المؤلفون: Guerini C., Furlan D., Ferrario G., Grillo F., Libera L., Arpa G., Klersy C., Lenti M. V., Riboni R., Solcia E., Fassan M., Mastracci L., Ardizzone S., Moens A., De Hertogh G., Ferrante M., Graham R. P., Sessa F., Paulli M., Di Sabatino A., Vanoli A.

المساهمون: Guerini, C., Furlan, D., Ferrario, G., Grillo, F., Libera, L., Arpa, G., Klersy, C., Lenti, M. V., Riboni, R., Solcia, E., Fassan, M., Mastracci, L., Ardizzone, S., Moens, A., De Hertogh, G., Ferrante, M., Graham, R. P., Sessa, F., Paulli, M., Di Sabatino, A., Vanoli, A.

مصطلحات موضوعية: immune-mediated disorder, non-conventional dysplasia, small intestinal carcinoma

الوصف: Aims: Patients with Crohn's disease (CrD) have an elevated risk for the development of small bowel adenocarcinomas (SBAs). Actionable isocitrate dehydrogenase 1 (IDH1) mutations have been reported to be more frequent in CrD-SBAs than in sporadic SBAs. The present study aimed to investigate the clinicopathological and immunophenotypical features, as well as methylation profiles, of IDH1-mutated CrD-SBAs. Methods and results: An international multicentre series of surgically resected CrD-SBAs was tested for IDH1 mutation. Clinicopathological features, immunophenotypical marker expression and O6-methylguanine-DNA methyltransferase (MGMT) and long interspersed nuclear element-1 (LINE-1) methylation were compared between IDH1-mutated and IDH1 wild-type CrD-SBAs. Ten (20%) of the 49 CrD-SBAs examined harboured an IDH1 mutation and all the mutated cancers harboured the R132C variant. Compared to IDH1 wild-type cases, IDH1-mutated CrD-SBAs showed significantly lower rates of cytokeratin 7 expression (P = 0.005) and higher rates of p53 overexpression (P = 0.012) and MGMT methylation (P = 0.012). All three dysplastic growths associated with IDH1-mutated SBAs harboured the same IDH1 variant (R132C) of the corresponding invasive cancer, and all were of non-conventional subtype (two serrated dysplastic lesions and one goblet cell-deficient dysplasia). In particular, non-conventional serrated dysplasia was significantly associated with IDH1-mutated CrD-SBAs (P = 0.029). No significant cancer-specific survival difference between IDH1-mutated CrD-SBA patients and IDH1 wild-type CrD-SBA patients was found (hazard ratio = 0.55, 95% confidence interval = 0.16–1.89; P = 0.313). Conclusions: IDH1-mutated CrD-SBAs, which represent approximately one-fifth of total cases, are characterised by distinctive immunophenotypical features and methylation profiles, with potential therapeutic implications. Moreover, IDH1-mutated non-conventional, serrated dysplasia is likely to represent a precursor lesion to such CrD-SBAs.

وصف الملف: ELETTRONICO

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/37988281; info:eu-repo/semantics/altIdentifier/wos/WOS:001107860200001; volume:84; issue:3; firstpage:515; lastpage:524; numberofpages:10; journal:HISTOPATHOLOGY; https://hdl.handle.net/11383/2167252Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85177449323

الإتاحة: https://doi.org/10.1111/his.15095Test
https://hdl.handle.net/11383/2167252Test

المؤلفون: Zuccarello, P., Carnazza, G., Salerno, M., Esposito, M., Cosentino, S., Giorlandino, A., Sessa, F., Pomara, C., Barbera, N.

المساهمون: Università degli Studi di Catania

المصدر: International Journal of Legal Medicine ; ISSN 0937-9827 1437-1596

مصطلحات موضوعية: Pathology and Forensic Medicine

الوصف: Food aspiration is one of the major health risks for elderly people in nursing homes which could lead to death. Moreover, misconducts in pharmacotherapy may represent a potential risk of adverse drug reactions. It is reported here the toxicological evaluation of a combined death by food aspiration and acute escitalopram intoxication of a psychiatric subject, occurred in a nursing home. An 89-year-old man, suffering from dysphagia and Alzheimer’s, was resident in a nursing home. He was fed with a liquid diet administered directly in mouth using a syringe. The man was also being treated with escitalopram 10 mg tablet. One evening, after receiving the meal in the usual way, the man complained of sudden illness. Carried to the emergency room, the man died about 3 h later with a diagnosis of cardiogenic shock subsequentially to ab ingestis. The histological findings revealed the presence of exogenous material, probably food, up to the finest bronchial branches. The toxicological examination revealed the presence of escitalopram and its main metabolite, desmethylcitalopram: in the blood 1972 ng/ml and 285 ng/ml, in the brain 4657 ng/g and 1025 ng/g, in the gastric content 2317 ng/g and 423 ng/g, in the lung 21,771 ng/g and 468 ng/g, respectively. The bad practice of the nurses to dissolve the escitalopram tablet in the liquefied food and to administer the therapy with a syringe directly into the mouth emerged thanks this investigation. Following food aspiration, escitalopram was absorbed by inhalation route, reaching high concentrations in blood and tissues. The death occurred due to a combined mechanism between food aspiration and the escitalopram toxic action.

الإتاحة: https://doi.org/10.1007/s00414-024-03168-5Test

المؤلفون: Ji M., Giangieri G., Usman M., Liu C., Bosaro M., Sessa F., Canu P., Treu L., Campanaro S.

المساهمون: Ji, M., Giangieri, G., Usman, M., Liu, C., Bosaro, M., Sessa, F., Canu, P., Treu, L., Campanaro, S.

مصطلحات موضوعية: Plasticizers, Magnetic biochar, Degradation, Metagenome, Pangenome

الوصف: There has been growing concern over the release of plasticizers from plastic products, and the high levels of plasticizers in the environment have led to a threat to ecological security. Although some plasticizers may naturally degrade, their slow removal and prolonged life cycle remain challenges. To address this, this study explored a unique hybrid strategy using native field microorganisms and magnetic biochar (MBC) to support the upstream degradation of plasticizers. Diethyl phthalate (DP) was used as the test subject. The study found that MBC treatment led to high level of total organic carbon (TOC) and various organic products, demonstrating the degradation of DP. Analysis of the hybrid metagenomic model showed that several species of Pseudomonas can degrade downstream phenylmethanal and Pseudomonas nitroreducens has the ability to cooperate well with MBC due to its iron receptor and transporter. Additionally, a Pigmentiphaga species was found to have the ability to fully mineralize DP. Analysis of the Pigmentiphaga pangenome revealed that genes related to DP biodegradation were shared by members of this genus. Although some members of Pseudomonas is known to be pathogenic, the species identified in the study may not be harmful as they lack virulence factors. The study provides evidence regarding the cooperation between native biodegraders and MBC in mineralizing plasticizers, offering a new solution for removing phthalate plasticizers from soil and surface water.

وصف الملف: ELETTRONICO

العلاقة: info:eu-repo/semantics/altIdentifier/wos/WOS:001009347800001; volume:468; firstpage:143589; journal:CHEMICAL ENGINEERING JOURNAL; https://hdl.handle.net/11577/3484270Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85159596346; https://www.sciencedirect.com/science/article/pii/S1385894723023203Test

الإتاحة: https://doi.org/10.1016/j.cej.2023.143589Test
https://hdl.handle.net/11577/3484270Test
https://www.sciencedirect.com/science/article/pii/S1385894723023203Test

المؤلفون: Piacentino F., Fontana F., Zorzetto G., Saccomanno A., Gatta T., Recaldini C., Franzi F., Imperatori A., Rotolo N., Coppola A., Minenna M., Minici R., Ascenti V., Tripodi G., Bottari A., Lagana D., Ierardi A. M., Carrafiello G., Sessa F., Carcano G., Ascenti G., Venturini M.

المساهمون: Piacentino, F., Fontana, F., Zorzetto, G., Saccomanno, A., Gatta, T., Recaldini, C., Franzi, F., Imperatori, A., Rotolo, N., Coppola, A., Minenna, M., Minici, R., Ascenti, V., Tripodi, G., Bottari, A., Lagana, D., Ierardi, A. M., Carrafiello, G., Sessa, F., Carcano, G., Ascenti, G., Venturini, M.

مصطلحات موضوعية: cone beam CT, fusion imaging, percutaneous biopsy, spectral CT, Z-effective images

الوصف: The aim of the study was to try to obtain more information on diagnostic samplings and biomarkers using dual-layer spectral CT in lung biopsies. Lung biopsies were performed by merging images obtained with CBCT with those from spectral CT to use them as functional guidance, experimenting with double sampling to determine the difference between the area with a higher Z-effective number and that with a lower Z-effective number. Ten patients with large lung lesions on spectral CT were selected and underwent percutaneous transthoracic lung mass biopsy. Technical success was calculated. The percentage of neoplastic, inflammatory, fibrotic, necrotic cells, or non-neoplastic lung parenchyma was reported. The possibility of carrying out immunohistochemical or molecular biology investigations was analyzed. All lesions were results malignant in 10/10 samples in the Zmax areas; in the Zmin areas, malignant cells were found in 7/10 samples. Technical success was achieved in 100% of cases for Zmax sampling and in 70% for Zmin sampling (p-value: 0.2105). The biomolecular profile was detected in 9/10 (90%) cases in Zmax areas, while in 4/10 (40%) cases in Zmin areas (p-value: 0.0573). The advantage of Z-effective imaging would be to identify a region of the lesion that is highly vascularized and probably richer in neoplastic cells, thus decreasing the risk of obtaining a non-diagnostic biopsy sample.

وصف الملف: ELETTRONICO

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/38068478; info:eu-repo/semantics/altIdentifier/wos/WOS:001116035200001; volume:12; issue:23; firstpage:1; lastpage:14; numberofpages:14; journal:JOURNAL OF CLINICAL MEDICINE; https://hdl.handle.net/11383/2166858Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85179355309

الإتاحة: https://doi.org/10.3390/jcm12237426Test
https://hdl.handle.net/11383/2166858Test

المؤلفون: Agaimy A., Kasajima A., Stoehr R., Haller F., Schubart C., Togel L., Pfarr N., von Werder A., Pavel M. E., Sessa F., Uccella S., La Rosa S., Kloppel G.

المساهمون: Agaimy, A., Kasajima, A., Stoehr, R., Haller, F., Schubart, C., Togel, L., Pfarr, N., von Werder, A., Pavel, M. E., Sessa, F., Uccella, S., La Rosa, S., Kloppel, G.

مصطلحات موضوعية: ACTH, Neuroendocrine tumor, NET, ectopic Cushing, EWSR1, BCOR fusion, BEND2 fusion, KMT2A

الوصف: Ectopic Cushing syndrome is a rare clinical disorder resulting from excessive adrenocorticotrophic hormone (ACTH) produced by non-pituitary neoplasms, mainly neuroendocrine neoplasms (NENs) of the lung, pancreas, and gastrointestinal tract, and other less common sites. The genetic background of ACTH-producing NENs has not been well studied. Inspired by an index case of ACTH-producing pancreatic NEN carrying a gene fusion, we postulated that ACTH-producing NENs might be enriched for gene fusions. We herein examined 21 ACTH-secreting NENs of the pancreas (10), lung (9), thymus (1), and kidney (1) using targeted RNA sequencing. The tumors were classified according to the most recent WHO classification as NET-G1/typical carcinoid (n = 4), NETG-2/atypical carcinoid (n = 14), and NET-G3 (n = 3). Overall, targeted RNA sequencing was successful in 11 cases (4 of 10 pancreatic tumors, 5 of 9 pulmonary tumors, and in the one renal and one thymic tumor). All four successfully tested pancreatic tumors revealed a gene fusion: two had a EWSR1::BEND2 and one case each had a KMT2A::BCOR and a TFG::ADGRG7 fusion, respectively. EWSR1 rearrangements were confirmed in both tumors with a EWSR1::BEND2 by FISH. Gene fusions were mutually exclusive with ATRX, DAXX, and MEN1 mutations (the most frequently mutated genes in NETs) in all four cases. Using RNA-based variant assessment (n = 16) or via the TSO500 panel (n = 5), no pathogenic BCOR mutations were detected in any of the cases. Taken together, gene fusions were detected in 4/4 (100%) pancreatic versus 0/7 (0%) non-pancreatic tumors, respectively. These results suggest a potential role for gene fusions in triggering the ACTH production in pancreatic NENs presenting with ectopic Cushing syndrome. While the exact mechanisms responsible for the ectopic ACTH secretion are beyond the scope of this study, overexpressed fusion proteins might be involved in promoter-mediated overexpression of pre-ACTH precursors in analogy to the mechanisms postulated for EWSR1::CREB1-mediated ...

وصف الملف: ELETTRONICO

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/36690805; info:eu-repo/semantics/altIdentifier/wos/WOS:000921702600001; volume:482; firstpage:507; lastpage:516; numberofpages:10; journal:VIRCHOWS ARCHIV; https://hdl.handle.net/11383/2146623Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85146694080

الإتاحة: https://doi.org/10.1007/s00428-022-03484-4Test
https://hdl.handle.net/11383/2146623Test

المؤلفون: Cardia A., Epistolio S., Zaed I., Sahnane N., Cerutti R., Cipriani D., Barizzi J., Spina P., Stefanini F. M., Cerati M., Balbi S., Mazzucchelli L., Sessa F., Pesce G. A., Reinert M., Frattini M., Marchi F.

المساهمون: Cardia, A., Epistolio, S., Zaed, I., Sahnane, N., Cerutti, R., Cipriani, D., Barizzi, J., Spina, P., Stefanini, F. M., Cerati, M., Balbi, S., Mazzucchelli, L., Sessa, F., Pesce, G. A., Reinert, M., Frattini, M., Marchi, F.

مصطلحات موضوعية: MGMT, glioblastoma, miRNA, overall survival, progression-free survival, temozolomide

الوصف: Glioblastoma multiforme (GBM) remains one of the tumors with the worst prognosis. In recent years, a better overall survival (OS) has been described in cases subjected to Gross Total Resection (GTR) that were presenting hypermethylation of Methylguanine-DNA methyltransferase (MGMT) promoter. Recently, also the expression of specific miRNAs involved in MGMT silencing has been related to survival. In this study, we evaluate MGMT expression by immunohistochemistry (IHC), MGMT promoter methylation and miRNA expression in 112 GBMs and correlate the data to patients' clinical outcomes. Statistical analyses demonstrate a significant association between positive MGMT IHC and the expression of miR-181c, miR-195, miR-648 and miR-767.3p between unmethylated cases and the low expression of miR-181d and miR-648 and between methylated cases and the low expression of miR-196b. Addressing the concerns of clinical associations, a better OS has been described in presence of negative MGMT IHC, in methylated patients and in the cases with miR-21, miR-196b overexpression or miR-767.3 downregulation. In addition, a better progression-free survival (PFS) is associated with MGMT methylation and GTR but not with MGMT IHC and miRNA expression. In conclusion, our data reinforce the clinical relevance of miRNA expression as an additional marker to predict efficacy of chemoradiation in GBM.

وصف الملف: ELETTRONICO

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/36902848; info:eu-repo/semantics/altIdentifier/wos/WOS:000947107200001; volume:12; issue:5; firstpage:1; lastpage:13; numberofpages:13; journal:JOURNAL OF CLINICAL MEDICINE; https://hdl.handle.net/11383/2167860Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85149981266

الإتاحة: https://doi.org/10.3390/jcm12052061Test
https://hdl.handle.net/11383/2167860Test

المؤلفون: Didier HA, Cappellari AM, Sessa F, Giannì AB, Didier AH, Pavesi MM, Caria MP, Curone M, Tullo V, Di Berardino F, Iacona E, Lilli G, Barozzi S, Aldè M, De Bortoli G, Zanetti D, Arnone F, Bussone G.

المساهمون: H. Didier, A. Cappellari, F. Sessa, A. Giannì, A. Didier, M. Pavesi, M. Caria, M. Curone, V. Tullo, F. Di Berardino, E. Iacona, G. Lilli, S. Barozzi, M. Aldè, G. De Bortoli, D. Zanetti, F. Arnone, G. Bussone

مصطلحات موضوعية: audiology, bruxism, dentistry, somatosensory disorder, temporomandibular joint disorder, tinnitu, Settore MED/29 - Chirurgia Maxillofacciale, Settore MED/32 - Audiologia, Settore MED/28 - Malattie Odontostomatologiche

الوصف: Background: Although the association between tinnitus and temporo-mandibular disorders (TMD) has been frequently reported, their rate of association in the literature shows a great variability. Objective: We aimed to investigate the prevalence of TMD in patients with somatosensory tinnitus and, vice versa, the occurrence of somatosensory tinnitus in patients with TMD. Methods: The study included patients with somatosensory tinnitus (audiological group) and patients with TMD (stomatological group), evaluated at the audiologic and stomatologic clinics of the Policlinic Hospital of Milan, Italy. Common causes of tinnitus, such as hearing and neurological disorders, were excluded. A cervicogenic somatic tinnitus was also ruled out. Different TMD symptoms, including joint noise and joint pain, were considered. The collected data were analysed using descriptive statistical methods, and the Pearson's Chi-squared test was performed to study the prevalence of the different symptoms by clinical groups. Results: Audiological group included 47 patients with somatosensory tinnitus. Overall, TMD was diagnosed in 46 patients (97.8%), including TMJ noise in 37 (78.7%), clenching in 41 (87.2%) and pain in 7 (14.8%) patients. Stomatological group included 50 patients with TMD, including joint noise in 32 (64.0%), clenching in 28 (56.0%) and TMJ pain in 42 (84.0%) patients. A somatosensory tinnitus was diagnosed in 12 (24.0%) patients. Conclusion: Our study showed a high prevalence of TMD in patients with tinnitus, as well as a not uncommon occurrence of tinnitus in patients presenting with TMD. The distribution of TMD symptoms, such as joint noise, and joint pain was different between the two groups.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/37335244; info:eu-repo/semantics/altIdentifier/wos/WOS:001017677200001; firstpage:1; lastpage:4; numberofpages:4; journal:JOURNAL OF ORAL REHABILITATION; https://hdl.handle.net/2434/992128Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85163340058

الإتاحة: https://doi.org/10.1111/joor.13541Test
https://hdl.handle.net/2434/992128Test

المؤلفون: Di Matteo, A, Belloni, E, Pradella, D, Chiaravalli, AM, Pini, GM, Bugatti, M, Alfieri, R, Barzan, C, Franganillo Tena, E, Bione, S, Terenzani, E, Sessa, F, Wyatt, CDR, Vermi, W, Ghigna, C

المصدر: International Journal of Molecular Sciences , 24 (9) , Article 8102. (2023)

مصطلحات موضوعية: RNA binding proteins, alternative splicing, angiogenesis, cancer biomarkers, gastric cancer, tumor vasculature, Up-Regulation, Endothelial Cells, Stomach Neoplasms, Neovascularization, Pathologic, Humans, Male, Female, Adult, Middle Aged, Aged, 80 and over, Biomarkers, Prognosis, Cells, Cultured, Animals, Mice

الوصف: Angiogenesis is crucial for cancer progression. While several anti-angiogenic drugs are in use for cancer treatment, their clinical benefits are unsatisfactory. Thus, a deeper understanding of the mechanisms sustaining cancer vessel growth is fundamental to identify novel biomarkers and therapeutic targets. Alternative splicing (AS) is an essential modifier of human proteome diversity. Nevertheless, AS contribution to tumor vasculature development is poorly known. The Neuro-Oncological Ventral Antigen 2 (NOVA2) is a critical AS regulator of angiogenesis and vascular development. NOVA2 is upregulated in tumor endothelial cells (ECs) of different cancers, thus representing a potential driver of tumor blood vessel aberrancies. Here, we identified novel AS transcripts generated upon NOVA2 upregulation in ECs, suggesting a pervasive role of NOVA2 in vascular biology. In addition, we report that NOVA2 is also upregulated in ECs of gastric cancer (GC), and its expression correlates with poor overall survival of GC patients. Finally, we found that the AS of the Rap Guanine Nucleotide Exchange Factor 6 (RapGEF6), a newly identified NOVA2 target, is altered in GC patients and associated with NOVA2 expression, tumor angiogenesis, and poor patient outcome. Our findings provide a better understanding of GC biology and suggest that AS might be exploited to identify novel biomarkers and therapeutics for anti-angiogenic GC treatments.

وصف الملف: text

العلاقة: https://discovery.ucl.ac.uk/id/eprint/10179580/1/ijms-24-08102.pdfTest; https://discovery.ucl.ac.uk/id/eprint/10179580Test/

الإتاحة: https://discovery.ucl.ac.uk/id/eprint/10179580/1/ijms-24-08102.pdfTest
https://discovery.ucl.ac.uk/id/eprint/10179580Test/

المؤلفون: Mariotti, A., Spatafora, P., Sessa, F., Saieva, C., Galli, I.C., Roviello, G., Doni, L., Zaccaro, C., Bisegna, C., Conte, F.L., Mariottini, R., Marzocco, A., Masieri, L., Vignolini, G., Minervini, A., Serni, S., Carini, M., Nesi, G., Villari, D.

المصدر: European Journal of Surgical Oncology ; volume 49, issue 10, page 107034 ; ISSN 0748-7983

مصطلحات موضوعية: Oncology, General Medicine, Surgery

الإتاحة: https://doi.org/10.1016/j.ejso.2023.107034Test
https://api.elsevier.com/content/article/PII:S0748798323006728?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0748798323006728?httpAccept=text/plainTest

المؤلفون: Campi, R., Pecoraro, A., Sebastianelli, A., Spatafora, P., Sessa, F., Gallo, M.L., Territo, A., Li Marzi, V., Gacci, M., Vignolini, G., Decaestecke, K., Breda, A., Serni, S.

المصدر: European Urology Open Science ; volume 55, page S183 ; ISSN 2666-1683

مصطلحات موضوعية: Urology

الإتاحة: https://doi.org/10.1016/s2666-1683Test(23)00541-4
https://api.elsevier.com/content/article/PII:S2666168323005414?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2666168323005414?httpAccept=text/plainTest