المؤلفون: Norberto Guelbert, Oscar Mauricio Espitia Segura, Carolina Amoretti, Angélica Arteaga Arteaga, Nora Graciela Atanacio, Sabrina Bazan Natacha, Ellaine Doris Fernandes Carvalho, Maria Denise Fernandes Carvalho de Andrade, Inés María Denzler, Consuelo Durand, Erlane Ribeiro, Juan Carlos Giugni, Gabriel González, Dolores González Moron, Guillermo Guelbert, Zulma Janneth Hernández Rodriguez, Katiane Embiruçu Emilia, Marcelo Andrés Kauffman, Nury Isabel Mancilla, Laureano Marcon, Alessandra Marques Pereira, Carolina Fischinger Moura de Souza, Victor Adrián Muñoz, Ricardo Andrés Naranjo Flórez, André Luiz Pessoa, María Victoria Ruiz, Martha Luz Solano Villareal, Norma Spécola, Lina Marcela Tavera, Javiera Tello, Mónica Troncoso Schifferli, Sonia Ugrina, María Magdalena Vaccarezza, Diane Vergara, María Mercedes Villanueva

المصدر: Molecular Genetics and Metabolism Reports, Vol 38, Iss , Pp 101060- (2024)

مصطلحات موضوعية: Batten disease, Enzyme replacement therapy, Epilepsy, Language delay, Ataxia, Cerliponase alfa, Medicine (General), R5-920, Biology (General), QH301-705.5

الوصف: Introduction: Late infantile neuronal ceroid lipofuscinosis type 2 (CLN2), is a neurodegenerative autosomal recessive disease caused by TPP1 gene variants, with a spectrum of classic and atypical phenotypes. The aim of treatment is to slow functional decline as early as possible in an attempt to improve quality of life and survival. This study describes the clinical characteristics as well as the response to treatment with cerliponase alfa. Materials and methods: A retrospective study was conducted in five Latin-American countries, using clinical records from patients with CLN2. Clinical follow-up and treatment variables are described. A descriptive and bivariate statistical analysis was performed. Results: A total of 36 patients were observed (range of follow-up of 61–110 weeks post-treatment). At presentation, patients with the classic phenotype (n = 16) exhibited regression in language (90%), while seizures were the predominant symptom (87%) in patients with the atypical phenotype (n = 20). Median age of symptom onset and time to first specialized consultation was 3 (classical) and 7 (atypical) years, while the median time interval between onset of symptoms and treatment initiation was 4 years (classical) and 7.5 (atypical). The most frequent variant was c.827 A > T in 17/72 alleles, followed by c.622C > T in 6/72 alleles. All patients were treated with cerliponase alfa, and either remained functionally stable or had a loss of 1 point on the CLN2 scale, or up to 2 points on the Wells Cornel and Hamburg scales, when compared to pretreatment values. Discussion and conclusion: This study reports the largest number of patients with CLN2 currently on treatment with cerliponase alfa in the world. Data show a higher frequency of patients with atypical phenotypes and a high allelic proportion of intron variants in our region. There was evidence of long intervals until first specialized consultation, diagnosis, and enzyme replacement therapy. Follow-up after the initiation of cerliponase alfa showed slower progression or stabilization of the disease, associated with adequate clinical outcomes and stable functional scores. These improvements were consistent in both clinical phenotypes.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2214426924000132Test; https://doaj.org/toc/2214-4269Test

الوصول الحر: https://doaj.org/article/d1f6513168dd43cd873e78c97646f8bfTest

المؤلفون: Osmar Antonio Jaramillo-Morales, Erik Díaz-Cervantes, Lisa Dalla Via, Aida Nelly Garcia-Argaez, Josué Vidal Espinosa-Juárez, José Carlos Ovando-Zambrano, Victor Manuel Muñoz-Pérez, Carmen Valadez-Vega, Mirandeli Bautista

المصدر: Scientia Pharmaceutica, Vol 91, Iss 3, p 40 (2023)

مصطلحات موضوعية: verbascoside, hepatoprotective effect, Leucophyllum frutescens, thioacetamide, Pharmacy and materia medica, RS1-441

الوصف: There is an urgent need for scientists to verify the pharmacological properties of medicinal plants. Leucophyllum frutescens (Lf) belongs to the family Scrophulariaceae, and it is used in the treatment of airway diseases such as cough, tuberculosis, and asthma. The methanolic extract of the aerial parts of Lf allows for the isolation and identification of verbascoside (Vb). This study aimed to evaluate the hepatoprotective effect of Vb, a caffeoyl phenylethanoid glycoside (CPG), on post-necrotic liver damage induced by thioacetamide (TA) via in vivo and in silico studies, with the latter considering a cancerous process. The aerial parts of Lf were extracted by maceration using hexane methanol (5 L/500 g/8 days). Vb was isolated from methanol extract at approximately 30%. Wistar rats were intragastrically pretreated or not with a single dose of Vb (20 mg/kg) for four days. On the fourth day, a single dose of TA (6.6 mmol/kg) was intraperitoneally injected. Blood samples and parameters related to liver damage, like AST and ALT, were obtained. Vb significantly reduced the level of liver injury following thioacetamide-induced necrosis. This was corroborated by in silico assay and docking studies, demonstrating that Vb can interact with a HeLa target through hydrogen bonds and electrostatic interactions, achieving better performance than commercial chemotherapeutic Taxol®, by 0.34 kcal/mol. AST and ALT were significantly lower in the rats pretreated with Vb. Furthermore, Vb did not induce cytotoxicity and had a median lethal dose (LD50) greater than 5000 mg/kg. These results suggest that Vb may be used as an alternative to reduce liver damage.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2218-0532/91/3/40Test; https://doaj.org/toc/0036-8709Test; https://doaj.org/toc/2218-0532Test

الوصول الحر: https://doaj.org/article/a26c0a636d834f02b609d4822ca17284Test

المؤلفون: Jorge Paz-Gutierrez, Byron A. Portilla-Benavides, William F. Bermudez-Bravo, Victor E. Muñoz-Martinez

المصدر: Journal of the Mexican Federation of Radiology and Imaging, Vol 2, Iss 1 (2023)

مصطلحات موضوعية: Diffusion tensor imaging. Anisotropy factor. Apparent diffusion coefficient. Cervical spinal cord., Medical physics. Medical radiology. Nuclear medicine, R895-920

الوصف: Introduction: Diffusion tensor imaging measurements have not been standardized nor normal values defined at different cervical spinal cord levels. This study determined the normal quantitative values of diffusion tensor imaging with 3.0T MRI in the cervical spinal cord and their relationship with sex and age in a Mexican population. Material and Methods: Patients of both sexes over 18 years of age without cervical spinal cord symptoms and normal findings on conventional 3.0T MRI were included. Quantitative fractional anisotropy (FA), apparent diffusion coefficient (ADC), and mean diffusivity (MD) values in the 4-mm region of interest (ROI) of the complete cervical spinal cord were determined. Statistical analysis was performed using ANOVA. A p-value < 0.05 was significant. Results: Seventy-three participants with a mean age of women of 53 ± 17 years and men of 48.9 ± 16.4 years (p: not significant) were included. The number of participants per age group was comparable for both sexes. The mean FA was 0.679 ± 0.120, ADC was 0.879 ± 0.157, and MD was 0.123 ± 0.038 of the whole cervical spinal cord. FA and MD values decreased at lower cervical spine levels (p < 0.001). In contrast, ADC increased in the lower cervical segment due to a higher diffusion capacity of water molecules (p < 0.001). No statistically significant differences were found for the mean values FA and ADC with respect to sex and age in the whole cervical spinal cord. Conclusion: This study is the first to determine normal quantitative FA, ADC, and MD values using diffusion tensor imaging with 3.0T MRI of the complete cervical spinal cord in an adult Mexican population. Diffusion tensor imaging measurements were not affected by sex or age.

وصف الملف: electronic resource

العلاقة: https://www.jmexfri.com/frame_esp.php?id=46Test; https://doaj.org/toc/2938-1215Test; https://doaj.org/toc/2696-8444Test

الوصول الحر: https://doaj.org/article/4fc05c68adb941fd9e49ceed819382f5Test

المؤلفون: Ángel Chávez-Salazar, Américo Guevara-Pérez, Christian R. Encina-Zelada, Pierre Vidaurre-Rojas, Víctor Hugo Muñoz Delgado

مصطلحات موضوعية: Science of Cocoa and Chocolate Production, Food Science, Agricultural and Biological Sciences, Life Sciences, Fermentation, Theobroma, Horticulture, Chemistry, Biology

الوصف: El estudio evaluó el efecto de la altura del cajón de fermentación y los métodos de secado en las características fisicoquímicas del cacao CCN 51. Se procesaron mazorcas de cacao mediante cajón de fermentación y se secaron al sol, con un secador solar o con flujo de aire caliente. Se encontró que la menor degradación de polifenoles (16,9%) se produjo a 1m de altura y la menor pérdida de antocianinas (42,4%) a 0,20m. Después de 96 horas de fermentación, las muestras alcanzaron una temperatura promedio de 52,3°C y un pH final de 4,31. Los sólidos solubles disminuyeron de 19,5 a 0 °Brix después de tres días de fermentación. Respecto a los métodos de secado, el flujo de aire caliente produjo el menor porcentaje de degradación de polifenoles (29,9%) y antocianinas (65,1%). Las muestras secadas al sol y las secadas con el secador solar presentaron diferencias significativas en la degradación de polifenoles. El método de secado seleccionado fue con flujo de aire caliente, y las muestras presentaron las siguientes ... : قيمت الدراسة تأثير ارتفاع صندوق التخمير وطرق التجفيف على الخصائص الفيزيائية الكيميائية للكاكاو CCN 51. تمت معالجة أكواز الكاكاو عن طريق درج التخمير وتجفيفها في الشمس، باستخدام مجفف شمسي أو مع تدفق الهواء الساخن. وجد أن أقل تحلل للبوليفينول (16.9 ٪) حدث على ارتفاع 1 متر وأقل خسارة للأنثوسيانين (42.4 ٪) عند 0.20 متر. بعد 96 ساعة من التخمير، وصلت العينات إلى متوسط درجة حرارة 52.3 درجة مئوية ودرجة الحموضة النهائية 4.31. انخفضت المواد الصلبة القابلة للذوبان من 19.5 إلى 0 درجةبريكس بعد ثلاثة أيام من التخمير. فيما يتعلق بطرق التجفيف، أنتج تدفق الهواء الساخن أقل نسبة من تحلل البوليفينول (29.9 ٪) والأنثوسيانين (65.1 ٪). أظهرت العينات المجففة في الشمس وتلك المجففة بالمجفف الشمسي اختلافات كبيرة في تحلل البوليفينول. كانت طريقة التجفيف المختارة بتدفق الهواء الساخن، وقدمت العينات الخصائص التالية: 8.13(جم EAG/100 جم بالمتر المربع)، 0.64 (مجم سيانيدين-3 - جلوكوزيد/جم بالمتر المربع)، 0.32(جم حمض الخليك /100 جم بالمتر المربع). ...

العلاقة: https://dx.doi.org/10.60692/412t4-51634Test

الإتاحة: https://doi.org/10.60692/mxxj4-by95410.60692/412t4-51634Test

المؤلفون: Giuseppe De Luca, Magdy Algowhary, Berat Uguz, Dinaldo C. Oliveira, Vladimir Ganyukov, Oliver Busljetik, Miha Cercek, Lisette Okkels Jensen, Poay Huan Loh, Lucian Calmac, Gerard Roura i Ferrer, Alexandre Quadros, Marek Milewski, Fortunato Scotto D’Uccio, Clemens von Birgelen, Francesco Versaci, Jurrien Ten Berg, Gianni Casella, Aaron Wong Sung Lung, Petr Kala, José Luis Díez Gil, Xavier Carrillo, Maurits Dirksen, Victor Becerra Munoz, Michael Kang-yin Lee, Dafsah Arifa Juzar, Rodrigo de Moura Joaquim, Roberto Paladino, Davor Milicic, Periklis Davlouros, Nikola Bakraceski, Filippo Zilio, Luca Donazzan, Adriaan Kraaijeveld, Gennaro Galasso, Lux Arpad, Lucia Marinucci, Vincenzo Guiducci, Maurizio Menichelli, Alessandra Scoccia, Aylin Hatice Yamac, Kadir Ugur Mert, Xacobe Flores Rios, Tomas Kovarnik, Michal Kidawa, Josè Moreu, Vincent Flavien, Enrico Fabris, Iñigo Lozano Martínez-Luengas, Marco Boccalatte, Francisco Bosa Ojeda, Carlos Arellano-Serrano, Gianluca Caiazzo, Giuseppe Cirrincione, Hsien-Li Kao, Juan Sanchis Forés, Luigi Vignali, Helder Pereira, Stephane Manzo-Silberman, Santiago Ordoñez, Alev Arat Özkan, Bruno Scheller, Heidi Lehitola, Rui Teles, Christos Mantis, Ylitalo Antti, João António Brum Silveira, Cesar Rodrigo Zoni, Ivan Bessonov, Giuseppe Uccello, George Kochiadakis, Dimitrios Alexopulos, Carlos E. Uribe, John Kanakakis, Benjamin Faurie, Gabriele Gabrielli, Alejandro Gutierrez Barrios, Juan Pablo Bachini, Alex Rocha, Frankie C. C. Tam, Alfredo Rodriguez, Antonia Anna Lukito, Veauthyelau Saint-Joy, Gustavo Pessah, Andrea Tuccillo, Alfonso Ielasi, Giuliana Cortese, Guido Parodi, Mohammed Abed Burgadha, Elvin Kedhi, Pablo Lamelas, Harry Suryapranata, Matteo Nardin, Monica Verdoia

المصدر: Journal of Clinical Medicine; Volume 12; Issue 6; Pages: 2116

مصطلحات موضوعية: ageing, ST-segment elevation myocardial infarction, COVID-19

الوصف: Background: The constraints in the management of patients with ST-segment elevation myocardial infarction (STEMI) during the COVID-19 pandemic have been suggested to have severely impacted mortality levels. The aim of the current analysis is to evaluate the age-related effects of the COVID-19 pandemic on mechanical reperfusion and 30-day mortality for STEMI within the registry ISACS-STEMI COVID-19. Methods: This retrospective multicenter registry was performed in high-volume PPCI centers on four continents and included STEMI patients undergoing PPCI in March–June 2019 and 2020. Patients were divided according to age (< or ≥75 years). The main outcomes were the incidence and timing of PPCI, (ischemia time longer than 12 h and door-to-balloon longer than 30 min), and in-hospital or 30-day mortality. Results: We included 16,683 patients undergoing PPCI in 109 centers. In 2020, during the pandemic, there was a significant reduction in PPCI as compared to 2019 (IRR 0.843 (95%-CI: 0.825–0.861, p < 0.0001). We found a significant age-related reduction (7%, p = 0.015), with a larger effect on elderly than on younger patients. Furthermore, we observed significantly higher 30-day mortality during the pandemic period, especially among the elderly (13.6% vs. 17.9%, adjusted HR (95% CI) = 1.55 [1.24–1.93], p < 0.001) as compared to younger patients (4.8% vs. 5.7%; adjusted HR (95% CI) = 1.25 [1.05–1.49], p = 0.013), as a potential consequence of the significantly longer ischemia time observed during the pandemic. Conclusions: The COVID-19 pandemic had a significant impact on the treatment of patients with STEMI, with a 16% reduction in PPCI procedures, with a larger reduction and a longer delay to treatment among elderly patients, which may have contributed to increase in-hospital and 30-day mortality during the pandemic.

وصف الملف: application/pdf

العلاقة: Cardiology; https://dx.doi.org/10.3390/jcm12062116Test

الإتاحة: https://doi.org/10.3390/jcm12062116Test

المؤلفون: Ivan J. Núñez-Gil, Gisela Feltes, María C. Viana-Llamas, Sergio Raposeiras-Roubin, Rodolfo Romero, Emilio Alfonso-Rodríguez, Aitor Uribarri, Francesco Santoro, Víctor Becerra-Muñoz, Martino Pepe, Alex F. Castro-Mejía, Jaime Signes-Costa, Adelina Gonzalez, Francisco Marín, Javier Lopez-País, Enrico Cerrato, Olalla Vázquez-Cancela, Carolina Espejo-Paeres, Álvaro López Masjuan, Lazar Velicki, Ibrahim El-Battrawy, Harish Ramakrishna, Antonio Fernandez-Ortiz, Julián Perez-Villacastín

المصدر: Journal of Clinical Medicine; Volume 12; Issue 2; Pages: 706

مصطلحات موضوعية: COVID-19, mortality, cardiology, persistent, prognosis, heart disease

الوصف: Background: Heart disease is linked to worse acute outcomes after coronavirus disease 2019 (COVID-19), although long-term outcomes and prognostic factor data are lacking. We aim to characterize the outcomes and the impact of underlying heart diseases after surviving COVID-19 hospitalization. Methods: We conducted an analysis of the prospective registry HOPE-2 (Health Outcome Predictive Evaluation for COVID-19-2, NCT04778020). We selected patients discharged alive and considered the primary end-point all-cause mortality during follow-up. As secondary main end-points, we included any readmission or any post-COVID-19 symptom. Clinical features and follow-up events are compared between those with and without cardiovascular disease. Factors with p < 0.05 in the univariate analysis were entered into the multivariate analysis to determine independent prognostic factors. Results: HOPE-2 closed on 31 December 2021, with 9299 patients hospitalized with COVID-19, and 1805 died during this acute phase. Finally, 7014 patients with heart disease data were included in the present analysis, from 56 centers in 8 countries. Heart disease (+) patients were older (73 vs. 58 years old), more frequently male (63 vs. 56%), had more comorbidities than their counterparts, and suffered more frequently from post-COVID-19 complications and higher mortality (OR heart disease: 2.63, 95% CI: 1.81–3.84). Vaccination was found to be an independent protector factor (HR all-cause death: 0.09; 95% CI: 0.04–0.19). Conclusions: After surviving the acute phase, patients with underlying heart disease continue to present a more complex clinical profile and worse outcomes including increased mortality. The COVID-19 vaccine could benefit survival in patients with heart disease during follow-up.

وصف الملف: application/pdf

العلاقة: Cardiovascular Medicine; https://dx.doi.org/10.3390/jcm12020706Test

الإتاحة: https://doi.org/10.3390/jcm12020706Test

المؤلفون: Juan M. Herrera-López, Álvaro Galán-Cuenca, Isabel García-Morales, Marcos Rollón, Irene Rivas-Blanco, Víctor F. Muñoz

المصدر: Revista Iberoamericana de Automática e Informática Industrial RIAI, Vol 21, Iss 1, Pp 69-80 (2023)

مصطلحات موضوعية: cirugía laparoscópica, simulador quirúrgico, sistema cíber-físico, robot manipulador redundante, modelo cinemático inverso, adquisición de vídeo, Control engineering systems. Automatic machinery (General), TJ212-225

الوصف: Este trabajo desarrolla la implantación de un entorno de trabajo cíber-físico para cirugía laparoscópica que permite utilizar un simulador quirúrgico con un brazo robótico, para así facilitar el aprendizaje y la investigación con este tipo de sistemas. Se propone y valida una configuración para cada uno de los elementos del quirófano que permite cumplir todas las restricciones funcionales. También se implanta un Modelo Cinemático Inverso para el brazo robótico redundante que devuelve la solución más adecuada que asegura el cumplimiento de estas restricciones. El entorno de trabajo se ha implementado haciendo uso de ROS y MATLAB, realizando una serie de pruebas a fin de validar el desarrollo de aplicaciones utilizando este framework.

العلاقة: https://polipapers.upv.es/index.php/RIAI/article/view/18753Test; https://doaj.org/toc/1697-7912Test; https://doaj.org/toc/1697-7920Test; https://doaj.org/article/76511166e6034e36a68ab506eabadfa8Test

الإتاحة: https://doi.org/10.4995/riai.2023.18753Test
https://doaj.org/article/76511166e6034e36a68ab506eabadfa8Test

المؤلفون: Fabiola González-Castelazo, Luis E. Soria-Jasso, Ivan Torre-Villalvazo, Raquel Cariño-Cortés, Víctor M. Muñoz-Pérez, Mario I. Ortiz, Eduardo Fernández-Martínez

المصدر: Plants, Vol 12, Iss 3583, p 3583 (2023)

مصطلحات موضوعية: diabetes, dyslipidemias, hypertension, medicinal plants, metabolic syndrome, Rubiaceae, Botany, QK1-989

الوصف: Metabolic syndrome (MetS) predisposes individuals to chronic non-communicable diseases (NCDs) like type 2 diabetes (T2D), non-alcoholic fatty liver disease, atherosclerosis, and cardiovascular disorders caused by systemic inflammation, intestinal dysbiosis, and diminished antioxidant ability, leading to oxidative stress and compromised insulin sensitivity across vital organs. NCDs present a global health challenge characterized by lengthy and costly pharmacological treatments. Complementary and alternative medicine using herbal therapies has gained popularity. Approximately 350,000 plant species are considered medicinal, with 80% of the world’s population opting for traditional remedies; however, only 21,000 plants are scientifically confirmed by the WHO. The Rubiaceae family is promissory for preventing and treating MetS and associated NCDs due to its rich content of metabolites renowned for their antioxidative, anti-inflammatory, and metabolic regulatory properties. These compounds influence transcription factors and mitigate chronic low-grade inflammation, liver lipotoxicity, oxidative stress, and insulin resistance, making them a cost-effective non-pharmacological approach for MetS prevention and treatment. This review aims to collect and update data that validate the traditional uses of the Rubiaceae family for treating MetS and associated NCDs from experimental models and human subjects, highlighting the mechanisms through which their extracts and metabolites modulate glucose and lipid metabolism at the molecular, biochemical, and physiological levels.

العلاقة: https://www.mdpi.com/2223-7747/12/20/3583Test; https://doaj.org/toc/2223-7747Test; https://doaj.org/article/204ef929a11c46fe9b61edc35aa0dfceTest

الإتاحة: https://doi.org/10.3390/plants12203583Test
https://doaj.org/article/204ef929a11c46fe9b61edc35aa0dfceTest

المؤلفون: Luis Vega López, Sonia Lareu Vidal, Gonzalo Anes González, Víctor Álvarez Muñoz

المصدر: Anales de Pediatría (English Edition), Vol 98, Iss 6, Pp 481-482 (2023)

مصطلحات موضوعية: Pediatrics, RJ1-570

العلاقة: http://www.sciencedirect.com/science/article/pii/S2341287923000947Test; https://doaj.org/toc/2341-2879Test; https://doaj.org/article/84dbb0e09d18439d9ef7e0190304f030Test

الإتاحة: https://doi.org/10.1016/j.anpede.2023.04.003Test
https://doaj.org/article/84dbb0e09d18439d9ef7e0190304f030Test

المؤلفون: Stefano Dalle Palle, Elisa Boscari, Simone Giulio Bordignon, Víctor Hugo Munoz-Mora, Giorgio Bertorelle, Leonardo Congiu

المساهمون: Dalle Palle, Stefano, Boscari, Elisa, Giulio Bordignon, Simone, Hugo Munoz-Mora, Víctor, Bertorelle, Giorgio, Congiu, Leonardo

مصطلحات موضوعية: acipensaeridae, Adriatic sturgeon, allopolyploid, autotertraploid, disomic segregation, inheritance, microsatellite, tetrasomic segregation

الوصف: The Adriatic sturgeon, Acipenser naccarii (Bonaparte, 1836), is a critically endangered tetraploid endemism of the Adriatic region; it has been targeted, over the last 20 years, by different conservation programs based on controlled reproduction of captive breeders followed by the release of their juvenile offspring; its preservation would greatly benefit from the correct and coordinated management of the residual genetic variability available in the different captive stocks. In this sense, the setup of an efficient parental allocation procedure would allow identifying familiar groups and establishing informed breeding plans, effectively preserving genetic variation. However, being the species tetraploid, the analyses often deal with complex genome architecture and a preliminary evaluation of allele segregation patterns at different chromosomes is necessary to assess whether the species can be considered a pure tetraploid, as previously observed at some loci, or if a more complex situation is present. Here we study the segregation at 14 microsatellites loci in 12 familiar groups. Results support in different families the tetrasomic segregation pattern at 11 markers and the disomic segregation at three markers. The Adriatic sturgeon thus shows a mixed inheritance modality. In this species, and likely in other sturgeons, accurate knowledge of the loci used for paternity analysis is therefore required.

وصف الملف: STAMPA

العلاقة: info:eu-repo/semantics/altIdentifier/wos/WOS:000857675000001; volume:14; issue:9; firstpage:745-1; lastpage:745-16; numberofpages:16; journal:DIVERSITY; https://hdl.handle.net/11392/2497737Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85138682962; https://www.mdpi.com/1424-2818/14/9/745Test

الإتاحة: https://doi.org/10.3390/d14090745Test
https://hdl.handle.net/11392/2497737Test
https://www.mdpi.com/1424-2818/14/9/745Test