المؤلفون: Lisa Landino, Lydia Boike, Taylor Lain

المصدر: BioChem, Vol 4, Iss 1, Pp 18-37 (2024)

مصطلحات موضوعية: singlet oxygen, hypochlorous acid, cysteine oxidation, lactate dehydrogenase, disulfide, tryptophan fluorescence, Therapeutics. Pharmacology, RM1-950, Biochemistry, QD415-436

الوصف: Muscle lactate dehydrogenase (LDH-A) catalyzes the reduction of pyruvate to lactate, the end product of anaerobic glycolysis. LDH-A is overexpressed in many cancers prior to and even when tumors receive adequate oxygen, and lactate has multiple cellular roles. We assessed the effect of singlet oxygen and hypochlorous acid (HOCl) on mammalian LDH-A. Oxidants induced distinct patterns of protein crosslinks observed by SDS-PAGE under reducing conditions. LDH-A cysteines were detected using fluorescein-modified maleimide to assess their oxidation and accessibility. Singlet oxygen initially increased cysteine exposure, but higher doses resulted in their oxidation in addition to non-reducible covalent crosslinks. LDH-A cysteines were oxidized by micromolar HOCl (1–10 equivalents over enzyme) but were resistant to millimolar H2O2, chloramines and Angeli’s salt. HOCl oxidation inhibited LDH-A activity and yielded inter-chain disulfides observed by nonreducing SDS-PAGE. Disulfide reduction did not restore LDH-A activity that was lost due to HOCl oxidation. An irreversible conformational change induced by HOCl was detected by native gel electrophoresis and tryptophan fluorescence. In the absence of pyruvate, LDH-A enhanced NADH oxidation resulting in H2O2 formation. Singlet oxygen, but not HOCl, initiated this superoxide-dependent chain reaction. Once damaged by both singlet oxygen or HOCl, LDH-A had decreased NADH oxidation activity.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2673-6411/4/1/2Test; https://doaj.org/toc/2673-6411Test

الوصول الحر: https://doaj.org/article/acbd6d3121f246a7952a488f87456ccfTest

المؤلفون: Taylor, lain N., Kenny, Gavin N.C.

المصدر: Current Opinion in Anaesthesiology ; volume 6, issue 4, page 696-699 ; ISSN 0952-7907

الإتاحة: https://doi.org/10.1097/00001503-199308000-00019Test
https://journals.lww.com/00001503-199308000-00019Test

المؤلفون: Taylor, lain N., Kenny, Gavin N.C.

المصدر: Current Opinion in Anaesthesiology; August 1993, Vol. 6 Issue: 4 p696-699, 4p

مستخلص: The group of compounds known as non-steroidal anti-inflammatory drugs (NSAIDs) has been growing since the early 1950's. Originally there were few in number and they were used extensively for the treatment of arthropathies. More recently developed compounds have been shown to be useful in the control of postoperative pain while lacking the cardio-respiratory and emetic adverse effects associated with the use of opioid analgesics.

المؤلفون: Placher, William C., Flett, John, Purcell, Michael, Taylor, Lain, Lincoln, Andrew T., Collins, Paul M., Hastings, W. Ross, Strunk, Nathan R.

المصدر: International Journal of Systematic Theology; Jul2005, Vol. 7 Issue 3, p316-342, 27p

مصطلحات موضوعية: NONFICTION

Reviews & Products: THEOLOGICAL Ethics & Global Dynamics: In the Time of Many Worlds (Book), WORD Made Flesh: A History of Christian Thought, The (Book), FAITH Seeking Understanding: An Introduction to Christian Theology (Book), KARL Rahner: Theology & Philosophy (Book)

People: SCHWEIKER, William, MILES, Margaret R., MIGLIORE, Daniel L., KILBY, Karen

مستخلص: Books reviewed: William Schweiker, Theological Ethics and Global Dynamics: In the Time of Many Worlds. Reviewed by Brian Brock Margaret R. Miles, The Word Made Flesh: A History of Christian Thought. Reviewed by William C. Placher Daniel Migliore, Faith Seeking Understanding: An Introduction to Christian Theology. Reviewed by John Flett Karen Kilby, Karl Rahner: Theology and Philosophy. Reviewed by Michael Purcell Kevin D. Kennedy, Union with Christ and the Extent of the Atonement in Calvin. Reviewed by Iain Taylor C. Helmer and C. Landmesser, eds., One Scripture or Many? Canon from Biblical, Theological and Philosophical Perspectives. Reviewed by Andrew T. Lincoln Gabriel Flynn, Yves Congar's Vision of the Church in a World of Unbelief. Reviewed by Paul M. Collins Paul Helm and Oliver D. Crisp, eds., Jonathan Edwards: Philosophical Theologian. Reviewed by W. Ross Hastings Henrique Pinto, Foucault, Christianity and Interfaith Dialogue. Reviewed by John Flett David Lauber, Barth on the Descent into Hell: God, Atonement and the Christian Life. Reviewed by Nathan R. Strunk [ABSTRACT FROM AUTHOR]

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