المؤلفون: Viana, Altevir Rossato

مرشدي الرسالة: Krause, Luciana Maria Fontanari, Schuch, André Passaglia, Araújo, Maria do Carmo dos Santos

المصدر: Repositório Institucional Universidade FranciscanaUniversidade FranciscanaUFN.

مصطلحات موضوعية: Antioxidante, Antitumoral, Citotoxicidade, Solanaceae, C57BL/6., Antioxidant, Antitumor, Cytotoxicity, Solanaceae, C57BL/6, Ciências da Saúde e da Vida

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Cancer is the second largest cause of death in the world, only behind cardiovascular disease. This pathology is able to circumvent mechanisms of proliferative suppression, growing and surviving in hostile environments. Melanoma is a type of malignant tumor, has high metastatic potential and may be resistant to conventional anticancer agents. Although it does not correspond to the most frequent type of neoplasia, it represents a high mortality rate because of its capacity of metastases and as untreated difficulties. Due to these factors, the search for alternatives is highly encouraged. Many of the anticancer medicaments have their origin from natural, semi-synthetic and synthetic products. In Brazil, due to the richness of the national flora and the lack of knowledge about its bioactive products, the projects that seek this knowledge are important. The objective of the present study was to verify the antitumor activity in vitro and in vivo of a plant of the family Solanaceae, present in Rio Grande do Sul. Vassobia breviflora is known for its antitumor potential, mainly due to the presence of vitanolides, Withaferin A (WA). However, the studies found with the Brazilian plant are scarce. The vegetal species used in the work was extracted from a private property in the region of Boca do Monte, in the municipality of Santa Maria, RS. It was cataloged by the herbarium of the Federal University of Santa Maria and its aqueous extract was prepared with the dried leaves, extracted at room temperature. Controls were made using the commercially acquired WA active principle and the standard chemotherapy for melanoma, Temozolomide. Verification of the amount of WA was conducted by high performance liquid chromatography (HPLC). The tests for the detection of antioxidant compounds in the extract were made from the analysis of total phenols, diammonium salt - 2,20-azino-bis (acid 3-ethylbenzthiazoline-6-sulfônico) (ABTS) e 2,2-diphenyl-1-picrylhydrazil (DPPH). The cytotoxic potential was investigated from the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT), in B16F10 cells. In vivo tests were conducted in the C57BL/6 line of isogenic mice, inoculated subcutaneously with the B16F10 line melanoma and treated for 30 days orally with the different treatments. The project was approved by the institution's animal ethics committee. During the treatment period, the weights of the animals were checked weekly with the aid of an analytical balance. The volume of the tumor by the measurement of the tumor mass, with a digital caliper and the survival time by the Kaplan Meyer curve. After the euthanasia of the animals, they had the tumors removed and weighed. In addition, other organs, such as spleen, lymph node, liver, lung, brain and kidney were removed and fixed for later histopathological analysis, and properly conditioned and frozen for biochemical and gene analyzes. The statistic of the results was performed by the statistical package for the social (SPSS) version 23.0. To compare the treatments, one-way analysis of variance (ANOVA) was performed, followed by the Tukey post hoc test. The results demonstrated a presence of WA in the aqueous extract of Brazilian Vassobia breviflora, as well as its antioxidant potential. The MTT assay in the B16F10 cell line showed cytotoxic ability of treatment with the aqueous extract of Vassobia breviflora, when compared to the controls. In the in vivo model the survival of the animals treated with the combination between the aqueous extract in the highest concentration and the chemotherapeutic Temozolomide was better in relation to the other treatments, compared to the control. The weight and volume of the tumors in the different treatments used did not vary significantly in relation to the control group. It is concluded that from the in vitro results, the plant species studied has a cytotoxic potential. However, for the in vivo assays, it is believed that the aqueous extract does not have a significant potential in the control of melanoma development, requiring models with the use of the ethanolic extract and other fractions of the constituents of the vegetal species for the best evaluation. As well as the use of melanoma induction by other routes of inoculation. Ex vivo tests will be needed to better elucidate the activity of the plant against this model of melanoma.
O câncer corresponde a segunda maior causa de mortes no mundo, atrás apenas das doenças cardiovasculares. Essa patologia faz com que as células adquiram a capacidade de driblar os mecanismos de supressão proliferativa, crescendo indefinidamente e sobrevivendo em ambientes hostis. O melanoma é um tipo de tumor maligno, apresenta alto potencial metastático e pode ser resistente a agentes anticancerígenos convencionais. Apesar de não corresponder ao tipo mais frequente de neoplasia, ele representa alta taxa de mortalidade, devido a sua capacidade de metástases e as dificuldades no tratamento. Por esses motivos, a busca por terapias preventivas e/ou adjuvantes é bastante incentivada. Muitos dos medicamentos anticancerígenos tiveram a sua origem a partir de produtos naturais, semissintéticos e sintéticos. No Brasil, devido a riqueza da flora nacional e o pouco conhecimento sobre os produtos bioativos da mesma, os projetos que buscam esse conhecimento são importantes. O objetivo do presente estudo foi o de verificar a atividade antitumoral in vitro e in vivo de uma planta da família Solanaceae, presente no Rio Grande do Sul. Vassobia breviflora é conhecida pelo seu potencial antitumoral, devido principalmente à presença de vitanolídeos, sendo o principal deles a Vitaferina A (VA). No entanto, os estudos encontrados com a planta brasileira são escassos. A espécie vegetal utilizada no trabalho foi extraída de uma propriedade privada na região de Boca do Monte, no município de Santa Maria, RS. Ela foi catalogada pelo herbário da Universidade Federal de Santa Maria e o seu extrato aquoso foi preparado com as folhas secas, extraídas a temperatura ambiente. Os controles foram feitos com a utilização do princípio ativo VA, adquirido comercialmente e do quimioterápico padrão para o melanoma, a Temozolomida. A verificação da quantidade de VA foi conduzida por cromatografia líquida de alta eficiência (CLAE). Os ensaios para a detecção de compostos antioxidantes no extrato foram feitos a partir da análise de fenóis totais, sal de diamônio 2,2'-Azino-bis (3-etilbenzotiazolina-6-sulfônico) (ABTS) e 2,2-Difenil-1- Picrilidrazilo (DPPH). O potencial citotóxico foi investigado a partir do ensaio de (3 (4,5-dimetiltiazol-2-il)-2,5-difeniltetrazólio brometo) (MTT), em células B16F10. Os testes in vivo foram conduzidos na linhagem C57BL/6, de camundongos isogênicos, inoculados pela via subcutânea, com a linhagem de melanoma B16F10 e tratados por um período de 30 dias, por via oral, com os diferentes tratamentos. O projeto teve aprovação do comitê de ética no uso de animais da Instituição. Durante o período de tratamento foram verificados os pesos dos animais, semanalmente, com o auxílio de balança analítica. O volume do tumor, pela medida do diâmetro da massa tumoral, com paquímetro digital e o tempo de sobrevida pela curva de Kaplan Meyer. Após a eutanásia dos animais, eles tiveram os tumores removidos e pesados. Além disso, outros órgãos como baço, linfonodo, fígado, pulmão, cérebro e rim foram retirados e fixados para posterior análise histopatológica, e devidamente acondicionados e congelados para as análises bioquímica e de expressão gênica. A estatística dos resultados foi realizada pelo statistical package for the social (SPSS) versão 23.0. Para comparar os tratamentos, foram utilizadas análises de variância (ANOVA) de uma via, seguido do teste post hoc de Tukey. Os resultados demonstraram a presença da VA no extrato aquoso da Vassobia breviflora brasileira, assim como o seu potencial antioxidante. O ensaio de MTT na linhagem celular B16F10 mostrou capacidade citotóxica do tratamento com o extrato aquoso de Vassobia breviflora, quando comparado aos controles. No modelo in vivo a sobrevida dos animais tratados com a associação entre o extrato aquoso na maior concentração e o quimioterápico Temozolomida foi melhor em relação aos demais tratamentos, comparados ao controle. O peso e o volume dos tumores, nos diferentes tratamentos utilizados, não variou significativamente em relação ao grupo controle. Conclui-se a partir dos resultados in vitro, que a espécie vegetal estudada apresenta um potencial citotóxico. No entanto, para os ensaios in vivo acredita-se que o extrato aquoso não tenha um potencial significativo no controle do desenvolvimento do melanoma, necessitando de modelos com a utilização do extrato etanólico e demais frações dos constituintes da espécie vegetal para a melhor avaliação. Bem como a utilização de indução do melanoma por outras vias de inoculação. Testes ex vivo serão necessários para a melhor elucidação da atividade da planta frente a esse modelo de melanoma.

وصف الملف: application/pdf

الإتاحة: http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/608Test

المؤلفون: Londero, James Eduardo Lago, Viana, Altevir Rossato, Silva, Larissa Duailibe, Schavinski, Cassiano Ricardo, Schuch, André Passaglia

المساهمون: CAPES, CNPq

المصدر: Science of The Total Environment ; volume 924, page 171647 ; ISSN 0048-9697

مصطلحات موضوعية: Pollution, Waste Management and Disposal, Environmental Chemistry, Environmental Engineering

الإتاحة: https://doi.org/10.1016/j.scitotenv.2024.171647Test
https://api.elsevier.com/content/article/PII:S0048969724017893?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0048969724017893?httpAccept=text/plainTest

المؤلفون: Nunes, Franciane Batista, da Silva Bruckmann, Franciele, Viana, Altevir Rossato, da Rosa Salles, Theodoro, Zancanaro, Leonardo Vidal, Rhoden, Daniele Soares Basso, Franco, Camila, Schuch, André Passaglia, Dotto, Guilherme Luiz, Silva, Luis Felipe Oliveira, Ramos, Claudete Gindri, Rhoden, Cristiano Rodrigo Bohn

المصدر: Journal of Environmental Chemical Engineering ; volume 12, issue 2, page 112336 ; ISSN 2213-3437

مصطلحات موضوعية: Process Chemistry and Technology, Pollution, Waste Management and Disposal, Chemical Engineering (miscellaneous)

الإتاحة: https://doi.org/10.1016/j.jece.2024.112336Test
https://api.elsevier.com/content/article/PII:S2213343724004664?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2213343724004664?httpAccept=text/plainTest

المؤلفون: da Silva, Jean Lucas Gutknecht, Viana, Altevir Rossato, Passos, Daniela Ferreira, Krause, Luciana Maria Fontanari, Miron, Vanessa Valéria, Schetinger, Maria Rosa Chitolina, Pillat, Micheli Mainardi, Palma, Taís Vidal, Leal, Daniela Bitencourt Rosa

المصدر: Purinergic Signalling; Dec2023, Vol. 19 Issue 4, p633-650, 18p

مستخلص: ATP and adenosine exert pivotal roles in the development, maintenance, and metastatic spreading of melanoma. The action of such key melanoma tumor microenvironment (TME) constituents might be complementary or opposed, and their effects are not exclusive to immune cells but also to other host cells and tumor cells. The effects of ATP are controlled by the axis CD39/73, resulting in adenosine, the main actor in the TME, and A2A is the crucial mediator of its effects. We evaluated ATP and adenosine signaling through A2A on B16F10 melanoma cells using istradefylline (IST) (antiparkinsonian A2A antagonist) and caffeine (CAF) treatments after exposure to ATP and adenosine. Adenosine increased melanoma cell viability and proliferation in a concentration-dependent manner. ATP increases viability only as a substrate by CD39 to produce adenosine. Both IST and CAF are toxic to B16F10 cells, but only IST potentialized paclitaxel-induced cytotoxic effects, even decreasing its IC50 value. IST positively modulated CD39 and CD73 expression. CD39 activity was increased, and E-ADA was reduced, indicating that the melanoma cells promoted compensatory feedback in the production and maintenance of adenosine levels. A2A antagonism by IST reduced the factors associated with malignancy, like migration, adhesion, colony formation, and the capacity to produce melanin. Moreover, IST significantly increases nitric oxide (NO) production, which correlates to a decline in melanoma cell viability by apoptotic events. Altogether, our results suggest that adenosine signaling through A2A is essential for B16F10 cells, and its inhibition by IST causes compensatory purinergic enzymatic modulations. Furthermore, IST is a promising therapy that provides new ways to improve current melanoma treatments. [ABSTRACT FROM AUTHOR]

: Copyright of Purinergic Signalling is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Oliveira, Évelin Cogo de, Viana, Altevir Rossato, Serafin, Marissa Bolson, Pompeu, Lenise Deon, Manfron, Melania Palermo

المصدر: Research, Society and Development; Vol. 10 No. 16; e247101623778 ; Research, Society and Development; Vol. 10 Núm. 16; e247101623778 ; Research, Society and Development; v. 10 n. 16; e247101623778 ; 2525-3409

مصطلحات موضوعية: Glechon spathulata, Lamiaceae, Minimum Inhibitory Concentration, Gram positive and gram negative bactéria, Concentración mínima inhibitoria, Bacterias gram positivas y gram negativas, Concentração Inibitória Mínima, Bactérias gram positivas e gram negativas

الوصف: The medicinal species Glechon spathulata Benth, belonging to the family Lamiaceae, known as the "Mangeroninha-do-campo". The objective of the study was to evaluate the antimicrobial activity of the crude extract and fractions obtained in open - column chromatography of G. spathulata through the broth microdilution method, to test against different gram positive and gram-negative bacteria. According to the results obtained, the greatest inhibitory potential occurred against gram negative bacteria. The fractions C and E, G fraction and crude extract presented better results against gram negative bacteria, and the highest inhibitory potential occurred in relation to the two strains of Escherichia coli (ATCC 35218 and ATCC 25922). The crude extract and C and F fractions showed a greater inhibitory effect against Salmonella tiphymurium ATCC 52117. There was also an inhibitory potential against Staphylococcus aureus strains ATCC 25923, both for the crude extract, as well as fractions C and B And fractions F and G. Inhibition of Staphylococcus epidermidis strains ATCC 12228 occurred only in crude extract and ethyl acetate fractions. Both the crude extract and the fractions of G. spathulata presented a potential antibacterial activity. The extract and the fractions presented better activity against the strain of Salmonella tiphymurium when compared to studies that used the same plant and plants of the same family. This study showed that both the crude extract and some fractions of Glechon spathulata Benth are potential candidates for the development of new antimicrobial agents. ; La especie medicinal Glechon spathulata Benth perteneciente a la familia Lamiaceae, conocida como “Manjeroninha-do-campo”. El objetivo del estudio fue evaluar la actividad antimicrobiana del extracto crudo y fracciones obtenidas por cromatografía en columna abierta) de G. spathulata mediante el método de microdilución en caldo, para contrastar diferentes bacterias gram positivas y gram negativas. Según los resultados obtenidos, el mayor ...

وصف الملف: application/pdf

العلاقة: https://rsdjournal.org/index.php/rsd/article/view/23778/20905Test; https://rsdjournal.org/index.php/rsd/article/view/23778Test

الإتاحة: https://doi.org/10.33448/rsd-v10i16.23778Test
https://rsdjournal.org/index.php/rsd/article/view/23778Test

المؤلفون: Viana, Altevir Rossato, Bottega, Angelita, Serafin, Marissa Bolson, Salles, Bruno, Horner, Rosmari, Krause, Alexandre, Maria Fontanari Krause, Luciana, Mortari, Sergio Roberto

المصدر: Research, Society and Development; Vol. 10 No. 11; e391101119755 ; Research, Society and Development; Vol. 10 Núm. 11; e391101119755 ; Research, Society and Development; v. 10 n. 11; e391101119755 ; 2525-3409

مصطلحات موضوعية: Bacteria, Cáncer, Linaje celular, Nanotecnología, Reposicionamiento, Cancer, Linhagem Celular, Nanotecnologia, Reposicionamento, Cell Line, Nanotechnology, Repositioning

الوصف: Antimonials are used as chemotherapy for leishmaniasis, but have limited results due to their toxicity and broad resistance already acquired by the parasites. Nanotechnology offers an alternative to reduce these effects through the use of biocompatible nanocarriers, which can be vectorized to the target site. In addition, the redirection of molecules, already developed for the treatment of other pathologies, has the advantage of being already approved for therapy by regulatory agencies. The present study addresses the production of liposomal vesicles containing antimony trioxide (LC Sb2O3), as well as the evaluation of activity against tumor and bacterial cells. We produce liposomes in order of nanometric size, polydispersity index (PDI <0.3), pH value close to physiological (7.2), and zeta potential (anionic). Cytotoxicity was evaluated in 24 and 72 hours, in the HepG2, T98G, and U87MG tumor cell lines, by the method (3-4.5 dimethylthiazole-2.5 diphenyltetrazolium bromide) (MTT). The minimum inhibitory concentration (MIC) was tested on three bacterial strains (American Type Culture Collection – ATCC-Escherichia coli ATCC 35218, Staphylococcus aureus ATCC 29213 and Enterococcus faecalis ATCC 29212) and mandatory (Staphylococcus aureus and Klebsiella pneumoniae). The liposomes were more cytotoxic than Sb2O3 in the free form, for all tested cell lines. This effect was stronger after 72 hours incubation. Antimony trioxide in both free and liposomal forms showed low antibacterial activity. Based on our results, we suggest that liposomes containing antimony trioxide have the potential for the repositioning of drugs addressing anticancer therapy. ; Los antimoniales se utilizan como quimioterapia para la leishmaniasis, pero tienen resultados limitados debido a su toxicidad y amplia resistencia ya adquirida por los parásitos. La nanotecnología ofrece una alternativa para reducir estos efectos mediante el uso de nanoportadores biocompatibles, que pueden ser vectorizados. Además, el redireccionamiento de moléculas, ya ...

وصف الملف: application/pdf

العلاقة: https://rsdjournal.org/index.php/rsd/article/view/19755/17649Test; https://rsdjournal.org/index.php/rsd/article/view/19755Test

الإتاحة: https://doi.org/10.33448/rsd-v10i11.19755Test
https://rsdjournal.org/index.php/rsd/article/view/19755Test

المؤلفون: Garzon, Litiérri Razia, Fracasso, Mateus, Viana, Altevir Rossato, Giacometi, Marjorie, Samoel, Gisele Vaz Aguirre, Souza, Lucas, Baldissera, Matheus Dellaméa, Petry, Letícia dos Santos, Krause, Luciana Maria Fontanari, Monteiro, Silvia Gonzalez

المصدر: Brazilian Journal of Development; v. 7, n. 8 (2021); 82837-82858 ; 2525-8761

مصطلحات موضوعية: Díptera, Bioterapia, Calliphoridae, Trypanosomatidae Células Tumorais

الوصف: As pesquisas com produtos naturais representam um valioso recurso, pois são fonte de compostos biologicamente ativos que podem ter atividade contra uma variedade de patógenos que afetam a saúde humana e animal. Os dípteros da espécie Lucilia cuprina, pertencente à família Calliphoridae, conhecidos como moscas varejeiras, são cosmopolitas e têm importante função na natureza devido ao desenvolvimento de suas larvas em matéria orgânica em decomposição. Alguns estudos chegaram à conclusão de que a produção de enzimas digestivas secretadas pelas larvas são importantes para o estabelecimento e sobrevivência desses insetos no hospedeiro e no ambiente devido a sua secreção ter ação bactericida, antiparasitária e antifúngica e ao hábito alimentar necrobiontófago, esses dipteros têm sido utilizados em bioterapia e pesquisas contra vários patógenos, entretanto, são poucos os estudos com foco na secreção desta espécie. Portanto, o objetivo deste trabalho foi verificar a atividade da secreção larval in vitro de L. cuprina, extraída de larvas L3 em diferentes concentrações por um período de 24 horas, frente à Leishmania amazonensis, Trypanosoma cruzi, linhagem tumoral de melanoma B16-F10 e HFF-1 de fibroblasto humano. Evidenciou-se uma redução na viabilidade de promastigotas de L. amazonensis nos tratamentos com secreção larval de 0,25%, 1% e 2% (viabilidade reduzida para 43,33%, 60% e 37,33% respectivamente). No ensaio com T. cruzi as concentrações 0,25% e 0,5% de secreção larval foi ativa mostrando diminuição de viabilidade de epimastigotas para 35,28% e 43,07%. A secreção larval mostrou capacidade citotóxica para as células tumorais. A concentração de 2% foi significativa em todos os ensaios, MTT, Vermelho Neutro (NR) e Crystal Violeta (VC), com redução de viabilidade para 79,3%, 85% e 85,6%, respectivamente. O tratamento a 1% mostrou diferença significativa tanto para NR (viabilidade de 74%) como VC (viabilidade de 83,6%). A concentração de 0,5% no NR diminuiu viabilidade para 80,6%. Nos ensaios com linhagem não tumoral a ...

وصف الملف: application/pdf

العلاقة: https://www.brazilianjournals.com/index.php/BRJD/article/view/34759/pdfTest; https://www.brazilianjournals.com/index.php/BRJD/article/view/34759Test

الإتاحة: https://doi.org/10.34117/bjdv7n8-475Test
https://www.brazilianjournals.com/index.php/BRJD/article/view/34759Test

المؤلفون: Franco, Camila, Silva, Manoela Lunkes, Viana, Altevir Rossato, Ourique, Aline Ferreira, Krause, Luciana Maria Fontanari

المصدر: Brazilian Journal of Development; v. 7, n. 7 (2021); 67004-67014 ; 2525-8761

مصطلحات موضوعية: Nanocarreador, Câncer de mama e Viabilidade celular

الوصف: Novas pesquisas indicam que anti-inflamatórios podem ser aplicados como agentes anti-cancerígenos como indometacina para hepatocarcinoma humano, canceres de colon e estômago. Como sabe-se, indometacina possui efeitos adversos gastrointestinais, cardiovasculares e renais. Uma vez que cancer de mama tem alta incidência e não há estudo da indometacina carreada em nanopartículas para esta aplicação, este estudo envolve o desenvolvimento de nanocapsulas de poli-epsilon-caprolactona carregadas com indometacina para a redução de citotoxicidade como agente quimioprotetor para cancer de mama. O nanocarreador foi preparado por método de deposição interfacial e sua caracterização foi realizada por determinação de pH, diâmetro médio e índice de polidispersão por espalhamento dinâmico de luz, potencial zeta por mobilidade eletroforética, eficiência de encapsulação por método de cromatografia líquida de alta eficiência e seu ensaio de citotoxicidade com linhagem de células queratinócitos (HaCaT) e células de cancer de mama (MCF-7). As formulações branca (C-NC) e contendo indometacina (Ind-OH-NC) mostraram leve pH ácido, diâmetros em torno de 200 nm e PDI0,2 com potencial zeta em torno de -20 mV e eficiência de encapsulação de 99% (1 mg.mL-1), cujo coeficiente de distribuição indicou efeito de permeação e retenção (efeito EPR). Ambas formulações não foram citotóxicas às células HaCaT, provando serem seguras às células normais e Ind-OH-NC teve uma permeação concentração e tempo-dependente e teve eficácia em reduzir a viabilidade celular da linhagem MCF-7.

وصف الملف: application/pdf

العلاقة: https://www.brazilianjournals.com/index.php/BRJD/article/view/32500/pdfTest; https://www.brazilianjournals.com/index.php/BRJD/article/view/32500Test

الإتاحة: https://doi.org/10.34117/bjdv7n7-124Test
https://www.brazilianjournals.com/index.php/BRJD/article/view/32500Test

المؤلفون: Chuy, Gabriela Pereira, Muraro, Pâmela Cristine Ladwig, Viana, Altevir Rossato, Pavoski, Giovani, Espinosa, Denise Crocce Romano, Vizzotto, Bruno Stefanello, da Silva, William Leonardo

المساهمون: Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul

المصدر: Journal of Inorganic and Organometallic Polymers and Materials ; volume 32, issue 4, page 1213-1222 ; ISSN 1574-1443 1574-1451

مصطلحات موضوعية: Materials Chemistry, Polymers and Plastics

الإتاحة: https://doi.org/10.1007/s10904-021-02162-3Test

المؤلفون: Jantsch, Matheus Henrique, Doleski, Pedro Henrique, Viana, Altevir Rossato, da Silva, Jean Lucas Gutknecht, Passos, Daniela Ferreira, Cabral, Fernanda Licker, Manzoni, Alessandra Guedes, Ebone, Renan da Silva, Soares, Ana Bárbara Uchoa, de Andrade, Cínthia Melazzo, Schetinger, Maria Rosa Chitolina, Leal, Daniela Bitencourt Rosa

المصدر: Biochemistry & Cell Biology; Oct2023, Vol. 101 Issue 5, p443-455, 13p

مصطلحات موضوعية: CLOPIDOGREL, ROOT-tubercles, MELANOMA, ADENOSINE diphosphate, BLOOD platelet activation, LACTATE dehydrogenase, BLOOD platelet aggregation, CORD blood

مستخلص: Metastatic melanoma is a very aggressive skin cancer. Platelets are constituents of the tumor microenvironment and, when activated, contribute to cancer progression, especially metastasis and inflammation. P2Y12 is an adenosine diphosphate receptor that triggers platelet activation. Inhibition of P2Y12 by clopidogrel bisulfate (CB) decreases platelet activation, which is also controlled by the extracellular concentration and the metabolism of purines by purinergic enzymes. We evaluated the effects of CB on the viability and proliferation of cultured B16-F10 cells. We also used a metastatic melanoma model with C57BL-6 mice to evaluate cancer development and purine metabolism modulation in platelets. B16-F10 cells were administered intraperitoneally to the mice. Two days later, the animals underwent a 12-day treatment with CB (30 mg/kg by gavage). We have found that CB reduced cell viability and proliferation in B16-F10 culture in 72 h at concentrations above 30 µm. In vivo, CB decreased tumor nodule counts and lactate dehydrogenase levels and increased platelet purine metabolism. Our results showed that CB has significant effects on melanoma progression. [ABSTRACT FROM AUTHOR]

: Copyright of Biochemistry & Cell Biology is the property of Canadian Science Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)