المؤلفون: Karageorgou, Maria-Argyro, Apostolopoulou, Adamantia, Tomazinaki, Mina-Ermioni, Stanković, Dragana, Stiliaris, Efstathios, Bouziotis, Penelope, Stamopoulos, Dimosthenis

المصدر: Materials

مصطلحات موضوعية: biocompatibility, cellular distribution, dual-modality contrast agent, gamma-camera imaging, iron oxide nanoparticles, peripheral human blood, technetium-99m

الوصف: The radiolabeled iron oxide nanoparticles constitute an attractive choice to be used as dual-modality contrast agents (DMCAs) in nuclear medical diagnosis, due to their ability to combine the benefits of two imaging modalities, for instance single photon emission computed tomography (SPECT) with magnetic resonance imaging (MRI). Before the use of any DMCA, the investigation of its plasma extra- and on/intra cellular distribution in peripheral human blood is of paramount importance. Here, we focus on the in vitro investigation of the distribution of 99mTc-DPD-Fe3O4 DMCA in donated peripheral human blood (the ligand 2-3-dicarboxypropane-1-1-diphosphonic-acid is denoted as DPD). Initially, we described the experimental methods we performed for the radiosynthesis of the 99mTc-DPD-Fe3O4, the preparation of whole blood and blood plasma samples, and their incubation conditions with 99mTc-DPD-Fe3O4. More importantly, we employed a gamma-camera apparatus for the direct imaging of the 99mTc-DPD-Fe3O4-loaded whole blood and blood plasma samples when subjected to specialized centrifugation protocols. The direct comparison of the gamma-camera data obtained at the exact same samples before and after their centrifugation enabled us to clearly identify the distribution of the 99mTc-DPD-Fe3O4 in the two components, plasma and cells, of peripheral human blood.

العلاقة: https://vinar.vin.bg.ac.rs/handle/123456789/12719Test; 2-s2.0-85183315370; http://vinar.vin.bg.ac.rs/bitstream/id/35100/materials-17-00335.pdfTest

الإتاحة: https://doi.org/10.3390/ma17020335Test
https://vinar.vin.bg.ac.rs/handle/123456789/12719Test
http://vinar.vin.bg.ac.rs/bitstream/id/35100/materials-17-00335.pdfTest

المؤلفون: Karageorgou, Maria-Argyro, Apostolopoulou, Adamantia, Tomazinaki, Mina-Ermioni, Stanković, Dragana, Stiliaris, Efstathios, Bouziotis, Penelope, Stamopoulos, Dimosthenis

المصدر: Materials (1996-1944); Jan2024, Vol. 17 Issue 2, p335, 13p

مصطلحات موضوعية: SINGLE-photon emission computed tomography, IRON oxides, CONTRAST media, IRON oxide nanoparticles, BLOOD plasma, OXYGEN carriers, MAGNETIC resonance imaging

مستخلص: The radiolabeled iron oxide nanoparticles constitute an attractive choice to be used as dual-modality contrast agents (DMCAs) in nuclear medical diagnosis, due to their ability to combine the benefits of two imaging modalities, for instance single photon emission computed tomography (SPECT) with magnetic resonance imaging (MRI). Before the use of any DMCA, the investigation of its plasma extra- and on/intra cellular distribution in peripheral human blood is of paramount importance. Here, we focus on the in vitro investigation of the distribution of ^99mTc-DPD-Fe3O4 DMCA in donated peripheral human blood (the ligand 2-3-dicarboxypropane-1-1-diphosphonic-acid is denoted as DPD). Initially, we described the experimental methods we performed for the radiosynthesis of the ^99mTc-DPD-Fe3O4, the preparation of whole blood and blood plasma samples, and their incubation conditions with ^99mTc-DPD-Fe3O4. More importantly, we employed a gamma-camera apparatus for the direct imaging of the ^99mTc-DPD-Fe3O4-loaded whole blood and blood plasma samples when subjected to specialized centrifugation protocols. The direct comparison of the gamma-camera data obtained at the exact same samples before and after their centrifugation enabled us to clearly identify the distribution of the ^99mTc-DPD-Fe3O4 in the two components, plasma and cells, of peripheral human blood. [ABSTRACT FROM AUTHOR]

: Copyright of Materials (1996-1944) is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Tomazinaki, Mina-Ermioni, Stiliaris, Efstathios

المساهمون: State Scholarships Foundation, European Social Fund, European Commission

المصدر: Computers in Biology and Medicine ; volume 166, page 107502 ; ISSN 0010-4825

مصطلحات موضوعية: Health Informatics, Computer Science Applications

الإتاحة: https://doi.org/10.1016/j.compbiomed.2023.107502Test
https://api.elsevier.com/content/article/PII:S0010482523009678?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0010482523009678?httpAccept=text/plainTest

المؤلفون: Tomazinaki, Mina-Ermioni, Mikeli, Maria, Stiliaris, Efstathios

المساهمون: European Social Fund

المصدر: 2021 IEEE Nuclear Science Symposium and Medical Imaging Conference (NSS/MIC)

الإتاحة: https://doi.org/10.1109/nss/mic44867.2021.9875747Test
http://xplorestaging.ieee.org/ielx7/9875398/9875424/09875747.pdf?arnumber=9875747Test

المؤلفون: Mikeli, Maria, Tomazinaki, Mina-Ermioni, Stiliaris, Efstathios

المصدر: 2021 IEEE Nuclear Science Symposium and Medical Imaging Conference (NSS/MIC)

الإتاحة: https://doi.org/10.1109/nss/mic44867.2021.9875470Test
http://xplorestaging.ieee.org/ielx7/9875398/9875424/09875470.pdf?arnumber=9875470Test

المؤلفون: Tomazinaki, Mina-Ermioni, Mikeli, Maria, Stiliaris, Efstathios

المساهمون: European Social Fund

المصدر: 2021 IEEE Nuclear Science Symposium and Medical Imaging Conference (NSS/MIC)

الإتاحة: https://doi.org/10.1109/nss/mic44867.2021.9875523Test
http://xplorestaging.ieee.org/ielx7/9875398/9875424/09875523.pdf?arnumber=9875523Test

المؤلفون: Mikeli, Maria, Tomazinaki, Mina-Ermioni, Stiliaris, Efstathios

المصدر: 2020 IEEE Nuclear Science Symposium and Medical Imaging Conference (NSS/MIC)

الإتاحة: https://doi.org/10.1109/nss/mic42677.2020.9507802Test
http://xplorestaging.ieee.org/ielx7/9507738/9507739/09507802.pdf?arnumber=9507802Test

المؤلفون: Zarketan, Despoina, Tomazinaki, Mina-Ermioni, Stiliaris, Efstathios

المصدر: 2019 IEEE Nuclear Science Symposium and Medical Imaging Conference (NSS/MIC)

الإتاحة: https://doi.org/10.1109/nss/mic42101.2019.9059771Test
http://xplorestaging.ieee.org/ielx7/9039702/9059608/09059771.pdf?arnumber=9059771Test

المؤلفون: Lytrosyngounis, Ioannis, Zarketan, Despoina, Argyrou, Maria, Maintas, Dimitrios, Mainta, Catherina, Tomazinaki, Mina-Ermioni, Stiliaris, Efstathios

المصدر: 2018 IEEE Nuclear Science Symposium and Medical Imaging Conference Proceedings (NSS/MIC)

الإتاحة: https://doi.org/10.1109/nssmic.2018.8824455Test
http://xplorestaging.ieee.org/ielx7/8821248/8824226/08824455.pdf?arnumber=8824455Test