المؤلفون: Zhang, Weiqing, Willows, Robert D, Deng, Rui, Li, Zheng, Li, Mengqi, Wang, Yan, Guo, Yunling, Shi, Weida, Fan, Qiuling, Martin, Shelley S, Rockwell, Nathan C, Lagarias, J Clark, Duanmu, Deqiang

المصدر: Proceedings of the National Academy of Sciences of the United States of America. 118(20)

مصطلحات موضوعية: Plant Biology, Biological Sciences, Bile Pigments, Chlamydomonas reinhardtii, Chlorophyll, Cyanobacteria, Heme Oxygenase (Decyclizing), Intracellular Signaling Peptides and Proteins, Lyases, Protoporphyrins, heme oxygenase, bilin reductase, reactive oxygen species, photosynthesis, phycocyanobilin

الوصف: Biosyntheses of chlorophyll and heme in oxygenic phototrophs share a common trunk pathway that diverges with insertion of magnesium or iron into the last common intermediate, protoporphyrin IX. Since both tetrapyrroles are pro-oxidants, it is essential that their metabolism is tightly regulated. Here, we establish that heme-derived linear tetrapyrroles (bilins) function to stimulate the enzymatic activity of magnesium chelatase (MgCh) via their interaction with GENOMES UNCOUPLED 4 (GUN4) in the model green alga Chlamydomonas reinhardtii A key tetrapyrrole-binding component of MgCh found in all oxygenic photosynthetic species, CrGUN4, also stabilizes the bilin-dependent accumulation of protoporphyrin IX-binding CrCHLH1 subunit of MgCh in light-grown C. reinhardtii cells by preventing its photooxidative inactivation. Exogenous application of biliverdin IXα reverses the loss of CrCHLH1 in the bilin-deficient heme oxygenase (hmox1) mutant, but not in the gun4 mutant. We propose that these dual regulatory roles of GUN4:bilin complexes are responsible for the retention of bilin biosynthesis in all photosynthetic eukaryotes, which sustains chlorophyll biosynthesis in an illuminated oxic environment.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/18h6s0tcTest

المؤلفون: Shi, Weida

مرشدي الرسالة: Marie-Josee Dumont (Internal/Supervisor)

مصطلحات موضوعية: Engineering - Materials Science

العلاقة: Pid: 123319

الإتاحة: https://escholarship.mcgill.ca/concern/theses/s4655k70fTest

المؤلفون: Shi, Weida^1,2 (AUTHOR), Jin, Zhihao¹ (AUTHOR), Wen, Juanjuan¹ (AUTHOR), Chen, Xianfu¹ (AUTHOR), Fu, Kaiyun¹ (AUTHOR), Da, Xiaowei² (AUTHOR), Qiu, Minghui¹ (AUTHOR) qiumh_1201@njtech.edu.cn, Fan, Yiqun¹ (AUTHOR)

المصدر: Journal of the European Ceramic Society. Sep2024, Vol. 44 Issue 11, p6687-6696. 10p.

مصطلحات موضوعية: *RAPID thermal processing, *PORE size distribution, *ULTRAFILTRATION, *WATER purification

مستخلص: The development of tight ceramic ultrafiltration membranes for water purification facilitates is challenging because of high fabrication costs and low permeate flux. In this study, a low-energy rapid thermal process (RTP) was comprehensively investigated for fabricating of high-precision, permeable α-alumina tight ultrafiltration membranes. Compared to the conventional thermal process (CTP), RTP offers an ultra-fast heating rate and shorter holding time, effectively mitigating grain growth and pore aggregation. Moreover, the decomposition of organic matter is delayed during RTP, providing a lower interfacial energy that enables direct formation of α-alumina at a low temperature of 750 °C. During a secondary subsequent RTP, the residual phase is fully converted to α-alumina by the in-situ seeding effect. By utilizing RTP, we successfully prepared α-alumina ultrafiltration membranes within 30 min by calcination, with an average pore size of approximately 6 nm, a narrow pore distribution coefficient of 1.12, and high permeance of 219 L−1·m−2·h−1·bar−1. [ABSTRACT FROM AUTHOR]

المؤلفون: Zhang, Weiqing, Deng, Rui, Shi, Weida, Li, Zheng, Larkin, Robert M., Fan, Qiuling, Duanmu, Deqiang

المصدر: Frontiers in Microbiology ; volume 13 ; ISSN 1664-302X

مصطلحات موضوعية: Microbiology (medical), Microbiology

الوصف: Bilins are open-chain tetrapyrroles synthesized in phototrophs by successive enzymic reactions catalyzed by heme oxygenases (HMOXs/HOs) and ferredoxin-dependent biliverdin reductases (FDBRs) that typically serve as chromophore cofactors for phytochromes and phycobiliproteins. Chlamydomonas reinhardtii lacks both phycobiliproteins and phytochromes. Nonetheless, the activity and stability of photosystem I (PSI) and the catalytic subunit of magnesium chelatase (MgCh) named CHLH1 are significantly reduced and phototropic growth is significantly attenuated in a hmox1 mutant that is deficient in bilin biosynthesis. Consistent with these findings, previous studies on hmox1 uncovered an essential role for bilins in chloroplast retrograde signaling, maintenance of a functional photosynthetic apparatus, and the direct regulation of chlorophyll biosynthesis. In this study, we generated and screened a collection of insertional mutants in a hmox1 genetic background for suppressor mutants with phototropic growth restored to rates observed in wild-type 4A+ C. reinhardtii cells. Here, we characterized a suppressor of hmox1 named ho1su1 with phototrophic growth rates and levels of CHLH1 and PSI proteins similar to 4A+. Tetrad analysis indicated that a plasmid insertion co-segregated with the suppressor phenotype of ho1su1 . Results from TAIL-PCR and plasmid rescue experiments demonstrated that the plasmid insertion was located in exon 1 of the HMOX1 locus. Heterologous expression of the bilin-binding reporter Nostoc punctiforme NpF2164g5 in the chloroplast of ho1su1 indicated that bilin accumulated in the chloroplast of ho1su1 despite the absence of the HMOX1 protein. Collectively, our study reveals the presence of an alternative bilin biosynthetic pathway independent of HMOX1 in the chloroplasts of Chlamydomonas cells.

الإتاحة: https://doi.org/10.3389/fmicb.2022.956554Test

المؤلفون: Shi, Weida, Hu, Xiaoyu, Qiu, Minghui, Jin, Zhihao, Chen, Xianfu, Fan, Yiqun

المصدر: Separation and Purification Technology ; volume 306, page 122524 ; ISSN 1383-5866

مصطلحات موضوعية: Filtration and Separation, Analytical Chemistry

الإتاحة: https://doi.org/10.1016/j.seppur.2022.122524Test
https://api.elsevier.com/content/article/PII:S1383586622020809?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1383586622020809?httpAccept=text/plainTest

المؤلفون: Lyu, Sunjian, Yuan, Xuemei, Zhang, Haiqi, Shi, Weida, Hang, Xiaoying, Liu, Li, Cao, Zheng, Wu, Yinglei

المساهمون: Zhejiang Provincial Science and Technology Program, Zhejiang Provincial Natural Science Foundation

المصدر: Archives of Virology ; volume 164, issue 10, page 2593-2597 ; ISSN 0304-8608 1432-8798

مصطلحات موضوعية: Virology, General Medicine

الإتاحة: https://doi.org/10.1007/s00705-019-04324-8Test

المؤلفون: Zhang, Sijia, Shi, Weida

المصدر: Proceedings of the 5th International Conference on Information Engineering for Mechanics and Materials ; Advances in Engineering Research ; ISSN 2352-5401

الإتاحة: https://doi.org/10.2991/icimm-15.2015.4Test

المؤلفون: Shi, Weida, Yang, Chao, Qiu, Minghui, Chen, Xianfu, Fan, Yiqun

المصدر: Journal of Membrane Science ; volume 642, page 119992 ; ISSN 0376-7388

مصطلحات موضوعية: Filtration and Separation, Physical and Theoretical Chemistry, General Materials Science, Biochemistry

الإتاحة: https://doi.org/10.1016/j.memsci.2021.119992Test
https://api.elsevier.com/content/article/PII:S0376738821009340?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0376738821009340?httpAccept=text/plainTest

المؤلفون: Shi, Weida, Hu, Xiaoyu, Qiu, Minghui, Jin, Zhihao, Chen, Xianfu, Fan, Yiqun

المصدر: SSRN Electronic Journal ; ISSN 1556-5068

الإتاحة: https://doi.org/10.2139/ssrn.4212385Test

المؤلفون: Qi, Ting, Chen, Xianfu, Shi, Weida, Wang, Tao, Qiu, Minghui, Da, Xiaowei, Wen, Juanjuan, Fan, Yiqun

المصدر: Separation and Purification Technology ; volume 278, page 119589 ; ISSN 1383-5866

الإتاحة: https://doi.org/10.1016/j.seppur.2021.119589Test
https://api.elsevier.com/content/article/PII:S1383586621012971?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1383586621012971?httpAccept=text/plainTest