المؤلفون: Boroumand, M, Manconi, B, Serrao, S, Iavarone, F, Olianas, A, Cabras, T, Contini, C, Pieroni, L, Sanna, MT, Vento, G, Tirone, C, Desiderio, C, Fiorita, A, Faa, G, Messana, I, Castagnola, M

المساهمون: Boroumand, M, Manconi, B, Serrao, S, Iavarone, F, Olianas, A, Cabras, T, Contini, C, Pieroni, L, Sanna, Mt, Vento, G, Tirone, C, Desiderio, C, Fiorita, A, Faa, G, Messana, I, Castagnola, M

مصطلحات موضوعية: Cystatin B, Cysteinylation, Glutathionylation, Preterm human saliva, S100A9

الوصف: Glutathionylation and cysteinylation can be involved in the protection of critical cysteines from irreversible oxidative damages. S100A9 long and cystatin B, proteins highly represented in the saliva of preterm and at-term newborns, can undergo these modifications. Levels of S100A9 long and cystatin B and their glutathionylated and cysteinylated derivatives have been determined by a top-down platform based on high-performance liquid chromatography-electrospray ionization-mass spectrometry in 100 salivary samples serially collected from 17 preterm newborns with different postconceptional age at birth (178-226 days) and in 90 salivary samples collected from at-term newborns and babies. Results showed that: (1) S100A9 long and cystatin B were mainly present as unmodified forms in extremely preterm newborn; (2) the percentage of the S-thiolated derivatives of both proteins increased with increasing the postconceptional age; (3) the greatest variation occurred up to about 280 days of postconceptional age. Interestingly, differences in the levels of the S-thiolated derivatives only depended on the postconceptional age and not on whether the infant was born preterm or at-term. Inadequate levels of cysteine and glutathione might be responsible for the low level of S-thiolated derivatives measured in preterm newborns. Data are available via ProteomeXchange with identifier PXD025517.

العلاقة: info:eu-repo/semantics/altIdentifier/wos/WOS:000729582000001; volume:5; issue:1; firstpage:17; lastpage:27; numberofpages:11; journal:SEPARATION SCIENCE PLUS; https://hdl.handle.net/11584/363804Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85147395421

الإتاحة: https://doi.org/10.1002/sscp.202100049Test
https://hdl.handle.net/11584/363804Test

المؤلفون: Migliazzi, C, Della Costanza, C, Aliprandi, E, Tirone, C, Galassi, M, Ladisa, V

المصدر: Section 5: Patient Safety and Quality assurance

الإتاحة: https://doi.org/10.1136/ejhpharm-2020-eahpconf.362Test

المؤلفون: Tirone C., Iavarone F., Tana M., Lio A., Aurilia C., Costa S., Castagnola M., Messana I., Vento G.

المساهمون: Tirone, Chiara, Iavarone, Federica, Tana, Milena, Lio, Alessandra, Aurilia, Claudia, Costa, Simonetta, Castagnola, Massimo, Messana, Irene, Vento, Giovanni

مصطلحات موضوعية: alpha-1 antitrypsin, bronchoalveolar lavage fluid, bronchopulmonary displasia, preterm infants, proteomics, Settore BIO/10 - BIOCHIMICA

الوصف: The study investigates the role of the oxidative and proteolytic inactivation of alpha-1 antitrypsin (AAT) in the pathogenesis of bronchopulmonary dysplasia (BPD) in premature infants. Bronchoalveolar lavage fluid (BALF) samples were collected on the 3rd day of life from mechanically ventilated neonates with gestational age ≤ 30 weeks and analyzed without previous treatment (top-down proteomics) by reverse-phase high-performance liquid chromatography-electrospray ionization mass spectrometry. AAT fragments were identified by high-resolution LTQ Orbitrap XL experiments and the relative abundances determined by considering the extracted ion current (XIC) peak area. Forty preterm neonates were studied: 20 (50%) did not develop BPD (no-BPD group), 17 (42.5%) developed mild or moderate new-BPD (mild + moderate BPD group), and 3 (7.5%) developed severe new-BPD (severe BPD group). Eighteen fragments of AAT and a fragment of AAT oxidized at a methionine residue were identified: significantly higher values of AAT fragments 25–57, 375–418, 397–418, 144–171, and 397–418 with oxidized methionine were found in the severe BPD group. The significantly higher levels of several AAT fragments and of the fragment 397–418, oxidized in BALF of preterm infants developing BPD, underlie the central role of an imbalance between proteases and protease inhibitors in exacerbating lung injury and inducing most severe forms of BPD. The study has some limitations, and between them, the small sample size implies the need for further confirmation by larger studies.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/33834009; info:eu-repo/semantics/altIdentifier/wos/WOS:000636835200001; volume:9; issue:9; firstpage:597415; lastpage:7; issueyear:2021; journal:FRONTIERS IN PEDIATRICS; https://hdl.handle.net/10807/221929Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85103768933

الإتاحة: https://doi.org/10.3389/fped.2021.597415Test
https://hdl.handle.net/10807/221929Test

المؤلفون: Vento, Giovanni, Ventura, M L, Pastorino, R, van Kaam, A H, Carnielli, V, Cools, F, Dani, C, Mosca, F, Polglase, G, Tagliabue, P, Boni, L, Cota, F, Tana, M, Tirone, C, Aurilia, C, Lio, A, Cost, S, D'Andrea, V, Lucente, M, Nigro, G, Giordano, L, Roma, V, Villani, P, Fusco, F P, Fasolato, V, Colnaghi, M R, Matassa, P G, Vendettuoli, V, Poggi, C, Del Vecchi, A, Petrillo, F, Betta, P, Mattia, C, Garani, G, Solinas, A, Gitto, E, Salvo, V, Gargano, G, Balestri, E, Sandri, F, Mescoli, G, Martinelli, S, Ilardi, L, Ciarmoli, E, Di Fabio, S, Maranella, E, Grassia, C, Ausanio, G, Rossi, V, Motta, A

المصدر: The Lancet Respiratory Medicine ; volume 9, issue 2, page e12 ; ISSN 2213-2600

مصطلحات موضوعية: Pulmonary and Respiratory Medicine

الإتاحة: https://doi.org/10.1016/s2213-2600Test(21)00037-0
https://api.elsevier.com/content/article/PII:S2213260021000370?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2213260021000370?httpAccept=text/plainTest

المؤلفون: Vento G., Tirone C., Iavarone F.

المساهمون: Capasso, L., Vento, G., Loddo, C., Tirone, C., Iavarone, F., Raimondi, F., Dani, C., Fanos, V.

مصطلحات موضوعية: Bronchopulmonary dysplasia, Metabolomics, Microbiomics, Newborn, Oxidative stress, Preterm, Proteomics, Settore MED/38 - PEDIATRIA GENERALE E SPECIALISTICA

الوصف: Bronchopulmonary dysplasia is a major issue affecting morbidity and mortality of surviving premature babies. Preterm newborns are particularly susceptible to oxidative stress and infants with bronchopulmonary dysplasia have a typical oxidation pattern in the early stages of this disease, suggesting the important role of oxidative stress in its pathogenesis. Bronchopulmonary dysplasia is a complex disease where knowledge advances as new investigative tools become available. The explosion of the "omics" disciplines has recently affected BPD research. This review focuses on the new evidence coming from microbiomics, metabolomics and proteomics in relation to oxidative stress and pathogenesis of bronchopulmonary dysplasia. Since the pathogenesis is not yet completely understood, information gained in this regard would be important for planning an efficacious prevention and treatment strategy for the future.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/30815432; info:eu-repo/semantics/altIdentifier/wos/WOS:000458723800001; volume:7; issue:FEB; firstpage:N/A; lastpage:N/A; issueyear:2019; journal:FRONTIERS IN PEDIATRICS; http://hdl.handle.net/10807/164639Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85064413922

الإتاحة: https://doi.org/10.3389/fped.2019.00030Test
http://hdl.handle.net/10807/164639Test

المؤلفون: Tirone, C, Bertoli, S, Galassi, M, Della Costanza, C, Ladisa, V

المصدر: Section 3: Production and Compounding

الإتاحة: https://doi.org/10.1136/ejhpharm-2019-eahpconf.109Test

المؤلفون: Rodrigues T. F., Alves P. U. M., Tirone C., Prade D.

المصدر: Psychology in Russia: State of Art, Vol 9, Iss 4, Pp 163-192 (2016)

مصطلحات موضوعية: autism, contingent emotional development, mediation, zone of proximal development, Psychology, BF1-990

الوصف: Guided by the principle that scientific knowledge should serve to transform reality and create suitable conditions of life for all, the Portuguese Association for Relational-Historical Psychology (APPRH) founded a school named RITA LEAL (RLS), with a therapeutic purpose based on new perspectives for treating autism — perspectives quite different from instrumental and behavioral learning programs. The Rita Leal School (Leal, 1975/2004, 1997, 2005, 2010) is rooted in the theory that mental development is based on a mutually contingent emotional relationship, while it underwrites Vygotsky’s concept of the Zone of Proximal Development (ZPD) and Mediation (1930/2004, 1934/2009). Learning to read is a complex process which individuals with Autism Spectrum Disorder (ASD) master slowly and with difficulty. We analyzed the process of learning to read of two ASD children accompanied by peers without special educational needs, aiming to pinpoint distinct aspects of their progress. We used the Observer Software Program to collect and analyze observations of their performance, which were understood as data to be classified according to previously specified codes. We believed we could demonstrate that, especially in the case of ASD children, learning is dependent on contingent responses and adequate levels of mediation. The technical team at the RLS has continuous clinical supervision. That is because we believe this supervision is what permits the team to undergo a process of de-centering, becoming more empathic and accessible to the autists. This makes the team’s intervention more efficient, because it becomes more aware of each autist’s individual characteristics, and therefore more available to respond to the autist’s needs.

وصف الملف: electronic resource

العلاقة: http://psychologyinrussia.com/volumes/index.php?article=5636Test; https://doaj.org/toc/2074-6857Test; https://doaj.org/toc/2307-2202Test

الوصول الحر: https://doaj.org/article/36b7432b95b747f29e9f43a13879bf8aTest

المؤلفون: Tirone C., Paladini A., De Maio F., Tersigni C., D'Ippolito S., Di Simone N., Santarelli G., Tana M., Lio A., Menzella N., Posteraro B., Sanguinetti M., Lanzone A., Scambia G., Vento G.

المساهمون: Tirone, Chiara, Paladini, Andrea, De Maio, Flavio, Tersigni, Chiara, D'Ippolito, Silvia, Di Simone, Nicoletta, Monzo, F. R., Santarelli, Giulia, Bianco, D. M., Tana, Milena, Lio, Alessandra, Menzella, Nicoletta, Posteraro, Brunella, Sanguinetti, Maurizio, Lanzone, Antonio, Scambia, Giovanni, Vento, Giovanni

مصطلحات موضوعية: bronchoalveolar lavage fluid (BALF), bronchopulmonary dysplasia (BPD), lung, meconium, microbiota, preterm birth (PTB), vagina, Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA

الوصف: The newborn’s microbiota composition at birth seems to be influenced by maternal microbiota. Maternal vaginal microbiota can be a determining factor of spontaneous Preterm Birth (SPPTB), the leading cause of perinatal mortality. The aim of the study is to investigate the likelihood of a causal relationship between the maternal vaginal microbiota composition and neonatal lung and intestinal microbiota profile at birth, in cases of SPPTB. The association between the lung and/or meconium microbiota with the subsequent development of bronchopulmonary dysplasia (BPD) was also investigated. Maternal vaginal swabs, newborns’ bronchoalveolar lavage fluid (BALF) (1st, 3rd, 7th day of life) and first meconium samples were collected from 20 women and 23 preterm newborns with gestational age ≤ 30 weeks (12 = SPPTB; 11 = Medically Indicated Preterm Birth–MIPTB). All the samples were analyzed for culture examination and for microbiota profiling using metagenomic analysis based on the Next Generation Sequencing (NGS) technique of the bacterial 16S rRNA gene amplicons. No significant differences in alpha e beta diversity were found between the neonatal BALF samples of SPPTB group and the MIPTB group. The vaginal microbiota of mothers with SPPTB showed a significant difference in alpha diversity with a decrease in Lactobacillus and an increase in Proteobacteria abundance. No association was found between BALF and meconium microbiota with the development of BPD. Vaginal colonization by Ureaplasma bacteria was associated with increased risk of both SPPTB and newborns’ BPD occurrence. In conclusion, an increase in α-diversity values and a consequent fall in Lactobacillus in vaginal environment could be associated to a higher risk of SPPTB. We could identify neither a specific neonatal lung or meconium microbiota profiles in preterm infants born by SPPTB nor a microbiota at birth suggestive of subsequent BPD development. Although a strict match has not been revealed between microbiota of SPPTB mother-infant couples, a relationship ...

العلاقة: info:eu-repo/semantics/altIdentifier/wos/WOS:000837012400001; volume:10; issue:N/A; firstpage:N/A; lastpage:N/A; issueyear:2022; journal:FRONTIERS IN PEDIATRICS; https://hdl.handle.net/10807/231808Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85135482714

الإتاحة: https://doi.org/10.3389/fped.2022.909962Test
https://hdl.handle.net/10807/231808Test

المؤلفون: Perri A., Fattore S., D'Andrea V., Nobile S., Tirone C., Giordano L., Tana M., Priolo F., Serrao F., Riccardi R., Prontera G., Vento G.

المساهمون: Perri, Alessandro, Fattore, Simona, D'Andrea, Vito, Sbordone, A., Patti, M. L., Nobile, Stefano, Tirone, Chiara, Giordano, Lucia, Tana, Milena, Priolo, Francesca, Serrao, Francesca, Riccardi, Riccardo, Prontera, Giorgia, Vento, Giovanni

مصطلحات موضوعية: lung ultrasonography, preterm infants, respiratory distress syndrome, Settore MED/38 - PEDIATRIA GENERALE E SPECIALISTICA

الوصف: Respiratory distress (RD) is one of the most common causes of admission to the neonatal intensive care unit. Correct diagnosis and timely intervention are crucial. Lung ultrasonography (LU) is a useful diagnostic tool for the neonatologist in the diagnosis of RD; the neonatal lung ultrasonography score (nLUS) can be used in the diagnostic process, but some authors hypothesise that it is also useful for the management of some neonatal RD. The aim of this study is to analyse the changes in nLUS score before (T0) and after (T1) the start of respiratory support with nasal CPAP in neonates over 32 weeks of age with RD. Thirty-three newborns were enrolled in this retrospective study. LU was performed before and after the start of CPAP. The median nLUS scores at T0 and T1 were 9 (IQR 7–12) and 7 (IQR 4–10), respectively, and showed a significant difference (p < 0.001). The magnitude of reduction in nLUS score, expressed as a percentage, was inversely related to the need for subsequent administration of exogenous surfactant. The study suggests the usefulness of the nLUS score in assessing the response to CPAP in neonates over 32 weeks gestational age.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/36010259; info:eu-repo/semantics/altIdentifier/wos/WOS:000845902500001; volume:12; issue:8; firstpage:N/A; lastpage:N/A; issueyear:2022; journal:DIAGNOSTICS; https://hdl.handle.net/10807/223167Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85137393206

الإتاحة: https://doi.org/10.3390/diagnostics12081909Test
https://hdl.handle.net/10807/223167Test

المؤلفون: Vento G., Ventura M. L., Pastorino R., van Kaam A. H., Carnielli V., Cools F., Dani C., Mosca F., Polglase G., Tagliabue P., Boni L., Cota F., Tana M., Tirone C., Aurilia C., Lio A., Costa S., D'Andrea V., Lucente M., Nigro G., Giordano L., Roma V., Villani P. E., Fusco F. P., Fasolato V., Colnaghi M. R., Matassa P. G., Vendettuoli V., Poggi C., Del Vecchio A., Petrillo F., Betta P., Mattia C., Garani G., Solinas A., Gitto E., Salvo V., Gargano G., Balestri E., Sandri F., Mescoli G., Martinelli S., Ilardi L., Ciarmoli E., Di Fabio S., Maranella E., Grassia C., Ausanio G., Rossi V., Motta A., Tina L. G., Maiolo K., Nobile S., Messner H., Staffler A., Ferrero F., Stasi I., Pieragostini L., Mondello I., Haass C., Consigli C., Vedovato S., Grison A., Maffei G., Presta G., Perniola R., Vitaliti M., Re M. P., De Curtis M., Cardilli V., Lago P., Tormena F., Orfeo L., Gizzi C., Massenzi L., Gazzolo D., Strozzi M. C. M., Bottino R., Pontiggia F., Berardi A., Guidotti I., Cacace C., Meli V., Quartulli L., Scorrano A., Casati A., Grappone L., Pillow J. J.

المساهمون: Vento, G., Ventura, M. L., Pastorino, R., van Kaam, A. H., Carnielli, V., Cools, F., Dani, C., Mosca, F., Polglase, G., Tagliabue, P., Boni, L., Cota, F., Tana, M., Tirone, C., Aurilia, C., Lio, A., Costa, S., D'Andrea, V., Lucente, M., Nigro, G., Giordano, L., Roma, V., Villani, P. E., Fusco, F. P., Fasolato, V., Colnaghi, M. R., Matassa, P. G., Vendettuoli, V., Poggi, C., Del Vecchio, A., Petrillo, F., Betta, P., Mattia, C., Garani, G., Solinas, A., Gitto, E., Salvo, V., Gargano, G., Balestri, E., Sandri, F., Mescoli, G., Martinelli, S., Ilardi, L., Ciarmoli, E., Di Fabio, S., Maranella, E., Grassia, C., Ausanio, G., Rossi, V., Motta, A., Tina, L. G., Maiolo, K., Nobile, S., Messner, H., Staffler, A., Ferrero, F., Stasi, I., Pieragostini, L., Mondello, I., Haass, C., Consigli, C., Vedovato, S., Grison, A., Maffei, G., Presta, G., Perniola, R., Vitaliti, M., Re, M. P., De Curtis, M., Cardilli, V., Lago, P., Tormena, F., Orfeo, L., Gizzi, C., Massenzi, L., Gazzolo, D., Strozzi, M. C. M., Bottino, R., Pontiggia, F., Berardi, A., Guidotti, I., Cacace, C., Meli, V., Quartulli, L., Scorrano, A., Casati, A., Grappone, L., Pillow, J. J.

الوصف: Background: The importance of lung recruitment before surfactant administration has been shown in animal studies. Well designed trials in preterm infants are absent. We aimed to examine whether the application of a recruitment manoeuvre just before surfactant administration, followed by rapid extubation (intubate-recruit-surfactant-extubate [IN-REC-SUR-E]), decreased the need for mechanical ventilation during the first 72 h of life compared with no recruitment manoeuvre (ie, intubate-surfactant-extubate [IN-SUR-E]). Methods: We did a randomised, unblinded, controlled trial in 35 tertiary neonatal intensive care units in Italy. Spontaneously breathing extremely preterm neonates (24 + 0 to 27 + 6 weeks' gestation) reaching failure criteria for continuous positive airway pressure within the first 24 h of life were randomly assigned (1:1) with a minimisation algorithm to IN-REC-SUR-E or IN-SUR-E using an interactive web-based electronic system, stratified by clinical site and gestational age. The primary outcome was the need for mechanical ventilation in the first 72 h of life. Analyses were done in intention-to-treat and per-protocol populations, with a log-binomial regression model correcting for stratification factors to estimate adjusted relative risk (RR). This study is registered with ClinicalTrials.gov, NCT02482766. Findings: Of 556 infants assessed for eligibility, 218 infants were recruited from Nov 12, 2015, to Sept 23, 2018, and included in the intention-to-treat analysis. The requirement for mechanical ventilation during the first 72 h of life was reduced in the IN-REC-SUR-E group (43 [40%] of 107) compared with the IN-SUR-E group (60 [54%] of 111; adjusted RR 0·75, 95% CI 0·57–0·98; p=0·037), with a number needed to treat of 7·2 (95% CI 3·7–135·0). The addition of the recruitment manoeuvre did not adversely affect the safety outcomes of in-hospital mortality (19 [19%] of 101 in the IN-REC-SUR-E group vs 37 [33%] of 111 in the IN-SUR-E group), pneumothorax (four [4%] of 101 vs seven [6%] of 111), or ...

العلاقة: info:eu-repo/semantics/altIdentifier/wos/WOS:000631356600027; volume:9; issue:2; firstpage:159; lastpage:166; numberofpages:8; journal:THE LANCET RESPIRATORY MEDICINE; http://hdl.handle.net/11380/1208215Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85089297897

الإتاحة: https://doi.org/10.1016/S2213-2600Test(20)30179-X
http://hdl.handle.net/11380/1208215Test