; volume 18, issue 9, page e3246 ; ISSN 1569-9056

مصطلحات موضوعية: Urology

الإتاحة: https://doi.org/10.1016/s1569-9056Test(19)33628-0
https://api.elsevier.com/content/article/PII:S1569905619336280?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1569905619336280?httpAccept=text/plainTest

المؤلفون: Vitelli, F., Botticelli, L., Peracchia, G., Ferrari, R., Brausi, M.

المصدر: European Urology Supplements ; volume 18, issue 9, page e3240 ; ISSN 1569-9056

مصطلحات موضوعية: Urology

الإتاحة: https://doi.org/10.1016/s1569-9056Test(19)33620-6
https://api.elsevier.com/content/article/PII:S1569905619336206?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1569905619336206?httpAccept=text/plainTest

المؤلفون: Vitelli, F.

المصدر: Human Molecular Genetics ; volume 12, issue 16, page 2041-2048 ; ISSN 1460-2083

مصطلحات موضوعية: Genetics (clinical), Genetics, Molecular Biology, General Medicine

الإتاحة: https://doi.org/10.1093/hmg/ddg216Test
http://academic.oup.com/hmg/article-pdf/12/16/2041/6947780/ddg216.pdfTest

المؤلفون: Meloni, I, Vitelli, F, Pucci, L, Lowry, R B, Tonlorenzi, R, Rossi, E, Ventura, M, Rizzoni, G, Kashtan, C E, Pober, B, Renieri, A

مصطلحات موضوعية: Letters to JMG

وصف الملف: text/html

العلاقة: http://jmg.bmj.com/cgi/content/short/39/5/359Test; http://dx.doi.org/10.1136/jmg.39.5.359Test

الإتاحة: https://doi.org/10.1136/jmg.39.5.359Test
http://jmg.bmj.com/cgi/content/short/39/5/359Test