المؤلفون: Harini Natarajan, Andrew R. Crowley, Savannah E. Butler, Shiwei Xu, Joshua A. Weiner, Evan M. Bloch, Kirsten Littlefield, Wendy Wieland-Alter, Ruth I. Connor, Peter F. Wright, Sarah E. Benner, Tania S. Bonny, Oliver Laeyendecker, David Sullivan, Shmuel Shoham, Thomas C. Quinn, H. Benjamin Larman, Arturo Casadevall, Andrew Pekosz, Andrew D. Redd, Aaron A. R. Tobian, Margaret E. Ackerman

المصدر: mBio, Vol 12, Iss 2 (2021)

مصطلحات موضوعية: Microbiology, QR1-502

الوصف: Convalescent plasma has been deployed globally as a treatment for COVID-19, but efficacy has been mixed. Better understanding of the antibody characteristics that may contribute to its antiviral effects is important for this intervention as well as offer insights into correlates of vaccine-mediated protection.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2150-7511Test

الوصول الحر: https://doaj.org/article/06bc9967a2e644a7be0d758babdbd5adTest

المؤلفون: Maohua Pan, Tania S. Bonny, Julia Loeb, Xiao Jiang, John A. Lednicky, Arantzazu Eiguren-Fernandez, Susanne Hering, Z. Hugh Fan, Chang-Yu Wu

المصدر: mSphere, Vol 2, Iss 5 (2017)

مصطلحات موضوعية: infectious agent, infirmary, sampling, transmission, virus aerosol, Microbiology, QR1-502

الوصف: ABSTRACT The dynamics and significance of aerosol transmission of respiratory viruses are still controversial, for the major reasons that virus aerosols are inefficiently collected by commonly used air samplers and that the collected viruses are inactivated by the collection method. Without knowledge of virus viability, infection risk analyses lack accuracy. This pilot study was performed to (i) determine whether infectious (viable) respiratory viruses in aerosols could be collected from air in a real world environment by the viable virus aerosol sampler (VIVAS), (ii) compare and contrast the efficacy of the standard bioaerosol sampler, the BioSampler, with that of the VIVAS for the collection of airborne viruses in a real world environment, and (iii) gain insights for the use of the VIVAS for respiratory virus sampling. The VIVAS operates via a water vapor condensation process to enlarge aerosolized virus particles to facilitate their capture. A variety of viable human respiratory viruses, including influenza A H1N1 and H3N2 viruses and influenza B viruses, were collected by the VIVAS located at least 2 m from seated patients, during a late-onset 2016 influenza virus outbreak. Whereas the BioSampler when operated following our optimized parameters also collected virus aerosols, it was nevertheless overall less successful based on a lower frequency of virus isolation in most cases. This side-by-side comparison highlights some limitations of past studies based on impingement-based sampling, which may have generated false-negative results due to either poor collection efficiency and/or virus inactivation due to the collection process. IMPORTANCE The significance of virus aerosols in the natural transmission of respiratory diseases has been a contentious issue, primarily because it is difficult to collect or sample virus aerosols using currently available air sampling devices. We tested a new air sampler based on water vapor condensation for efficient sampling of viable airborne respiratory viruses in a student health care center as a model of a real world environment. The new sampler outperformed the industry standard device (the SKC BioSampler) in the collection of natural virus aerosols and in maintaining virus viability. These results using the VIVAS indicate that respiratory virus aerosols are more prevalent and potentially pose a greater inhalation biohazard than previously thought. The VIVAS thus appears to be a useful apparatus for microbiology air quality tests related to the detection of viable airborne viruses.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2379-5042Test

الوصول الحر: https://doaj.org/article/192c275a3eb148f99b5219012d7d2aaeTest

المؤلفون: Tania S. Bonny, John P. Driver, Taylor Paisie, Marco Salemi, John Glenn Morris, Lisa A. Shender, Lisa Smith, Carolyn Enloe, Kevin Oxenrider, Jeffery A. Gore, Julia C. Loeb, Chang-Yu Wu, John A. Lednicky

المصدر: Diseases, Vol 5, Iss 1, p 7 (2017)

مصطلحات موضوعية: Brazilian free-tailed bats, alphacoronavirus, RNA-dependent RNA polymerase (RdRp) gene, Medicine

الوصف: Bats are natural reservoirs of coronaviruses and other viruses with zoonotic potential. Florida has indigenous non-migratory populations of Brazilian free-tailed bats (Tadarida brasiliensis) that mostly roost in colonies in artificial structures. Unlike their counterparts in Brazil and Mexico, the viruses harbored by the Florida bats have been underexplored. We report the detection of an alphacoronavirus RNA-dependent RNA polymerase (RdRp) gene sequence in the feces of two of 19 different T. brasiliensis that were capture/release bats that had been evaluated for overall health. The RdRp sequence is similar but not identical to previously detected sequences in the feces of two different species of bats (T. brasiliensis and Molossus molossus) in Brazil. In common with the experience of others doing similar work, attempts to isolate the virus in cell cultures were unsuccessful. We surmise that this and highly related alphacoronavirus are carried by Brazilian free-tailed bats living in a wide eco-spatial region. As various coronaviruses (CoVs) that affect humans emerged from bats, our study raises the question whether CoVs such as the one detected in our work are yet-to-be-detected pathogens of humans and animals other than bats.

وصف الملف: electronic resource

العلاقة: http://www.mdpi.com/2079-9721/5/1/7Test; https://doaj.org/toc/2079-9721Test

الوصول الحر: https://doaj.org/article/d073be62ab2a4236a82268657680b03cTest

المؤلفون: Atiqur Rahman, Tania S. Bonny, Siriporn Stonsaovapak, Chiraporn Ananchaipattana

المصدر: Journal of Pathogens, Vol 2011 (2011)

مصطلحات موضوعية: Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502

الوصف: Yersinia enterocolitica is the most common bacteriological cause of gastrointestinal disease in many developed and developing countries. Although contaminated food is the main source of human infection due to Y. enterocolitica, animal reservoir and contaminated environment are also considered as other possible infection sources for human in epidemiological studies. Molecular based epidemiological studies are found to be more efficient in investigating the occurrence of human pathogenic Y. enterocolitica in natural samples, in addition to conventional culture based studies.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2090-3065Test

الوصول الحر: https://doaj.org/article/8f95f96129bd401f99887b49ed49a52bTest

المؤلفون: Sharada Saraf, Xianming Zhu, Ruchee Shrestha, Tania S Bonny, Owen R Baker, Evan J Beck, Reinaldo E Fernandez, Yolanda Eby, Olivia Akinde, Jessica E Ruff, Patrizio Caturegli, Andrew D Redd, Evan M Bloch, Thomas C Quinn, Aaron A R Tobian, Oliver Laeyendecker

المصدر: PLoS ONE, Vol 17, Iss 6, p e0264298 (2022)

مصطلحات موضوعية: Medicine, Science

الوصف: The association between COVID-19 symptoms and antibody responses against SARS-CoV-2 is poorly characterized. We analyzed antibody levels in individuals with known SARS-CoV-2 infection to identify potential antibody-symptom associations. Convalescent plasma from 216 SARS-CoV-2 RNA+ individuals with symptomatology information were tested for the presence of IgG to the spike S1 subunit (Euroimmun ELISA), IgG to receptor binding domain (RBD, CoronaCHEK rapid test), and for IgG, IgA, and IgM to nucleocapsid (N, Bio-Rad ELISA). Logistic regression was used to estimate the odds of having a COVID-19 symptom from the antibody response, adjusting for sex and age. Cough strongly associated with antibodies against S1 (adjusted odds ratio [aOR] = 5.33; 95% CI from 1.51 to 18.86) and RBD (aOR = 4.36; CI 1.49, 12.78). In contrast, sore throat significantly associated with the absence of antibodies to S1 and N (aOR = 0.25; CI 0.08, 0.80 and aOR = 0.31; 0.11, 0.91). Similarly, lack of symptoms associated with the absence of antibodies to N and RBD (aOR = 0.16; CI 0.03, 0.97 and aOR = 0.16; CI 0.03, 1.01). Cough appeared to be correlated with a seropositive result, suggesting that SARS-CoV-2 infected individuals exhibiting lower respiratory symptoms generate a robust antibody response. Conversely, those without symptoms or limited to a sore throat while infected with SARS-CoV-2 were likely to lack a detectable antibody response. These findings strongly support the notion that severity of infection correlates with robust antibody response.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/22271429d642482db6fa68fa3edbb8ccTest

المؤلفون: Massimiliano S. Tagliamonte, Sarah K. White, Md. Mahbubul Alam, David A. Ostrov, Julia C. Loeb, Sonese Chavannes, J. Glenn Morris, Marco Salemi, Maha A. Elbadry, Caroline J. Stephenson, Taina Telisma, Carla Mavian, Tania S. Bonny, Valery Madsen Beau De Rochars, John A. Lednicky

المصدر: Nature

مصطلحات موضوعية: Genetics, Multidisciplinary, Family Coronaviridae, SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Evolutionary change, Biology, medicine.disease_cause, Article, Computational biology and bioinformatics, Porcine deltacoronavirus, Pandemic, medicine, Adaptation, Gene, Coronavirus

الوصف: Coronaviruses have caused three major epidemics since 2003, including the ongoing SARS-CoV-2 pandemic. In each case, the emergence of coronavirus in our species has been associated with zoonotic transmissions from animal reservoirs1,2, underscoring how prone such pathogens are to spill over and adapt to new species. Among the four recognized genera of the family Coronaviridae, human infections reported so far have been limited to alphacoronaviruses and betacoronaviruses3–5. Here we identify porcine deltacoronavirus strains in plasma samples of three Haitian children with acute undifferentiated febrile illness. Genomic and evolutionary analyses reveal that human infections were the result of at least two independent zoonoses of distinct viral lineages that acquired the same mutational signature in the genes encoding Nsp15 and the spike glycoprotein. In particular, structural analysis predicts that one of the changes in the spike S1 subunit, which contains the receptor-binding domain, may affect the flexibility of the protein and its binding to the host cell receptor. Our findings highlight the potential for evolutionary change and adaptation leading to human infections by coronaviruses outside of the previously recognized human-associated coronavirus groups, particularly in settings where there may be close human–animal contact.
The presence of porcine deltacoronavirus has been detected in three children from Haiti that could have originated from zoonotic spillover.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2c9f76b969b6734f9ac2ee5bf6150f9Test
https://doi.org/10.1038/s41586-021-04111-zTest

المؤلفون: Morgan Keruly, Imani Burgess, Jernelle Miller, Aaron A.R. Tobian, Patrizio Caturegli, Andrew Pekosz, Eshan U. Patel, William Clarke, Andrew D. Redd, David J. Sullivan, Yolanda Eby, Owen R Baker, Evan M. Bloch, Haley A Schmidt, Ruchee Shrestha, Tania S. Bonny, Thomas C. Quinn, Ethan Klock, Kirsten Littlefield, Arturo Casadevall, Charles S Kirby, Oliver Laeyendecker, Sarah E. Benner, Reinaldo E Fernandez, Shmuel Shoham

المصدر: The Journal of Infectious Diseases

مصطلحات موضوعية: Adult, Male, 0301 basic medicine, Convalescent plasma, Adolescent, Coronavirus disease 2019 (COVID-19), Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Antibody Affinity, Blood Donors, chemical and pharmacologic phenomena, Antibodies, Viral, Cohort Studies, Young Adult, 03 medical and health sciences, symbols.namesake, 0302 clinical medicine, anti-nucleocapsid, avidity, Major Article, Humans, Immunology and Allergy, Medicine, Avidity, 030212 general & internal medicine, Poisson regression, COVID-19 Serotherapy, SARS-CoV-2, anti-spike, business.industry, Immunization, Passive, Antibody titer, COVID-19, Middle Aged, Igg avidity, Antibodies, Neutralizing, Titer, AcademicSubjects/MED00290, Cross-Sectional Studies, 030104 developmental biology, Infectious Diseases, Immunoglobulin G, convalescent plasma, Spike Glycoprotein, Coronavirus, Immunology, Linear Models, symbols, Female, business

الوصف: Background Convalescent plasma therapy is a leading treatment for conferring temporary immunity to COVID-19–susceptible individuals or for use as post-exposure prophylaxis. However, not all recovered patients develop adequate antibody titers for donation and the relationship between avidity and neutralizing titers is currently not well understood. Methods SARS-CoV-2 anti-spike and anti-nucleocapsid IgG titers and avidity were measured in a longitudinal cohort of COVID-19 hospitalized patients (n = 16 individuals) and a cross-sectional sample of convalescent plasma donors (n = 130). Epidemiologic correlates of avidity were examined in donors by linear regression. The association of avidity and a high neutralizing titer (NT) were also assessed in donors using modified Poisson regression. Results Antibody avidity increased over duration of infection and remained elevated. In convalescent plasma donors, higher levels of anti-spike avidity were associated with older age, male sex, and hospitalization. Higher NTs had a stronger positive correlation with anti-spike IgG avidity (Spearman ρ = 0.386; P Conclusions SARS-CoV-2 antibody avidity correlated with duration of infection and higher neutralizing titers, suggesting a potential alternative screening parameter for identifying optimal convalescent plasma donors.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bdd05e0e7c1f563e3f8d551609cc3faTest
https://doi.org/10.1093/infdis/jiaa581Test

المؤلفون: Rebecca Rose, Diane M. Brown, Aaron A.R. Tobian, Andrew D. Redd, Niraj M. Desai, Nicole A. Turgeon, Charles S. Kirby, Dorry L. Segev, Michael Seisa, Tania S. Bonny, Craig Martens, Christine M. Durand, Sander Florman, Christos Petropoulos, Rachel J. Friedman-Moraco

المصدر: Lancet HIV

مصطلحات موضوعية: Male, 0301 basic medicine, medicine.medical_specialty, Epidemiology, medicine.medical_treatment, Immunology, HIV superinfection, Liver transplantation, medicine.disease_cause, Article, Organ transplantation, 03 medical and health sciences, 0302 clinical medicine, Antiretroviral Therapy, Highly Active, Virology, Internal medicine, HIV Seropositivity, Humans, Mass Screening, Medicine, Prospective Studies, 030212 general & internal medicine, Phylogeny, Aged, business.industry, virus diseases, Cancer, Sequence Analysis, DNA, Middle Aged, Viral Load, medicine.disease, Kidney Transplantation, 030112 virology, Reverse transcriptase, CD4 Lymphocyte Count, Liver Transplantation, Transplantation, Infectious Diseases, pol Gene Products, Human Immunodeficiency Virus, Superinfection, Female, business, Viral load

الوصف: BACKGROUND: One of the primary risks of HIV-positive to HIV-positive organ transplantation is loss of virological control because of donor-derived HIV superinfection, which occurs when an HIV-positive individual becomes infected with a new distinct HIV strain. In this study, as part of the larger HIV Organ Policy Equity pilot study, HIV-positive to HIV-positive kidney and liver transplant recipients in the USA were examined for evidence of sustained donor-derived HIV superinfection. METHODS: In this multicentre, prospective, observational study, HIV-positive to HIV-positive kidney and liver transplant recipients were followed in three hospitals in the USA. Candidates with well controlled HIV infection on ART, no active opportunistic infections, and minimum CD4 T-cell counts (>100 cells per μL for liver and >200 cells per μL for kidney per federal guidelines) were eligible to receive a kidney or liver from deceased HIV-positive donors without active infections or neoplasm. Peripheral blood mononuclear cells were collected from donor–recipient pairs at the time of transplantation, and from recipients at several timepoints up to 3 years after transplantation. Donor samples were assessed for HIV RNA viral load, CD4 cell count, and antiretroviral drug-resistant mutations. Donor and recipient HIV proviral DNA, and viral RNA from the viraemic timepoint were sequenced using a site-directed next-generation sequencing assay for the reverse transcriptase and gp41 genes. Neighbour-joining phylogenetic trees and direct sequence comparison were used to detect the presence of HIV superinfection. This study is registered with ClinicalTrials.gov, NCT02602262. FINDINGS: 14 HIV-positive to HIV-positive kidney and eight liver transplant recipients were followed from March, 2016, to July, 2019. 17 recipients had adequate viral sequences allowing for HIV superinfection assessment. Eight donors were suppressed (viral load

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ae72a161c3a24c4405632525a258efaTest
https://doi.org/10.1016/s2352-3018Test(20)30200-9

المؤلفون: Ruth I Connor, Shmuel Shoham, Wendy Wieland-Alter, Andrew Pekosz, Tania S. Bonny, H. Benjamin Larman, Peter F. Wright, Margaret E. Ackerman, Andrew D. Redd, Oliver Laeyendecker, Sarah E. Benner, Shiwei Xu, Kirsten Littlefield, Arturo Casadevall, Joshua A. Weiner, David J. Sullivan, Andrew R. Crowley, Harini Natarajan, Savannah E. Butler, Thomas C. Quinn, Evan M. Bloch, Aaron A.R. Tobian

المصدر: mBio
mBio, Vol 12, Iss 2 (2021)

مصطلحات موضوعية: Male, Cell, Antibodies, Viral, Neutralization, Cohort Studies, 0302 clinical medicine, Antibody Specificity, antibody, Multiplex, Antigens, Viral, Complement Activation, media_common, Antibody-dependent cell-mediated cytotoxicity, 0303 health sciences, biology, Convalescence, Middle Aged, QR1-502, medicine.anatomical_structure, 030220 oncology & carcinogenesis, convalescent plasma, Female, Antibody, ADCC, IgA, Research Article, Adult, IgG, Phagocytosis, media_common.quotation_subject, Microbiology, Biophysical Phenomena, 03 medical and health sciences, Young Adult, Virology, medicine, Humans, COVID-19 Serotherapy, 030304 developmental biology, Aged, business.industry, SARS-CoV-2, Antibody-Dependent Cell Cytotoxicity, Immunization, Passive, COVID-19, neutralization, Editor's Pick, Antibodies, Neutralizing, Complement system, Immunology, biology.protein, business, functional antibody response

الوصف: Convalescent plasma has been deployed globally as a treatment for COVID-19, but efficacy has been mixed. Better understanding of the antibody characteristics that may contribute to its antiviral effects is important for this intervention as well as offer insights into correlates of vaccine-mediated protection.
Convalescent plasma is a promising therapy for coronavirus disease 2019 (COVID-19), but the antibody characteristics that contribute to efficacy remain poorly understood. This study analyzed plasma samples from 126 eligible convalescent blood donors in addition to 15 naive individuals, as well as an additional 20 convalescent individuals as a validation cohort. Multiplexed Fc Array binding assays and functional antibody response assays were utilized to evaluate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody composition and activity. Donor convalescent plasma samples contained a range of antibody cell- and complement-mediated effector functions, indicating the diverse antiviral activity of humoral responses observed among recovered individuals. In addition to viral neutralization, convalescent plasma samples contained antibodies capable of mediating such Fc-dependent functions as complement activation, phagocytosis, and antibody-dependent cellular cytotoxicity against SARS-CoV-2. Plasma samples from a fraction of eligible donors exhibited high activity across all activities evaluated. These polyfunctional plasma samples could be identified with high accuracy with even single Fc Array features, whose correlation with polyfunctional activity was confirmed in the validation cohort. Collectively, these results expand understanding of the diversity of antibody-mediated antiviral functions associated with convalescent plasma, and the polyfunctional antiviral functions suggest that it could retain activity even when its neutralizing capacity is reduced by mutations in variant SARS-CoV-2.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82ddf5f765596eaec8bc58085b255459Test
http://europepmc.org/articles/PMC8092262Test

المؤلفون: Sarah K. White, Md. Mahbubul Alam, Tania S. Bonny, Sonese Chavannes, Massimiliano S. Tagliamonte, David A. Ostrov, Julia C. Loeb, Marco Salemi, De Rochars Vmb, Caroline J. Stephenson, Taina Telisma, John A. Lednicky, John Glenn Morris, Carla Mavian, Maha A. Elbadry

المصدر: medRxiv
article-version (status) pre
article-version (number) 1

مصطلحات موضوعية: Male, Models, Molecular, Deltacoronavirus, Swine, medicine.disease_cause, Alphacoronavirus, Viral Zoonoses, Article, Convergent evolution, Pandemic, Chlorocebus aethiops, medicine, Animals, Humans, Amino Acid Sequence, Child, Vero Cells, Conserved Sequence, Phylogeny, Coronavirus, Genetics, Gammacoronavirus, biology, Transmission (medicine), Bayes Theorem, biology.organism_classification, Haiti, Mutation, Female, Coronavirus Infections, Betacoronavirus

الوصف: Coronaviruses have caused three major epidemics since 2003, including the ongoing SARS-CoV-2 pandemic. In each case, coronavirus emergence in our species has been associated with zoonotic transmissions from animal reservoirs1,2, underscoring how prone such pathogens are to spill over and adapt to new species. Among the four recognized genera of the family Coronaviridae – Alphacoronavirus, Betacoronavirus, Deltacoronavirus, Gammacoronavirus, – human infections reported to date have been limited to alpha- and betacoronaviruses3. We identify, for the first time, porcine deltacoronavirus (PDCoV) strains in plasma samples of three Haitian children with acute undifferentiated febrile illness. Genomic and evolutionary analyses reveal that human infections were the result of at least two independent zoonoses of distinct viral lineages that acquired the same mutational signature in the nsp15 and the spike glycoprotein genes by convergent evolution. In particular, structural analysis predicts that one of the changes in the Spike S1 subunit, which contains the receptor-binding domain, may affect protein’s flexibility and binding to the host cell receptor. Our findings not only underscore the ability of deltacoronaviruses to adapt and potentially lead to human-to-human transmission, but also raise questions about the role of such transmissions in development of pre-existing immunity to other coronaviruses, such as SARS-CoV-2.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23b3e6a70a869dd31f9b8552777bfc2cTest
http://europepmc.org/articles/PMC8010738Test