المؤلفون: Grettka, Krzysztof, Idzik, Katarzyna, Lewandowska, Katarzyna, Świętek, Ksena, Palini, Simone, Silvestris, Franco

المصدر: Biomedicines; Jun2024, Vol. 12 Issue 6, p1139, 9p

مصطلحات موضوعية: STEM cells, INFERTILITY, PREMATURE ovarian failure, FERTILIZATION in vitro, FERTILITY preservation

مستخلص: Today, women's infertility is considered a social disease in females, occurring not only as an effect of POF (premature ovarian failure) but also as CTRI (cancer treatment-related infertility) in oncologic patients. Several procedures for FP (fertility preservation) are currently adopted to prevent this condition, mostly based on utilization of retrieved eggs from the patients with subsequent IVF (in vitro fertilization) or cryopreservation. However, great interest has recently been devoted to OSCs (ovarian stem cells), whose isolation from female ovaries, followed by their in vitro culture, led to their maturation to OLCs (oocyte-like cells), namely, neo-oocytes comparable to viable eggs suitable for IVF. Translation of these data to FP clinical application creates new hope in the treatment of infertility. Thus, in line with the significant progress in using stem cells in the regenerative medicine field, neo-oogenesis via OSCs, which is currently unapplicable in fertility preservation procedures, will provide novel possibilities for young and adult females in motherhood programs in the future. [ABSTRACT FROM AUTHOR]

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المؤلفون: D'oronzo, Stella, Lovero, Domenica, Palmirotta, Raffaele, Cafforio, Paola, Brown, Janet, Wood, Steven, Porta, Camillo, Silvestris, Franco

المصدر: Bone Reports ; volume 14, page 100840 ; ISSN 2352-1872

مصطلحات موضوعية: Orthopedics and Sports Medicine, Endocrinology, Diabetes and Metabolism

الإتاحة: https://doi.org/10.1016/j.bonr.2021.100840Test
https://api.elsevier.com/content/article/PII:S2352187221000954?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2352187221000954?httpAccept=text/plainTest

المؤلفون: Gentile, Marica, Centonza, Antonella, Lovero, Domenica, Palmirotta, Raffaele, Porta, Camillo, Silvestris, Franco, D'Oronzo, Stella

المساهمون: Regione Puglia

المصدر: Journal of Bone Oncology ; volume 26, page 100337 ; ISSN 2212-1374

مصطلحات موضوعية: Oncology

الإتاحة: https://doi.org/10.1016/j.jbo.2020.100337Test
https://api.elsevier.com/content/article/PII:S2212137420300920?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2212137420300920?httpAccept=text/plainTest

المؤلفون: De Pergola, Giovanni, Martino, Tommaso, Zupo, Roberta, Caccavo, Domenico, Pecorella, Claudio, Paradiso, Silvia, Silvestris, Franco, Triggiani, Vincenzo

المساهمون: De Pergola, Giovanni, Martino, Tommaso, Zupo, Roberta, Caccavo, Domenico, Pecorella, Claudio, Paradiso, Silvia, Silvestris, Franco, Triggiani, Vincenzo

مصطلحات موضوعية: Fat ma, Obesity, Vitamin D

الوصف: Obesity is associated with lower serum vitamin D (25(OH)D) levels through several mechanisms. The aim of the study was to examine the possibility of a negative association between fat mass and 25(OH)D levels in a cohort of otherwise healthy overweight and obese subjects, independently of age, sex, blood pressure levels and anthropometric and metabolic parameters.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/30666920; info:eu-repo/semantics/altIdentifier/wos/WOS:000484371400013; volume:19; firstpage:838; lastpage:844; numberofpages:7; journal:ENDOCRINE, METABOLIC & IMMUNE DISORDERS DRUG TARGETS; http://hdl.handle.net/11586/227832Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85070607974

الإتاحة: https://doi.org/10.2174/1871530319666190122094039Test
http://hdl.handle.net/11586/227832Test

المؤلفون: Tucci Marco, Passarelli Anna, Todisco Annalisa, Mannavola Francesco, Stucci Luigia Stefania, D'Oronzo Stella, Rossini Michele, Taurisano Marco, Gesualdo Loreto, Silvestris Franco

المساهمون: Tucci, Marco, Passarelli, Anna, Todisco, Annalisa, Mannavola, Francesco, Stucci, LUIGIA STEFANIA, D'Oronzo, Stella, Rossini, Michele, Taurisano, Marco, Gesualdo, Loreto, Silvestris, Franco

مصطلحات موضوعية: acute interstitial nephriti, immunotherapy, melanoma, nephrotoxicity

الوصف: In this article, the authors’ first names and surnames were incorrectly listed in the wrong order. The correct author list is: Marco Tucci, Anna Passarelli, Annalisa Todisco, Francesco Mannavola, Luigia Stefania Stucci, Stella D’Oronzo, Michele Rossini, Marco Taurisano, Loreto Gesualdo and Franco Silvestris.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/31666813; info:eu-repo/semantics/altIdentifier/wos/WOS:000490985500001; volume:11; issue:Article Number: 1758835919885202; firstpage:1; lastpage:1; numberofpages:1; journal:THERAPEUTIC ADVANCES IN MEDICAL ONCOLOGY; http://hdl.handle.net/11586/312219Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85073500675

الإتاحة: https://doi.org/10.1177/1758835919885202Test
http://hdl.handle.net/11586/312219Test

المؤلفون: Tucci, Marco, D’Oronzo, Stella, Mannavola, Francesco, Felici, Claudia, Lovero, Domenica, Cafforio, Paola, Palmirotta, Raffaele, Silvestris, Franco

المساهمون: Apulia Region Jonico-Salentino Project, Apulia Region ‘Oncogenomic’ project, Apulia Region ‘Precision Medicine’ project, Associazione Italiana per la Ricerca sul Cancro

المصدر: Therapeutic Advances in Medical Oncology ; volume 12, page 175883592090541 ; ISSN 1758-8359 1758-8359

مصطلحات موضوعية: Oncology

الوصف: Background: Circulating tumor cells (CTCs) have recently emerged as a new dynamic soluble marker for several malignancies including cutaneous melanoma (CM) and are suitable for prognostic evaluations and treatment monitoring. However, to date many limitations still hamper the wide-scale application of CTCs in CM setting, including the lack of standardized methods as well as both low levels and heterogeneity of these cells. Methods: We developed a protocol for CTC detection in CM based on immune-magnetic sorting to deplete CD45-, CD31- or CD34-positive cells, followed by dielectrophoretic DEPArray separation according to cell morphology and immunophenotype. To this end, we explored the expression of melanoma stem cell antigens (CD271, ABCB5, and RANK) and the epithelial-to-mesenchymal transition markers (N-Cad, -CD44, and -MCAM/CD146) on CTCs from 17 stage IV CM patients, and investigated their BRAF mutational status by droplet digital PCR. Results: The number of CTCs isolated from CM patients ranged from 2 to 91 cells (38 ± 6.4) with respect to healthy donors ( p < 0.0002). To confirm the melanoma origin of isolated cells, we observed an 80% agreement between their BRAF V600 mutational status and matched primary tumors. The characterization of the immune phenotype of isolated cells revealed high interindividual and intraindividual heterogeneity that was found to correlate with the clinical outcome. Conclusions: The dual-step protocol of immune-magnetic sorting and subsequent dielectrophoretic DEPArray separation, turned out to be a suitable method to isolate viable CTCs from stage IV melanoma patients and enabled quantitative and qualitative analyses on these cells, which may deserve prospective evaluation for potential use in the clinical practice.

الإتاحة: https://doi.org/10.1177/1758835920905415Test

المؤلفون: Cives, Mauro, Pellè, Eleonora, Rinzivillo, Maria, Prosperi, Daniela, Tucci, Marco, Silvestris, Franco, Panzuto, Francesco

المصدر: Neuroendocrinology ; volume 111, issue 3, page 207-216 ; ISSN 0028-3835 1423-0194

الوصف: Skeletal colonization is often regarded as a rare event in patients with neuroendocrine tumors (NETs) although both national registries and retrospective series report an incidence of bone metastases as high as 20% in subjects with advanced disease. While the biological mechanisms leading to bone metastatic colonization in NETs have been poorly investigated so far, key steps of osteotropic mechanisms, including the epithelial-to-mesenchymal transition, preparation of the premetastatic niche, migration of circulating tumor cells towards the bone marrow as well as the resulting alterations of the skeletal metabolism, are likely to operate also during the development of NET bone metastases. The skeleton involvement by NETs has a detrimental impact on both quality of life and patients’ prognosis, leading to pain in the majority of symptomatic subjects. While it is currently unclear whether or not the earlier recognition of bone involvement by PET/CT imaging techniques employing 68 Ga-DOTA-conjugated peptides might improve outcomes through the exploitation of timely treatments, the management of bone-colonizing NETs is today based only on clinical experience from other osteotropic tumors. Here, we summarize the fundamental molecular mechanisms driving bone colonization and revisit both established and novel treatments for patients with bone metastatic NETs.

الإتاحة: https://doi.org/10.1159/000508633Test
https://www.karger.com/Article/Pdf/508633Test

المؤلفون: Mannavola, Francesco, Mandala, Mario, Todisco, Annalisa, Sileni, Vanna Chiarion, Palla, Marco, Minisini, Alessandro Marco, Pala, Laura, Morgese, Francesca, Di Guardo, Lorenza, Stucci, Luigia Stefania, Guida, Michele, Indini, Alice, Quaglino, Pietro, Ferraresi, Virginia, Marconcini, Riccardo, Tronconi, Maria Chiara, Rossi, Ernesto, Nigro, Olga, Occelli, Marcella, Cortellini, Alessio, Quadrini, Silvia, Palmieri, Giuseppe, Pigozzo, Jacopo, Ascierto, Paolo Antonio, Vitale, Maria Grazia, Strippoli, Sabino, Ferrucci, Pier Francesco, Berardi, Rossana, Randon, Giovanni, Cardone, Pietro, Schinzari, Giovanni, Silvestris, Franco, Tucci, Marco

المصدر: Frontiers in Oncology ; volume 10 ; ISSN 2234-943X

الإتاحة: https://doi.org/10.3389/fonc.2020.01652Test

المؤلفون: Internò, Valeria, Tucci, Marco, Pezzicoli, Gaetano, Silvestris, Franco, Porta, Camillo, Mannavola, Francesco

المساهمون: Regione Puglia

المصدر: Frontiers in Oncology ; volume 10 ; ISSN 2234-943X

مصطلحات موضوعية: Cancer Research, Oncology

الإتاحة: https://doi.org/10.3389/fonc.2020.581130Test

المؤلفون: Tucci, Marco, Mannavola, Francesco, Passarelli, Anna, Stucci, Luigia Stefania, Cives, Mauro, Silvestris, Franco

المساهمون: Tucci, Marco, Mannavola, Francesco, Passarelli, Anna, Stucci, Luigia Stefania, Cives, Mauro, Silvestris, Franco

مصطلحات موضوعية: Exosome, Immune system, Melanoma, Oncology

الوصف: Exosomes (Exo) are small vesicles produced by melanoma cells and the accessory cells of the tumor microenvironment. They emerge via both classical and direct pathways and actively participate in tumor colonisation of distant tissues. The proteins, nucleic acids, cytokines and growth factors engulfed by Exo are transferred to recipient cells, where they drive numerous functions required for the tumor escape from immune system control and tumor progression. By positively or negatively modulating immune cell properties, Exo provoke immune suppression and, in turn, defective dendritic cell (DC) functions. Together, these effects limit the cytotoxicity of T-cells and expand both T-regulatory and myeloid-derived suppressor populations. They also hinder perforin and granzyme production by natural killer cells. Finally, Exo also control the organotropism of melanoma cells. The distinct phenotypic properties of Exo can be exploited both for diagnostic purposes and in the early identification of melanoma patients likely to respond to immunotherapy. The potential therapeutic application of Exo derived from DCs has been demonstrated in vaccination trials, which showed an increase in anti-melanoma activity with respect to circulating tumor cells. However, additional studies are required before Exo can be effectively used in diagnostic and therapeutic applications in melanoma.

العلاقة: volume:9; issue:29; firstpage:20826; lastpage:20837; numberofpages:12; journal:ONCOTARGET; http://hdl.handle.net/11586/219306Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85045508035; http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=download&pathTest[]=24846&path[]=77911

الإتاحة: https://doi.org/10.18632/oncotarget.24846Test
http://hdl.handle.net/11586/219306Test
http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=download&pathTest[]=24846&path[]=77911