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1دورية أكاديمية
المؤلفون: Xing, QR, El Farran, CA, Gautam, P, Chuah, YS, Warrier, T, Toh, CXD, Kang, NY, Sugii, S, Chang, YT, Xu, J, Collins, JJ, Daley, GQ, Li, H, Zhang, LF, Loh, YH
المساهمون: Massachusetts Institute of Technology. Institute for Medical Engineering & Science, Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Synthetic Biology Center
المصدر: Science Advances
الوصف: Cellular reprogramming suffers from low efficiency especially for the human cells. To deconstruct the heterogeneity and unravel the mechanisms for successful reprogramming, we adopted single-cell RNA sequencing (scRNA-Seq) and single-cell assay for transposase-accessible chromatin (scATAC-Seq) to profile reprogramming cells across various time points. Our analysis revealed that reprogramming cells proceed in an asynchronous trajectory and diversify into heterogeneous subpopulations. We identified fluorescent probes and surface markers to enrich for the early reprogrammed human cells. Furthermore, combinatory usage of the surface markers enabled the fine segregation of the early-intermediate cells with diverse reprogramming propensities. scATAC-Seq analysis further uncovered the genomic partitions and transcription factors responsible for the regulatory phasing of reprogramming process. Binary choice between a FOSL1 and a TEAD4-centric regulatory network determines the outcome of a successful reprogramming. Together, our study illuminates the multitude of diverse routes transversed by individual reprogramming cells and presents an integrative roadmap for identifying the mechanistic part list of the reprogramming machinery.
وصف الملف: application/pdf
العلاقة: Science Advances; https://hdl.handle.net/1721.1/133493Test
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2دورية أكاديمية
المؤلفون: Xing, Qiao Rui, El Farran, C. A., Gautam, P., Chuah, Y. S., Warrier, T., Toh, C. X. D., Kang, N. Y., Sugii, S., Chang, Y. T., Xu, J., Collins, J. J., Daley, G. Q., Li, H., Zhang, Li-Feng, Loh, Y. H.
المساهمون: School of Biological Sciences
مصطلحات موضوعية: Science::Biological sciences, Chromosomes, Machinery
الوصف: Cellular reprogramming suffers from low efficiency especially for the human cells. To deconstruct the heterogeneity and unravel the mechanisms for successful reprogramming, we adopted single-cell RNA sequencing (scRNA-Seq) and single-cell assay for transposase-accessible chromatin (scATAC-Seq) to profile reprogramming cells across various time points. Our analysis revealed that reprogramming cells proceed in an asynchronous trajectory and diversify into heterogeneous subpopulations. We identified fluorescent probes and surface markers to enrich for the early reprogrammed human cells. Furthermore, combinatory usage of the surface markers enabled the fine segregation of the early-intermediate cells with diverse reprogramming propensities. scATAC-Seq analysis further uncovered the genomic partitions and transcription factors responsible for the regulatory phasing of reprogramming process. Binary choice between a FOSL1 and a TEAD4-centric regulatory network determines the outcome of a successful reprogramming. Together, our study illuminates the multitude of diverse routes transversed by individual reprogramming cells and presents an integrative roadmap for identifying the mechanistic part list of the reprogramming machinery. ; Agency for Science, Technology and Research (A*STAR) ; Ministry of Education (MOE) ; Ministry of Health (MOH) ; National Medical Research Council (NMRC) ; National Research Foundation (NRF) ; Published version ; H.L. is supported by the NIH (AG056318, AG61796, and CA208517), the Glenn Foundation for Medical Research, Mayo Clinic Center for Biomedical Discovery, Center for Individualized Medicine, Mayo Clinic Cancer Center, and the David F. and Margaret T. Grohne Cancer Immunology and Immunotherapy Program. L.F.Z. is supported by the Singapore Ministry of Education Academic Research Fund (MOE2015-T2-1-093) and Singapore National Research Foundation under its Cooperative Basic Research Grant administered by the Singapore Ministry of Health’s National Medical Research Council ...
وصف الملف: application/pdf
العلاقة: MOE2015-T2-1-093; NMRC/CBRG/0092/2015; NRFI2018-02; Science Advances; Xing, Q. R., El Farran, C. A., Gautam, P., Chuah, Y. S., Warrier, T., Toh, C. X. D., . . . Loh, Y. H. (2020). Diversification of reprogramming trajectories revealed by parallel single-cell transcriptome and chromatin accessibility sequencing. Science Advances, 6(37), eaba1190-. doi:10.1126/sciadv.aba1190; https://hdl.handle.net/10356/145388Test; 37
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3دورية أكاديمية
المؤلفون: Widiasih, D. A., Ido, N., Omoe, K., Sugii, S., Shinagawa, K.
المصدر: Epidemiology and Infection, 2004 Jan 01. 132(1), 67-75.
الوصول الحر: https://www.jstor.org/stable/3865845Test
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4مؤتمر
المؤلفون: Takahashi, H., Nasa, Y., Takamasu, E., Sugii, S., Yokogawa, N.
المصدر: SLE, Sjögren’s and APS – treatment
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5مؤتمر
المؤلفون: Nasa, Y., Yokokawa, N., Takahashi, H., Takamasu, E., Sugii, S.
المصدر: SLE, Sjögren’s and APS – clinical aspects (other than treatment)
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6مؤتمر
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7مؤتمر
المؤلفون: Yokogawa, N., Komiya, A., Shimada, K., Nishino, J., Sugii, S., Tohma, S.
المصدر: Rheumatoid arthritis – prognosis, predictors and outcome
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8مؤتمر
المؤلفون: Kise, T., Shimada, K., Miyoshi, Y., Yokogawa, N., Sugii, S.
المصدر: Epidemiology, risk factors for disease or disease progression
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9دورية أكاديمية
المؤلفون: Sriram, S., Yuan, C., Chakraborty, S., Tay, W., Park, M., Shabbir, A., Toh, S.-A., Han, W., Sugii, S.
المساهمون: DEPT OF MEDICINE, DUKE-NUS MEDICAL SCHOOL, DEPT OF SURGERY
المصدر: Scopus OA2019
مصطلحات موضوعية: Ascorbic acid, Intra-abdominal fat, Mesenchymal stromal cells, Oxidative stress, Reactive oxygen species, Subcutaneous fat
الوصف: 10.1186/s13287-019-1240-y ; Stem Cell Research and Therapy ; 10 ; 1 ; 141
العلاقة: Sriram, S., Yuan, C., Chakraborty, S., Tay, W., Park, M., Shabbir, A., Toh, S.-A., Han, W., Sugii, S. (2019). Oxidative stress mediates depot-specific functional differences of human adipose-derived stem cells. Stem Cell Research and Therapy 10 (1) : 141. ScholarBank@NUS Repository. https://doi.org/10.1186/s13287-019-1240-yTest; https://scholarbank.nus.edu.sg/handle/10635/210774Test
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10دورية أكاديمية
المؤلفون: Yuan, C, Chakraborty, S, Chitta, K.K, Subramanian, S, Lim, T.E, Han, W, Bhanu Prakash, K.N, Sugii, S
المساهمون: MECHANOBIOLOGY INSTITUTE, DUKE-NUS MEDICAL SCHOOL
المصدر: Unpaywall 20201031
مصطلحات موضوعية: cell nucleus receptor, 3T3-L1 cell line, accuracy, adipocyte, adipogenesis, adipose derived mesenchymal stem cell, adipose tissue cell, algorithm, animal cell, Article, cell density, cell differentiation, controlled study, embryoid body, fast adipogenesis tracking system, human, human cell, induced pluripotent stem cell, ligand binding, mesenchymal stem cell, mouse, nonhuman, priority journal, quantitative analysis
الوصف: 10.1186/s13287-019-1141-0 ; Stem Cell Research and Therapy ; 10 ; 1 ; 38
العلاقة: Yuan, C, Chakraborty, S, Chitta, K.K, Subramanian, S, Lim, T.E, Han, W, Bhanu Prakash, K.N, Sugii, S (2019). Fast Adipogenesis Tracking System (FATS) - A robust, high-throughput, automation-ready adipogenesis quantification technique 10 Technology 1004 Medical Biotechnology. Stem Cell Research and Therapy 10 (1) : 38. ScholarBank@NUS Repository. https://doi.org/10.1186/s13287-019-1141-0Test; https://scholarbank.nus.edu.sg/handle/10635/178049Test
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