المؤلفون: Seo, Yohan, Lee, Sion, Kim, Minuk, Kim, Dongguk, Jeong, Sung Baek, Das, Raju, Sultana, Armin, Park, SeonJu, Nhiem, Nguyen Xuan, Huong, Phan Thi Thanh, Kwon, Oh-Bin, Namkung, Wan, Woo, Joohan

المصدر: Frontiers in Pharmacology ; volume 15 ; ISSN 1663-9812

مصطلحات موضوعية: Pharmacology (medical), Pharmacology

الوصف: Background: Prostate cancer and non-small cell lung cancer (NSCLC) present significant challenges in the development of effective therapeutic strategies. Hormone therapies for prostate cancer target androgen receptors and prostate-specific antigen markers. However, treatment options for prostatic small-cell neuroendocrine carcinoma are limited. NSCLC, on the other hand, is primarily treated with epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors but exhibits resistance. This study explored a novel therapeutic approach by investigating the potential anticancer properties of vitekwangin B, a natural compound derived from Vitex trifolia . Methods: Vitekwangin B was chromatographically isolated from the fruits of V. trifolia . ANO1 protein levels in prostate cancer and NSCLC cells were verified and evaluated again after vitekwangin B treatment. Results: Vitekwangin B did not inhibit anoctamin1 (ANO1) channel function but significantly reduced ANO1 protein levels. These results demonstrate that vitekwangin B effectively inhibited cancer cell viability and induced apoptosis in prostate cancer and NSCLC cells. Moreover, it exhibited minimal toxicity to liver cells and did not affect hERG channel activity, making it a promising candidate for further development as an anticancer drug. Conclusion: Vitekwangin B may offer a new direction for cancer therapy by targeting ANO1 protein, potentially improving treatment outcomes in patients with prostate cancer and NSCLC. Further research is needed to explore its full potential and overcome existing drug resistance challenges.

الإتاحة: https://doi.org/10.3389/fphar.2024.1382787Test

المؤلفون: Lee, Heejin, Kim, Hye‐Ji, Choi, Dong‐Kyu, Ko, Eu n.‐A., Choi, Jae‐Hyeog, Seo, Yohan, Lee, Sion, Kim, Soong‐Hyun, Jung, Sejin, Kim, Minwoo, Kang, Dongwan, Im, Chun‐Young, Bae, Gi‐Hun, Jung, Sung‐Cherl, Kwon, Oh‐Bin

المصدر: Pharmacology Research & Perspectives ; volume 11, issue 5 ; ISSN 2052-1707 2052-1707

الوصف: The importance of vesicular monoamine transporter 2 (VMAT2) in dopamine regulation, which is considered crucial for neuropsychiatric disorders, is currently being studied. Moreover, the development of disease treatments using histone deacetylase (HDAC) inhibitors (HDACi) is actively progressing in various fields. Recently, research on the possibility of regulating neuropsychiatric disorders has been conducted. In this study, we evaluated whether VMAT2 expression increased by an HDACi can fine‐tune neuropsychotic behavior, such as attention deficit hyperactivity disorder (ADHD) and protect against the cell toxicity through oxidized dopamine. First, approximately 300 candidate HDACi compounds were added to the SH‐SY5Y dopaminergic cell line to identify the possible changes in the VMAT2 expression levels, which were measured using quantitative polymerase chain reaction. The results demonstrated, that treatment with pimelic diphenylamide 106 (TC‐H 106), a class I HDACi, increased VMAT2 expression in both the SH‐SY5Y cells and mouse brain. The increased VMAT2 expression induced by TC‐H 106 alleviated the cytotoxicity attributed to 6‐hydroxydopamine (6‐OHDA) or 1‐methyl‐4‐phenylpyridinium (MPP + ) and free dopamine treatment. Moreover, dopamine concentrations, both intracellularly and in the synaptosomes, were significantly elevated by increased VMAT2 expression. These results suggest that dopamine concentration regulation by VMAT2 expression induced by TC‐H 106 could alter several related behavioral aspects that was confirmed by attenuation of hyperactivity and impulsivity, which were major characteristics of animal model showing ADHD‐like behaviors. These results indicate that HDACi‐increased VMAT2 expression offers sufficient protections against dopaminergic cell death induced by oxidative stress. Thus, the epigenetic approach could be considered as therapeutic candidate for neuropsychiatric disease regulation.

الإتاحة: https://doi.org/10.1002/prp2.1135Test

المؤلفون: Park, SeonJu, Das, Raju, Nhiem, Nguyen Xuan, Jeong, Sung Baek, Kim, Minuk, Kim, Dongguk, Oh, Hye In, Cho, Su-Hyeon, Kwon, Oh-Bin, Choi, Jae-Hyeog, Park, Chul Soon, Kim, Song-Rae, Moon, Uk Yeol, Cha, Boksik, Choi, Dong Kyu, Lee, Sungwoo, Namkung, Wan, Woo, Joohan, Seo, Yohan

المساهمون: National Research Foundation of Korea

المصدر: Frontiers in Pharmacology ; volume 14 ; ISSN 1663-9812

الوصف: Anoctamin 1 (ANO1), a drug target for various cancers, including prostate and oral cancers, is an intracellular calcium-activated chloride ion channel that plays various physiopathological roles, especially in the induction of cancer growth and metastasis. In this study, we tested a novel compound isolated from Schisandra sphenanthera , known as schisandrathera D, for its inhibitory effect on ANO1. Schisandrathera D dose-dependently suppressed the ANO1 activation-mediated decrease in fluorescence of yellow fluorescent protein; however, it did not affect the adenosine triphosphate-induced increase in the intracellular calcium concentration or forskolin-induced cystic fibrosis transmembrane conductance regulator activity. Specifically, schisandrathera D gradually decreased the levels of ANO1 protein and significantly reduced the cell viability in ANO1-expressing cells when compared to those in ANO1-knockout cells. These effects could be attributed to the fact that schisandrathera D displayed better binding capacity to ANO1 protein than the previously known ANO1 inhibitor, Ani9. Finally, schisandrathera D increased the levels of caspase-3 and cleaved poly (ADP-ribose) polymerase 1, thereby indicating that its anticancer effect is mediated through apoptosis. Thus, this study highlights that schisandrathera D, which reduces ANO1 protein levels, has apoptosis-mediated anticancer effects in prostate and oral cancers, and thus, can be further developed into an anticancer agent.

الإتاحة: https://doi.org/10.3389/fphar.2023.1163970Test

المؤلفون: Giang, Le Thi, Lee, Sion, Seo, Yohan, Cuong, Nguyen The, Tai, Bui Huu, Van Kiem, Phan, Hang, Nguyen Thi Minh, Oanh, Nguyen Thi Tu, Cuong, Pham Van, Ban, Ninh Khac, Park, SeonJu, Nhiem, Nguyen Xuan

المصدر: Fitoterapia ; ISSN:1873-6971 ; Volume:177

مصطلحات موضوعية: Acylated oleanane-type triterpene saponin, Hepatocellular viability, Symplocos cochinchinensis (Lour.) S. Moore, Symplosaponin

الوصف: Four new acylated oleanane-type triterpene saponins, symplosaponins A-D (1-4) were successfully isolated from the leaves of Symplocos cochinchinensis (Lour.) S. Moore, alongside with five known compounds (5-9), 2-methoxy-4-prop-1-enylphenyl-1-O-β-D-apiofuranosyl-(1 → 6)-β-D-glucopyranoside (5), and 1-[O-β-d-xylopyranosyl-(1 → 6)-O-β-d-glucopyranosyl]-2,6-dimethoxy-4-propenyl-phenol (6), 6-O-p-coumaroylsucrose (7), arillatose B (8), and (-)-secoisolariciresinol-O-β-D-glucopyranoside (9). The structures of these compounds were elucidated through spectroscopic methods, comparison with existing data, and chemical methods. Furthermore, all compounds were assessed for their impact on hepatocellular viability using the Resazurin reduction assay. These investigations aimed to explore the potential hepatoprotective properties of isolated compounds. As a result, 1-[O-β-d-xylopyranosyl-(1 → 6)-O-β-d-glucopyranosyl]-2,6-dimethoxy-4-propenyl-phenol (6) and (-)-secoisolariciresinol-O-β-D-glucopyranoside (9) demonstrated statistically significant hepatoprotective activity in a concentration-dependent manner.

العلاقة: https://doi.org/10.1016/j.fitote.2024.106056Test; https://pubmed.ncbi.nlm.nih.gov/38851515Test

الإتاحة: https://doi.org/10.1016/j.fitote.2024.106056Test
https://pubmed.ncbi.nlm.nih.gov/38851515Test

المؤلفون: Tung, Ninh Khac Thanh, Dung, Duong Thi, Kiem, Phan Van, Hang, Dan Thi Thuy, Nhiem, Nguyen Xuan, The, Nguyen Van, Seo, Yohan, Kang, Jong Seong, Tai, Bui Huu

المساهمون: Vietnam Academy of Science and Technology

المصدر: Chemistry & Biodiversity ; volume 21, issue 3 ; ISSN 1612-1872 1612-1880

الوصف: Three previously undescribed compounds named rauvolphyllas A−C ( 1‐3 ), along with thirteen known compounds, 18 β ‐hydroxy‐3‐epi‐ α ‐yohimbine ( 4 ), yohimbine ( 5 ), α ‐yohimbine ( 6 ), 17‐epi‐ α ‐yohimbine ( 7 ), ( E )‐vallesiachotamine ( 8 ), ( Z )‐vallesiachotamine ( 9 ), 16 S ‐ E ‐isositsirikine ( 10 ), N b –methylisoajimaline ( 11 ), N b –methylajimaline ( 12 ), ajimaline ( 13 ), (+)‐lyoniresinol 3α‐ O ‐ β ‐D‐glucopyranoside ( 14 ), (+)‐isolarisiresinol 3α‐ O ‐ β ‐D‐glucopyranoside ( 15 ), and (−)‐lyoniresinol 3α‐ O ‐ β ‐D‐glucopyranoside ( 16 ) were isolated from the aerial parts of Rauvolfia tetraphylla L. Their chemical structures were elucidated based on the extensive spectroscopic interpretation of HR‐ESI‐MS, 1D and 2D NMR spectra. The absolute configurations of 2 and 3 were determined by experimental ECD spectra. Compounds 5 , 6 , 7 , and 11 – 13 exhibited nitric oxide production inhibition activity in LPS‐activated RAW 264.7 cells with the IC 50 values of 79.10, 44.34, 51.28, 33.54, 37.67, and 28.56 μM, respectively, compared to that of the positive control, dexamethasone, which showed IC 50 value of 13.66 μM. The other isolates were inactive with IC 50 values over 100 μM.

الإتاحة: https://doi.org/10.1002/cbdv.202302123Test

المؤلفون: Yen, Duong Thi Hai, Cuc, Nguyen Thi, Tai, Bui Huu, Van Kiem, Phan, Hoang Duc, Manh, Nhiem, Nguyen Xuan, Cho, Su-Hyeon, Jeong, Sung Baek, Seo, Yohan, Park, SeonJu

المصدر: Natural Product Research; Apr2024, Vol. 38 Issue 7, p1120-1126, 7p

مصطلحات موضوعية: TRITERPENOID saponins, BACOPA monnieri, COLON cancer, CANCER cells, LUNG cancer

مستخلص: Using combined chromatographic methods, two new triterpenoid glycosides, bacopasaponin K (1) and bacopasaponin L (2), along with eight known compounds, bacopaside IV (3), bacopaside VII (4), bacopasaponin E (5), bacoside A3 (6), bacopasaponin F (7), bacopasaponin C (8), bacopaside I (9), and bacopaside II (10) were isolated from the methanol extract of the Bacopa monnieri. Their structures were elucidated by 1D-, 2D-NMR spectroscopic analysis, HR-ESI-MS and comparing with the NMR data reported in the literature. All these compounds were evaluated for their cytotoxic activity using the cell counting kit-8 (CCK-8) assay. Compounds 4, 6, 8, and 10 exhibited potential cytotoxic effects against human lung cancer cells (PC9) and human colon cancer cells (SW620). [ABSTRACT FROM AUTHOR]

: Copyright of Natural Product Research is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Lim, Jung A, Seo, Yohan, Choi, Eun-Joo, Kwak, Sang Gyu, Ryu, Taeha, Lee, Jae Hoon, Park, Ki Hyuk, Roh, Woon Seok

المصدر: Medicine ; volume 101, issue 52, page e32597 ; ISSN 1536-5964

الوصف: Lower extremity revascularization (LER) for peripheral artery disease in elderly patients is associated with a high risk of perioperative morbidity and mortality. This study aimed to a conduct retrospective review and propensity score matching analysis to determine whether the use of regional anesthesia (RA) instead of general anesthesia (GA) in geriatric patients undergoing LER for peripheral artery disease results in improved short-term mortality and health outcomes. We reviewed medical records of 1271 patients aged >65 years who underwent LER at our center between May 1998 and February 2016. According to the anesthesia method, patients were grouped in the GA and RA groups. The primary outcome was short-term mortality (7-day and 30-day). The secondary outcomes were 5-year survival rate, intraoperative events, postoperative morbidity, and postoperative length of stay. A propensity score-matched cohort design was used to control for potentially confounding factors including patient demographics, comorbidities, American Society of Anesthesiologists physical status, and preoperative medications. After propensity score matching, 722 patients that received LER under GA (n = 269) or RA (n = 453) were identified. Patients from the GA group showed significantly higher 7-day mortality than those from the RA group (5.6% vs 2.7% P = .048); however, there was no significant difference in 30-day mortality between the groups (GA vs RA: 6.3% vs 3.6%, P = .083). The 5-year survival rate and incidence of arterial and central venous catheter placement or intraoperative dopamine and epinephrine use were significantly higher in the GA group than in the RA group ( P < .05). In addition, the frequency of immediate postoperative oxygen therapy or mechanical ventilation support was higher in the GA group ( P < .05). However, there was no difference in the postoperative cardiopulmonary and cerebral complications between the 2 groups. These results suggest that RA can reduce intraoperative hemodynamic support and provide ...

الإتاحة: https://doi.org/10.1097/md.0000000000032597Test

المؤلفون: Jeong, Sung Baek, Das, Raju, Kim, Dong-Hyun, Lee, Sion, Oh, Hye In, Jo, Sungwoo, Lee, Yechan, Kim, Jeongdong, Park, SeonJu, Choi, Dong Kyu, Moon, Uk Yeol, Kwon, Oh-bin, Namkung, Wan, Lee, Sungwoo, Cho, Byoung Chul, Woo, Joohan, Seo, Yohan

المساهمون: National Research Foundation of Korea, Dongguk University

المصدر: Biomedicine & Pharmacotherapy ; volume 153, page 113373 ; ISSN 0753-3322

مصطلحات موضوعية: Pharmacology, General Medicine

الإتاحة: https://doi.org/10.1016/j.biopha.2022.113373Test
https://api.elsevier.com/content/article/PII:S0753332222007624?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0753332222007624?httpAccept=text/plainTest

المؤلفون: Jeong, Sung Baek, Das, Raju, Kim, Dong-hyun, Lee, Sion, Oh, Hye In, Jo, Sungwoo, Kim, Jeongdong, Park, SeonJu, Moon, Uk Yeol, Kwon, Oh-bin, Namkung, Wan, Lee, Sungwoo, Cho, Byoung Chul, Woo, Joohan, Seo, Yohan

الوصف: Background and Purpose: Anoctamin 1 (ANO1) is a calcium-activated chloride channel found in various cell types and is overexpressed in non-small cell lung cancer (NSCLC), a major cause of cancer-related mortality. This study aimed to investigate whether verteporfin exerts anticancer effects on non-small cell lung cancer by gradually reducing ANO1 protein levels. Experimental Approach: We screened ANO1 inhibitors for biological activity using high-throughput screening and selected verteporfin. Subsequently, we investigated the effects of verteporfin on NSCLC cells as well as the molecular mechanisms involved. Key Results: Verteporfin reduced ANO1 protein levels but had no effects on its channel function. Verteporfin did not inhibit ANO1-induced chloride secretion but reduced ANO1 protein levels in a dose-dependent manner, with an IC50 value of ~300 nM. Verteporfin inhibited neither P2Y receptor-induced intracellular Ca2+ mobilization nor cystic fibrosis transmembrane conductance regulator (CFTR) channel activity. Molecular docking studies revealed that verteporfin bound to specific sites on ANO1 protein. Interestingly, verteporfin decreased p-EGF receptor levels via reduction of ANO1 protein levels; however, it increased p-ERK1/2 levels. Verteporfin reduced the viability and migration of only ANO1-expressing cells, PC9, and gefitinib-resistant PC9 cells, and induced apoptosis by increasing caspase-3 activity and PARP-1 cleavage; however, it did not affect hERG channel activity. Conclusion and Implications: These results indicate that the main mechanism underlying verteporfin action associated with anticancer activity involves the reduction of ANO1 protein levels.

الإتاحة: https://doi.org/10.22541/au.170670406.65118309/v1Test

المؤلفون: Huong, Phan Thi Thanh, Nhiem, Nguyen Xuan, Park, SeonJu, Seo, Yohan, Jeong, Sung Baek, Trang, Do Thi, Yen, Duong Thi Hai, Dung, Duong Thi, Hoa, Truong Thi Viet, Huyen, Le Thi, Lam, Le Doan Tung, Tai, Bui Huu, Kiem, Phan Van

المصدر: Chemistry & Biodiversity; Nov2023, Vol. 20 Issue 11, p1-7, 7p