المؤلفون: Phanvasri Saengsuwan, Kamonnut Singkhamanan, Siribhorn Madla, Natnicha Ingviya, Chonticha Romyasamit

المصدر: PeerJ, Vol 9, p e11478 (2021)

مصطلحات موضوعية: Antibiotic resistance, Enterococci, Multiplex PCR, Van genes, Virulence determinants, Medicine, Biology (General), QH301-705.5

الوصف: Objective Vancomycin-resistant enterococci are nosocomial pathogens that are responsible for commonly causing healthcare-associated infections, and they exhibit increased resistance to many antimicrobials, particularly to vancomycin. The epidemiological data available on vancomycin-resistant enterococci (VRE) in Thailand are inadequate. Methods Using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), this study investigated genes that encode antimicrobial resistance and genetic relatedness to further understand VRE prevalence. Ninety VRE isolates were collected between 2011 and 2019 from a tertiary care hospital in southern Thailand. Antimicrobial susceptibility was determined using the disk diffusion method and E-test methods. Multiplex PCR was performed to detect the van gene and virulence genes. Results The study showed a high prevalence of diverse multidrug-resistant VRE strains. The prevalence of VRE infection was the highest in 2014 (28 isolates, 39.4%). VRE were mostly found in the urogenital tract (26 isolates, 28.9%), followed by the digestive tract (20%), body fluid, i.e., pancreatic cyst fluid, peritoneal dialysis fluid, Jackson–Pratt (JP) drain (20%), and blood specimens (10%). Patients in medical and surgical wards had 71.1% multi-drug-resistant and 28.9% extensively drug-resistant (XDR) VRE strains, respectively. The most prevalent antibiotic resistance was to ampicillin (74.4%). Susceptibility to gentamicin and meropenem were similar (7% and 10%, respectively). Four isolates (4.4%) were resistant to colistin. Only vanA was detected among the strains. The virulence gene test showed that the detection rates of enterococcal surface protein (esp) and hyaluronidase (hyl) genes were 91.1% and 5.6%, respectively. According to ERIC-PCR analysis, 51 of 90 strains had clonality, with a similarity rate of 95%. Conclusions We conclude that there is a need to implement infection control practices and active surveillance. Molecular techniques can effectively detect antibiotic-resistant genes, which would allow monitoring to control VRE infection in hospitals.

وصف الملف: electronic resource

العلاقة: https://peerj.com/articles/11478.pdfTest; https://peerj.com/articles/11478Test/; https://doaj.org/toc/2167-8359Test

الوصول الحر: https://doaj.org/article/90ec0d13182647a8a1b1a573124d8e62Test

المؤلفون: Siribhorn Madla, Potchanapond Graidist

المصدر: Journal of Health Science and Medical Research (JHSMR), Vol 35, Iss 1, Pp 83-94 (2017)

مصطلحات موضوعية: alkaloids, anticancer activity, anticancer mechanism, cancer, multidrug resistance, Medicine

الوصف: Multidrug resistance is one of the major obstacles to successful cancer chemotherapy, which causes cancer to remain one of the leading causes of death worldwide. Therefore, the use of natural products for cancer prevention and therapy has been continuously investigated and developed for decades. Alkaloids are one of the important compounds derived from natural products that have been extensively studied for their potential use as cancer chemopreventive and chemotherapeutic drugs in both in vitro and animal studies. This short review intends to present the molecular mechanisms of action of alkaloids on stimulation and inhibition some signaling molecules involved in cell proliferation, differentiation, angiogenesis, metastasis and apoptosis pathways of cancer cells. The understanding of the molecular mechanisms of alkaloids against cancer will provide clues towards a diagnostic, prevention and therapeutic applications. Based on the information provided in this article, alkaloids show remarkable potential as anticancer agents. However, more research and clinical trials are crucial before applying any of these alkaloids as new medicine to treat cancer patients.

وصف الملف: electronic resource

العلاقة: https://www.jhsmr.org/index.php/jhsmr/article/view/135Test; https://doaj.org/toc/2586-9981Test; https://doaj.org/toc/2630-0559Test

الوصول الحر: https://doaj.org/article/194fdcf68b1a4836827d8ca63affa89eTest

المؤلفون: Daisuke MIURA, Hiroyuki WARIISHI, Siribhorn MADLA

المصدر: Analytical Sciences. 2012, 28(3):301

المؤلفون: Siribhorn Madla, Yuan-E Lee, Prema, Hiroyuki Morita, Abdi Wira Septama, Amit Jaisi

المصدر: Chemistrybiodiversity. 18(12)

مصطلحات موضوعية: Plumbago indica, Caesalpinia sappan, viruses, Bioengineering, Biochemistry, chemistry.chemical_compound, Humans, Medicinal plants, Molecular Biology, Punicalagin, Centella, Plants, Medicinal, biology, Traditional medicine, Molecular Structure, Chemistry, Plant Extracts, food and beverages, Alpinia, General Chemistry, General Medicine, vpr Gene Products, Human Immunodeficiency Virus, biology.organism_classification, Thailand, Hydrolyzable Tannins, Triterpenes, Rhinacanthus nasutus, Zingiber cassumunar, Molecular Medicine, HeLa Cells

الوصف: Viral protein R (Vpr) is an accessory protein in Human immunodeficiency virus-1 (HIV-1) and has been suggested as an attractive target for HIV disease treatment. Investigations of the ethanolic extracts of twelve Thai herbs revealed that the extracts of the Punica granatum fruits, the Centella asiatica aerials, the Citrus hystrix fruit peels, the Caesalpinia sappan heartwoods, the Piper betel leaves, the Alpinia galangal rhizomes, the Senna tora seeds, the Zingiber cassumunar rhizomes, the Rhinacanthus nasutus leaves, and the Plumbago indica roots exhibited the anti-Vpr activity in HeLa cells harboring the TREx plasmid encoding full-length Vpr (TREx-HeLa-Vpr cells). Moreover, the investigation of the selected main constituents in Punica granatum, Centella asiatica, A. galangal, and Caesalpinia sappan indicated that punicalagin, asiaticoside, ellagic acid, madecassic acid, madecassoside, zingerone, brazilin, and asiatic acid possessed anti-Vpr activities at the 10 μM concentration. Among the tested extracts and compounds, the extracts from Centella asiatica and Citrus hystrix and the compounds, punicalagin and asiaticoside, showed the most potent anti-Vpr activities without any cytotoxicity, respectively.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::92efcd4a21b0410324d57ca4bf6dfc8fTest
https://pubmed.ncbi.nlm.nih.gov/34599555Test

المؤلفون: Kamonnut Singkhamanan, Natnicha Ingviya, Siribhorn Madla, Phanvasri Saengsuwan, Chonticha Romyasamit

المصدر: PeerJ, Vol 9, p e11478 (2021)
PeerJ

مصطلحات موضوعية: Epidemiology, Antibiotic resistance, Enterococci, Biochemistry, Microbiology, Meropenem, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, 0302 clinical medicine, Ampicillin, medicine, Infection control, 030212 general & internal medicine, Van genes, 0303 health sciences, Virulence determinants, Molecular epidemiology, 030306 microbiology, business.industry, General Neuroscience, General Medicine, biochemical phenomena, metabolism, and nutrition, Multiplex PCR, bacterial infections and mycoses, Infectious Diseases, Colistin, Vancomycin, Medicine, Gentamicin, Public Health, General Agricultural and Biological Sciences, business, medicine.drug

الوصف: Objective Vancomycin-resistant enterococci are nosocomial pathogens that are responsible for commonly causing healthcare-associated infections, and they exhibit increased resistance to many antimicrobials, particularly to vancomycin. The epidemiological data available on vancomycin-resistant enterococci (VRE) in Thailand are inadequate. Methods Using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), this study investigated genes that encode antimicrobial resistance and genetic relatedness to further understand VRE prevalence. Ninety VRE isolates were collected between 2011 and 2019 from a tertiary care hospital in southern Thailand. Antimicrobial susceptibility was determined using the disk diffusion method and E-test methods. Multiplex PCR was performed to detect the van gene and virulence genes. Results The study showed a high prevalence of diverse multidrug-resistant VRE strains. The prevalence of VRE infection was the highest in 2014 (28 isolates, 39.4%). VRE were mostly found in the urogenital tract (26 isolates, 28.9%), followed by the digestive tract (20%), body fluid, i.e., pancreatic cyst fluid, peritoneal dialysis fluid, Jackson–Pratt (JP) drain (20%), and blood specimens (10%). Patients in medical and surgical wards had 71.1% multi-drug-resistant and 28.9% extensively drug-resistant (XDR) VRE strains, respectively. The most prevalent antibiotic resistance was to ampicillin (74.4%). Susceptibility to gentamicin and meropenem were similar (7% and 10%, respectively). Four isolates (4.4%) were resistant to colistin. Only vanA was detected among the strains. The virulence gene test showed that the detection rates of enterococcal surface protein (esp) and hyaluronidase (hyl) genes were 91.1% and 5.6%, respectively. According to ERIC-PCR analysis, 51 of 90 strains had clonality, with a similarity rate of 95%. Conclusions We conclude that there is a need to implement infection control practices and active surveillance. Molecular techniques can effectively detect antibiotic-resistant genes, which would allow monitoring to control VRE infection in hospitals.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20b586c63d10e5b4ac7ae55bae924f49Test
https://peerj.com/articles/11478Test/

المؤلفون: Potchanapond Graidist, Theera Srisawat, Yaowapa Sukpondma, Siribhorn Madla, Somchai Sriwiriyajan

المصدر: Biomedicine & Pharmacotherapy. 92:732-743

مصطلحات موضوعية: 0301 basic medicine, Cell, Apoptosis, Breast Neoplasms, Cell Cycle Proteins, Pharmacology, Biology, Lignans, Piperlonguminine, Flow cytometry, 03 medical and health sciences, 0302 clinical medicine, Cell Line, Tumor, medicine, Humans, Cytotoxic T cell, Cytotoxicity, medicine.diagnostic_test, Cell Cycle, Dioxolanes, Cell Cycle Checkpoints, General Medicine, Cell cycle, 030104 developmental biology, medicine.anatomical_structure, Cell culture, 030220 oncology & carcinogenesis, Female, Piper nigrum, Densitometry, Phytotherapy

الوصف: Several studies have reported that active compounds isolated from Piper nigrum possess anticancer properties. However, there are no data on anticancer activity of (-)-kusunokinin and piperlonguminine. The purposes of this study were to isolate active compounds from P. nigrum and identify the molecular mechanisms underlying growth and apoptosis pathway in breast cancer cells. Two bioactive compounds, (-)-kusunokinin and piperlonguminine, were isolated from P. nigrum. Cytotoxicity and the molecular mechanism were measured by methyl thiazolyl tetrazolium (MTT) assay, flow cytometry and Western blot analysis. We found that the active compounds, which effect cancer cell lines were (-)-kusunokinin and piperlonguminine. These compounds have potent cytotoxic effects on breast cancer cells (MCF-7 and MDA-MB-468) and colorectal cells (SW-620). (-)-Kusunokinin demonstrated a cytotoxic effect on MCF-7 and MDA-MB-468 with IC50 values of 1.18 and 1.62μg/mL, respectively. Piperlonguminine had a cytotoxic effect on MCF-7 and MDA-MB-468 with IC50 values of 1.63 and 2.19μg/mL, respectively. Both compounds demonstrated lower cytotoxicity against normal breast cell lines with IC50 values higher than 11μg/mL. Cell cycle and apoptotic analysis using flow cytometry, showed that the (-)-kusunokinin and piperlonguminine induced cell undergoing apoptosis and drove cells towards the G2/M phase. Moreover, both compounds decreased topoisomerase II and bcl-2. The increasing of p53 levels further increased p21, bax, cytochrome c, caspase-8, -7 and -3 activities, except caspase-9. These results suggest that the (-)-kusunokinin and piperlonguminine have been shown to have potent anticancer activities through extrinsic pathway and G2/M phase arrest.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e69aef4c99da122298a62000b0b644d7Test
https://doi.org/10.1016/j.biopha.2017.05.130Test

المؤلفون: Thidarath Rattanaburee, Somchai Sriwiriyajan, Sirinapa Dokduang, Varomyalin Tipmanee, Potchanapond Graidist, Siribhorn Madla, Yaowapa Sukpondma, Narissara Lailerd, Aman Tedasen

المصدر: European Journal of Pharmacology. 882:173311

مصطلحات موضوعية: 0301 basic medicine, Cell Survival, Estrogen receptor, Antineoplastic Agents, Lignans, Metastasis, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, Breast cancer, Cell Movement, Cell Line, Tumor, medicine, Animals, Humans, Pharmacology, Mammary tumor, Cell growth, Chemistry, Cell Cycle, Mammary Neoplasms, Experimental, Cancer, Methylnitrosourea, Cell cycle, medicine.disease, 030104 developmental biology, Cancer cell, Cancer research, Female, 030217 neurology & neurosurgery

الوصف: Natural and synthetic (−)-kusunokinin inhibited breast cancer, colon cancer and cholangiocarcinoma cells at the G2/M phase and induced apoptosis. However, there is no report on the action and adverse effects of (−)-kusunokinin in animal models. In this study, we investigated the cytotoxic effect of (−)-kusunokinin from Piper nigrum on cancer cells. NMU-induced rat mammary tumors, an ER positive breast cancer model, were treated with (−)-kusunokinin. Proteins of interest related to cell cycle, angiogenesis, migration and signaling proteins were detected in tumor tissues. Results showed that (−)-kusunokinin exhibited strong cytotoxicity against breast, colon and lung cancer cells and caused low toxicity against normal fibroblast cells. For in vivo study, 7.0 mg/kg and 14.0 mg/kg of (−)-kusunokinin reduced tumor growth without side effects on body weight, internal organs and bone marrow. Combination of (−)-kusunokinin with a low effective dose of doxorubicin significantly inhibited tumor growth and provoked cell death in cancer tissues. Mechanistically, 14.0 mg/kg of (−)-kusunokinin decreased cell proliferation (c-Src, PI3K, Akt, p-Erk1/2 and c-Myc), cell cycle (E2f-1, cyclin B1 and CDK1), and metastasis (E-cadherin, MMP-2 and MMP-9) proteins in tumor tissues, which supports its anticancer effect. We further confirmed the antimigration effect of (−)-kusunokinin; the results show that this compound inhibited breast cancer cell (MCF-7) migration in a dose-dependent manner. In conclusion, the results suggest that 14 mg/kg of (−)-kusunokinin inhibited tumors through the reduction of signaling proteins and their downstream molecules. Therefore, (−)-kusunokinin becomes an intriguing candidate for cancer treatment as it provides a strong potency in cancer inhibition.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b8a5364873374aca1e5ec03b9c1fb81Test
https://doi.org/10.1016/j.ejphar.2020.173311Test

المؤلفون: Siribhorn Madla, Aman Tedasen, Araya Khoka, Somchai Sriwiriyajan, Potchanapond Graidist

المصدر: Pharmacognosy Magazine. 16:28

مصطلحات موضوعية: biology, medicine.diagnostic_test, Cell growth, Pharmaceutical Science, 01 natural sciences, Molecular biology, 0104 chemical sciences, 010404 medicinal & biomolecular chemistry, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, chemistry, Western blot, Cell culture, Apoptosis, 030220 oncology & carcinogenesis, Piperine, Drug Discovery, biology.protein, medicine, DNA fragmentation, p53 upregulated modulator of apoptosis, Fragmentation (cell biology)

الوصف: Background: Black pepper (Piper nigrum L.) is widely used as a traditional medicine, including usage for pain relief, fevers, as well as an anticancer agent. Previously, we reported that piperine-free P. nigrum extract (PFPE) inhibited breast cancer in vitro and in vivo. Objective: In this present study, we explored the anticancer effects of PFPE on cholangiocarcinoma (CCA). Materials and Methods: 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to analyze cytotoxic potential of PFPE whereas deoxyribonucleic acid (DNA) fragmentation followed by Western blot analysis were used. Results: PFPE composed of alkaloid, flavonoid, amide, lignans, opioid, and steroid. This crude extract represented cytotoxic effect against CCA cells which stronger than dichloromethane P. nigrum crude extract and piperine, especially on KKU-M213 (median inhibition concentration [IC50] at 13.70 μg/ml) and TFK-1 (IC50at 15.30 μg/ml). Interestingly, PFPE showed lower cytotoxicity against normal human cholangiocyte MMNK-1 cells (IC50at 19.65 μg/ml) than KKU-M213 and TFK-1 cells. Then, the molecular mechanisms of PFPE were firstly evaluated by DNA fragmentation followed by Western blot analysis. The degradation of DNA was observed on KKU-M213 and TFK-1 cells after treatment with PFPE at day 2. Then, proliferation proteins including topoisomerase II, AKT8 virus oncogene cellular homolog, avian myelocytomatosis virus oncogene cellular homolog, cyclin D1, signal transducer and activator of transcription 3, cyclooxygenase-2, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) were decreased and p21 was increased. Furthermore, apoptotic proteins, such as tumor protein p53, Bcl-2-associated X protein, and p53 upregulated modulator of apoptosis were upregulated. Meanwhile, antiapoptotic protein B-cell lymphoma 2 was down-regulated. Conclusion: These results indicated that PFPE inhibited CCA through the down-regulation of cell proliferation and induction of apoptosis pathway.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::0179486187bea0a6c126ff5befd3c55aTest
https://doi.org/10.4103/pm.pm_288_19Test

المؤلفون: Santad Wichienchot, Potchanapond Graidist, Knud Erik Bach Knudsen, Vijitra Plongbunjong, Siribhorn Madla, Pleumjit Bunyapipat

المصدر: Graidist, P, Plongbunjong, V, Wichienchot, S, Madla, S, Bunyapipat, P & Knudsen, K E B 2019, ' Isomalto-oligosaccharides from rice and their potential use as pharma-nutraceuticals in prevention of colon cancer ', Functional Foods in Health and Disease, vol. 9, no. 6, pp. 371-383 . https://doi.org/10.31989/ffhd.v9i6.598Test
Functional Foods in Health and Disease, Vol 9, Iss 6, Pp 494-507 (2019)

مصطلحات موضوعية: 0301 basic medicine, Colorectal cancer, medicine.medical_treatment, 030106 microbiology, Medicine (miscellaneous), lcsh:TX341-641, Pharmacology, Gut flora, Biochemistry, 03 medical and health sciences, In vivo, Oral administration, medicine, isomalto-oligosaccharides, Carcinogen, lcsh:R5-920, gut modulation, Nutrition and Dietetics, biology, business.industry, rice, Prebiotic, medicine.disease, biology.organism_classification, Acute toxicity, 030104 developmental biology, colon cancer, prebiotic, Brown rice, lcsh:Medicine (General), business, lcsh:Nutrition. Foods and food supply, Food Science

الوصف: Background: Isomalto-oligosaccharides (IMO) were enzymatically produced from rice (rIMO) alone or in combination with product (instant rice porridge mixed rIMO) demonstrated bifidogenic and butyrogenic effects in in vitro studies. The potential use of IMO from rice for pharma-nutraceuticals to prevent or risk reduction of colon cancer was further investigated in rats in this study. Objective: To investigate potential use of IMO from rice on prevention or risk reduction of colon cancer and gut microbiota modulation in rats, colonic polyp formation, histological changes, gut microbiota modulation, and butyrogenic properties were evaluated. Methods: An acute toxicity test was performed in ICR mice with a single dose of 2,000 mg/kg of tested IMO. The effects of rIMO were performed in AOM/DSS-induced Wistar rats. The animals were divided in 8 groups and treated three times a week with vehicle, three doses of rIMO, cIMO, and product (instant rice porridge mixed rIMO) before and after carcinogen administration. Animals were sacrificed at the end of 19 th week. Results: Oral administration at up to 2,000 mg/kg of rIMO and product containing rIMO could be considered safe. Additionally, the animals chemically induced with AOM/DSS and treated with rIMO (1,500 mg/kg) and product containing rIMO significantly lower the occurrence of colonic polyps about 60%. There were no changes in other blood hematologic and biochemistry values but an improved gut barrier function when compared with animals in control and vehicle groups. AOM/DSS-induced rats supplemented with product containing rIMO could retard the reduction of beneficial bacteria and butyric acid production and thereby suppres the increase of harmful bacteria through the AOM/DSS post-induction phase. Conclusion: The results of this in vivo study suggest that consumption of rIMO alone or in combination with brown rice porridge could potentially help protect histological changes and risk reduction of developing for colon cancer. IMO from rice has potential use for pharma-nutraceuticals for risk reduction of colon cancer. Keywords: colon cancer, isomalto-oligosaccharides, prebiotic, rice, gut modulation

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::961bd1ea28eff6ed4f97749897eeb38fTest
https://doi.org/10.31989/ffhd.v9i6.598Test

المؤلفون: Vijitra Plongbunjong, Santad Wichienchot, Siribhorn Madla, Pleumjit Bunyapipat, Bach Knudsen, Knud Erik, Potchanapond Graidist

المصدر: Functional Foods in Health & Disease; Jun2019, Vol. 9 Issue 6, p371-383, 13p

مصطلحات موضوعية: COLON cancer, CANCER prevention, BROWN rice, GUT microbiome, ACUTE toxicity testing, RICE, PREBIOTICS

الشركة/الكيان: INTERNATIONAL Maritime Organization

مستخلص: Background: Isomalto-oligosaccharides (IMO) were enzymatically produced from rice (rIMO) alone or in combination with product (instant rice porridge mixed rIMO) demonstrated bifidogenic and butyrogenic effects in in vitro studies. The potential use of IMO from rice for pharma-nutraceuticals to prevent or risk reduction of colon cancer was further investigated in rats in this study. Objective: To investigate potential use of IMO from rice on prevention or risk reduction of colon cancer and gut microbiota modulation in rats, colonic polyp formation, histological changes, gut microbiota modulation, and butyrogenic properties were evaluated. Methods: An acute toxicity test was performed in ICR mice with a single dose of 2,000 mg/kg of tested IMO. The effects of rIMO were performed in AOM/DSS-induced Wistar rats. The animals were divided in 8 groups and treated three times a week with vehicle, three doses of rIMO, cIMO, and product (instant rice porridge mixed rIMO) before and after carcinogen administration. Animals were sacrificed at the end of 19^th week. Results: Oral administration at up to 2,000 mg/kg of rIMO and product containing rIMO could be considered safe. Additionally, the animals chemically induced with AOM/DSS and treated with rIMO (1,500 mg/kg) and product containing rIMO significantly lower the occurrence of colonic polyps about 60%. There were no changes in other blood hematologic and biochemistry values but an improved gut barrier function when compared with animals in control and vehicle groups. AOM/DSS-induced rats supplemented with product containing rIMO could retard the reduction of beneficial bacteria and butyric acid production and thereby suppres the increase of harmful bacteria through the AOM/DSS post-induction phase. Conclusion: The results of this in vivo study suggest that consumption of rIMO alone or in combination with brown rice porridge could potentially help protect histological changes and risk reduction of developing for colon cancer. IMO from rice has potential use for pharmanutraceuticals for risk reduction of colon cancer. [ABSTRACT FROM AUTHOR]

: Copyright of Functional Foods in Health & Disease is the property of Functional Foods in Health & Disease and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)