المؤلفون: Ruiz-López, María José, Muñoz-Chimeno, Milagros, Figuerola, Jordi, Gavilán, Ana M., Varona, Sarai, Cuesta, Isabel, Martínez de la Puente, Josué, Zaballos, Ángel, Molero, Francisca, Soriguer, Ramón, Sánchez-Seco, Maria Paz, Ruiz, Santiago, Vázquez, Ana

مصطلحات موضوعية: Culex, Complete genome, Flavivirus, Vector-borne diseases, Zoonosis

الوصف: Emerging infectious diseases are one of the most important global health challenges because of their impact on human and animal health. The vector-borne West Nile virus (WNV) is transmitted between birds by mosquitos, but it can also infect humans and horses causing disease. The local circulation of WNV in Spain has been known for decades, and since 2010, there have been regular outbreaks in horses, although only six cases were reported in humans until 2019. In 2020, Spain experienced a major outbreak with 77 human cases, which was followed by 6 additional cases in 2021, most of them in the Andalusian region (southern Spain). This study aimed to characterize the genomes of the WNV circulating in wild-trapped mosquitoes during 2020 and 2021 in Andalusia. We sequenced the WNV consensus genome from two mosquito pools and carried out the phylogenetic analyses. We also compared the obtained genomes with those sequenced from human samples obtained during the outbreak and the genomes obtained previously in Spain from birds (2007 and 2017), mosquitoes (2008) and horses (2010) to better understand the eco-epidemiology of WNV in Spain. As expected, the WNV genomes recovered from mosquito pools in 2020 were closely related to those recovered from humans of the same outbreak. In addition, the strain of WNV circulating in 2021 was highly related to the WNV strain that caused the 2020 outbreak, suggesting that WNV is overwintering in the area. Consequently, future outbreaks of the same strain may occur in in the future. ; This research was funded by the Research State Agency projects PGC2018-095704-B-I00 and PID2020-118921RJ-I00 ; Instituto de Salud Carlos III Project PI19CIII_00014 ; European Commission—NextGenerationEU (Regulation EU 2020/2094), through CSIC’s Global Health Platform (PTI Salud Global+).

العلاقة: Ruiz-López, M.J.; Muñoz-Chimeno, M.; Figuerola, J.; Gavilán, A.M.; Varona, S.; Cuesta, I.; Martínez-de la Puente, J.; Zaballos, Á.; Molero, F.; Soriguer, R.C.; Sánchez-Seco, M.P.; Ruiz, S.; Vázquez, A. Genomic Analysis of West Nile Virus Lineage 1 Detected in Mosquitoes during the 2020–2021 Outbreaks in Andalusia, Spain. Viruses 2023, 15, 266. https://doi.org/10.3390/v15020266Test; https://hdl.handle.net/10481/79073Test

الإتاحة: https://doi.org/10.3390/v15020266Test
https://hdl.handle.net/10481/79073Test

المؤلفون: Pérez Luz, Sara, Lalchandani, Jaanam, Matamala, Nerea, Barrero, Maria Jose, Gil-Marín, Sara, Ramos-Del Saz, Sheila, Varona, Sarai, Monzón, Sara, Cuesta, Isabel, Justo, Iago, Marcacuzco, Alberto, Hierro, Loreto, Garfia, Cristina, Gomez-Mariano, Gema, Janciauskiene, Sabina, Martínez-Delgado, Beatriz

مصطلحات موضوعية: 572.1/.4, Alpha-1 antitrypsin deficiency (AATD), Alpha-1 antitrypsin (AAT), Liver organoids, AAT aggregates, Lipid accumulation, Ciencias Biomédicas, 2410 Biología Humana

الوصف: Author Contributions: S.P.-L., J.L., N.M., S.R.-D.S. and G.G.-M. isolated the organoids; S.P.-L., J.L., S.G.-M. and G.G.-M. performed the experiments; S.V., S.M. and I.C. analyzed data; I.J., A.M., L.H. and C.G. provided liver biopsies; S.P.-L. and G.G.-M. wrote the manuscript; M.J.B. and S.J. analyzed data and contributed to critical data interpretation; B.M.-D. conceived and supervised the study. All authors have provided comments on the manuscript. All authors have read and agreed to the published version of the manuscript. ; Different mutations in the SERPINA1 gene result in alpha-1 antitrypsin (AAT) deficiency and in an increased risk for the development of liver diseases. More than 90% of severe deficiency patients are homozygous for Z (Glu342Lys) mutation. This mutation causes Z-AAT polymerization and intrahepatic accumulation which can result in hepatic alterations leading to steatosis, fibrosis, cirrhosis, and/or hepatocarcinoma. We aimed to investigate lipid status in hepatocytes carrying Z and normal M alleles of the SERPINA1 gene. Hepatic organoids were developed to investigate lipid alterations. Lipid accumulation in HepG2 cells overexpressing Z-AAT, as well as in patientderived hepatic organoids from Pi*MZ and Pi*ZZ individuals, was evaluated by Oil-Red staining in comparison to HepG2 cells expressing M-AAT and liver organoids from Pi*MM controls. Furthermore, mass spectrometry-based lipidomics analysis and transcriptomic profiling were assessed in Pi*MZ and Pi*ZZ organoids. HepG2 cells expressing Z-AAT and liver organoids from Pi*MZ and Pi*ZZ patients showed intracellular accumulation of AAT and high numbers of lipid droplets. These latter paralleled with augmented intrahepatic lipids, and in particular altered proportion of triglycerides, cholesterol esters, and cardiolipins. According to transcriptomic analysis, Pi*ZZ organoids possess many alterations in genes and cellular processes of lipid metabolism with a specific impact on the endoplasmic reticulum, mitochondria, and peroxisome ...

وصف الملف: application/pdf

العلاقة: https://hdl.handle.net/20.500.14352/102052Test; Pérez-Luz, S.; Lalchandani, J.; Matamala, N.; Barrero, M.J.; Gil-Martín, S.; Saz, S.R.-D.; Varona, S.; Monzón, S.; Cuesta, I.; Justo, I.; et al. Quantitative Lipid Profiling Reveals Major Differences between Liver Organoids with Normal Pi*M and Deficient Pi*Z Variants of Alpha-1-antitrypsin. Int. J. Mol. Sci. 2023, 24, 12472. https://doi.orgTest/ 10.3390/ijms241512472

الإتاحة: https://doi.org/20.500.14352/102052Test
https://hdl.handle.net/20.500.14352/102052Test

المؤلفون: Ruiz-López, María José, Aguilera-Sepúlveda, Pilar, Cebrián-Camisón, Sonia, Figuerola, Jordi, Magallanes, Sergio, Varona, Sarai, Cuesta, Isabel, Cano-Gómez, Cristina, Sánchez-Mora, Patricia, Camacho, Juan, Sánchez-Peña, Carolina, Marchena, Francisco José, Ameyugo, Ulises, Ruíz, Santiago, Sánchez-Seco, María Paz, Agüero, Montserrat, Jiménez-Clavero, Miguel Ángel, Fernández-Pinero, Jovita, Vázquez, Ana

الوصف: West Nile Virus (WNV) is a mosquito vector-borne zoonosis with an increasing incidence in Europe that has become a public health concern. In Spain, although local circulation has been known for decades, until 2020, when a large outbreak occurred, West Nile Virus cases were scarce and mostly occurred in southern Spain. Since then, there have been new cases every year and the pathogen has spread to new regions. Thus, monitoring of circulating variants and lineages plays a fundamental role in understanding WNV evolution, spread and dynamics. In this study, we sequenced WNV consensus genomes from mosquito pools captured in 2022 as part of a newly implemented surveillance program in southern Spain and compared it to other European, African and Spanish sequences. Characterization of WNV genomes in mosquitoes captured in 2022 reveals the co-circulation of two WNV lineage 1 variants, the one that caused the outbreak in 2020 and another variant that is closely related to variants reported in Spain in 2012, France in 2015, Italy in 2021–2022 and Senegal in 2012–2018. The geographic distribution of these variants indicates that WNV L1 dynamics in southern Europe include an alternating dominance of variants in some territories.

العلاقة: Viruses 15 (12): 2372 (2023); http://hdl.handle.net/10261/340208Test

الإتاحة: https://doi.org/10.3390/v15122372Test
http://hdl.handle.net/10261/340208Test

المؤلفون: Monzón, Sara, Varona, Sarai, Negredo, Anabel, Vidal-Freire, Santiago, Patiño-Galindo, Juan Angel, Ferressini-Gerpe, Natalia, Zaballos, Angel, Orviz, Eva, Ayerdi, Oskar, Muñoz-Gómez, Ana, Delgado-Iribarren, Alberto, Estrada, Vicente, García, Cristina, Molero, Francisca, Sánchez-Mora, Patricia, Torres, Montserrat, Vázquez, Ana, Galán, Juan-Carlos, Torres, Ignacio, Causse del Río, Manuel

المصدر: Nature Communications; 4/18/2024, Vol. 15 Issue 1, p1-18, 18p

مصطلحات موضوعية: MONKEYPOX, MICROSATELLITE repeats, SHORT tandem repeat analysis, SINGLE nucleotide polymorphisms

مصطلحات جغرافية: NIGERIA

مستخلص: The 2023 monkeypox (mpox) epidemic was caused by a subclade IIb descendant of a monkeypox virus (MPXV) lineage traced back to Nigeria in 1971. Person-to-person transmission appears higher than for clade I or subclade IIa MPXV, possibly caused by genomic changes in subclade IIb MPXV. Key genomic changes could occur in the genome's low-complexity regions (LCRs), which are challenging to sequence and are often dismissed as uninformative. Here, using a combination of highly sensitive techniques, we determine a high-quality MPXV genome sequence of a representative of the current epidemic with LCRs resolved at unprecedented accuracy. This reveals significant variation in short tandem repeats within LCRs. We demonstrate that LCR entropy in the MPXV genome is significantly higher than that of single-nucleotide polymorphisms (SNPs) and that LCRs are not randomly distributed. In silico analyses indicate that expression, translation, stability, or function of MPXV orthologous poxvirus genes (OPGs), including OPG153, OPG204, and OPG208, could be affected in a manner consistent with the established "genomic accordion" evolutionary strategies of orthopoxviruses. We posit that genomic studies focusing on phenotypic MPXV differences should consider LCR variability. The 2023 monkeypox outbreak was caused by a subclade IIb monkeypox virus (MPXV). Here, using advanced sequencing techniques, the authors identify variations on low-complexity regions of the MPXV genome and describe their potential as evolutionary drivers. [ABSTRACT FROM AUTHOR]

: Copyright of Nature Communications is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Garcia Vidal, Carolina, Iglesias, María, Puerta Alcalde, Pedro, Mas, Vicente, Cuesta Chasco, Genoveva, García Pouton, Nicole, Varona, Sarai, Pozo, Francisco, Vázquez Morón, Sonia, Marcos, Ma. Angeles, Soriano Viladomiu, Alex, Casas Flecha, Inmaculada, HEMATOCOVID19-Researchers Group

المصدر: Articles publicats en revistes (Fonaments Clínics)

مصطلحات موضوعية: COVID-19, SARS-CoV-2, Medicaments antivírics, Hematologia, Immunosupressió, Mutació (Biologia), Antiviral agents, Hematology, Immunosuppression, Mutation (Biology)

الوصف: We documented a hematologic patient with prolonged SARS-CoV-2 viral replication in whom emergence of viral mutations was documented after the consecutive use of antivirals and convalescent plasma. The virus detected in the last of 12 clinical samples (day 237) had accumulated 22 changes in amino acids and 29 in nucleotides. Some of these changes, such as the E484Q, were mutations of concern as defined by WHO. This finding represents an enormous epidemiological threat and poses a major clinical challenge. Combined antiviral strategies, as well as specific strategies related to the diagnostic approach of prolonged infections for this specific population, may be needed.

وصف الملف: 15 p.; application/pdf

العلاقة: Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2022.826883Test; Frontiers in Microbiology, 2022, vol. 13, p. 826883; https://doi.org/10.3389/fmicb.2022.826883Test; http://hdl.handle.net/2445/197930Test; 730209; 9300893

الإتاحة: https://doi.org/10.3389/fmicb.2022.826883Test
http://hdl.handle.net/2445/197930Test

المؤلفون: Garcia-Vidal, Carolina, Iglesias-Caballero, María, Puerta-Alcalde, Pedro, Mas, Vicente, Cuesta-Chasco, Genoveva, Garcia-Pouton, Nicole, Varona, Sarai, Pozo, Francisco, Vázquez-Morón, Sonia, Marcos, Maria Angeles, Soriano, Alex, Casas, Inmaculada

المساهمون: Merck Sharp and Dohme, Pfizer

المصدر: Frontiers in Microbiology ; volume 13 ; ISSN 1664-302X

مصطلحات موضوعية: Microbiology (medical), Microbiology

الوصف: We documented a hematologic patient with prolonged SARS-CoV-2 viral replication in whom emergence of viral mutations was documented after the consecutive use of antivirals and convalescent plasma. The virus detected in the last of 12 clinical samples (day 237) had accumulated 22 changes in amino acids and 29 in nucleotides. Some of these changes, such as the E484Q, were mutations of concern as defined by WHO. This finding represents an enormous epidemiological threat and poses a major clinical challenge. Combined antiviral strategies, as well as specific strategies related to the diagnostic approach of prolonged infections for this specific population, may be needed.

الإتاحة: https://doi.org/10.3389/fmicb.2022.826883Test

المؤلفون: Iglesias-Caballero, María, Camarero-Serrano, Sara, Varona, Sarai, Mas, Vicente, Calvo, Cristina, García, María Luz, García-Costa, Juan, Vázquez-Morón, Sonia, Monzón, Sara, Campoy, Albert, Cuesta, Isabel, Pozo, Francisco, Casas, Inmaculada

المصدر: Eurosurveillance (15607917); 12/7/2023, Vol. 28 Issue 49, p5-10, 6p

المؤلفون: Lara-Aguilar, Violeta, Rueda, Cristina, García-Barbazán, Irene, Varona, Sarai, Monzón, Sara, Jiménez, Pilar, Cuesta, Isabel, Zaballos, Ángel, Zaragoza, Óscar

المساهمون: Red Española de Investigación en Patología Infecciosa, Ministerio de Ciencia e Innovacion

المصدر: Virulence ; volume 12, issue 1, page 1400-1417 ; ISSN 2150-5594 2150-5608

الإتاحة: https://doi.org/10.1080/21505594.2021.1927609Test

المؤلفون: Díez-Fuertes, Francisco, Iglesias-Caballero, María, García-Pérez, Javier, Monzón, Sara, Jiménez, Pilar, Varona, Sarai, Cuesta, Isabel, Zaballos, Ángel, Jiménez, Mercedes, Checa, Laura, Pozo, Francisco, Pérez-Olmeda, Mayte, Thomson, Michael M., Alcamí, José, Casas, Inmaculada

المساهمون: Sandri-Goldin, Rozanne M., MEC | Instituto de Salud Carlos III

المصدر: Journal of Virology ; volume 95, issue 3 ; ISSN 0022-538X 1098-5514

الوصف: Multiple SARS-CoV-2 introductions have been detected in Spain, and at least four resulted in the emergence of locally transmitted clusters that originated not later than mid-February, with further dissemination to many other countries around the world, and a few weeks before the explosion of COVID-19 cases detected in Spain during the first week of March. The majority of the earliest variants detected in Spain branched in the clade 19B (D614 viruses), which was the most prevalent clade during the first weeks of March, pointing to a founder effect. However, from mid-March to June 2020, G614-bearing viruses (clades 20A, 20B, and 20C) overcame D614 variants in Spain, probably as a consequence of an evolutionary advantage of this substitution in the spike protein. A higher infectivity of G614-bearing viruses than D614 variants was detected, suggesting that this substitution in SARS-CoV-2 spike protein could be behind the variant shift observed in Spain.

الإتاحة: https://doi.org/10.1128/jvi.01583-20Test

المؤلفون: Zaballos, Ángel, Varona, Sarai, Iglesias Caballero, María, Monzón, Sara, Pozo, Francisco, Casas, Inmaculada, Cuesta, Isabel

المساهمون: Departamentos de la UMH::Producción Vegetal y Microbiología

مصطلحات موضوعية: Genómica, Bioinformática, Análisis filogenético, Epidemiología molecular, CDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiología

الوصف: La pandemia causada por el nuevo coronavirus SARS-CoV-2 no sólo está afectando de manera muy sensible al modo de vida de miles de millones de personas, sino que, a la vez de reto científico y médico, está suponiendo un nuevo paradigma en la detección, caracterización y seguimiento epidemiológico del agente causal. Y sin lugar a duda la secuenciación completa del genoma de SARS-CoV-2 a partir de muestras clínicas es el estandarte de este nuevo paradigma

وصف الملف: application/pdf

العلاقة: 70; http://hdl.handle.net/11000/7330Test

الإتاحة: http://hdl.handle.net/11000/7330Test