المؤلفون: Zequan Huang, Dong Liu, Shang Chen, Jinwei Ren, Chenghai Gao, Zhiyong Li, Aili Fan, Wenhan Lin

المصدر: Marine Drugs, Vol 22, Iss 2, p 78 (2024)

مصطلحات موضوعية: fungus, Spiromastix sp., brominated depsidone, antibacterial effects, Biology (General), QH301-705.5

الوصف: Eleven new brominated depsidones, namely spiromastixones U-Z5 (1–11) along with five known analogues (12–16), were isolated from a deep-sea-derived fungus Spiromastix sp. through the addition of sodium bromide during fermentation. Their structures were elucidated by extensive analysis of the spectroscopic data including high-resolution MS and 1D and 2D NMR data. Compounds 6–10 and 16 exhibited significant inhibition against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecium (VRE) with MIC values ranging from 0.5 to 2.0 μM. Particularly, tribrominated 7 displayed the strongest activity against MRSA and VRE with a MIC of 0.5 and 1.0 μM, respectively, suggesting its potential for further development as a new antibacterial agent.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1660-3397/22/2/78Test; https://doaj.org/toc/1660-3397Test

الوصول الحر: https://doaj.org/article/619463815c744a3eb2bde063c131b7b3Test

المؤلفون: Cunlei Cai (14298949), Yingying Chen (500738), Le Zhou (173199), Naying Gong (14230985), Hua Zhang (12549), Changli Sun (5522477), Junying Ma (466656), Jianhua Ju (773073)

مصطلحات موضوعية: Biochemistry, Microbiology, Pharmacology, Ecology, Infectious Diseases, Virology, Environmental Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, resistant bacterial strains, mic values ranging, hresims data sets, herein providing insights, staphylococcus aureus <, enterococcus faecalis <, bacillus subtilis <, racemates (< b, cyclopentenone (< b, atropisomers (< b, observed antimicrobial activities, antimicrobial drug development, 3 , 2 , various pathogenic bacteria, ray diffraction analyses, 4 , spiromastix <, antimicrobial polyketides, positive bacteria, chiral analyses, 4 μg

الوصف: Three diphenyl ethers ( 1 – 3 ) and a cyclopentenone ( 4 ), together with seven known compounds ( 5 – 11 ), were isolated from the fermentation broth of the marine sediment-derived fungus Spiromastix sp. SCSIO F190. Compounds 3 and 4 were found to exist as a pair of atropisomers ( 3a , 3b ) and racemates ( 4a , 4b ), respectively. The planar structures of compounds 1 – 4 were elucidated on the basis of NMR and HRESIMS data sets. The absolute configurations of 2 and 3 were determined by spectroscopic and single-crystal X-ray diffraction analyses, whereas the configuration of 4 was determined by spectroscopic and chiral analyses. All compounds, except for 4 and 11 , displayed activities against various pathogenic bacteria. Notably, compounds 1 – 4 , especially 1 , exhibited strong activity against Gram-positive bacteria, including methicillin-resistant bacterial strains of Staphylococcus aureus (MRSA), Enterococcus faecalis ATCC 29212, and Bacillus subtilis BS01, with MIC values ranging from 0.5 to 4 μg/mL. Moreover, the structure–activity relationship analyses of the active compounds and their analogues revealed the critical structural features correlating to the observed antimicrobial activities, herein providing insights for antimicrobial drug development.

العلاقة: https://figshare.com/articles/journal_contribution/Antimicrobial_Polyketides_from_the_Marine-Derived_Fungus_i_Spiromastix_i_sp_SCSIO_F190/21777340Test

الإتاحة: https://doi.org/10.1021/acs.jnatprod.2c00900.s002Test

المؤلفون: Yingying Chen, Cunlei Cai, Jiafan Yang, Junjie Shi, Yongxiang Song, Dan Hu, Junying Ma, Jianhua Ju

المصدر: Journal of Fungi; Volume 8; Issue 7; Pages: 715

مصطلحات موضوعية: marine-derived fungi, CRISPR-Cas9, Spiromastix sp. SCSIO F190, Aspergillus sp. SCSIO SX7S7, secondary metabolites, histone deacetylase

جغرافية الموضوع: agris

الوصف: Marine-derived fungi are emerging as attractive producers of structurally novel secondary metabolites with diverse bioactivities. However, the lack of efficient genetic tools limits the discovery of novel compounds and the elucidation of biosynthesis mechanisms. Here, we firstly established an effective PEG-mediated chemical transformation system for protoplasts in two marine-derived fungi, Spiromastix sp. SCSIO F190 and Aspergillus sp. SCSIO SX7S7. Next, we developed a simple and versatile CRISPR-Cas9-based gene disruption strategy by transforming a target fungus with a single plasmid. We found that the transformation with a circular plasmid encoding cas9, a single-guide RNA (sgRNA), and a selectable marker resulted in a high frequency of targeted and insertional gene mutations in both marine-derived fungal strains. In addition, the histone deacetylase gene rpd3 was mutated using the established CRISPR-Cas9 system, thereby activating novel secondary metabolites that were not produced in the wild-type strain. Taken together, a versatile CRISPR-Cas9-based gene disruption method was established, which will promote the discovery of novel natural products and further biological studies.

وصف الملف: application/pdf

العلاقة: https://dx.doi.org/10.3390/jof8070715Test

الإتاحة: https://doi.org/10.3390/jof8070715Test

المؤلفون: Amila Agampodi Dewa, Zeinab G. Khalil, Ahmed H. Elbanna, Robert J. Capon

المصدر: Molecules; Volume 27; Issue 10; Pages: 3172

مصطلحات موضوعية: chrysosporazine, brasiliamide, phenylpropanoid piperazine, P-glycoprotein inhibitor, fungal natural product, Chrysosporium, Aspergillus, Spiromastix, biodiscovery

جغرافية الموضوع: agris

الوصف: A library of fungi previously recovered from the gastrointestinal tract (GIT) of several fresh, commercially sourced Australian mullet fish was re-profiled for production of a rare class of phenylpropanoid piperazine alkaloids (chrysosporazines) using an integrated platform of; (i) miniaturized 24-well plate cultivation profiling (MATRIX), (ii) UPLC-DAD and UPLC-QTOF-MS/MS (GNPS) chemical profiling, and; (iii) precursor directed biosynthesis to manipulate in situ biosynthetic performance and outputs; to detect two new fungal producers of chrysosporazines. Chemical analysis of an optimized PDA solid phase cultivation of Aspergillus sp. CMB-F661 yielded the new regioisomeric chrysosporazine T (1) and U (2), while precursor directed cultivation amplified production and yielded the very minor new natural products azachrysosporazine T1 (3) and U1 (4), and the new unnatural analogues neochrysosporazine R (5) and S (6). Likewise, chemical analysis of an optimized M1 solid phase cultivation of Spiromastix sp. CMB-F455 lead to the GNPS detection of multiple chrysosporazines and brasiliamides, and the isolation and structure elucidation of chrysosporazine D (7) and brasiliamide A (8). Access to new chrysosporazine regioisomers facilitated structure activity relationship investigations to better define the chrysosporazine P-glycoprotein (P-gp) inhibitory pharmacophore, which is exceptionally potent at reversing doxorubrin resistance in P-gp over expressing colon carcinoma cells (SW600 Ad300).

وصف الملف: application/pdf

العلاقة: Natural Products Chemistry; https://dx.doi.org/10.3390/molecules27103172Test

الإتاحة: https://doi.org/10.3390/molecules27103172Test

المؤلفون: Xiang Guo (223678), Qinyu Meng (11015308), Siwen Niu (1816708), Jie Liu (15128), Xingchen Guo (11015311), Zhaolun Sun (11015314), Dong Liu (115204), Yucheng Gu (1670197), Jian Huang (7250), Aili Fan (2236705), Wenhan Lin (471132)

مصطلحات موضوعية: Molecular Biology, Immunology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Epigenetic Manipulation, SAHA, sediment-derived Spiromastix sp, TNF -α dose-dependently, terpene-related biosynthetic gene c., anti-neuroinflammatory activity, suberoylanilide hydroxamic acid, Antineuroinflammatory Effects Epige., Trigger Production, configurational assignments, spectroscopic techniques, COX -2, ECD, BV -2 cells, transcription levels, mRNA levels, LPS-induced BV -2, Guaiane-Type Sesquiterpenes, SAR analyses, multifunctional agent, BV -2 microglial cells, spiromaterpenes A-I, chemical conversion, LPS-induced NF -κB translocation, guaiane-type sesquiterpenoids

الوصف: Epigenetic manipulation of a deep-sea sediment-derived Spiromastix sp. fungus using suberoylanilide hydroxamic acid (SAHA) induction resulted in the activation of a terpene-related biosynthetic gene cluster, and nine new guaiane-type sesquiterpenes, spiromaterpenes A-I ( 1 – 9 ), were isolated. Their structures were determined using various spectroscopic techniques, in association with the modified Mosher’s method, computed electronic circular dichroism (ECD) spectra, and chemical conversion for configurational assignments. Compounds 4 – 6 exhibited significant effects against the NO production on lipopolysaccharide (LPS)-induced microglia cells BV2, and the preliminary SAR analyses demonstrated that a 2­( R ),11-diol unit is favorable. The most active 5 abolished LPS-induced NF-κB translocation from the cytosol to the nucleus in BV-2 microglial cells, accompanied by the marked reduction of the transcription levels of pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α dose-dependently in both LPS-induced BV-2 and BV-2 cells, as well as the protein and mRNA levels of iNOS and COX-2. This study complements the gap in knowledge regarding the anti-neuroinflammatory activity of guaiane-type sesquiterpenoids at the cellular level and suggests that 5 is promising for further optimization as a multifunctional agent for antineuroinflammation.

العلاقة: https://figshare.com/articles/journal_contribution/Epigenetic_Manipulation_to_Trigger_Production_of_Guaiane-Type_Sesquiterpenes_from_a_Marine-Derived_i_Spiromastix_i_sp_Fungus_with_Antineuroinflammatory_Effects/14832268Test

الإتاحة: https://doi.org/10.1021/acs.jnatprod.1c00293.s001Test

المؤلفون: Chongming Wu, Ran Chen, Mingyue Liu, Dong Liu, Xin Li, Shuai Wang, Siwen Niu, Peng Guo, Wenhan Lin

المصدر: Marine Drugs, Vol 13, Iss 10, Pp 6352-6365 (2015)

مصطلحات موضوعية: spiromastixones, Spiromastix sp., atherosclerosis, foam cell, PPARγ, ABCA1/G1, CD36, Biology (General), QH301-705.5

الوصف: Bioassay-guided evaluation shows that a deep sea-derived fungus, Spiromastix sp. MCCC 3A00308, possesses lipid-lowering activity. Chromatographic separation of a culture broth resulted in the isolation of 15 known depsidone-based analogues, labeled spiromastixones A–O (1–15). Each of these compounds was tested for its ability to inhibit oxidized low-density lipoprotein (oxLDL)-induced foam cell formation in RAW264.7 macrophages. Spiromastixones 6–8 and 12–14 significantly decreased oxLDL-induced lipid over-accumulation, reduced cell surface area, and reduced intracellular cholesterol concentration. Of these compounds, spiromastixones 6 and 14 exerted the strongest inhibitory effects. Spiromastixones 6 and 14 dramatically inhibited cholesterol uptake and stimulated cholesterol efflux to apolipoprotein A1 (ApoA1) and high-density lipoprotein (HDL) in RAW264.7 macrophages. Mechanistic investigation indicated that spiromastixones 6, 7, 12 and 14 significantly up-regulated the mRNA levels of ATP-binding cassette sub-family A1 (ABCA1) and down-regulated those of scavenger receptor CD36, while the transcription of ATP-binding cassette sub-family A1 (ABCG1) and proliferator-activated receptor gamma (PPARγ) were selectively up-regulated by 6 and 14. A transactivation reporter assay revealed that spiromastixones 6 and 14 remarkably enhanced the transcriptional activity of PPARγ. These results suggest that spiromastixones inhibit foam cell formation through upregulation of PPARγ and ABCA1/G1 and downregulation of CD36, indicating that spiromastixones 6 and 14 are promising lead compounds for further development as anti-atherogenic agents.

وصف الملف: electronic resource

العلاقة: http://www.mdpi.com/1660-3397/13/10/6352Test; https://doaj.org/toc/1660-3397Test

الوصول الحر: https://doaj.org/article/561b6f719ac04ceba4887ac9677d57b1Test

المؤلفون: Siwen Niu, Dong Liu, Peter Proksch, Zongze Shao, Wenhan Lin

المصدر: Marine Drugs, Vol 13, Iss 4, Pp 2526-2540 (2015)

مصطلحات موضوعية: fungus from deep sea, Spiromastix sp., spiromastols A–K, polyphenols, antibacterial activities, Biology (General), QH301-705.5

الوصف: Eleven new polyphenols namely spiromastols A–K (1–11) were isolated from the fermentation broth of a deep sea-derived fungus Spiromastix sp. MCCC 3A00308. Their structures were determined by extensive NMR data and mass spectroscopic analysis in association with chemical conversion. The structures are classified as diphenyl ethers, diphenyl esters and isocoumarin derivatives, while the n-propyl group in the analogues is rarely found in natural products. Compounds 1–3 exhibited potent inhibitory effects against a panel of bacterial strains, including Xanthomanes vesicatoria, Pseudomonas lachrymans, Agrobacterium tumefaciens, Ralstonia solanacearum, Bacillus thuringensis, Staphylococcus aureus and Bacillus subtilis, with minimal inhibitory concentration (MIC) values ranging from 0.25 to 4 µg/mL. The structure-activity relationships are discussed, while the polychlorinated analogues 1–3 are assumed to be a promising structural model for further development as antibacterial agents.

وصف الملف: electronic resource

العلاقة: http://www.mdpi.com/1660-3397/13/4/2526Test; https://doaj.org/toc/1660-3397Test

الوصول الحر: https://doaj.org/article/9dfdf860b0784a3dbb79a1644b9f1cdcTest

المؤلفون: Jiang Huang, Siwen Niu, Zongze Shao, Dong Liu, Aili Fan, Wenhan Lin

المصدر: RSC Advances. 11:29661-29667

مصطلحات موضوعية: biology, Chemistry, Spiromastix, General Chemical Engineering, Pathogenic bacteria, General Chemistry, Fungus, Bacillus subtilis, biology.organism_classification, medicine.disease_cause, Chromatographic separation, Minimum inhibitory concentration, Staphylococcus aureus, Bacillus thuringiensis, medicine, Food science

الوصف: Chromatographic separation of the solid cultures of a deep-sea-derived Spiromastix fungus (MCCC 3A00308) resulted in the isolation of eight compounds. Their structures were identified on the basis of the spectroscopic data. Compounds 1–8 are classified as depsidone-type (1–4), isocoumarin-type (5 and 6), and benzothiazole-type (7 and 8), of which 1–7 are new compounds and 1–3 along with 5 and 6 are chlorinated. Compound 3 is characterized by trichlorination and shows potent activities against Gram-positive pathogenic bacteria including Staphylococcus aureus ATCC 25923, Bacillus thuringiensis ATCC 10792, and Bacillus subtilis CMCC 63501, with minimum inhibitory concentration (MIC) values ranging from 0.5 to 1.0 μg mL−1. This study extends the chemical diversity of chlorinated natural products from marine-derived fungi and provides a promising lead for the development of antibacterial agents.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::db64604ca74e774a4143da8bc661b5d8Test
https://doi.org/10.1039/d1ra05736gTest

المؤلفون: Siwen Niu, Dong Liu, Peter Proksch, Zongze Shao, Wenhan Lin

المساهمون: The Pennsylvania State University CiteSeerX Archives

المصدر: http://www.mdpi.com/1660-3397/13/4/2526/pdfTest/.

مصطلحات موضوعية: fungus from deep sea, Spiromastix sp, spiromastols A–K, polyphenols, antibacterial activities

الوصف: marine drugs

العلاقة: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.699.1Test; http://www.mdpi.com/1660-3397/13/4/2526/pdfTest/

الإتاحة: http://www.mdpi.com/1660-3397/13/4/2526/pdfTest/

المؤلفون: Josep Guarro, Nathan P. Wiederhold, Alberto M. Stchigel, Deanna A. Sutton, José F. Cano-Lira

المصدر: Mycopathologia. 182:967-978

مصطلحات موضوعية: 0301 basic medicine, Biopsy, Veterinary (miscellaneous), 030106 microbiology, Gymnoascus, Applied Microbiology and Biotechnology, Microbiology, Conidium, 03 medical and health sciences, Botany, Humans, DNA, Fungal, Lung, Phylogeny, biology, Ascomycota, Spiromastix, Onygenales, Spores, Fungal, Ribosomal RNA, biology.organism_classification, Mycoses, Eurotiomycetes, RNA, Ribosomal, Taxonomy (biology), Agronomy and Crop Science

الوصف: The new species Spiromastigoides albida (Onygenales, Eurotiomycetes, Ascomycota), from a lung biopsy in USA, is proposed and described based on morphological data and the analysis of rRNA, and fragments of actin and ß-tubulin gene sequences. This species is characterized by white colonies and a malbranchea-like asexual morph with profusely branching curved conidiophores forming sporodochia-like structures. Moreover, new combinations for Gymnoascus alatosporus, and for some new species recently described under the generic name Spiromastix, are provided.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::786aee89530bc62aae75929f729711a6Test
https://doi.org/10.1007/s11046-017-0179-8Test