المؤلفون: Aya Shimomura, Yuki Nishida, Shion Yamamoto, Norio Suganuma, Satoshi Watanabe, Toyoaki Anai, Susumu Arima, Akiyoshi Tominaga, Akihiro Suzuki

المصدر: Plant Production Science, Vol 27, Iss 2, Pp 137-149 (2024)

مصطلحات موضوعية: Glycine max, symbiosis, nitrogen fixation, SEN1, near-isogenic lines (NILs), plant growth, vacuolar iron transporter-like protein (VTL), Plant culture, SB1-1110

الوصف: ABSTRACTIt is well known that the growth of legumes is greatly affected not only by fertilizer nitrogen but also by fixed nitrogen by symbiosis with rhizobia. We have reported that a polymorphism in SEN1, which is essential for nitrogen fixation in Lotus japonicus, could be used to enhance nitrogen fixation and plant growth. In this study, we examined whether the same strategy could be applied to Glycine max. Sequencing of orthologs of the LjSEN1 gene in 38 soybean cultivars revealed that the polymorphism occurred at Glyma.08g076300. We defined the standard sequence that most of the cultivars retained as Peking type SEN1, and the mutated sequence found in Enrei as Enrei type SEN1. To investigate the effect of genotype differences, we generated near-isogenic lines by crossing Enrei with Fukuyutaka, which has Peking type SEN1, and analyzed their characteristics. The results showed that Enrei type enhanced nitrogen fixation activity and promoted plant growth. Since soybean SEN1 has been reported to be a homolog of vacuolar iron transporter-like protein, we investigated the iron content in the nodules. In the result, the iron content in the nodules of Enrei type was significantly higher than that of Peking type. Iron is known to be present in the active center of nitrogenase, and the high iron content may have contributed to the increased nitrogen fixation activity. These results suggest that Enrei type SEN1 is a nitrogen fixation enhancing gene and that introduction of Enrei type SEN1 may reduce nitrogen fertilizer application and increase yield.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1343-943XTest; https://doaj.org/toc/1349-1008Test

الوصول الحر: https://doaj.org/article/018adf7dbec34853819ebcc6feaec067Test

المؤلفون: Akihiro Suzuki, Aya Shimomura, Ikuko Kusaba, Kanzi Ishimaru, Mareshige Kojoma, Shion Yamamoto, Susumu Arima, Takahiro Nakao, 下村 彩, 中尾 隆寛, 山本 士温, 有馬 進, 石丸 幹二, 草場 郁子, 鈴木 章弘, 高上馬 希重

المصدر: Abstracts of Meeting of the CSSJ. 2023, :64

المؤلفون: Akihiro Suzuki, Akiyoshi Tominaga, Aya Shimomura, Kirin Takemura, Michio Onjo, Norio Suganuma, Satoshi Watanabe, Susumu Arima, Toyoaki Anai, Yuki Nishida, 下村 彩, 富永 晃好, 有馬 進, 武村 希鈴, 渡邊 啓史, 穴井 豊昭, 菅沼 教生, 西田 雄輝, 遠城 道雄, 鈴木 章弘

المصدر: Abstracts of Meeting of the CSSJ. 2023, :167

المؤلفون: Akihiro Suzuki, Akiyoshi Tominaga, Norio Suganuma, Satoshi Watanabe, Susumu Arima, Toyoaki Anai, Yuki Nishida, 富永 晃好, 有馬 進, 渡邊 啓史, 穴井 豊昭, 菅沼 教生, 西田 雄輝, 鈴木 章弘

المصدر: Abstracts of Meeting of the CSSJ. 2022, :89

المؤلفون: Akihiro Suzuki, Emi Yamada, Hiroko Maita, Ikuko Kusaba, Kanji Ishimaru, Mareshige Kojoma, Ryo Akashi, Ryota Chijiiwa, Shusei Sato, Susumu Arima, Takahiro Nakao

المصدر: Plant Biotechnology. 2021, 38(1):57

المؤلفون: Aya Shimomura, Ayumi Naka, Nobuyuki Miyazaki, Sayaka Moriuchi, Susumu Arima, Shusei Sato, Hideki Hirakawa, Makoto Hayashi, Maskit Maymon, Ann M. Hirsch, Akihiro Suzuki

المصدر: Molecular Plant-Microbe Interactions, Vol 29, Iss 10, Pp 786-796 (2016)

مصطلحات موضوعية: Microbiology, QR1-502, Botany, QK1-989

الوصف: In many legumes, roots that are exposed to light do not form nodules. Here, we report that blue light inhibits nodulation in Lotus japonicus roots inoculated with Mesorhizobium loti. Using RNA interference, we suppressed the expression of the phototropin and cryptochrome genes in L. japonicus hairy roots. Under blue light, plants transformed with an empty vector did not develop nodules, whereas plants exhibiting suppressed expression of cry1 and cry2 genes formed nodules. We also measured rhizobial growth to investigate whether the inhibition of nodulation could be caused by a reduced population of rhizobia in response to light. Although red light had no effect on rhizobial growth, blue light had a strong inhibitory effect. Rhizobial growth under blue light was partially restored in signature-tagged mutagenesis (STM) strains in which LOV-HK/PAS- and photolyase-related genes were disrupted. Moreover, when Ljcry1A and Ljcry2B-silenced plants were inoculated with the STM strains, nodulation was additively increased. Our data show that blue light receptors in both the host plant and the symbiont have a profound effect on nodule development. The exact mechanism by which these photomorphogenetic responses function in the symbiosis needs further study, but they are clearly involved in optimizing legume nodulation.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/0894-0282Test; https://doaj.org/toc/1943-7706Test

الوصول الحر: https://doaj.org/article/77677c59029d4d6ca52bd8c7667cc451Test

المؤلفون: Akihiro Suzuki, Kanji Ishimaru, Mareshige Kojoma, Megumi Harada, Megumi Yamada, Ryouta Chijiiwa, Susumu Arima, Takahiro Nakao, 中尾 隆寛, 千々岩 諒汰, 原田 恵, 山田 恵美, 有馬 進, 石丸 幹二, 鈴木 章弘, 高上馬 希重

المصدر: Abstracts of Meeting of the CSSJ. 2019, :92

المؤلفون: Akihiro Suzuki, Rika Kiyota, Susumu Arima, Yoshitomo Hideshima, 有馬 進, 清田 梨華, 秀島 好知, 鈴木 章弘

المصدر: 日本作物学会紀事 / Japanese Journal of Crop Science. 2019, 88(2):125

المؤلفون: Akihiro Suzuki, Hidenori Kawazumi, Katsuya Hard, Natsumi Nakashima, Norio Suganuma, Ryouta Chijiiwa, Satori Kawano, Satoshi Watanabe, Susumu Arima, Takahiro Nakao, Toyoaki Anai, Yuka Egami, 中尾 隆寛, 中島 菜摘, 千々岩 諒汰, 原田 克哉, 有馬 進, 江上 由佳, 河済 英紀, 河野 里実, 渡邊 啓史, 穴井 豊昭, 菅沼 教生, 鈴木 章弘

المصدر: Abstracts of Meeting of the CSSJ. 2018, :123

المؤلفون: Xin Zhao, Shao-Hui Zheng, F. Fatichin, Akihiro Suzuki, Susumu Arima

المصدر: Plant Production Science, Vol 17, Iss 1, Pp 103-108 (2014)

مصطلحات موضوعية: Leaf tissue, Nitrogen accumulation, Nitrogen redistribution, Seed yield, Soybean, Plant culture, SB1-1110

الوصف: A large amount of nitrogen is redistributed from vegetative organs to the seeds during seed filling in soybean (Glycine max [L.] Merrill). However, the effect of nitrogen redistributed from leaves on the seed yield production is not clear. We evaluated the varietal difference in nitrogen redistribution and its contribution to the seed yield. Ten soybean cultivars were cultivated under conventional conditions in the field in Saga, Japan. The plant samples were collected at various reproductive stages, and then the nitrogen contents in each part were determined. The redistributed nitrogen was estimated by the difference in the nitrogen contents of leaves between the plants at the R5 and R7 stages. The nitrogen content of leaves began decreasing after R5 stage in all cultivars, indicating the start of nitrogen redistribution. About 13.8% to 37.9% of the total nitrogen in the seeds was estimated to have been redistributed from the leaf tissues in the ten cultivars. The seed yield was correlated positively with the amount of redistributed nitrogen from leaves but neither with the nitrogen concentration in the leaves at R5 nor with the proportion of redistributed nitrogen in the seeds. However, in high seed yielding years, 2008 and 2009, the seed yield was not associated with nitrogen redistribution; and the lowest nitrogen redistribution was associated with a relatively high seed yield in Tamahomare. Our results indicated that redistribution of a large amount of nitrogen does not always contribute to high seed yielding, implying the direct nitrogen uptake during seed filling could be more important factor for high seed yielding depending on the cultivars.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1343-943XTest; https://doaj.org/toc/1349-1008Test

الوصول الحر: https://doaj.org/article/da470ffc83e34be789b8e1e3a1eff1ffTest