المؤلفون: Mokhlesi, Omalbanin, Sadeghian, Mohammad Hadi, Shajiei, Arezoo, Sheikhi, Maryam, Siyadat, Payam, Kooshyar, Mohammad Mehdi, Rahimi, Hossein, Amini, Nafiseh, Dadgar Moghadam, Maliheh, Ayatollahi, Hossein, Shams, Seyyede Fatemeh, Khoshnegah, Zahra

المصدر: Journal of Advances in Medical and Biomedical Research 31(146) 244-249

مصطلحات موضوعية: T315I Mutation, Chronic Myeloid Leukemia (CML), Imatinib Resistance

الوصف: Background and Objective: Chronic myeloid leukemia (CML) is a myeloproliferative disorder caused by an aberrant BCR-ABL fusion protein. Imatinib mesylate (IM) is a tyrosine kinase inhibitor that induces clinical remissions in chronic-phase CML patients. The T315I mutation at the gatekeeper residues of BCR-ABL confers resistance to both IM and second-generation TKIs, including dasatinib and nilotinib. Our objective was to determine the prevalence of T315I mutation between two groups of CML patients before and during Imatinib treatment in North-East of Iran. Materials and Methods: This study was conducted on 100 newly diagnosed cases of CML (before commencing IM treatment) and 25 IM-resistant CML patients. PCR-RFLP, ASO-PCR, and direct sequencing were performed to detect T315I mutations. Results: The median age of newly-diagnosed and IM-resistant patients was 48±14 and 50±12.3 years, respectively. Males/Females ratio was 1 and 1.08 for newly diagnosed and IM-resistant patients, respectively. There was no significant difference regarding the age and sex between the two groups. During the study, T315I mutational analysis was performed for all 125 patients. The prevalence of T315I mutation was 0% and 4% for newly-diagnosed and IM-resistant patients, respectively. T315I mutation was not detected before IM administration, although it was detected in 1 (4%) among resistant patients who were at least 6-months on IM treatment. Conclusion: These observations suggest that T315I mutation may be categorized as secondary resistance and induce clonal expansion due to BCR/ABL instability. Hence, BCR-ABL mutations are less likely to appear before the onset of treatment, as presented in our study. Keywords: T315I Mutation, Chronic Myeloid Leukemia (CML), Imatinib Resistance

العلاقة: https://zenodo.org/record/8251394Test; https://doi.org/10.30699/jambs.31.146.244Test; oai:zenodo.org:8251394

الإتاحة: https://doi.org/10.30699/jambs.31.146.244Test
https://zenodo.org/record/8251394Test

المؤلفون: Shams, Seyyede Fatemeh, Mehrad-Majd, Hassan

المساهمون: Mashhad University of Medical Sciences

المصدر: Cytokine ; volume 177, page 156538 ; ISSN 1043-4666

مصطلحات موضوعية: Molecular Biology, Hematology, Biochemistry, Immunology, Immunology and Allergy

الإتاحة: https://doi.org/10.1016/j.cyto.2024.156538Test
https://api.elsevier.com/content/article/PII:S1043466624000413?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1043466624000413?httpAccept=text/plainTest

المؤلفون: Shams, Seyyede Fatemeh, Mohammadipour, Mahshid, Deyhim, Mohammad Reza

المساهمون: Iran National Science Foundation

المصدر: Journal of Drug Delivery Science and Technology ; volume 86, page 104644 ; ISSN 1773-2247

مصطلحات موضوعية: Pharmaceutical Science

الإتاحة: https://doi.org/10.1016/j.jddst.2023.104644Test
https://api.elsevier.com/content/article/PII:S1773224723004963?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1773224723004963?httpAccept=text/plainTest

المؤلفون: Shams, Seyyede Fatemeh¹, Mohammadipour, Mahshid¹, Deyhim, Mohammadreza¹ mohamadimahshid213@yahoo.com

المصدر: Nanomedicine Journal. Spring2023, Vol. 10 Issue 2, p131-137. 7p.

مصطلحات موضوعية: *EXOSOMES, *EXTRACELLULAR vesicles, *TRANSMISSION electron microscopy, *STEM cells, *DELIVERY (Obstetrics), *ZETA potential

مستخلص: Objective(s): Exosomes are extracellular vesicles (EV), which are released from the endosomal membrane of the cells. They are 30-120 nm in size, and carry many biological substances such as protein, lipids, and RNA. All kinds of cells release exosomes during their life. Exosomes are a small showcase of the mother cell's contents. Platelets-derived exosomes (Exo-Plt) are the most frequent nanoscale particles in the peripheral blood. Their availability and non-toxicity as an autologous carrier and a growth factor cargo made us introduce the most efficient method to isolate exosomes from platelet products and also examine their ability as a delivery system. Materials and Methods: Two different protocols including stimulated and unstimulated were used in the present study to obtain Exo-Plt. Dynamic light scattering (DLS), BCA assay, Transmission Electron microscopy (TEM), and western blotting were performed to assess the basic characteristics (size and zeta potential, protein content, morphology, and CD markers, respectively) of the isolated particles. Consequently, the ability of Exo-plts for delivery of exogenous miRNA into the model cell line was assessed by Real-Time PCR. Results: The stimulated method was more efficient to isolate Exo-Plt. Additionally, the applied agonist and the time of sample preparation from individuals affected the exosome yield. The Exo-Plts prosperously delivered the exogenous cargo into the cell line. Conclusion: This research provided a standard protocol to isolate high-quality and pure Exo-Plt for biocompound delivery and even therapeutic objectives. Exosomes provide the opportunity for cell-free therapy. [ABSTRACT FROM AUTHOR]

المؤلفون: Foomani, Farzad Mollahoseini, Sadeghian, Mohammad Hadi, Bagheri, Saeede, Badiee, Zahra, Bazargani, Reihane, Aryanpour, Zahra, Hallajiayan, Saeid, Shams, Seyyede Fatemeh

المصدر: International Journal of Hematology-Oncology & Stem Cell Research; Jan2023, Vol. 17 Issue 1, p4-8, 5p

مصطلحات موضوعية: BLOOD group antigens, ABO blood group system, BLOOD transfusion reaction, BLOOD groups, GENETIC disorders, BLOOD transfusion

مصطلحات جغرافية: IRAN

مستخلص: Background: Thalassemia is an inherited disease with anemia and hemolysis. Blood transfusion is a routine treatment for thalassemia patients; alloimmunization is one of the complications of blood transfusion, which is very serious for these patients, especially girls and young women. Materials and Methods: In this cross-sectional study 446 thalassemia patients were examined. Demographic information of patients was extracted and recorded. The phenotype of ABO, Rh and Kell antigens (tube method) with antisera from IMMUNDIANOSTICA Company (Germany) and frequency of alloantibodies was determined. Results: 55.8% of studied individuals were male and 44.2% were female. Mean age of studied patients was 19.94±10.63. The alloantibodies were detected in 7.5% of pack cell receivers. The most prevalent phenotype of ABO system was O blood group (37.4%), and the most abundant antigen of Rh group was 'e', which was found in 99.8% of studied population. The most frequent detected alloantibody was Anti K (38.2%); concerning kell phenotype, (K_k+) and (K+k+) were found in 99.3% and 0.7% of patients, respectively. The frequency of Anti-D, Anti-C, Anti-c and Anti-E was 23.5%, 14.7%, 2.9% and 14.7%, respectively. Conclusion: According to the results of this paper, finding the compatible pack cells in terms of Kell and Rh systems antigens in addition to ABO blood group is recommended to decrease the rate of alloantibodies in thalassemia patients. [ABSTRACT FROM AUTHOR]

: Copyright of International Journal of Hematology-Oncology & Stem Cell Research is the property of Tehran University of Medical Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Shakeri, Sepideh, Soltani, Narjes, Java, Mohammad Reza, Abdolalian, Mehrnaz, Ayatollahi, Hossein, Shams, Seyyede Fatemeh

المصدر: Medical Laboratory Journal; Jan/Feb2023, Vol. 17 Issue 1, p42-46, 5p

مصطلحات موضوعية: FANCONI'S anemia, REGULATOR genes, MITOMYCIN C, DISEASE susceptibility, SYMPTOMS, RECESSIVE genes

مصطلحات جغرافية: IRAN

مستخلص: Background and objectives: Fanconi anemia (FA) is an autosomal recessive disorder that usually manifest in forms of pancytopenia, hyperpigmentation, and skeletal complications. Mutation in the DNA repair regulatory genes is associated with the development of FA. Examination of chromosomal breakages when chromosomes are exposed to cross-linking agents is a common method of FA diagnosis. This study aimed to evaluate the prevalence and characteristics of patients with FA in Mashhad, northeast of Iran. Methods: In this study, we evaluated 312 suspected FA patients who had been referred to the laboratory of Ghaem Hospital during 2014-2020. The mitomycin C method was used to identify FA-positive subjects. Results: After the examinations, 84 patients (26.9%) were cytogenetically positive for FA. Of 84 patients, 48 (57.1%) were male and 36 (42.9%) were female. Thumb abnormality was the most common congenital anomaly (43.2%). Conclusion: Based on the findings, males are more susceptible to FA, and thumb abnormality is the most common congenital anomaly associated with FA. Combination of clinical manifestations and genetic susceptibility in patients may contribute to a more accurate diagnosis. [ABSTRACT FROM AUTHOR]

: Copyright of Medical Laboratory Journal is the property of Golestan University of Medical Sciences, Deputy of Research & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Yazdandoust, Ehsan, Sadeghian, Mohammad Hadi, Shams, Seyyede Fatemeh, Ayatollahi, Hossein, Saadatpour, Yasaman, Siyadat, Payam, Sheikhi, Maryam, Afzalaghaee, Monavvar

المصدر: Iranian Journal of Pathology; Fall2022, Vol. 17 Issue 4, p419-426, 8p

مصطلحات موضوعية: GENE expression, ACUTE myeloid leukemia, MOLECULAR pathology, HEMATOLOGIC malignancies, GENETIC mutation, BONE marrow examination

مصطلحات جغرافية: IRAN

مستخلص: Background & Objective: Acute myeloid leukemia (AML) is a hematopoietic malignancy caused by genetic abnormalities. Currently, molecular and genetic factors are routinely used as diagnostic and prognostic markers. FLT-3 is one of the most known diagnostic factors in AML. MDR1 gene belongs to the ATP binding cassette family; it is known as one of the chemotherapy-resistant causes of AML. We aimed to study FLT-3ITD mutations and their association with MDR1 gene expression in AML individuals. Methods: For investigation, 80 AML individuals and 20 healthy controls were selected. This study was done in the Cancer molecular Pathology Research Center of Mashhad University of Medical Sciences (MUMS), Iran during 2017-2019. FLT3-ITD mutation was assessed by polymerase chain reaction (PCR); Real-time quantitative PCR was performed to measure the amount of MDR1 gene expression. Bone marrow and blood smears of patients were evaluated in terms of morphology. SPSS 16.0 was used for data analysis. Results: FLT3-ITD mutation and MDR1 overexpression were found in 18.8% and 23.8% of AML patients, respectively. Statistical analysis did not show any relationship or association between these two markers. Cuplike morphology was observed in blast cells in 21.25% of AML cases, which was associated with the presence of FLT3-ITD mutation. Conclusion: FLT-3 and MDR1 function independently. Survival studies to determine the exact role of MDR1 overexpression in drug resistance issues would be suggested. [ABSTRACT FROM AUTHOR]

: Copyright of Iranian Journal of Pathology is the property of Iranian Society of Pathology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Shakeri, Sepideh, Ayatollahi, Hossein, Sadeghian, Mohammad Hadi, Shams, Seyyede Fatemeh

المصدر: Middle East Journal of Cancer; Jul2021, Vol. 12 Issue 3, p391-398, 8p

مصطلحات موضوعية: MYELODYSPLASTIC syndromes, LYMPHOBLASTIC leukemia, CROSS-sectional method, AGE distribution, ACUTE myeloid leukemia, MOLECULAR pathology, KARYOTYPES, HEMATOLOGIC malignancies, CHROMOSOME abnormalities, BONE marrow, CYTOGENETICS, DATA analysis software

مصطلحات جغرافية: IRAN

مستخلص: Background: Chromosomal aberrations which occur in different hematologic malignancies are believed to be highly applicable for identifying the prognosis and treatment protocols. We conducted the present study to investigate the chromosomal and molecular abnormalities in bone marrow of acute myeloid leukemia (AML), acute lymphoid leukemia (ALL), and myelodysplastic syndrome patients. Method: We performed this cross-sectional study in molecular pathology and cancer research center of Mashhad University of Medical Sciences (MUMS), during 2017-2019; the total number of our cases was 252. We did all the molecular and cytogenetic tests on these patients. SPSS V.16 software was utilized for the analysis of our data. Results: In this research, the ALL patients were meaningfully younger than AML ones. There were significant associations between karyotype patterns and types of malignancy; normal diploid was more frequent in myelodysplastic syndrome (P<0.05). Among numerical abnormalities, trisomy 3 and monosomy 14 were the most prevalent ones. Conclusion: The results of similar studies from different areas with different ethnics would help to identify new parameters for prognosis determination. Cytogenetic analysis is highly applicable for leukemia diagnosis and prognosis. [ABSTRACT FROM AUTHOR]

: Copyright of Middle East Journal of Cancer is the property of Middle East Journal of Cancer and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Z. Arianpour, F Molahosseini Foomani, Shams Seyyede Fatemeh, Noorin H.Gh.

المصدر: Iranian Journal of Virology. 8:1-6

مصطلحات موضوعية: medicine.diagnostic_test, business.industry, Human immunodeficiency virus (HIV), medicine.disease_cause, medicine.disease, Virology, Virus, Immune system, Positive HIV, Western blot, Acquired immunodeficiency syndrome (AIDS), Elisa test, Immunology, medicine, business, Indeterminate

الوصف: Background and Aims: HIV is spreading rapidly among people word wide. Infection with this virus leads to immune suppression and finally acquired immune deficiency syndrome (AIDS).Early HIV detection is depended on antibody screening against virus by Enzyme Linked Immunosorbent Assay (ELISA).Some confirmatory tests such as; Western Blot and Recombinant Immunobloting Assay (RIBA), are used to verify viral infection. Many of confirmatory tests results are indeterminate. The aim of this study is comparing the frequency and patterns of indeterminate results in two groups; blood donors and patients with high risk behaviors, in northeast of Iran. Materials and Methods: From October 2009 to March 2014 total number of 1055 serum samples with previous positive HIV ELISA test history, were tested in our laboratory. Some by RIBA and some by western blot method. Results: Most of indeterminate results belonged to blood donors that were tested by Western Blot analysis and were positive. The most reacting band was P24 in both methods and groups. Conclusion: RIBA assay is more sensitive and reliable than western Blot; but it’s necessary to use other supplementary tests with less indistinctive results.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::b2225f9f7e61acd22200595903adcde3Test
https://doi.org/10.21859/isv.8.1.1Test

المؤلفون: Sadeghian, Mohammad-Hadi, Ayatollahi, Hosein, Shams, Seyyede-Fatemeh, Dezaki, Zahra Rezaei

المصدر: Middle East Journal of Cancer; Oct2019, Vol. 10 Issue 4, p305-313, 9p, 3 Charts, 2 Graphs

مصطلحات موضوعية: BONE marrow examination, COMPARATIVE studies, GENE expression, GENETIC mutation, MOLECULAR pathology, POLYMERASE chain reaction, TRANSCRIPTION factors, DNA-binding proteins, ACUTE myeloid leukemia, CASE-control method, DESCRIPTIVE statistics

مصطلحات جغرافية: IRAN