المؤلفون: Godwin Okwudiri Ihegboro, Chimaobi James Ononamadu, Mujiburrahman Fadilu, Peter Prince Oghenekome, Bello Jacob, Sunday Edwin

المصدر: International Journal of Plant Based Pharmaceuticals, Vol 4, Iss 1 (2024)

مصطلحات موضوعية: Oxidative stress, Tapinanthus bangwensis, Diabetic rats, Haematopoietic markers, Silymarin tablets, Plant culture, SB1-1110

الوصف: Plant-based products are gradually replacing pharmaceuticals in treating ailments, including diabetes, due to their safety, cost-effectiveness, potency, and availability. Therefore, the current study looked into the therapeutic effect of the solvent fraction of hexane leaf extract of Tapinanthus bangwensis (HEXETACF) (Loranthaceae) in alloxan-induced pathology in diabetic rats. The biochemical parameters were estimated using analytical grade kits via spectrophotometric method. The laboratory rats were distributed into group W (five rats on feed and water), group X (seven rats + 150 mg/kg alloxan solution only), group Y (seven rats + 150 mg/kg alloxan solution + 200 mg/kg BW silymarin for 21 days), and group Z (seven rats + 150 mg/kg alloxan solution + 250 mg/kg BW HEXETACF for 21 days). The results showed that HEXETACF and silymarin (SILY) reduced blood glucose concentration by 33.77% and 34.80%, respectively, after the 21st day of treatment (p < 0.05). Additionally, alkaline phosphatase (ALP) and alanine aminotransferase (ALT) activity in SILY and HEXETACF were significantly decreased compared to the diabetic group (p < 0.05), but no significant decrease in aspartate aminotransferase (AST) activity was observed between the test samples and the diabetic group. Furthermore, the test samples lowered malondialdehyde (MDA) levels, by improving glutathione, superoxide dismutase (SOD), and catalase (CAT) activity. The HEXETACF and SILY significantly decreased triglyceride levels (TG) compared to the diabetic group at p < 0.05. They also reduced low-density lipoprotein (LDL) and cholesterol levels and increased the high-density lipoprotein levels compared to the diabetic group. Additionally, no significant decrease in serum electrolytes (Na+, K+, and Cl-), urea, and creatinine (including albumin and total protein) values was observed in HEXETACF and SILY, while hematological indices increased compared to the diabetic group. Histology results revealed that the test samples had normalized glomeruli, β-islet cells, and hepatocytes. However, a trace of mild congestion was noticed in the STDG. But edemic blood congestion was observed in the diabetic group. In conclusion, the current result demonstrated that HEXETACF may be a promising antidiabetic agent that could replace mSILY.

وصف الملف: electronic resource

العلاقة: https://ijpbp.com/index.php/ijpbp/article/view/196Test; https://doaj.org/toc/2791-7509Test

الوصول الحر: https://doaj.org/article/6f15afaf40fe4c99b6a8fb077ebe4ae8Test

المؤلفون: C. Wahua, P. Odinma

المصدر: Journal of Applied Sciences and Environmental Management, Vol 28, Iss 5 (2024)

مصطلحات موضوعية: Tapinanthus bangwensis, Loranthaceae, Morphology, Anatomy, phytochemistry, Science

الوصف: Tapinanthus bangwensis commonly known as mistletoe, is a hemi parasitic shrub of Loranthaceae, and the native range is West Tropical Africa to Chad; used in tradomedicine to remedy various ailments in Nigeria. Hence, the objective of this paper was to investigate the chemotaxonomic characteristics of Tapinanthus bangwensis (Engl. & K. Krause) Danser of Loranthaceae collected from University of Port Harcourt, Nigeria using appropriate standard methods. Results of anatomy revealed 4 to 5 rows of collenchyma at hypodermis, parenchyma domiciled in the ground tissues, vascular sclerenchyma present especially in vascular system. In the phytochemistry, alkaloids, tannins, saponins, phenolics and flavonoids were present while terpenoids absent. These information would assist for further delimitation of the species.

وصف الملف: electronic resource

العلاقة: https://www.ajol.info/index.php/jasem/article/view/270853Test; https://doaj.org/toc/2659-1502Test; https://doaj.org/toc/2659-1499Test

الوصول الحر: https://doaj.org/article/8a0cd58d09724633a0eee9b55042b5f5Test

المؤلفون: Doris Kumadoh, Michael O. Kyene, Mary-Ann Archer, Genevieve N. Yeboah, Emmanuel Adase, Maxwell Mamfe Sakyiamah, Susana Oteng-Mintah, Ofosua Adi-Dako, Christina Osei-Asare, Esther Eshun Oppong

المصدر: Scientific African, Vol 23, Iss , Pp e02025- (2024)

مصطلحات موضوعية: Herbal teas, Tapinanthus bangwensis, Phytochemical constituents, Fingerprinting, Diabetes and microbial contamination, Science

الوصف: The incorporation of traditional medicine into the mainstream health care delivery system particularly in some developing countries such as Ghana has resulted in reliable access to herbal medicines and their safe use. This has also led to a surge in the search for herbal alternatives, reformulation, and development of herbal medications in treating and managing diseases such as diabetes. Currently, the surging price of orthodox antidiabetics and their reported adverse effects from long term use has contributed to many Ghanaians switching to the use of phytomedicines as alternatives. The aim of this study is to formulate tea from the leaves of Tapinanthus bangwensis, family Loranthaceae to serve as an additional remedy for the management of diabetes.For standardization, quality control, and authentication of the formulation, FTIR, and HPLC analysis were conducted. The safety profile of the tea via determination of median lethal dose (LD50), microbial load, essential elements and toxic metal contents were also assessed. In addition, the pH, uniformity of mass, total water extractive and optimization of the extraction method were also investigated.The FTIR showed the presence of alcohols, esters, phenols and aromatic compounds. The HPLC fingerprint also showed 23 peaks with the highest peak having a retention time of 15.4 min. The extract also recorded a pH of 7.43 ± 0.02 at 26.1 °C and total water extractive of 39.37 ± 0.05 %w/w. The tea passed the uniformity of mass test with an average net mass of 3.0 ± 0.02 g. Optimum extraction of the tea was realised with 250 mL of freshly boiled water. The formulated tea was found to be safe since the determined LD50 was 50 times more than the daily dose. The microbial load, elemental and toxic metal contents were also within acceptable limits.This study has shown the possibility of introducing this tea at a safe dose as an additional herbal remedy for the management of diabetes.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2468227623004799Test; https://doaj.org/toc/2468-2276Test

الوصول الحر: https://doaj.org/article/ea1c13d645d54f09aa201fe63cc77954Test

المؤلفون: Maxwell Mamfe Sakyiamah, Paa Kwesi Gordon, Peter Bolah, Prince Kyei Baffour, Ebenezer Ehun, Olga Quasie, Doris Kumadoh, Mary-Ann Archer, Susana Oteng Mintah, Alfred Ampomah Appiah

المصدر: BMC Complementary Medicine and Therapies, Vol 23, Iss 1, Pp 1-10 (2023)

مصطلحات موضوعية: Tapinanthus bangwensis, Phytochemical composition, Antimicrobial properties, Alternative therapy, Other systems of medicine, RZ201-999

الوصف: Abstract Background Medicinal plants represent a valuable source for new effective and safe antimicrobial drugs making them an alternative therapy. Existing antimicrobial agents are costly and mostly associated with possible side effects. The aim of the present study therefore, was to assess the antimicrobial property and phytochemical composition of hydroethanolic extract of Tapinanthus bangwensis leaves and its fractions. Method T. bangwensis leaves (harvested from its host plant, Persea americana) was extracted by cold maceration with 70% ethanol and further fractionated with different organic solvents using the solvent partitioning method to obtain the crude extract, petroleum ether, chloroform, ethyl acetate and the resulting aqueous fractions. The phytochemical constituents of the extracts were screened and quantified. Also, the TLC of the extracts were analyzed to serve as a fingerprint. Using the agar diffusion and broth dilution methods, the antimicrobial properties of the extracts were assessed. Results The study showed that the hydroethanolic (70%) crude extract of T. bangwensis leaves and its fractions contain phenolic compounds, flavonoids, saponins, phytosterols and reducing sugars. The phytoconstituents were well extracted into the ethyl acetate fraction than the other fractions evidenced in the high levels (p

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2662-7671Test

الوصول الحر: https://doaj.org/article/04a2f20b2e2b4ba9b8072a0209abec86Test

المؤلفون: Stephen I Omeodu, Felicia N Okwakpam

المصدر: World Journal of Biology Pharmacy and Health Sciences 14(1) 190–198

مصطلحات موضوعية: Tapinanthus bangwensis, Serum electrolytes, Citrate buffer, Diabetic treated concentrations, Alloxan

الوصف: This study investigated the effect of aqueous extract of Tapinanthus bangwensis on some serum electrolytes in alloxan-induced diabetic rats. The effect of this plant extract was monitored on the serum concentration of potassium iron (k+), sodium ion (Na+), uric acid, bicarbonate (HCO3_), and urea. Sixty-six albino rats were used for the study arranged into eleven groups of 6 rats each. Two groups (12 rats) were used for the pilot study. Nine other groups (54 rats) were used for the experiment labelled groups 1-9. Group 1 constitute the normal control which received only feed and water, group 2 received 50mg/kg citrate buffer. Group 3 was administered alloxan solution and allowed free access to feed and water. Group 4-6 received 50mg/kg of citrate buffer and were also administered with the aqueous extract of Tapinanthus bangwensis at a dose of 250mg/kg and referred to as normal treated, concentration 1(NT conc-1), concentration 2 (NT con-2) and concentration 3 (NT conc-3) respectively. Groups 7-9 were administered 50mg/kg alloxan and different grades (5%, 7%, 10%) respectively of the aqueous extract. Blood liver and pancreatic tissues were collected into appropriately labelled sample bottles and analyzed. The result of the blood analysis showed electrolyte levels were only slightly altered with potassium ions having the least change. Bicarbonate rose from 22.67±1.15 to 24.50±1.52, potassium ions decreased from 4.45±0.14 to 4.43±0.22 and sodium ions rose from 139.33±1.63 to 143.00±2.90. The metabolite uric acid was elevated from 2.75±0.12 to 3.35±0.33. Oral administration of 250mg/kg aqueous Tapinanthus bangwensis extract to groups 7,8 and 9 of 50mg/kg, 70mg/kg, and 100mg/kg respectively, significantly decreased many of these biochemical alterations in a dose-dependent manner. 100mg/kg extract showed the highest effect in lowering the elevated parameters followed by 70mg/kg administration. The 50mg/kg dose had the least lowering effect. Histopathological results also confirmed these chemical pathological results. ...

العلاقة: https://zenodo.org/communities/wjbphsTest; https://zenodo.org/record/8037651Test; https://doi.org/10.5281/zenodo.8037651Test; oai:zenodo.org:8037651

الإتاحة: https://doi.org/10.5281/zenodo.8037651Test
https://doi.org/10.30574/wjbphs.2023.14.1.0182Test
https://doi.org/10.5281/zenodo.8037650Test
https://zenodo.org/record/8037651Test

المؤلفون: Stephen I. Omeodu, Felicia N. Okwakpam

المصدر: International Journal of Recent Research in Interdisciplinary Sciences (IJRRIS) 10(2) 11-15

مصطلحات موضوعية: Tapinanthus bangwensis, Mistletoe, serum lipid metabolites, elevated parameters, lipid profile, Alloxan, diabetes, Wistar rats, Paper Download Link (Source)

الوصف: This study investigated the effect of aqueous extract of Tapinanthus bangwensis (T. bangwensis) on some lipid profiles in alloxan-induced diabetic Wistar rats. The effects of this plant extract were monitored on the serum concentrations of triglyceride (TG), high-density lipoprotein-cholesterol (HDL-C) and cholesterol (CHOL). Sixty-six albino rats were used for the study arranged into eleven groups of six rats each. Two groups (12 rats) were used for a pilot study. Nine other groups (54 rats) were used for the experiment labeled groups 1-9. Group 1 constitutes the normal control which received only feed and water, group 2 received 50mg/kg citrate buffer. Alloxan was dissolved in 0.1M citrate buffer solution, pH 4.5. Group 3 received 50mg/kg alloxan dissolved in citrate buffer in a 1:1 ratio intraperitoneally. Groups 4-6 were treated with 50mg/kg citrate buffer with 5%, 7%, and 10% aqueous extract of T. bangwensis respectively. Groups 7-9 received 50mg/kg alloxan dissolved in citrate buffer in a 1:1 ratio intraperitoneally with 5%, 7%, and 10% aqueous extract of T. bangwensis respectively. Blood samples were collected into appropriately labeled sampled bottles and analyzed. Results of the blood analysis showed that alloxan caused diabetes mellitus in the experimental animals. Lipid profile parameters such as triglyceride (TG) (0.820±0.01), high-density lipoprotein cholesterol (HDL-CHOL) (0.480±0.00), and cholesterol (CHOL) (2.100±0.06) of the control animals were found to be within the normal range. These parameters significantly increased in the animals induced with diabetes but not treated. Treatment with the plant extract sparingly reduced the raised serum triglycerides in a dose-dependent manner. This study was able to establish the diabetogenicity of alloxan as seen in lipids, concentrations are elevated and treatment with Tapinanthus bangwensis reduced the raised serum triglycerides. Keywords: Tapinanthus bangwensis, Mistletoe, serum lipid metabolites, elevated parameters, lipid profile, Alloxan, ...

العلاقة: https://www.paperpublications.org/upload/bookTest/Tapinanthus%20bangwensis%20(Mistletoe)-20042023-1.pdf; https://zenodo.org/record/7849089Test; https://doi.org/10.5281/zenodo.7849089Test; oai:zenodo.org:7849089

الإتاحة: https://doi.org/10.5281/zenodo.7849089Test
https://doi.org/10.5281/zenodo.7849088Test
https://zenodo.org/record/7849089Test

المؤلفون: Godwin O. Ihegboro, Adamu J. Alhassan, Chimaobi J. Ononamadu, Tajudeen A. Owolarafe, Mohammed S. Sule

المصدر: Toxicology Reports, Vol 7, Iss , Pp 671-679 (2020)

مصطلحات موضوعية: Cytotoxicity, Allium cepa, Tapinanthus bangwensis, Moringa oleifera, Mitotic Index, Chromosomal aberration, Toxicology. Poisons, RA1190-1270

الوصف: Medicinal plants are considered two-edged swords. They are pharmacologically beneficial and sometimes toxic when abused. The health benefit of medicinal plant is due to their abundant phytochemical endowment. The present study is aimed at evaluating the biosafety potentials of methanol extracts/fractions of Tapinanthus bangwensis (T. bangwensis) and Moringa oleifera (M. oleifera) leaves using Allium cepa (A. cepa) model. Cytotoxicity assay was carried out by A. epa model. The cytotoxicity parameters studied were number of dividing cells, percentage mitotic index (% MI), root tip growth length and chromosomal aberrations. The phytochemistry was determined by UV-spectrophotometry while 2,2-diphenyl-1-picrylhydrazine (DPPH) and nitric oxide (NO) were used to assay antioxidant activity. The A. cepa assay result showed that the inhibitory effect of M. oleifera on root tip growth length was higher compared to T. bangwensis at 60-100mg/100ml concentrations. Decreased number of dividing cells and percentage mitotic index as concentrations increased observed indicate cytotoxicity however the acetone fractions were most cytotoxic. The frequently occurring chromosomal aberrations observed were vagrant, bridged, attached and sticky chromosomes while C-mitosis and binuclear chromosomes were not observed. Therefore cytotoxic effect was significant at 100mg/100ml. The phytochemical screening of methanol extracts/fractions of T. bangwensis and M. oleifera leaves revealed the presence of alkaloids, flavonoids, phenolics, saponins and tannins however saponins were significantly abundant in both plants compared to others. Alkaloid content was found to be low but was not detected in the acetone fractions of the plants. Methanol extracts/fractions of the plants' leaves exhibited antioxidant activities but was more prominent in the ethylacetate fraction of T. bangwensis and acetone fraction of M. oleifera. In conclusion, administration at 100mg/100ml could be unsafe for the biological system.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S221475001930486XTest; https://doaj.org/toc/2214-7500Test

الوصول الحر: https://doaj.org/article/87d5360647404c1691b91804718a8e99Test

المؤلفون: O.C. Atewolara-Odule, O.O. Aiyelaagbe, O.O. Olubomehin, A.O. Ogunmoye, R.T. Feyisola, A.S. Sanusi

المصدر: Scientific African, Vol 8, Iss , Pp e00348- (2020)

مصطلحات موضوعية: Tapinanthus bangwensis, Friedelin, Loranthaceae, Eudesmic acid, Methyl-3,4,5-trimethoxybenzoate, Science

الوصف: The search for new natural antioxidants has grown tremendously over the years because reactive oxygen species (ROS) production and oxidative stress have been linked to a large number of human degenerative diseases, such as cancer, cardiovascular diseases and diabetes. Tapinanthus bangwensis, a parasitic plant commonly known as mistletoe is employed traditionally to treat cancer, diabetes and hypertension. In this study, air-dried pulverized leaves and stem of Tapinanthus bangwensis were successively extracted with n-hexane, ethyl acetate and methanol to give the corresponding crude extracts. The extracts were purified by column chromatography and high-performance liquid chromatography. Structural elucidation was done using mass spectrometry, Fourier transform infra-red, 1D and 2D NMR spectroscopy. Friedelin, eudesmic acid and methyl-3,4,5-trimethoxybenzoate were isolated and characterized. Friedelin was isolated from the ethyl acetate extract of the stem while the two other compounds were isolated from the methanol extract of the leaves. 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used to evaluate their antioxidant activity and ascorbic acid as standard. The percentage free radical scavenging activities of the compounds are as follows: Friedelin, 73.69, methyl-3,4,5-trimethoxybenzoate, 79.33 and eudesmic acid, 87.68 which were quite comparable to 93.96 given by ascorbic acid. To the best of our knowledge, the occurrence of friedelin and eudesmic acid in Tapinanthus bangwensis is being reported for the first time. The high antioxidant activity of these compounds supports the use of this plant in the management of diabetes and hypertension among other ailments.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2468227620300867Test; https://doaj.org/toc/2468-2276Test

الوصول الحر: https://doaj.org/article/6ec7dead24474748a862e17560fb8537Test

المؤلفون: null Stephen I Omeodu, null Felicia N Okwakpam

مصطلحات موضوعية: Pulmonary and Respiratory Medicine, Tapinanthus bangwensis, Serum electrolytes, Citrate buffer, Diabetic treated concentrations, Alloxan, Pediatrics, Perinatology and Child Health

الوصف: This study investigated the effect of aqueous extract ofTapinanthus bangwensison some serum electrolytes in alloxan-induced diabetic rats. The effect of this plant extract was monitored on the serum concentration of potassium iron (k+), sodium ion (Na+), uric acid, bicarbonate (HCO3_), and urea. Sixty-six albino rats were used for the study arranged into eleven groups of 6 rats each. Two groups (12 rats) were used for the pilot study. Nine other groups (54 rats) were used for the experiment labelled groups 1-9. Group 1 constitute the normal control which received only feed and water, group 2 received 50mg/kg citrate buffer. Group 3 was administered alloxan solution and allowed free access to feed and water. Group 4-6 received 50mg/kg of citrate buffer and were also administered with the aqueous extract ofTapinanthus bangwensisat a dose of 250mg/kg and referred to as normal treated, concentration 1(NT conc-1), concentration 2 (NT con-2) and concentration 3 (NT conc-3) respectively. Groups 7-9 were administered 50mg/kg alloxan and different grades (5%, 7%, 10%) respectively of the aqueous extract. Blood liver and pancreatic tissues were collected into appropriately labelled sample bottles and analyzed. The result of the blood analysis showed electrolyte levels were only slightly altered with potassium ions having the least change. Bicarbonate rose from 22.67±1.15 to 24.50±1.52, potassium ions decreased from 4.45±0.14 to 4.43±0.22 and sodium ions rose from 139.33±1.63 to 143.00±2.90. The metabolite uric acid was elevated from 2.75±0.12 to 3.35±0.33. Oral administration of 250mg/kg aqueousTapinanthus bangwensisextract to groups 7,8 and 9 of 50mg/kg, 70mg/kg, and 100mg/kg respectively, significantly decreased many of these biochemical alterations in a dose-dependent manner. 100mg/kg extract showed the highest effect in lowering the elevated parameters followed by 70mg/kg administration. The 50mg/kg dose had the least lowering effect. Histopathological results also confirmed these chemical pathological results. The extract ofTapinanthus bangwensisis insulinogenic and thus can be a good antibiotic agent as it can improve most of the altered biochemical and physiological parameters observed during diabetes mellitus.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a620a73bc85780ef72502ccdcf9a3c3Test

المؤلفون: Edagbo, David Enuwa, Oyetunji, Olusola Jacob

المصدر: Greener Journal of Biochemisty and Biotechnology 6(1) 1-11

مصطلحات موضوعية: Tapinanthus bangwensis, Citrus sinensis, Irvingia gabonensis, infested hosts

الوصف: The presence of secondary metabolites, amino acids and sugar alcohol including protein profile in the host-parasite relations of Tapinanthus bangwensis (Tb) on the host plants, Citrus sinensis (Cs) and Irvingia gabonensis (Ig) were evaluated. Secondary metabolites contained in the host-parasite plants was observed for the Citrus-parasite (tannin - 0.35 Tb, 0.21 Cs; phenol - 0.21 Tb, 0.14 Cs; alkaloids - 1.42 Tb, 0.17 Cs g/100g) and Irvingia-parasite (tannin - 0.23 Tb, 0.18 Ig; phenol - 0.19 Tb, 0.15 Ig; alkaloids - 1.34Tb, 0.57 Ig g/100g) with comparative higher quantities in the parasite. Free amino acids and sugar alcohol contents in the leaves of host plants revealed the constituents of some of the groups in the infested (tyrosine - 12.26 Cs,14.70 Ig; aspartic acid - 12.21 Cs, 11.23 Ig ng/g) and uninfested (tyrosine - 10.76Cs,12.93 Ig; aspartic acid - 9.09 Cs, 9.79 Ig ng/g) which indicated significant higher values for the infested. Protein profiling of the Citrus leaves revealed lack of protein at 25.0 kDa band in the infested host. Assessment of metabolites and protein features in the mistletoe-host relationship affirmed presence of similar metabolites in the host-parasite entity while infested hosts had increased free amino acids and there were noticeable variations in protein banding pattern of host plants with mistletoe incidence.

العلاقة: https://zenodo.org/communities/gspublicationsTest; https://zenodo.org/record/3423638Test; https://doi.org/10.5281/zenodo.3423638Test; oai:zenodo.org:3423638

الإتاحة: https://doi.org/10.5281/zenodo.3423638Test
https://doi.org/10.5281/zenodo.3423637Test
https://zenodo.org/record/3423638Test