المؤلفون: Molfino, Alessio, Imbimbo, Giovanni, Amabile, Maria Ida, Ammann, Thomas, Lionetto, Luana, Salerno, Gerardo, Simmaco, Maurizio, Chiappini, Maria Grazia, Muscaritoli, Maurizio

المساهمون: Molfino, Alessio, Imbimbo, Giovanni, Amabile, Maria Ida, Ammann, Thoma, Lionetto, Luana, Salerno, Gerardo, Simmaco, Maurizio, Chiappini, Maria Grazia, Muscaritoli, Maurizio

مصطلحات موضوعية: IDO, Kyn/Trp ratio, body composition, dialysi, fatigue, inflammation, muscle mass

الوصف: Fatigue is a frequent symptom in hemodialysis (HD), and the indolamine-2,3-dioxygenase (IDO) metabolic trap has been hypothesized in the pathogenesis of fatigue. The association between IDO activity according to fatigue and its relationship with muscle mass and function in HD patients was verified. Chronic HD patients were considered, and fatigue was assessed. The plasma kynurenines and tryptophan ratio (Kyn/Trp), as surrogate of IDO activity, and interleukin (IL)-6 were measured. Muscularity was assessed by BIA and muscle strength by hand-grip dynamometer. 50 HD patients were enrolled, and fatigue was present in 24% of the cohort. Patients with fatigue showed higher Kyn/Trp (p = 0.005), were older (p = 0.007), and IL-6 levels resulted higher than in non-fatigue patients (p < 0.001). HD patients with fatigue showed lower intracellular water (surrogate of muscle mass) (p < 0.001), as well as lower hand grip strength (p = 0.02). The Kyn/Trp ratio positively correlated with IL-6 and ECW/ICW (p = 0.004 and p = 0.014). By logistic regression analysis, higher ICW/h(2) was associated with lower odds of fatigue (OR, 0.10; 95% CI, 0.01 to 0.73). In conclusion, our cohort fatigue was associated with a higher Kyn/Trp ratio, indicating a modulation of IDO activity. The Kyn/Trp ratio correlated with IL-6, suggesting a potential role of IDO and inflammation in inducing fatigue and changes in muscularity.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/36839234; info:eu-repo/semantics/altIdentifier/wos/WOS:000941703800001; volume:15; issue:4; numberofpages:11; journal:NUTRIENTS; https://hdl.handle.net/11573/1688751Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85148884292

الإتاحة: https://doi.org/10.3390/nu15040876Test
https://hdl.handle.net/11573/1688751Test

المؤلفون: Salerno, Gerardo, Borro, Marina, Visco, Vincenzo, Olana, Soraya, Gargano, Francesca, Raffa, Salvatore, Zamponi, Virginia, Mancini, Camilla, Faggiano, Antongiulio, Simmaco, Maurizio, Mazzilli, Rossella

المساهمون: Salerno, Gerardo, Borro, Marina, Visco, Vincenzo, Olana, Soraya, Gargano, Francesca, Raffa, Salvatore, Zamponi, Virginia, Mancini, Camilla, Faggiano, Antongiulio, Simmaco, Maurizio, Mazzilli, Rossella

مصطلحات موضوعية: cytokine, inflammation, interleukin, male infertility, medications

الوصف: Background and Objectives: The aim of this study was to evaluate the impact of medications on oxidative stress, inflammatory biomarkers and semen characteristics in males with idiopathic infertility. Materials and Methods: In this observational case-control clinical study, 50 men with idiopathic infertility were enrolled, of whom 38 (the study group) were on pharmacological treatment and 12 made up the control group. The study group was clustered according to the medications (Group A: anti-hypertensive, n = 10; Group B: thyroxine, n = 6; Group C: non-steroidal anti-inflammatory drugs, n = 13; Group D: miscellaneous, n = 6; Group E: lipid-lowering drugs, n = 4). Semen analyses were performed according to WHO 2010 guidelines. Interleukins (IL)-10, IL-1 beta, IL-4, IL-6, Tumor Necrosis Factor- alpha (TNF-alpha) and IL-1 alpha were determined using a solid-phase sandwich immunoassay. The diacron reactive oxygen metabolites, d-ROMs test, was performed by means of a colorimetric determination of reactive oxygen metabolites and measured with a spectrophotometer. Beta-2-microglobulin and cystatin-C were measured with an immunoturbidimetric analyzer. Results: No differences between the study and control groups for age and macroscopic and microscopic semen characteristics were found, nor were any differences found after clustering according to the drug categories. IL-1 alpha and IL-10 were significantly lower in the study group compared with the control group; IL-10 was significantly lower in groups A, B, C and D compared with the control group. Furthermore, a direct correlation between IL-1 alpha, IL-10 and TNF-alpha and leukocytes was found. Conclusions: Despite the sample size limitations, the data suggest a correlation between drug use and activation of the inflammatory response. This could clarify the pathogenic mechanism of action for several pharmacological classes on male infertility.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/37241135; info:eu-repo/semantics/altIdentifier/wos/WOS:000997620300001; volume:59; issue:5; firstpage:1; lastpage:13; numberofpages:13; journal:MEDICINA; https://hdl.handle.net/11573/1694289Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85160218143

الإتاحة: https://doi.org/10.3390/medicina59050903Test
https://hdl.handle.net/11573/1694289Test

المؤلفون: Del Casale, Antonio, Simmaco, Maurizio, Modesti, Martina Nicole, Zocchi, Clarissa, Arena, Jan Francesco, Bilotta, Irene, Alcibiade, Alessandro, Sarli, Giuseppe, Cutillo, Lorenzo, Antonelli, Giulia, La Spina, Enrico, De Luca, Ottavia, Preissner, Robert, Borro, Marina, Gentile, Giovanna, Girardi, Paolo, Pompili, Maurizio

المساهمون: Del Casale, Antonio, Simmaco, Maurizio, Modesti, Martina Nicole, Zocchi, Clarissa, Arena, Jan Francesco, Bilotta, Irene, Alcibiade, Alessandro, Sarli, Giuseppe, Cutillo, Lorenzo, Antonelli, Giulia, La Spina, Enrico, De Luca, Ottavia, Preissner, Robert, Borro, Marina, Gentile, Giovanna, Girardi, Paolo, Pompili, Maurizio

مصطلحات موضوعية: 5-hydroxytryptamine receptor, antipsychotic agent, dopamine receptor, drug resistance, pharmacogenomic variant, precision medicine

الوصف: Background: The objective of this study was to investigate the DRD2 rs1800497, rs1799732, rs1801028, DRD3 rs6280, and HTR2A rs6314, rs7997012, and rs6311 single-nucleotide polymorphism (SNP) correlations with resistance to second-generation antipsychotics (SGAs) in a real-world sample of patients with treatment-resistant mental disorders. Methods: We divided 129 participants into a high treatment resistance (HTR) group (current treatment with two SGAs, or clozapine, or classic neuroleptics for a failure of previous SGAs trials) and a low treatment resistance (LTR) group (current treatment with one atypical antipsychotic). We used Next-Generation Sequencing on DNA isolated from peripheral blood samples to analyze the polymorphisms. We performed logistic regression to search for predictors of HTR membership. Results: A diagnosis of schizophrenia significantly predicted the HTR membership compared to other diagnoses. Other predictors were the DRD3 rs6280 C|T (OR = 22.195) and T|T (OR = 18.47) vs. C|C, HTR2A rs7997012 A|G vs. A|A (OR = 6.859) and vs. G|G (OR = 2.879), and DRD2 rs1799732 I|I vs. D|I (OR = 12.079) genotypes. Conclusions: A diagnosis of schizophrenia and the DRD2 rs1799732, DRD3 rs6280, and HTR2A rs7997012 genotypes can predict high treatment resistance to SGAs.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/37509727; info:eu-repo/semantics/altIdentifier/wos/WOS:001034977200001; volume:11; issue:7; firstpage:1; lastpage:13; numberofpages:13; journal:BIOMEDICINES; https://hdl.handle.net/11573/1686429Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85175100983

الإتاحة: https://doi.org/10.3390/biomedicines11072088Test
https://hdl.handle.net/11573/1686429Test

المؤلفون: Botticelli, Andrea, Cerbelli, Bruna, Lionetto, Luana, Zizzari, Ilaria, Pisano, A, Roberto, Michela, ONESTI, Concetta Elisa, Di Pietro, Francesca, Napoletano, Chiara, Pizzuti, L, Vici, Patrizia, D'Amati, Giulia, Mazzuca, Federica, Simmaco, Maurizio, Nuti, Patrizia, Marchetti, Paolo

المصدر: The key role of kynurenine in antiPD-1 failure. Cancer Research., MDAmerican Association for Cancer Research. (2018).

مصطلحات موضوعية: Human health sciences :: Oncology, Sciences de la santé humaine :: Oncologie

العلاقة: AACR Annual Meeting 2018 (From 14 to 18 April 2018)

الوصول الحر: https://orbi.uliege.be/handle/2268/222231Test

المؤلفون: Del Casale, Antonio, Modesti, Martina Nicole, Gentile, Giovanna, Guariglia, Cecilia, Ferracuti, Stefano, Simmaco, Maurizio, Borro, Marina

المصدر: Current Issues in Molecular Biology; Jun2024, Vol. 46 Issue 6, p5322-5336, 15p

المؤلفون: Abdolrahimzadeh, Solmaz, Evangelisti, Melania, Gattazzo, Irene, Arpinelli, Marta, Di Nardo, Giovanni, Federico, Di Staso, Simmaco, Maurizio, Salerno, Gerardo, Parisi, Pasquale, Scuderi, Gianluca, Villa, Maria Pia

المصدر: Sleep & Breathing; May2024, Vol. 28 Issue 2, p639-646, 8p

مستخلص: Objectives: To investigate the vascular networks of the retina and choroid using optical coherence tomography angiography (OCTA) to identify early biomarkers of obstructive sleep apnea (OSA) severity and to evaluate correlations with blood levels of oxidative stress. Study design: Patients with OSA were diagnosed based on video-polysomnography (PSG) and blood samples were collected to evaluate oxidative stress markers: total antioxidant status (TAS), biological antioxidant potential (BAP) test, Diacron reactive oxygen metabolites (d-ROMs) test. The eyes of children with OSA were evaluated and compared with eyes of healthy age-matched children. OCTA imaging was carried out to evaluate the choroidal and retinal vascular network density indices. Results: A total of 31 children with OSA were recruited and compared with 10 healthy children. Choriocapillaris flow area decreased (p = 0.006) and superficial capillary plexus vessel density increased (p=0.01) with increasing severity of OSA. Children with OSA showed significant differences in TAS and d-ROMs test when compared to normal pediatric values (p<0.05). In calculating the correlations between PSG, oxidative stress, and OCTA variables, there was a negative correlation between choriocapillaris flow area and apnea hypopnea index (AHI) (p = 0.02, r² −0.5) and between choriocapillaris flow area and the d-ROMs test (p 0.03; r² 0.5). Conclusions: The severity of OSA was associated with the choroidal and retinal capillary vascular networks. The correlation of the choriocapillaris flow area with AHI and the d-ROMs test indicates the connection of the choroidal microvasculature with the number of obstructive apnea and hypopnea events and oxidative stress. [ABSTRACT FROM AUTHOR]

: Copyright of Sleep & Breathing is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Visco, Vincenzo, Lippi, Maria Enrichetta, Salerno, Gerardo, Licata, Maria Angela Vittoria A C, de Dominicis, Chiara, Antolino, Giusy, La Verde, Giacinto, Santino, Iolanda, Simmaco, Maurizio, Sciacchitano, Salvatore

المساهمون: Visco, Vincenzo, Lippi, Maria Enrichetta, Salerno, Gerardo, Licata, Maria Angela Vittoria A C, de Dominicis, Chiara, Antolino, Giusy, La Verde, Giacinto, Santino, Iolanda, Simmaco, Maurizio, Sciacchitano, Salvatore

مصطلحات موضوعية: CLL, CLL infection, antibodie, molecular diagnosi, virology

الوصف: We report here a case of a patient affected by B-cell chronic lymphocytic leukemia (CLL) that developed COVID-19 during the actual SARS-CoV-2 outbreak. The coexistence of CLL and COVID-19 raises many questions regarding the possible increased risk of developing COVID-19 among patients with CLL, the problems in managing therapies for both diseases and, above all, the difficulties in diagnosing COVID-19 in patients affected by CLL. In our patient, an 84-year-old man, the recognition of COVID-19 was delayed because of its atypical clinical presentation and technical problems related to the methods used for the diagnosis. Based on the symptoms and the radiological aspect of the lung, the occurrence of COVID-19 was suspected. Repeated tests on oro/nasopharyngeal swabs gave negative results, causing a delay in the diagnosis. Moreover, different methods used to identify the SARS-CoV-2 antibodies in serum gave conflicting results, and only two tests were able to identify SARS-CoV-2 Abs of the IgG type. During the clinical course of unrecognized COVID-19, our patient developed severe complications and did not receive any specific treatment for the two diseases. Recognition of COVID-19 in patients with CLL is a challenging task and the most accurate methods are necessary to overcome the diagnostic difficulties encountered.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/35323177; info:eu-repo/semantics/altIdentifier/wos/WOS:000775325900001; volume:14; issue:1; firstpage:31; lastpage:37; numberofpages:7; journal:HEMATOLOGY REPORTS; http://hdl.handle.net/11573/1627837Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85128776007

الإتاحة: https://doi.org/10.3390/hematolrep14010006Test
http://hdl.handle.net/11573/1627837Test

المؤلفون: Del Casale, Antonio, Pomes, Leda Marina, Bonanni, Luca, Fiaschè, Federica, Zocchi, Clarissa, Padovano, Alessio, De Luca, Ottavia, Angeletti, Gloria, Brugnoli, Roberto, Girardi, Paolo, Preissner, Robert, Borro, Marina, Gentile, Giovanna, Pompili, Maurizio, Simmaco, Maurizio

المساهمون: Del Casale, Antonio, Pomes, Leda Marina, Bonanni, Luca, Fiaschè, Federica, Zocchi, Clarissa, Padovano, Alessio, De Luca, Ottavia, Angeletti, Gloria, Brugnoli, Roberto, Girardi, Paolo, Preissner, Robert, Borro, Marina, Gentile, Giovanna, Pompili, Maurizio, Simmaco, Maurizio

مصطلحات موضوعية: pharmacogenomic, treatment-resistant depression, mood disorder, major depressive disorder, bipolar disorder

الوصف: Treatment-resistant depression (TRD) reduces affected patients’ quality of life and leads to important social health care costs. Pharmacogenomics-guided treatment (PGT) may be effective in the cure of TRD. The main aim of this study was to evaluate the clinical changes after PGT in patients with TRD (two or more recent failed psychopharmacological trials) affected by bipolar disorder (BD) or major depressive disorder (MDD) compared to a control group with treatment as usual (TAU). We based the PGT on assessing different gene polymorphisms involved in the pharmacodynamics and pharmacokinetics of drugs. We analyzed, with a repeated-measure ANOVA, the changes between the baseline and a 6 month follow-up of the efficacy index assessed through the Clinical Global Impression (CGI) scale, and depressive symptoms through the Hamilton Depression Rating Scale (HDRS). The PGT sample included 53 patients (26 BD and 27 MDD), and the TAU group included 52 patients (31 BD and 21 MDD).We found a significant within-subject effect of treatment time on symptoms and efficacy index for the whole sample, with significant improvements in the efficacy index (F = 8.544; partial 2 = 0.077, p < 0.004) and clinical global impression of severity of illness (F = 6.818; partial 2 = 0.062, p < 0.01) in the PGT vs. the TAU group. We also found a significantly better follow-up response (2 = 5.479; p = 0.019) and remission (2 = 10.351; p = 0.001) rates in the PGT vs. the TAU group. PGT may be an important option for the long-term treatment of patients with TRD affected by mood disorders, providing information that can better define drug treatment strategies and increase therapeutic improvement.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/35207804; info:eu-repo/semantics/altIdentifier/wos/WOS:000763665500001; volume:12; issue:2; firstpage:1; lastpage:13; numberofpages:13; journal:JOURNAL OF PERSONALIZED MEDICINE; http://hdl.handle.net/11573/1612785Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85125353902

الإتاحة: https://doi.org/10.3390/jpm12020316Test
http://hdl.handle.net/11573/1612785Test

المؤلفون: De Vitis, Claudia, Capalbo, Carlo, Torsello, Alessandra, Napoli, Christian, Salvati, Valentina, Loffredo, Chiara, Blandino, Giovanni, Piaggio, Giulia, Auciello, Francesca Romana, Pelliccia, Flaminia, Salerno, Gerardo, Simmaco, Maurizio, Di Magno, Laura, Canettieri, Gianluca, Coluzzi, Flaminia, Mancini, Rita, Rocco, Monica, Sciacchitano, Salvatore

المساهمون: De Vitis, Claudia, Capalbo, Carlo, Torsello, Alessandra, Napoli, Christian, Salvati, Valentina, Loffredo, Chiara, Blandino, Giovanni, Piaggio, Giulia, Auciello, Francesca Romana, Pelliccia, Flaminia, Salerno, Gerardo, Simmaco, Maurizio, Di Magno, Laura, Canettieri, Gianluca, Coluzzi, Flaminia, Mancini, Rita, Rocco, Monica, Sciacchitano, Salvatore

مصطلحات موضوعية: COVID-19, bioelectrical impedance analysis (BIA), biological antioxidant potential (BAP), extracellular flux analyzer, mitochondrial respiration, nonthyroidal illness syndrome (NTIS), oxidative stre, reactive oxygen species (ROS), thyroid hormones (THs)

الوصف: Background: Thyroid hormones (TH)s are master regulators of mitochondrial activity and biogenesis. Nonthyroidal illness syndrome (NTIS) is generally considered an adaptative response to reduced energy that is secondary to critical illness, including COVID-19. COVID-19 has been associated with profound changes in the cell energy metabolism, especially in the cells of the immune system, with a central role played by the mitochondria, considered the power units of every cell. Infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) affects and alters mitochondrial functions, both to influence its intracellular survival and to evade host immunity. Aim of the study: This study was undertaken to analyze the oxidative balance and mitochondrial respiration in COVID-19 patients with and without NTIS to elucidate the role that thyroid hormones (TH)s play in this context. Methods: In our cohort of 54 COVID-19 patients, admitted to our University Hospital during the COVID-19 pandemic, we evaluated the generation of reactive oxygen species (ROS) by measuring the serum levels of derivatives of reactive oxygen metabolites (dROMs), and we analyzed the antioxidant capacity by measuring the serum biological antioxidant potential (BAP). We then analyzed the mitochondrial respiration in peripheral blood mononuclear cells (PBMC)s of 28 of our COVID-19 patients, using the seahorse instrument (Agilent). Results were correlated with the serum levels of THs and, in particular, of FT3. In addition, the role of T3 on bioelectrical impedance analysis (BIA) and mitochondrial respiration parameters was directly evaluated in two COVID-19 patients with NTIS, in which treatment with synthetic liothyronine (LT3) was given both in vivo and in vitro. Results: In our COVID-19 patients with NTIS, the dROMs values were significantly lower and the BAP values were significantly higher. Consequently, the oxidative stress index (OSi), measured as BAP/dROMs ratio was reduced compared to that observed in COVID-19 patients without NTIS, ...

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/36290721; info:eu-repo/semantics/altIdentifier/wos/WOS:000872096700001; volume:11; issue:10; firstpage:1; lastpage:22; numberofpages:22; journal:ANTIOXIDANTS; https://hdl.handle.net/11573/1658043Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85140491097

الإتاحة: https://doi.org/10.3390/antiox11101998Test
https://hdl.handle.net/11573/1658043Test

المؤلفون: Salamone, Sandro, Spirito, Sara, Simmaco, Maurizio, Unger, Marius, Preissner, Saskia, Gohlke, Björn-Oliver, Eckert, Andreas, Preissner, Robert

المساهمون: Salamone, Sandro, Spirito, Sara, Simmaco, Maurizio, Unger, Mariu, Preissner, Saskia, Gohlke, Björn-Oliver, Eckert, Andrea, Preissner, Robert

مصطلحات موضوعية: cyp, ddgi, personalized medicine, precision medicine, single nucleotide polymorphism, transporter

الوصف: Purpose: Pharmacogenetic counselling is a complex task and requires the efforts of an interdisciplinary team, which cannot be implemented in most cases. Therefore, simple rules could help to minimize the risk of medications incompatible with each other or with frequent genetic variants. Patients and Methods: One hundred and eighty-four multi-morbid Caucasian patients suffering from side effects or inefficient therapy were enrolled and genotyped. Their medication was analyzed by a team of specialists using Drug-PIN (R) (medication support system) and individual recommendations for 34 drug classes were generated. Results: In each of the critical drug classes, 50% of the drugs cannot be recommended to be prescribed in typical drug cocktails. PPIs and SSRI/SNRIs represent the most critical drug classes without showing a single favorable drug. Among the well-tolerated drugs (not recommended for less than 5% of the patients) are metamizole, celecoxib, olmesartan and famotidine. For each drug class, a ranking of active ingredients according to their suitability is presented. Conclusion: Genotyping and its profound analysis are not available in many settings today. The consideration of frequent alterations of metabolic elimination routes and drug-drug-gene interactions by using simple rankings can help to avoid many incompatibilities, side effects and inefficient therapies.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/36004008; info:eu-repo/semantics/altIdentifier/wos/WOS:000848564000001; volume:15; firstpage:765; lastpage:773; numberofpages:9; journal:PHARMACOGENOMICS AND PERSONALIZED MEDICINE; https://hdl.handle.net/11573/1684259Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85136318087

الإتاحة: https://doi.org/10.2147/PGPM.S368606Test
https://hdl.handle.net/11573/1684259Test