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71
المؤلفون: Al Sinawi S, Al Dhahli B, Al Issaei H, Al Wahaibi L, Alwahaibi N
مصطلحات موضوعية: medicine.medical_specialty, Reticular fiber, Traditional medicine, Tissue fixative, digestive, oral, and skin physiology, H&E stain, food and beverages, Biology, Staining, Jones methenamine silver stain, Rat liver, medicine, Histopathology, Fixative
الوصف: In the routine laboratory, 10% neutral buffered formalin (NBF) is the fixative of choice. However, formalin is a human carcinogen. To the best of our knowledge, neutral honey, not natural or artificial honey, has not been tested to fix histological tissues. This study aimed to examine the efficiency of neutral buffered honey and other types of honey fixatives to fix histological tissues. The most two natural common Omani honey were used as fixatives, namely Sumar and date. We tested samples of rat liver, kidney, and stomach. Nine types of fixatives were used. All tissues were treated equally. The evaluation was performed blindly by three senior biomedical scientists who work in a histopathology laboratory. Hematoxylin and eosin showed adequate staining in all groups when compared to 10% NBF. The intensity and specificity of Jones Methenamine silver stain in 10% Sumer and Date honey and 10% alcoholic Sumer honey showed similar findings of 10% NBF. The specificity and intensity of all groups for Periodic acid–Schiff were comparable with 10% neutral buffered formalin accepts for 10% Sumer honey and 10% Alcoholic Date honey. However, all honey groups showed weak staining for the reticulin fibers using Gordon and Sweets method. Vimentin showed comparable findings with 10% NBF as there were no significant differences. The findings of this study are promising. Further in depth research on honey as a possible safe substitute fixative for formalin should be conducted.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::8104e2a71f4b5c766b458010503dc810Test
https://doi.org/10.1101/2021.04.27.437988Test -
72
المصدر: Journal of the American Society of Cytopathology. 10(6)
مصطلحات موضوعية: Clinical team, Retrospective review, medicine.medical_specialty, Staining and Labeling, business.industry, Cytodiagnosis, Cytological Techniques, Grocott's methenamine silver stain, Stain, Pathology and Forensic Medicine, Cost savings, Cytology, Clinical information, Medicine, Ascitic Fluid, Humans, business, Intensive care medicine, Bronchoalveolar Lavage Fluid, Retrospective Studies
الوصف: Introduction Bronchoalveolar lavage (BAL) and body cavity fluids (pleural and peritoneal) have a useful role in the detection of infectious diseases, especially when combined with ancillary stains (Grocott methenamine silver, acid-fast bacilli, Fite, and cytomegalovirus). However, empirical ordering of stains and duplicate testing by concurrent microbiology cultures leads to unnecessary wastage of resources. The aim of our study was to optimize the use of resources. Materials and methods We performed a retrospective review of all BALs and body cavity fluids from 2016 to 2018 to determine the baseline stain order rate, which was then used in conjunction with clinical information to create a reasonable target for 2019. The methods implemented included communication with the clinical team to modify current ordering practice, monitoring stain orders prospectively through 2019, and updating the cytology requisition form to limit upfront ordering of ancillary stains. Results From 2016 to 2018, of the 1270 specimens reviewed, 323 (55%) were BALs and 108 (16%) were body cavity fluids that had had ≥2 stains preordered. This was reduced by the end of 2019 to 10% of BALs and 3.4% of body cavity fluids, leading to $25,935.24 in costs savings (including 275 hours of technician time and 39.3 hours of pathologist time). Conclusions We found that ancillary microorganism stains have limited utility in cytology specimens and should only be ordered when indicated after a review of the initial smears. In today’s era of cost savings, upfront ordering of ancillary stains can lead to significant wastage of resources that can be prevented by improving workflow.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ff810812a9495e82954f8e57072ae4bTest
https://pubmed.ncbi.nlm.nih.gov/34507918Test -
73
المؤلفون: Raudu Rajendra Kumar Reddy, P Das, Pramoda Kumar Sahoo, Jyotirmaya Mohanty, Amol Suryawanshi, Mohan R Badhe
المصدر: Parasite Immunology. 43
مصطلحات موضوعية: 0301 basic medicine, Carps, Two-dimensional gel electrophoresis, biology, Protein subunit, 030231 tropical medicine, Immunology, Cyprinidae, Heterologous, biology.organism_classification, Immunoproteomics, Labeo, Silver stain, Fish Diseases, 03 medical and health sciences, Argulus siamensis, 030104 developmental biology, 0302 clinical medicine, Arguloida, Antigen, Biochemistry, Tandem Mass Spectrometry, Animals, Parasitology
الوصف: Aim An immunoproteomic approach was followed to identify immunoreactive antigens of fish ectoparasite, Argulus siamensis with rohu (Labeo rohita) immune sera for screening of potential vaccine candidates. Materials and results The whole adult Argulus antigen was run in 2D electrophoresis with IEF in 7 cm IPG strips of pH 4-7 and SDS-PAGE with 12% acrylamide concentration. Two parallel gels were run; one was stained with silver stain, and the other was Western blotted to nitrocellulose paper (NCP) and reacted with rohu anti-A siamensis sera. Fourteen protein spots corresponding to the spots developed in NCP were picked from the silver-stained gel and subjected to mass spectrometry in MALDI-TOF/TOF. The MS/MS spectra were analysed in MASCOT software with taxonomy 'other metazoa' and the proteins identified based on similarity with the proteins from heterologous species. The gene ontology analysis revealed a majority of proteins being involved in binding activity in 'molecular function' and belonging to metabolic processes in 'biologic process' categories. The possibility of these proteins as vaccine candidates against A siamensis is discussed in the paper. Conclusion Three of the identified proteins namely, bromodomain-containing protein, anaphase-promoting complex subunit 5 and elongation factor-2 could possibly serve as vaccine candidates against argulosis in carps.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f18c18c53d0dab749488737948f887f3Test
https://doi.org/10.1111/pim.12837Test -
74
المؤلفون: Maksim A Yakovlev, Dilworth Y. Parkinson, Keith C. Cheng, Yifu Ding, Spencer R Katz, Daniel J. Vanselow, Khai C. Ang, Victor A. Canfield, Yuxin Wang, Alex Y. Lin
المصدر: eLife
eLife, Vol 10 (2021)مصطلحات موضوعية: Silver Staining, QH301-705.5, Science, ved/biology.organism_classification_rank.species, Mutant, Human skin, Context (language use), micro-CT, General Biochemistry, Genetics and Molecular Biology, Imaging, Silver stain, Melanin, developmental biology, Imaging, Three-Dimensional, 3D imaging, cell biology, Animals, pigmentation, Biology (General), Model organism, whole-organism phenomics, Zebrafish, Melanins, General Immunology and Microbiology, biology, ved/biology, Chemistry, General Neuroscience, General Medicine, X-Ray Microtomography, Cell Biology, Zebrafish Proteins, biology.organism_classification, zebrafish, Chromatophore, Phenotype, Cell biology, melanin, Biochemistry, Three-Dimensional, Medicine, Biomedical Imaging, Biochemistry and Cell Biology, Research Advance, Whole Organism, Developmental Biology
الوصف: We previously described X-ray histotomography, a high-resolution, non-destructive form of X-ray microtomography (micro-CT) imaging customized for three-dimensional (3D), digital histology, allowing quantitative, volumetric tissue and organismal phenotyping (Ding et al., 2019). Here, we have combined micro-CT with a novel application of ionic silver staining to characterize melanin distribution in whole zebrafish larvae. The resulting images enabled whole-body, computational analyses of regional melanin content and morphology. Normalized micro-CT reconstructions of silver-stained fish consistently reproduced pigment patterns seen by light microscopy, and further allowed direct quantitative comparisons of melanin content across wild-type and mutant samples, including subtle phenotypes not previously noticed. Silver staining of melanin for micro-CT provides proof-of-principle for whole-body, 3D computational phenomic analysis of a specific cell type at cellular resolution, with potential applications in other model organisms and melanocytic neoplasms. Advances such as this in whole-organism, high-resolution phenotyping provide superior context for studying the phenotypic effects of genetic, disease, and environmental variables.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a530c7306dc5b071f862f7ab690c30fdTest
https://pubmed.ncbi.nlm.nih.gov/34528510Test -
75
المؤلفون: Aydogan Ozcan, Yijie Zhang, Jingxi Li, Kevin de Haan, Yair Rivenson
المصدر: Label-free Biomedical Imaging and Sensing (LBIS) 2021.
مصطلحات موضوعية: Silver stain, Autofluorescence, business.industry, Trichrome, Computer science, Deep learning, Histology, Artificial intelligence, business, Stain, Histochemical staining, Biomedical engineering, Staining
الوصف: We virtually generate multiple histological stains through a single deep-neural-network, using at its input autofluorescence images of the unlabeled tissue alongside a user-defined digital-staining-matrix. By feeding this digital-staining-matrix to the network, the user indicates which stain to apply on each pixel or region-of-interest, enabling virtual blending of multiple stains according to a desired micro-structure map. We demonstrated this technique by applying combinations of different stains (H&E, Masson’s Trichrome and Jones silver stain) on blindly-tested, unlabeled tissue sections. This technology avoids the histochemical staining process and enables newly-generated stains and stain-combinations to be used for inspection of label-free tissue microstructure.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::f926d7f59b3235f13aad3a595aadc98aTest
https://doi.org/10.1117/12.2579426Test -
76
المؤلفون: Wes Baumgartner, Tzushan S. Yang, Orion S. Rivers
المصدر: Journal of comparative pathology. 187
مصطلحات موضوعية: Lung Diseases, Pathology, medicine.medical_specialty, Hemosiderosis, General Veterinary, Staining and Labeling, business.industry, ALIZARIN RED, Hemorrhage, Airway smooth muscle, Cat Diseases, Pathology and Forensic Medicine, Silver stain, Fatal Outcome, Respiratory failure, Cats, Medicine, Gandy–Gamna nodules, Animals, Female, Pulmonary vasculature, business, Animal species, Von Kossa stain, Lung
الوصف: Summary Mineralizing pulmonary elastosis (MPE) is a rare and unique phenomenon that has been reported in humans, typically secondary to recurrent pulmonary haemorrhage. MPE has a complex histopathological appearance, often containing iron-calcium deposits that can be mistaken as fungal organisms or other inorganic material. This report documents the first case of MPE in an animal species. A 10-year-old female domestic cat with respiratory failure was submitted for necropsy. The lungs were consolidated with severe pulmonary haemosiderosis, and widely disseminated granulomas surrounded large aggregates of hyphae-like structures. The pulmonary vasculature and airway smooth muscle were partially mineralized and fragmented. Histochemical stains revealed that the fungus-like material stained strongly with Prussian blue and alizarin red but only sparingly with von Kossa and negative with Gomori's methenamine silver stain. These findings are similar to those of MPE in humans. As most veterinary pathologists may not be familiar with MPE, it is important to avoid possible misinterpretation by recognizing its distinct features and the ancillary testing that may be required.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d5dd723fdab5a71183da8694b635bdcTest
https://pubmed.ncbi.nlm.nih.gov/34503649Test -
77دورية أكاديمية
المؤلفون: Shoiab, M., Irshadullah, M.1
المصدر: Journal of Veterinary Parasitology 23(2):147-149. 2009
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78دورية أكاديمية
المؤلفون: Xu, Feifan
المصدر: Senior Honors Theses
مصطلحات موضوعية: recombinant protein purification, antibody, co-immunoprecipitation, western blot, folate, gene expression, silver stain, Biochemistry, Cell Biology, Molecular Biology, Molecular, Genetic, and Biochemical Nutrition, Molecular Genetics
الوصف: A previous study that introduced dietary folate to mice in the form of folic acid to determine if gene activity would be altered based on this biological molecule demonstrated that mice without folic acid had cognition deficits, and this phenomenon was correlated with altered gene expression in their brains. The included bioinformatic analysis revealed two main transcription factors that bind to proteins in the nucleus, and one is known as the Zinc Finger Protein 410 (Zfp410). Due to the lack of literature explaining the function of this transcription factor, this project is intended to analyze Zfp410 in detail from scratch. Zfp410 cDNA sequence was purchased along with a plasmid vector, transferred to E. coli, and used to purify the recombinant protein. The purified protein was then injected into a rabbit for antibody production, and the collected Zfp410 antibody was characterized using several molecular techniques. Within high efficiency of targeting its antigen, Zfp410 antibody was used to identify any interacting proteins of Zfp410 by co-immunoprecipitation along with silver stain and western blot. The results demonstrated several Zfp410 interacting partners which suggested transcription factor Zfp410’s role in folate-dependent genes’ regulations.
وصف الملف: application/pdf
العلاقة: https://digitalcommons.liberty.edu/honors/1075Test; https://digitalcommons.liberty.edu/cgi/viewcontent.cgi?article=2127&context=honorsTest
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79
المؤلفون: Bhaskar Jyoti Sarmah, Naushad Shah, Brajendra Lahkar, Kuddush Ahmed, Ronica Baruah, Tauhidul Alam Choudhury
المصدر: Medical Mycology Case Reports
Medical Mycology Case Reports, Vol 24, Iss, Pp 78-81 (2019)مصطلحات موضوعية: 0301 basic medicine, medicine.medical_specialty, Mass lesion, MRI, Magnetic resonance imaging, Itraconazole, 030231 tropical medicine, 030106 microbiology, Oropharynx, Case Report, Malignancy, Microbiology, Histoplasmosis, PAS, periodic acid-Schiff stain, Lesion, 03 medical and health sciences, 0302 clinical medicine, Swallowing, Tongue, Amphotericin B, Biopsy, medicine, GMS, Grocott methanamine silver stain, HIV, human Immune deficiency virus, lcsh:QH301-705.5, lcsh:R5-920, medicine.diagnostic_test, business.industry, CT, Computerized Tomography, medicine.disease, Dermatology, Infectious Diseases, medicine.anatomical_structure, lcsh:Biology (General), Disseminated histoplasmosis, medicine.symptom, business, lcsh:Medicine (General), DH, Disseminated Histoplasmosis, medicine.drug
الوصف: A 62 year old man from Silchar in North East India presented with complaints of painful swallowing, hoarseness, fever, anorexia and weight loss. Oropharyngeal examination revealed reddish ulcero-nodular lesion involving left tonsillar area and base of tongue which was clinically suspicious of malignancy. Radiological examination revealed involvement of bilateral adrenals by a mass. The biopsy of the oropharyngeal lesion showed many fungal spores morphologically favoring Histoplasmosis. Treatment with Amphotericin B followed by Itraconazole resulted recovery of lesions. Keywords: Disseminated histoplasmosis, Oropharynx, Mass lesion
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33ce3c9b5c38b30ee6894edcf135d848Test
http://europepmc.org/articles/PMC6503129Test -
80
المؤلفون: M M Kamal, Hirakani Mankar, Kanica Jha
المصدر: IP Archives of Cytology and Histopathology Research. 4:31-35
مصطلحات موضوعية: Silver stain, Flora, Pap smears, medicine.medical_specialty, business.industry, Cytopathology, Medicine, Effective treatment, Papanicolaou stain, business, Microwave assisted, Dermatology, Stain
الوصف: Introduction: Pap smear has been utilized since a long time in detecting cervical pre-cancers and cancers. Their potential in diagnosing pathogenic organisms however is underestimated. Reporting organisms morphologically consistent with Candida species, in cervicovaginal smears, is easier, but coexisting coccobacillary flora is often missed in the bargain. Such co-existing infections make the treatment difficult for clinicians, prolonging the distressing symptoms of patients. We evaluated modified silver staining technique using a microwave - JONES MARRES STAIN (JM) and tried comparing the results with conventional Pap smears to find any additional flora. Aim: To identify co-existing organisms in addition to Candida in a Pap smear stained with Jones Marres stain. Materials and methods: This retrospective prevalence study that included 82 consecutive conventional Pap smears routinely stained with Pap stain was then subjected to silver stain in a microwave. Inclusion criteria: Pap smears reported as showing Candida group of organisms. Results: In the 82 smears evaluated, JM stain helped in easier picking up of additional coccobacillary flora in 37(45%) of cases in comparison to 4(4.87%).These coexisting infections were missed in conventional Pap smear reporting. Conclusion: Modified JM stain helped in diagnosing additional flora thus reducing the chances of missing out co-infections and hence is useful for providing effective treatment Keywords: Microwave Silver stain, Co-infection, Pap smear, Coccobacillary flora.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::3565eca0018557fcfa7479d9a87fa2c4Test
https://doi.org/10.18231/2456-9267.2019.0005Test
